Abbkine provides popular and best-selling primary antibodies, covering popular and key research areas, including signaling pathways, neuroscience, cytoskeleton, epigenetics, etc.; all types of antibody display applications have been verified by Abbkine scientists and are authentic and reliable. Antibodies can detect endogenous proteins. lead time: 3-4 days for delivery. Applicable research fields include: cardiovascular research, epigenetics, neuroscience, metabolomics, cancer, cell biology, etc.

Features and Advantages

• The delivery cycle is short and the delivery is fast.
• Strict quality control and quality commitment.
• The experimental results are authentic and reliable.
• It is confirmed by actual literature research results.

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The secondary antibody refers to the antibody used for targeted binding to the primary antibody. In a variety of immunoblotting, ELISA, and IF experiments, the secondary antibody and the primary antibody are used in combination to detect the target protein and enhance the detection signal. The secondary antibody often has a label, and the label of the secondary antibody used for WB detection is usually horseradish peroxidase (HRP).

The secondary antibody products provided by Abbkine include commonly used enzyme-labeled secondary antibodies (such as HRP-labeled secondary antibodies), DyLight fluorescent secondary antibodies, and IFKine™ secondary antibodies (from donkeys and adsorbed by the serum of other species, with lower non-specific binding and background. Particularly suitable for immunological multi-label experiments), IPKine™ secondary antibodies (effectively avoid heavy chain or light chain interference, especially suitable for IP-WB detection).

Features and Advantages

• If the laboratory is equipped with an Odyssey infrared laser imaging system, you can choose a secondary antibody that combines infrared dyes (such as Dylight 680 or Dylight 800), without developing, and direct imaging.
• In the immunoprecipitation (IP) experiment, the detection result of the target protein with a molecular weight of 25 kDa or 50 kDa often encounters the interference of the antibody lgG light chain or heavy chain. Abbkine’s IPKine™ products can effectively solve the problem of light and heavy chain interference and bring you perfect and predictable results.
• Dylight™ fluorescent secondary antibodies and IFKine™ secondary antibodies (donkey source and serum adsorption from other species, lower non-specific binding and background, especially suitable for immunological multi-label experiments).

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Epitope tags are frequently used with recombinant protein from E. coli, yeast or mammalian cell cultures to allow researchers to selectively extract a target protein from the endogenous samples. They can also serve as a universal detection method by using tag-specific antibodies to these
sequences in applications such as Western blot, ELISA, immunofluorescence and immunoprecipitation.
Abbkine epitope tag antibodies include highly specific monoclonal and polyclonal antibodies of the most common tags used by researchers, such as His tag, GST tag, S tag, HA tag, DDDDK tag, Myc tag, MBP Tag and mCherry tag, etc. Abbke also offers a wide selection of conjugated epitope tag antibodies, including Biotin, HRP, AbFluor™ dyes, agarose and magnetic beads conjugates.

Features and Advantages

• Specific—highly specific tag monoclonal antibody enables high yield and high purity of immunoprecipitation
• Low background—stable, pre-blocked beads and specific antibody minimize non-specific binding for immunoprecipitation
• Convenient—product instructions provide an easy-to-follow, optimized protocol for immunoprecipitation in approximately 1-2 hours
• Flexible—available in 100μl, 400μl and 2ml resin package sizes to allow for small and large scale immunoprecipitations
• Versatile — beads are compatible with manual and automated workflows

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Loading control proteins are known to be constitutively and stably expressed at high levels in almost all tissues and cells, who are useful to ensure that an even amount of protein has been loaded across the gel. They also indicate the proper transfer of proteins to the membrane during the western blotting process.

Used to evaluate the efficiency of Western blotting and compare the amount of protein loaded per well in the gel. Abbkine offers a comprehensive portfolio of loading control monoclonal, including popular indicators (such as β-Actin, GAPDH, etc.) and specific species (such as plants, zebrafish, yeast, etc.).

Abbkine offers a comprehensive portfolio of loading control monoclonal and polyclonal antibodies with high specific and multiple applications to meet and satisfy your most types of protein quantity and localization analysis. In addition to common humans, mice, and rats, we also provide more loading control antibodies for other species, as well as bulk customization services.

Features and Advantages

• Abbkine offers a wide range of loading control antibodies, including popular indicators (such as β-Actin, GAPDH, etc.) and specific species (such as plants, zebrafish, yeast, etc.).
• Abbkine offers a wide range of loading control antibodies, including the hot targets and unique species, covering the molecular weights from 15 kDa to 116 kDa.
• Abbkine conjugated loading control antibodies have diverse conjugates, including enzyme and featured fluorescent dyes, including HRP, biotin, FITC, Cy3, Cy5, agarose beads, magnetic beads and AbFluor™ fluorophores (350, 405, 488, 555, 594, 647, 680), with the application of WB, IHC, IF and IP experiments.

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Abbkine focuses on Proteomics and Cytology, and is committed to the innovation and development of various scientific kits, proteins, antibodies and other research tools related to proteomics and cytology, expecting to accelerate the pace of life science research and drug discovery. Here, we present our newly launched selected primary antibodies, which involve hot target research areas, with high quality and bulk stock. Abbkine strongly launched selected primary antibody with high quality control, which will be your first reliable choice. The purification process of Selected Primary Antibody is affinity purification of antibody from mouse ascites or rabbit antiserum by affinity chromatography using epitope-specific immunogen. It is suitable for WB experiment, IHC experiment, IF experiment, IP experiment and ELISA experiment. See specific products for details.

