AffiniPure-VHH™ Secondary Antibodies

Contact Us Introduction Camelid species such as Alpaca and Llama produce a unique class of antibodies composed only of heavy Read More...

Western blotting guide: Part 2, Protein separation by SDS-PAGE

Contact Us Once prepared, the sample proteins are separated by PAGE (polyacrylamide gel electrophoresis), which may be performed under native Read More...

Western blotting guide: Part 1, Introduction and Sample Preparation

Contact Us In 1979, Towbin et al. first detailed the process of immunoblotting – the separation of proteins from a Read More...

Affinity Vs Avidity

Contact Us Affinity and avidity are terms used to describe the strength of the bond between an antibody and its Read More...

Immunoglossary

Contact Us Antibodies are such critical reagents for scientific research that a unique language has been coined relating to them. Here, we’ve Read More...

Serological testing for diagnostics and disease surveillance

Serological tests enable disease surveillance from initial infection through to the development of immunity against infectious diseases. The power of Read More...

Lateral flow tests for diagnostics

Lateral flow assays are one of the many types of immunoassays available for health monitoring. As point of care diagnostic Read More...

Selecting Fluorophores for Antibody-based Research

Fluorophores are essential tools for scientific research. They are widely used for immunoassay techniques such as flow cytometry, immunohistochemistry (IHC), Read More...

An Introduction to Secondary Antibodies

Antibodies are invaluable to scientific research, diagnostics, and therapeutics. These simple yet powerful scientific tools find their way into a Read More...

Solutions for Species on Species Experiments

When using primary antibodies derived from the same species as the sample material (species on species)  non-specific background staining can Read More...

Cross-adsorbed secondary antibodies and cross-reactivity

Immunoglobulins from different species share similar structures. Secondary antibodies raised against one species are likely to recognize epitopes on other Read More...

Blocking WIth Fabs

Blocking: Use of unconjugated Fab fragments to block endogenous immunoglobulins and avoid off target signal   Background staining may be Read More...