Antibodies form a powerful tool used by the immune system to protect us from various pathogens. However, antibodies can also have deleterious effects, as very widely demonstrated by type I hypersensitivity reactions, in particular what we commonly refer to as “allergies”.

Be it to pollen, bee stings or various foods – allergies are extremely common, with various theories as to why they appear to be on the increase.

To cater to researchers investigating allergies, we offer a range of antibodies against common allergens. Many of these antibodies have been shown to compete with naturally occurring IgE – the principal antibody isotype mediating allergies. We offer these antibodies as human IgE (along with antibodies against IgE itself) for use as calibrators and positive controls.

Coronaviruses are a group of viruses at the source of several recent outbreaks, including SARS (Severe Acute Respiratory Syndrome) in 2003, MERS (Middle East Respiratory Syndrome) in 2012 and most recently the outbreak of COVID-19, the illness caused by the SARS-CoV-2 virus. Our collection of anti-coronavirus antibodies was largely obtained from B-cell sequencing of human patients. In addition to the original human IgG1 antibodies, recombinant antibody technology has enabled the production of various engineered formats designed to open up new experimental possibilities for in vitro and in vivo use.

This year, the anti-SARS clone CR3022 was shown to have high affinity for the then-termed 2019-nCoV. The clone is now available in our catalog in formats such as IgM, IgA, antibody fragments, and IgG in different species and isotypes.

The Absolute Antibody team is working hard to support coronavirus research and therapeutic development by supplying related reagents to customers worldwide, as well as providing antibody engineering and manufacturing services. We continue to add new reagents to our catalog, including recombinant anti-human immunoglobulin antibodies for COVID-19 diagnostics and ACE2 Fc fusion proteins.

Absolute Antibody offers a range of recombinant antibodies against viral antigens. The antibodies were initially generated either through classic hybridoma technology, phage-display technology, or sequencing of (immortalized) B-cells from infected human patients. In particular we offer a range of antibodies generated by Humabs BioMed SA from real human patients using their Cellclone technology.

Our range of recombinant antibodies against epitope tags includes classic clones like 9E10 (anti c-myc) offered in multiple species and isotypes to suit your experiment.

Bispecific antibodies are an exciting class of therapeutics permitting simultaneous engagement of two distinct targets with the same antibody. Knob-into-hole (KIH) bispecific antibodies are playing an important role in drug development, as this pioneering format enables heavy-chain heterodimerization and the creation of an IgG-like antibody with different targets on each arm. However, researchers have lacked syngeneic mouse models for evaluating KIH bispecific combinations, as the mutations applied to human IgG1 cannot be readily transferred to murine IgG subtypes.

To address this, Absolute Antibody has developed the first commercially available platform for the generation of fully murine KIH bispecific antibodies. Customers can purchase bispecific antibodies from our catalog off-the-shelf, or mix-and-match targets from the catalog to build their own custom reagent, to enable easier evaluations of potential bispecific combinations in mouse models.

Highlights of our murine KIH bispecific antibodies include:

• One IgG ‘arm’ uses a single-domain binder to overcome the light-chain pairing problem
• Our proprietary Fc Silent™ domain abrogates Fc gamma receptor and C1q binding without affecting FcRn-mediated recycling
• Fully murine backbone for low immunogenicity in mouse models
• Low endotoxin levels and high purity for in vivo research
• Our T-cell-engaging CD3ε-bispecific antibodies have demonstrated success
  killing tumor cells in mouse models

For more information, download our research poster to learn about the generation of our KIH bispecific antibody platform and review functional characterization of our anti-mCD3ε:TRP-1 bispecific antibody, capable of selectively recruiting T-cells to TRP-1+ cancer cells for increased cytotoxic effector function. You can also read a customer case study about researchers at the Leiden University Medical Center who used two of our bispecific antibodies in a study that showed oncolytic viruses in combination with CD3ε-bispecific antibodies could improve the immunotherapy treatment of solid cancer tumors.

In addition to our KIH bispecific antibodies, further murine bispecific antibody constructs are available in an IgG-dAb format, enabling bivalent engagement of each target and representing further antibody engineering options available to our customers. To learn more about our suggested initial go-to formats for bispecific antibodies, as well as the utility of mouse bispecifics in basic research and drug discovery.