Features and Advantages

• Selection -Our broad selection of validated, published and promised antibodies are targeted to key research areas like cell signaling, neuroscience, cytoskeleton, cancer, and epigenetics.
• Trust-Enjoy complete confidence in your work with proven antibodies from the brands you trust, backed by our quality guarantee.
• Expertise-Our knowledgeable technical support scientists are ready to help you with every aspect of your research, from antibody selection to troubleshooting.
• Our most popular products are listed in this brochure, but you can see our complete selection by visiting Abbkine.com. Check back often – Abbkine is constantly developing new antibodies to novel targets

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Abbkine IFKine™ are series of specially optimized secondary antibodies with improved brightness, photostability and less nonspecific hybridization and background. Donkey host and other species of serum/IgG absorbed make it ideal choice for fluorescence staining, especially in fluorescence multiple labeling. IFKine^TM fluorescence secondary antibodies have high specificity, avoiding the nonspecific hybridization with samples through other species of serum/IgG absorbed. Combined with donkey host, they have good preformance in fluprescence multiple labelling.

Features and Advantages

• Easy to use. Component optimized liquid solution package, easy and convenient.
• Various options. Trail package size available with larger size as well.
• Suitable for fluorscence multiple labelling. Donkey host, compatible with most primary antibodies, easy to block
• Minimum nonspecific reaction. Featured serum absorbtion of other species
• High quality guarantee. High brightness photostability and pH tolerance.

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Featured IPKine™ secondary antibody for Western Blot after Immunoprecipitation 25 kD or 50 kD protein detection on Western blots after Immunoprecipitation is often suffered from heavy or light chain blotting contamination. Abbkine’s IPKine™ products could solve these problems and bring you satisfying results with good performance.

Features and Advantages

• Trail package size available and high cost-performance Less background noise, obtaining cleanest and clearest target band
• Excellent to avoid interference of antibody heavy/light chains Minimized cross-reactivity with IgG of other species as well

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Differentiation cluster (also known as differentiation cluster or simply Cluster of differentiation (CD)) refers to the cell surface molecules that are used for immune antigen recognition. Physiologically, Clusters of Differentiation CD are usually used as important receptors or ligands for cells; In addition, some CD can be used in cell signal cascade (cascade reaction), thereby changing the behavior of the cell; Some CD proteins are not related to cell communication, but have other functions, such as cell adhesion. In conclusion, Clusters of Differentiation CD can be used for many purposes. It is not only involved in the recognition of antigens, capture of antigens, and promotion of the interaction between immune cells and antigens or immune molecules, but also mediates the adhesion between immune cells and between immune cells and matrix, playing an important role in the immune response, activation and effect stages.

Differentiated clusters (also known as differentiated clusters or CD for short) refer to cell surface molecules that are used for identification of immune antigens. Abbkine gives you molecules such as CD24, CD274, CD86, and CD155 to help tumor immune escape & signaling pathway research.

Features and Advantages

• Determined by LAL method, the endotoxin content in each microgram of protein is less than 1.0EU .
• The purity is more than 95%, and each cytokine is equipped with 1 mL of redissolution Buffer which is most compatible with the cytokine, so the product performance is more stable after redissolution.
• Packaging in the form of freeze-dried powder is safe and secure, and there is no need to worry about the quality of the products at hand. The outer packaging adopts EP tube without heat source and endotoxin, which eliminates endotoxin interference from the packaging.

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Cytokine, as a unique molecule for cell signal transduction, as an important role in cell proliferation and development. Abbkine provides Cytokines, including interleukin, chemokine, colony stimulating factor (CSF), interferon and transforming growth factor (TGF) and tumor necrosis factor (TNF) families for cancer, apoptosis, endocrinology, immunology, neuroscience, protease and stem cell research. These cytokines are rigorously validated to meet the developer’s need for high quality, high bioactivity and competitive price.

Features and Advantages

• Determined by LAL method, the endotoxin content in each microgram of protein is less than 1.0EU
• The purity is more than 95%, and each cytokine is equipped with 1 mL of redissolution Buffer which is most compatible with the cytokine, so the product performance is more stable after redissolution.
• Packaging in the form of freeze-dried powder is safe and secure, and there is no need to worry about the quality of the products at hand. The outer packaging adopts EP tube without heat source and endotoxin, which eliminates endotoxin interference from the packaging.

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Cytokine (CK) is a kind of small molecule polypeptide or glycoprotein with wide biological activity synthesized and secreted by immune cells (such as monocytes, macrophages, T cells, B cells, NK cells, etc.) and some non-immune cells (endothelial cells, epidermal cells, fibroblasts, etc.). Cytokines can mediate the interaction between cells and have many biological functions, such as regulating cell growth, differentiation and maturation, maintaining function, regulating immune response, participating in inflammatory reaction, wound healing and tumor growth and decline. Cytokines can be divided into interleukin, interferon, tumor necrosis factor superfamily, colony stimulating factor, chemokine, growth factor and so on.

Abbkine provides you with cytokines, including interleukin, chemokine, colony stimulating factor (CSF), interferon, transforming growth factor (TGF) and tumor necrosis factor (TNF), which are used in cancer, apoptosis, endocrinology, immunology, neuroscience, protease and stem cell research.

Animal-free cytokines from Abbkine cell culture (eukaryotic cells) are produced by using non-animal raw materials and expression system, and the culture media in the expression system are serum-free, animal-free and clear in chemical composition.

Features and Advantages

• Determined by LAL method, the endotoxin content in each microgram of protein is less than 1.0EU
• The purity is more than 95%, and each cytokine is equipped with 1 mL of redissolution Buffer which is most compatible with the cytokine, so the product performance is more stable after redissolution.
• Packaging in the form of freeze-dried powder is safe and secure, and there is no need to worry about the quality of the products at hand. The outer packaging adopts EP tube without heat source and endotoxin, which eliminates endotoxin interference from the packaging.

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Enzymes have specific sequence recognition ability and high-efficiency biocatalytic activity. Under certain conditions, molecules such as nucleic acids and proteins can be cut, connected and modified. At the same time, tool enzymes have mild reaction conditions and excellent biocompatibility. Based on these advantages, reference enzymes have been widely used for the analysis and detection of bioactive molecules such as nucleic acids, proteins and small molecules. Abbkine protein reference enzyme can be used for removing labels of various proteins, and has the advantages of high protease purity, high activity, stable product, simple operation and time saving.

Features and Advantages

• high purity
• high activity
• Easy operation and time saving

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Abbkine offers unique protein purification tools –PurKine™ Protein purification kits, which is an unique product line, along with complete protein fusion tags and anti-tags antibody products, will meet your needs to purify most of different kinds of protein, prepare samples, and to do download test.

Since purification of natural proteins is a challenging task, scientists have developed proteins fusion tags to purify and detect target proteins. These tags include polyhistidine (His), glutathione-S-transferase (GST) tag, HA tag, DDDDK (Flag) tag, Myc tag, MBP tag, and biotin tag and so on .

Features and Advantages

• Provide the Packed Column and buffer using to purify protein
• Easy to use, easy to operate, high purification efficiency.

Abbkine provides ExKine™ protein extraction kits which are capable of extracting high-purity protein without pollution. ExKine™ protein extraction kits can also extract two parts of the protein separately, so it is convenient and efficient.

A good protein is half done in WB experiment. Usually we need to lysate cell and tissue to extract the target protein. Once lysis begins, hydrolysis, dephosphorylation and denaturation start. If we add proper protease/phosphatase Inhibitor Cocktail during the process, these reactions can be slow down and the chances to extract a good protein have increased.

Features and Advantages

• High-purity and no-pollution—the extracted protein has high purity and maintains natural activity, reducing cross-contamination;
• Strong timeliness—non-denatured active protein can be purified within two hours;
• Compatible applications—the extracted protein can be directly used in a variety of subsequent proteomics applications;
• Don’t worry about it later— have special protease inhibitor set, prevent degradation, prevent useless;
• The price is suitable and the quality is high—the price is affordable and the cost performance is super high among the same type of products.

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Abbkine LinKine™ Labeling Kits provide a quick and convenient method to label your proteins/antibodies and peptides with our superior AbFluor™ dyes, FITC, TRITC, Cy3, Cy5, Cy7 or HRP/ AP/ Biotin / Biotin-XX / BSA / KLH and so on .

LinKine™ Labeling Kits, containing everything you need to produce covalently and directly labeled antibodies, proteins and peptides quickly and easily. It only takes 3

simple steps and 30 seconds hands-on time to obtain high quality and covalent conjugates.

Labeled antibodies are compatible with various immunological applications including ELISA/ Flow cytometry/ Western blot/ Immunohistochemistry (IHC)/ Immunofluoresence (IF), and so on. So you can validate your results through multiple platforms using the same antibody.

Features and Advantages

• Easy and Quickly : Easy labeling of antibodies and proteins /peptides with optimized protocol ,
• High efficiency and convenient : Less than 30 seconds hands-on time, with only 3 simple steps to obtain the ready-used labeled and high purified antibody and proteins to do the multiple download experiments , such as ELISA/IHC/FC/IF/WB, etc.
• Multiple choices: Provide different conjugates ,such as AbFluor™ dyes , FITC, TRITC, Cy3, Cy5, Cy7 or HRP/ AP/ Biotin / Biotin-XX / BSA / KLH and so on .

Abbkine newly launched EliKine™ series of ELISA kits exert high sensibility and specificity, with a comprehensive selection of ELISA kits available for the quantification of cytokines, hormones and other proteins, to meet your multiple experiment demands.

Focusing on immunology and cytology research fields, Abbkine scientifc professionals have been involvedin researching, developing and manufacturing highperformance immunoassays and cell research reagents all around the world. We are the trusted and dependable supplier to many international leading research institutes and clinical diagnostic companies.

With original manufacturing of more than 90% of our products, we are continuing the tradition of developing innovative and featured EliKine™ ELISA kits to meet all of our clients’ research needs. Abbkine is constantly developing new ELISA kits that involve cytokines, hematologi-cal factors, hormones, peptides and immunoglobulins to measure a wide range of cytokines across many species.