View our off-the-shelf bispecific antibodies below, and follow the links to place your order. Please contact us to discuss creating a custom bispecific reagent, using either clones from our catalog or your own antibodies. Starting with a hybridoma cell line, purified antibody or antibody sequence, we can deliver milligram-to-gram quantities of your custom bispecific, 100% royalty-free.

Available Bispecific Antibodies:

DNA and RNA are often thought of as mere information storages; however, the ability of nucleotide sequences to fold into defined structures has allowed nature to exploit these as catalytic and regulatory elements as well.

As our understanding of DNA- and RNA-biology continues to grow, new research tools are required to help us understand the impact on diverse branches of research such as cancer, immunology, aging and infectious disease. At Absolute Antibody, we offer a variety of recombinant engineered antibodies targeting nucleotide structures, in particular DNA and/or RNA primary, secondary and tertiary structures.

Our DNA/RNA research collection includes antibodies for classic structures, including single- or double-stranded DNA, dsRNA, hairpin DNA and Z-DNA, as well as antibodies for more recently discovered structures such as DNA/RNA hybrids, G-quadruplexes and i-Motif. Moreover, we are also featuring our collection of antibodies against naturally occurring or chemically induced modified nucleotide bases. All antibodies are recombinantly produced for ensured purity and batch-to-batch reproducibility.

Each antibody clone is available in a variety of species, isotypes and subtypes designed to increase experimental flexibility and antibody compatibility. For example, you can select a format to enable easier co-labelling studies or increase compatibility with a secondary reagent. You can also add a custom conjugation to allow for easier detection, or select a monomeric binder such as a Fab fragment or half-antibody to improve localization in super-resolution methods and maintain single-domain binding characteristics.

Anti-F4/80 [Cl:A3-1] antibody is the most widely publicized and referenced mouse macrophage and microglia marker, making it a valuable tool for research.

At Absolute Antibody, we have sequenced the original hybridoma (rat anti-mouse F4/80 clone Cl:A3-1; Gordon and Austyn, 1981) and produced recombinant and chimeric anti-F4/80 clone Cl:A3-1 versions, allowing greater experimental flexibility and control for the end user (See Why Go Recombinant?).

• We are the ONLY commercial manufacturer of recombinant F4/80 [Cl:A3-1] monoclonal antibody.
• We are the ONLY commercial manufacturer of recombinant chimeric mouse anti-mouse F4/80 [CI:A3-1] monoclonal antibody, allowing experimental flexibility never allowed before.
• We are the ONLY commercial manufacturer who offers recombinant rat anti-mouse and chimeric mouse anti-mouse F4/80 Clone CI:A3-1 antibodies with an effector-silent Fc domain (Fc SilentTM), allowing greater versatility and confidence for in vivo and in vitro applications.
• Our proprietary AbAb recombinant platform allows us to manufacture chimeric versions of the F4/80 [Cl:A3-1] antibody, allowing you to ‘species switch‘ the antibody’s Fc domain (i.e., change the species of the Fc domain to rat, mouse, human, etc.), while still retaining the antibody’s original species and antigen specificity and sensitivity.
• We offer mouse, rat, human, and hamster un-engineered (wild-type) Fc and Fc SilentTM domains.

F4/80 is a mature mouse cell surface glycoprotein and is a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. High level of expression is found on most tissue macrophages including the spleen, liver kupffer cells, microglia in the brain and skin Langerhans cells.

Absolute Antibody recombinant mouse anti-mouse and rat anti-mouse F4/80 Clone Cl:A3-1 are supplied in purified, azide-free, and low endotoxin versions to allow for in vivo applications.

If you cannot find F4/80 in the format you are looking for or would like to obtain a quote for bulk orders, please just get in touch.

Negative controls are essential to conducting thorough and meaningful experiments. Antibodies can bind to other molecules, especially other proteins, in non-specific or undesired ways. To show that identification of a particular band on a Western Blot or staining for a cell-surface marker in flow cytometry using an antibody is specific, a negative control may be used which should not result in similar band patterns or staining.