Features and Advantages

• Specificity: Based on highly specific monoclonal or polyclonal antibody pairs selected for optimal performance.
• Quality: High quality and stability of standards and other ELISA reagents to ensure high analysis quality.
• Expertise: Our technical support scientists are ready to help you with every aspect of your research, from troubleshooting to results analysis.
• Performance: With high sensitivity and accuracy, good repeatability, Abbkine EIiKine ELISA kits offer competitive prices for reseachers.

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Apoptosis is a basic biological phenomenon, which refers to the autonomous and orderly death of genetically controlled cells in order to maintain the stability of the internal environment. It plays a key role while multi-cellular life clear up the  unwanted or abnormal cells. For the normal cells, phosphatidylserine (PS) is located on the inner leaflet of the plasma membrane, while during the early stage of apoptosis, PS is translocated to the outer layer and is exposed on the external surface of the cell. This early event in apoptosis can be detected by using a sensitive method in which to detect PS exposure.

Annexin V is a Ca²⁺-dependent phospholipid binding protein with a molecular weight of 35~36 KDa, which has the highest affinity for phosphatidylserine (PS). Based on its affinity for PS, Annexin V can be utilized as a sensitive probe for cell surface exposure of PS. Early apoptosis can be detected directly while Annexin V labed with different fluorescein or biotin.

Propidium iodide (propidine iodide, PI) is a nucleic acid dye which can not penetrate the intact cell membrane, because the cell membrane of early apoptotic cells is intact and resistant to PI. However, for the cells and dead cells in the middle and late stages of apoptosis, PI can dye the nucleus through the cell membrane. Therefore, using Annexin V with PI can distinguish cells in early, middle and late apoptosis from dead cells. PI can be excited by laser line in 488,532 or 546nm and emit red fluorescence.

According to the early apoptosis detection index: Annexin V、JC-1, mid-term apoptosis detection index: Caspase and late apoptosis detection index: TUNEL, Abbkine can provide you with the Annexin V-AbFluor™ apoptosis detection kit, TUNEL apoptosis detection kit (green/ red fluorescence), Caspase series of activity analysis kit (colorimetric method) to help your scientific research !

Features and Advantages

• 1)Protein level Annexin V series apoptosis kits detect the valgus of phosphatidyl serine (PS): having stronger fluorescence intensity and photostability. Apoptotic cells can be identified and quantified by flow cytometry or fluorescence microscope.
• 2)Mitochondrial membrane potential analysis kit (JC-1): as an indicator for cell health, detecting mitochondrial membrane potential; JC-1 is a fluorescent, lipophilic, cationic dye; red fluorescence represents healthy mitochondria. Green fluorescence indicates mitochondria with poor health.; Optimized for flow cytometry or fluorescence microscopy; contains CCC( potent mitochondrial phosphorous oxide coupling reagent).
• 3)Cell level Caspase series kit is a simple and optimized experimental method to detect the expression of Caspase in apoptosis-related protein Caspase and detect mid-term apoptosis.
• Molecular level TUNEL kits detect late cell apoptosis: exclusively optimized experimental protocol for fluorescent enzyme labeling, fluorescence microscope, flow cytometry, respectively; based on the most widely used FITC channel (Ex/Em =490 nm/520nm), easy to detect

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Cell staining and tracer reagents are important tools for tracking cells in complicated environments and are widely used in cell migration, wound healing and stem cell differentiation research. Cell motion and localization studies require specialized probe dyes which must be non-toxic to living cells, and have a variety of fluorescent colors to choose in order to match different laser wavelengths and filters of different instruments, and also can be co-stained with antibody labeling or other cell analysis marker probes.

Abbkine provides the following kit for your choice, including: Live and Dead Cell Double Staining Kit, Live Cell Tracking Kit (Green Fluorescence), Cell Plasma Membrane Staining Kit, Mitochondrion Staining Kit, Cell Staining Kit F-actin Staining, TraKine™Pro live cell staining kit, Tracer Reagent, etc.

Features and Advantages

• TraKine™ cell staining kit is a set of traditional fluorescence imaging tools using to dye whole cells and organelles (including cell membrane, mitochondria, etc.). it is suitable for dying living cells, fixed cells, suspended cells or attached cells. it can be used in fluorescence microscopy, flow cytometry and other fluorescence platforms.
• Abbkine TraKine™ Pro is a series of long-term super-resolution cell staining portfolio for labeling subcellular structures of live and fixed cells. Unique TraKine™ Pro dyes, combined with super-resolution microscopes (such as SIM, STED, TIRF, STORM and PALM), are perfectly for observing cell dynamics and three-dimensional structure of living cells.

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Biochemical detection refers to the use of biological or chemical methods to detect samples. It is mainly used to determine the diagnostic methods of enzymes, carbohydrates, lipids, protein and non-protein nitrogen, inorganic elements and other biochemical indicators and body function indicators.

According to the detection index, currently Abbkine biochemical assay kits can be divided into several different series: oxidative stress series(oxidant and antioxidant series, glutathione series, anti-stress series), coenzyme&coenzyme factors series(coenzymeⅠ/Ⅱ series), energy metabolism series(ATP series, mitochondrial respiratory chain series), carbohydrates metabolism series(glycolysis series, tricarboxylic acid cycle series, carbohydrate metabolism series, amylum metabolism series), lipid metabolism series(fatty acid metabolism series, esterase metabolism series), nitrogen metabolism series(amino acid metabolism series) ,ion metabolism series and so on.

The types of samples that the biochemical detection kit can detect: serum (plasma), animal and plant tissues, cells, bacteria, fungi, fruit juices and other biological fluids. Abbkine’s biochemical kit provides a complete set of reagents and standards required for biochemical testing, and is accompanied by detailed experimental operation instructions, which can help you quickly obtain accurate and repeatable results, saving time and efficiency.

Features and Advantages

• Provide a complete set of reagents and standards required for biochemical testing.
• With detailed experimental operation instructions, it can help you quickly obtain accurate and repeatable results, saving time and efficiency.

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Cell senescence detection kit (cell senescence) is used for senescence detection of cultured cells and tissue sections. Staining senescent cells only, not staining presenescent cells, quiescent cells, immortal cells or tumor cells.

Abbkine provides different serums from a variety of sources to meet your different experimental needs. Note: The blocking serum cannot contain the target protein and is different from the source of the primary antibody. The blocking serum from the same source as the secondary antibody can be used.

The role of serum is mainly in two aspects:

1. There are some non-specific sites on the tissue that can adsorb proteins, so they are blocked with serum in advance to avoid non-specific adsorption of antibodies when antibodies are added for detection.

2. The blocked serum is usually from the same source as the secondary antibody. The animal’s own antibodies in the serum can bind to the cross-reactive sites in the tissue in advance, otherwise if it binds to the secondary antibody in the subsequent steps, it will cause background.

Features and Advantages

• Provide different sera from a variety of sources. A variety of blocked serums.
• Liquid form product (original solution), no need to dissolve, directly diluted with PBS can be used.
• Low preservative content, with less toxicity/impact on cells and proteins.

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Accurate protein quantification is necessary for WB, which involves research topics in molecular biology, cell biology, biochemistry, developmental biology, and neuroscience. Abbkine protein quantification series products include color pre-stained protein marker.

The color pre-stained protein marker is used to monitor the progress of SDS-PAGE electrophoresis, evaluate the transfer efficiency of PVDF, nylon and nitrocellulose membranes, and measure the approximate molecular weight of the protein.

Features and Advantages

• Optimized formulation for SDS-PAGE and Western blotting.
• It is prepared by using 1X SDS-PAGE loading buffer and can be used directly. Without boiling, diluting and adding reducing agent.
• 6)For gels of different specifications, the recommended loading volume is：Mini-gel, 2-5µL/ well (0.75-1.0mm or 1.5mm). Large gel, 5-10µL/ well (0.75-1.0mm or 1.5mm).

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Abbkine’s Protein Gel Flash Staining Kit, as an excellent alternative to the classic gel Coomassie brilliant blue dyes, is being accepted and loved by more customers around the world.

Accurate protein quantification is necessary for WB, which involves research topics in molecular biology, cell biology, biochemistry, developmental biology, and neuroscience. Abbkine protein quantification series products include WB developing solution ECL (picogram grade and femtogram grade).

The family of SuperLumia ECL substrates is a series of ready-to-use chemiluminescent reagents for the detection of HRP-based Western Blot.

Features and Advantages

• The highest sensitive detection with femtogram level.
• Same quality and performance with affordable price.
• Extremely sensitive at femtogram level, require much less sample and antibodies.
• Intense signal output and long duration.
• Pouring directly onto the blot membrane.
• Bulk size with special price is available upon request.

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Cell staining is an important tool for tracking cells in complex environments and is widely used in cell migration, wound healing and stem cell differentiation studies. Cell movement and localization studies require special probe dyes, which must be non-toxic to living cells, and also have a variety of fluorescent colors to choose from, so as to match different instrument laser wavelengths and filters, and can be labeled with antibodies Or other cell analysis labeled probes co-stained.

Abbkine provides fluorescent dyes with strong light resistance & low toxicity, such as: phalloidin, Cy5, Cy3 dyes, Biotin SE, DAPI, etc.

Features and Advantages

• strong light resistance
• low toxicity

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The perfect protein is half of WB success. We need to lyse cells and tissues to extract the target protein. Once lysis occurs, hydrolysis, dephosphorylation, and denaturation begin. If appropriate proteases or phosphatase inhibitors are added to the lysate, these reactions will be greatly slowed down, and the chance of extracting good protein will increase.

Abbkine protease inhibitors: It is composed of multiple groups which including AEBSF, Aprotinin, Bestatin, E-64, Leu-peptin and Pepstatin A, with broad specificity for cysteine, serine, acid protease and aminopepti-dase. This protease inhibitor cocktail has been opti-mized and tested with mammalian cell and tissue extracts.

Features and Advantages

• Suitable for extraction of mammalian cells and tissue proteins.
• Without EDTA.

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Abbkine featured PurKine™ isolation and purification portfolio with complete anti-tag antibodies and synthetic peptides to meet and satisfy your most types of protein purification, sample preparation and detection analysis.

PurKine™ Purification resin/prepacked spin column/kit formats (Large capacity, high purification efficiency)

PurKine™ provide His, GST, MBP, Biotin, ProteinA, G, A/G, L and other protein and antibody purification packings, pre-packed columns and kits, which are simple and efficient.

Features and Advantages

• Ni-IDA: 3 coordinations, forms a relatively stable planar structure, and has the characteristics of high load and high cost performance (soluble protein).
• High performance-excellent bonding ability.
• Cost saving-the same resin can be reused for more than five times, and the performance will not decrease.
• Flexible use—provide multiple forms of products, resin fillers, pre-packed columns and complete kit products.