Isotype Controls

Isotype controls are a particular type of negative control that feature the same Fc region as the antibody the actual experiment is being conducted with but a variable region that should not bind antigen in the experiment. The Fc region can interact specifically with Fc receptors or complement; hence, it is necessary to control for this “background” when staining for cell-surface markers. These interactions may even be across species; e.g., rat IgG with mouse Fc receptors. Therefore, a diverse panel of isotype controls was traditionally required when performing flow cytometry. See also our Fc Silent™ product range for practical solutions to this problem.

Standardized Controls Offered by Absolute Antibody

Antibody engineering makes possible the generation of a standardized panel of isotype controls with the same “specificity” (i.e., derived from the same original antibody) – this can reduce experimental complexity. The panels benefit from the superior reproducibility and purity we obtain using our recombinant production platform.

Absolute Antibody currently offers isotype-control panels derived from four different antibodies: anti-NP, anti-fluorescein, anti-beta galactosidase (from E. coli) and an antibody called MOPC-21 with unknown specificity. All are available in human, mouse, rat, rabbit and hamster isotypes.

Don’t see the format you need? Just contact us and we’ll be happy to help you.

Tumor antigens serve as important tumor markers and can be used as targets in the detection and treatment of cancer. Antibodies targeting these tumor markers may have diagnostic applications in detection of the presence, type and stage of cancer. They also form an important class of therapeutic antibodies.

Absolute Antibody offers a large catalog of 75+ tumor marker antigens, including anti-TAA (tumor associated antigens) and anti-TSA (tumor specific antigens). We have used our recombinant antibody technology and antibody engineering to generate different engineered formats of these antibodies for all your experimental needs.

Highlights of our tumor marker antigens include:

• Engineer antibodies into any species and with any effector function
• Fc Silent™ formats help to eliminate antibody directed cytotoxicity (ADCC) effector function of Fc receptors
• Create bispecific antibodies on human or mouse background
• Recombinantly produced to ensure batch-to-batch reproducibility
• Low endotoxin levels and high purity for in vivo and in vitro research

View all of our off-the-shelf tumor marker antigens below and follow the links to place your order. If you would like to discuss creating a custom engineered antibody, please contact us. We are always happy to engineer our antibodies to fit your research needs. As we say – If you can describe it, we can make it.

Tumor suppressor proteins are crucial for maintaining cell balance and curbing uncontrolled growth and studying them and their possible loss-of-function is key in advancing cancer research. When tumor suppressors are inactive, cancer can proliferate. Antibodies serve as vital tools in understanding tumor suppressor proteins, allowing precise detection and localization in experiments. Absolute Antibody is committed to supporting scientific progress by offering antibodies tailored for tumor suppressor research. By leveraging these antibodies, researchers gain a refined toolkit to explore and manipulate tumor suppressor functions, paving the way for innovative cancer therapies and contributing to the broader landscape of oncological research.

Key Tumor Suppressor Proteins

P53

The p53 tumor suppressor protein regulates cell growth and prevents the formation of tumors. It acts as a “guardian of the genome” (Park et al., 2016), overseeing DNA repair and, when necessary, triggering apoptosis to eliminate damaged cells. P53 antibody studies are vital because they allow researchers to investigate the protein’s levels, activity, and interactions with precision. Understanding p53’s role and dysregulation in cancer is essential for developing targeted therapies and improving overall strategies for cancer treatment.

Anti-p53 [PAb421]

Immunofluorescence staining of fixed A431 with anti-p53 antibody PAb421 (Ab00142). Published applications of this clone are IHC-P, in vivo, IP, WB, ELISA, IF, and IHC-Fr.

Anti-p53 (a.a. 16-25) [X77]

Immunofluorescence staining of A431 cells with anti-p53 (a.a. 16-25) (Ab02776) X77. Published applications of this clone are IP, WB, ELISA, and IF.

Anti-p63 [BU5]

Immunofluorescence staining of A431 cells with anti-p63 (Ab00871) BU5. Published applications of this clone are FC and IF.