Cytokines are a kind of unique cellular signal transduction molecules which play an important role in cell proliferation and development. the cytokines Abbkine provide cytokines including interleukin, chemokines, colony-stimulating factors (CSF), interferon, and the family of transforming growth factors (TGF) and tumor necrosis factors (TNF) for cancer, apoptosis, endocrinology, immunology, neuroscience, proteases, and stem cell research. these cytokines are rigorously validated to meet the high quality, high bioactivity and competitive prices demand of scientists.

Abbkine provides you cytokines including interleukin, chemokines, colony-stimulating factors (CSF), interferon, and the transforming growth factor (TGF) and tumor necrosis factor (TNF) family.

Features and Advantages

• High purity, low endotoxin level;
• Full quality control;
• The biological activity is tested;
• Lyophilized powder;

In all stages of cell growth, proliferation, migration and apoptosis, cells are strictly regulated by many factors, which is a complicated and dynamic process. Multi-parameter analysis is very important for accurate detection of this process. At the same time, understanding the mechanism of cell death and survival is also a key content in toxicology analysis and drug discovery. Abbkine provide various detection methods, including: Caspase activity detection; cell counting, cell proliferation, cell senescence, TUNEL apoptosis; membrane change detection (Annexin V staining, membrane potential, mitochondrial functional analysis); cell cytotoxicity analysis, etc.

CCK-8、LDH、EdU cell proliferation/toxicity detection kit ; Annexin V、TUNEL、Mitochondrial Membrane Potential, Caspase and other cell Apoptosis Detection kit and cell senescence staining kit.

Features and Advantages

• CCK-8 kit (cell proliferation / toxicity detection)—— easy to use, only need 3 steps, less than 15 minutes; much higher sensitivity than MTT、XTT、MTS and WST-1; very low cytotoxicity, cells can be used in downstream experiments; in no need of organic solvents or isotope;
• LDH toxicity analysis kit (cytotoxicity)—— determine the LDH,LDH released by damaged cells can formulate pyruvate and NADH by catalyze lactic acid, and NADH can transfer tetrazolium salt MTT to formazan by electronic carrier. The transfer form showed maximum absorption at 565 nm and the absorbance was proportional to the amount of LDH.
• Cell proliferation EdU detection kit (cell proliferation)—— no need to use antibodies, easy to operate; no DNA denaturation, maintain cell morphology and DNA integrity; simple, efficient, finish within 30 minutes; high sensitivity in detection .
• Mitochondrial membrane potential detection (JC-1, apoptosis) was optimized for fluorescence microscope and flow cytometry. When using fluorescence microscope to observe cells, adherent cells did not need trypsin digestion which maintain cell morphology and integrity. Contains positive control CCCP which induce the decrease of mitochondrial membrane potential
• TUNEL apoptosis detection (apoptosis) was optimized for fluorescence microscope and flow cytometry. It is suitable for adherent cells, suspended cells, paraffin embedded tissue sections and frozen sections.
• Caspase activity detection kit (apoptosis)—— quick and convenient operation, can read the value only in three steps, and can measure the enzyme activity directly by the standard curve.
• Cell senescence detection kit (cell senescence) is used for senescence detection of cultured cells and tissue sections. Staining senescent cells only, not staining presenescent cells, quiescent cells, immortal cells or tumor cells

Organelles are microorgans with specific morphological structures and functions in cells, usually wrapped separately by phospholipid bilayers. Similar to organs of human body, organelles are functional units specialized in normal cell operations, whose functions are range from producing cells to control cell growth and reproduction. Organelle extraction and enrichment simplify proteomic analysis, and can help us to deeply understand some diseases related to cell function combined with protein metabolic function analysis Abbkine provides the following organelle separation kit, including nuclear extraction kit and mitochondrial extraction kit.

Abbkine provide complete & active & convenient nuclear extraction kit and mitochondrial extraction kit.

Features and Advantages

• Nuclear extraction kit (Crude formulation): rapid crude extraction (within 1 h), suitable for massive samples and time saving need; delicate extraction method: no cell membrane and cytosol pollution, maintain complete nuclear morphology;
• Mitochondrial extraction kit: highly purified mitochondria can be isolated and most of the mitochondria has complete intima and outer membrane, also having the normal physiological function.

Cell staining and tracer reagents are important tools to track cells in complicated environments and are widely used in cell migration, wound healing and stem cell differentiation research. Cell motion and localization studies require specialized probe dyes which must be non-toxic to living cells, and have various fluorescent colors to choose n order to match different laser wavelengths and filters of different instrument. And also can be co-stained with antibody labeling or other cell analysis marker probes.

Abbkine provide the following kits at your choice, including: live / dead cell double staining kit, live cell tracer kit (green fluorescence), cell membrane staining kit, mitochondrial staining kit, cell staining kit（F-actin staining）, TraKine™Pro live cell staining kit, tracer reagent, etc.

Provide you with high-quality cell staining and tracer reagent.

Features and Advantages

• Live / dead cell double staining kit: one step staining method can distinguish living and dead cells at the same time. It is easy to operate to quickly quantify cell activity, and can be used for fluorescence microscope and flow detection.
• Live cell tracer kit: excellent fluorescent dye can achieve long-term cell tracer; fluorescent probe will not transfer from one cell to the other adjacent cells after labeling, but only transfer to progeny cells.
• Cell cycle detection kit: based on nuclear (DNA) dyes, when they bind to DNA in cells, they produce fluorescence signals. The fluorescence intensity is proportional to the DNA of cells. It provides a convenient and accurate analysis plan for analyzing the percentage of cells in each phase of cell cycle
• TraKine™ series cell staining kits (cell membrane staining, mitochondrial staining, F-actin staining): optimized for various fluorescence analysis platforms with wide applicability, such as fluorescent microporous plate analysis, flow cytometry and fluorescence microscopy.
• TraKine™ Pro live cell staining kit: almost no cytotoxicity; fluorescence can last several hours in living cells, stable and lasting which is an ideal choice for monitoring living cell dynamic; Especially suitable for the study of living cell research under Confocal laser scanning microscopy (Confocal) and long-term super-resolution microscope, it is an ideal choice to observe cell dynamics in three-dimensional space.
• AbFlour™ new generation fluorescent dyes: AbFluor™ dyes have excellent core structure, stable reaction groups, good photostability, which ensure the high stability and high labeling efficiency of biological coupling; broad fluorescence spectrum range, covering ultraviolet, visible and near infrared, to meet most of the experimental needs from researchers; good pH tolerance, remain stable within the pH 2-11.

Cell metabolism is a general term for a series of ordered chemical reactions that occur in cells to maintain life. Its produced products are involved in cell apoptosis, signaling, immune response, metabolic regulation, gene expression and so on, which are closely related with aging, metabolic dysfunction, metabolic disorders and other diseases. The measurement of cell metabolic activity can be used as an important index in cell research. Abbkine CheKine™ cell metabolomics product line includes a large number of related metabolites, enzymes, nutrition and metabolite quantitative detection kits, etc.

CheKine™ series cell metabolism detection kit adopts the optimal reaction principle, its components are simple, its operation is convenient, also having high sensitivity and don’t be disturbed by other substances, its accuracy and specificity are higher.

Features and Advantages

• The components are simple and convenient to operate.
• High sensitivity and free from the interference of other substances
• Higher accuracy and specificity;

Accurate protein quantification is necessary for WB, which involves research topics in molecular biology, cell biology, biochemistry, developmental biology, and neuroscience. Abbkine protein quantification series products include protein quantification kits (BCA assay and Bradford assay), WB developing solution ECL (picogram grade and femtogram grade), color pre-stained protein marker, etc.

Protein quantification series products include protein quantification kits, WB developing solution ECL, color pre-stained protein marker, etc.

Features and Advantages

• Protein quantification kits (BCA assay and Bradford assay)- High sensitivity: the detection line is as low as 25ug/ml, and the minimum detection protein amount is 0.5ug; excellent linearity: the range of linear standard curve: 50-1000 ug/ml; good stability: compared with the method of dye binding, it can detect different proteins The coefficient of variation of the molecule is smaller.
• HRP substrate kits- Extremely sensitive at femtogram level, require much less sample and antibodies. Intense signal output and long duration.
• Protein Gel Flash Staining Kit- The kit does not contain any toxic and pungent substances, which reduces health risks and is more environmentally friendly; the staining process is rapid and highly sensitive. On 1mm thick 10% SDS-PAGE, 1 μg BSA bands are clearly visible within 5 minutes of staining. The 100 ng BSA band was clearly visible within 20 minutes.

For analyzing individual proteins and protein complexes, identifying the interactions between proteins, proteins and nucleic acids, protein purification is the cornerstone of these studies. Since the purification of natural proteins is a challenging task, scientists have developed an experimental system that uses tag fusion proteins to capture, purify and detect the target protein. The most effective way to purify a protein is affinity chromatography, which achieves the purpose of purification by specific binding of the target protein to a matched immobilized ligand. The most notable feature is that the combination of a well-characterized tag and its corresponding solid-phase coupled ligand can achieve affinity purification of the labeled protein in a single step. Antibodies with fusion tags are also widely used in downstream detection and experiments, eliminating the need to develop corresponding probes for each specific recombinant protein.

Antibody purification mainly involves the selective enrichment or specific separation of antibodies from serum (polyclonal antibodies), ascites, and hybridoma cell line culture supernatants (monoclonal antibodies). Protein A, Protein G and Protein L are three bacterial proteins, which are well known for their efficient binding to antibodies. These proteins are recombined and expressed through genetic engineering editing, and are usually used for affinity purification of key antibody types from a variety of different species.

For protein purification and antibody purification, there are three product types: filler, prepacked spin column and kit to meet different research needs.

Features and Advantages

• Protein purification products: contains His, GST, MBP, Flag, Biotin, Strep II-Tag tag purification. (High load-each milliliter of resin can bind to excess tag fusion protein; multifunctional-purification operating conditions: natural or denatured; Cost-effective-the same resin can be reused more than five times, with the same performance; gentle reaction-retention of the target protein Function and antigenicity; Flexible choice-multi-form product selection: resin filler, pre-packed column, complete kit; Simple operation-kit products provide various pre-configured buffers.)
• Antibody purification products: including Protein A, Protein G, Protein A/G, Protein L. (High capacity—each milliliter of resin can bind to an excessive amount of mg of immunoglobulin; high performance—through genetic engineering methods to remove other non-essential domains and reduce non-specific adsorption; Cost-effective—the same resin can be reused five times The above, and the performance is not reduced; Flexible use—multiple product choices, resin fillers, pre-packed columns, complete kits; Simple operation—kit products provide various pre-configured buffers.)

LinKine™ Labeling Kits provide fast conjugation of biomolecules (antibodies, proteins and peptides, etc.) with proteins or enzymes, including HRP、Biotin、FITC、Cy3、AbFluor™, which is based on available amine groups. Directly labeled primary antibodies are advantageous as they eliminate the need for secondary reagents in immunoassay procedures, thus removing a tedious extra cycle of incubation and wash steps in applications such as ELISA and Western blotting. Labeled antibodies are compatible with various immunological applications including flow cytometry, western blotting, and immunofluorescence microscope

AbFluor™ dyes are a series of highly water-soluble fluorescent dyes spanning full UV-visible and near-infrared spectrum for labeling biomolecules, especially proteins and nucleic acids. Their hydrophilic property makes the protein conjugation readily performed in aqueous media, minimizing the use of organic solvents. AbFluor™ dyes not only have much better labeling performance than other fluorescent labeling dyes such as FITC, TRITC, Cy® dyes, IRDye, Alexa Fluor® and Dylight® labeling dyes, but also far more stable than the antibodies or other biomolecules they label. The fluorescent dye in the kit has been pre-activated and can be used directly for coupling experiments.

Directly label enzymes, biotin and fluorescent dyes on antibodies, proteins, peptides or other biomolecules to meet the needs of scientific research, drug development and diagnostic kit development.

Features and Advantages

• Optimized scheme: follow the standard experimental procedure, with excellent pre-activated dye, protein ratio and patented solution formula to obtain the best activity or fluorescence.
• Simple operation: It can be achieved in three steps, and the coupling group is bound to the primary amine site of the antibody or other protein.
• Customization: changing the molar ratio, reaction buffer, pH, and other parameters can achieve different levels of coupling and activity.
• Efficient purification: The kit includes a purification column to ensure rapid and effective removal of unreacted dyes and excellent protein/antibody recovery.

Protein detection-selected important research targets, strict quality control at different levels, and direct spot access

Antibody is a kind of immunoglobulin that can specifically bind with antigen. Antibody is a practical tool for immunological experiments, such as Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF) and Flow Cytometry, FCM). The antibodies provided by Abbkine for protein detection include internal reference antibody, tag antibody, primary antibody, secondary antibody and sealed serum.

The antibodies provided by Abbkine for protein detection include internal reference antibody, tag antibody, primary antibody, secondary antibody and sealed serum.

Features and Advantages

• Loading control antibody-Used to evaluate the efficiency of Western blotting and compare the amount of protein loaded per well in the gel. Abbkine offers a comprehensive portfolio of loading control monoclonal, including popular indicators (such as β-Actin, GAPDH, etc.) and specific species (such as plants, zebrafish, yeast, etc.).
• Epitope & Tag antibodies- Abbkine epitope tag antibodies include highly specific monoclonal and polyclonal antibodies of the most common tags used by researchers, such as His tag, GST tag, S tag, HA tag, DDDDK tag, Myc tag, MBP Tag and mCherry tag, etc. Abbke also offers a wide selection of conjugated epitope tag antibodies, including Biotin, HRP, AbFluor™ dyes, agarose and magnetic beads conjugates.
• antibodies- Abbkine provides popular and best-selling primary antibodies. Target to multiple research areas containing well known signaling pathway, neuroscience, cytoskeleton, cancer, epigenetics. Applications of all antibodies have been tested by Abbkine scientists. All types of antibody display applications have been verified by Abbkine scientists and are authentic and reliable.
• secondary antibody -The secondary antibody refers to the antibody used for targeted binding to the primary antibody. In a variety of immunoblotting, ELISA, and IF experiments, the secondary antibody and the primary antibody are used in combination to detect the target protein and enhance the detection signal. The secondary antibody often has a label, and the label of the secondary antibody used for WB detection is usually horseradish peroxidase (HRP).
• Normal Serum- Normal serum is applied to blocking of samples in Immunohistochemistry(IHC) or Immunofluorescence(IF) experiments, which is strongly recommended as a blocking reagent to reduce background from non-specific, conserved sequence and/or Fc receptor binding. Normally, blocking is done with normal serum from the same species that the secondary antibody was raised in.

Protein extraction is the starting point for immunology/cytology experiments. Abbkine ExKine™ series of protein extraction products include total protein extraction, total membrane protein extraction, cytoplasmic protein extraction, nuclear protein extraction, etc. The separated protein can not only be used to study the location of the protein in the cell, but also can be used for downstream functional research, Such as transcription regulation, signal transduction, enzyme activity analysis, etc.

Features and Advantages

• High purity-The extracted protein has high purity and maintains natural activity, reducing cross-contamination.
• Strong efficiency-Purified non-denatured active protein within two hours.
• Compatible applications-The extracted protein can be directly used in subsequent proteomics applications.
• Super high guarantee-Have a special protease inhibitor set to prevent degradation.
• Good price and high quality-It is affordable in the same type of prod-ucts and the cost performance is extremely high.