TP63

TP63, a member of the p53 tumor suppressor gene family, encodes for the p63 protein, which regulates epithelial development and maintains genomic stability (Melino, 2011). Unlike p53, TP63 is not commonly associated with direct tumor suppression but is pivotal for controlling cellular differentiation and maintaining epithelial integrity. Multiple promoters and splice variants give rise to various isoforms of this protein. Studying TP63 in cancer research using antibodies is important for understanding its expression patterns, isoform-specific functions, and potential involvement in various cancers. TP63 antibody assays give researchers insight into the roles of TP63 in normal and aberrant cellular processes and thus its potential implications in cancer development and progression. TP63’s distinct functions in epithelial biology differentiate it from traditional tumor suppressors, making it a subject of interest for researchers investigating its nuanced contributions to cancer biology.

P16

The p16 tumor suppressor protein, encoded by the cyclin-dependent kinase inhibitor 2A (CDKN2A) gene, regulates the cell cycle by inhibiting the activity of cyclin-dependent kinases, specifically CDK4 and CDK6, which are involved in promoting cell cycle progression. In inhibiting these kinases, p16 helps prevent uncontrolled cell division and suppresses tumor formation (Li et al., 2012). Unlike p53, which is involved in a broader range of cellular responses, p16 is more specifically focused on cell cycle regulation. Its importance in cancer research is underscored by its frequent inactivation or loss in various cancers, leading to unrestrained cell proliferation. A highly defined P16 antibody is an essential tool in studying p16 as it enables researchers to assess expression levels, localization, and interactions, providing crucial insights into p16’s role in maintaining cell cycle control and its implications for cancer development and treatment. Our recombinant anti-p16 antibody is based on the clone 30859-S17 which has been cited for use in western blot, ELISA, and IHC applications. Additionally, we have engineered this clone from its original mouse IgG1 version into rabbit IgG to decrease cross-reactivity and background signal in human tissues.

KRAS

KRAS mutations, specifically G12V, G12C, and G12D, are biomarkers associated with various cancers, particularly colorectal cancer and non-small cell lung cancer (Gao & Shen, 2022). These mutations result in the constitutive activation of the KRAS protein, leading to uncontrolled cell growth and tumor progression. Studying KRAS G12V, G12C, and G12D in cancer research using antibodies is crucial for understanding their expression patterns, localization, and activity levels. Like inactivated tumor suppressors, KRAS mutations represent an oncogenic driver, promoting tumorigenesis, and KRAS protein antibodies play a pivotal role in dissecting the aberrant signaling pathways associated with KRAS mutations, providing essential information for the development of targeted therapies. One such KRAS antibody is the anti-p21Ras clone DWP which has been cited for use in western blot, ELISA, and IHC and recognizes the KRAS mutant peptides G12V and G12C. Our recombinant version has been engineered from the original mouse IgG2b to such formats as mouse IgG2b Fc Silent™ with abrogated Fc binding for reduced non-specific staining. Other formats include human IgG1 with and without Fc binding functionality, and rabbit IgG.

TGF-beta

Transforming Growth Factor-beta (TGF-β) is a multifunctional cytokine that plays a dual role in cancer, acting as both a tumor suppressor and a promoter, depending on the context (Seoane & Gomis, 2017). In its role as a tumor suppressor, TGF-β helps regulate cell growth, inhibit cell proliferation, and promote apoptosis. However, during later stages of cancer progression, TGF-β can paradoxically stimulate tumor growth and metastasis. Studying TGF-β in cancer research using antibodies is essential for elucidating its complex role in tumorigenesis. Antibodies enable researchers to investigate the levels, activity, and interactions of TGF-β, providing critical insights into its dynamic functions and potential as a therapeutic target in cancer treatment.

Other Recombinant Engineered Tumor Suppressor Antibodies

Cadherin-1: Cadherins, particularly cadherin-1 (or E-cadherin) act as tumor suppressors by preserving cellular adhesion. Anti-cadherin antibodies are instrumental in researching tumor suppressors, allowing specific detection and quantification of cadherin levels, uncovering insights into mechanisms driving tumor progression.

Retinoblastoma protein: Retinoblastoma protein (Rb) is coded for by the RB1 gene, the first described human tumor suppressor gene (Berry et al., 2019), making Rb a pioneer protein for the study of tumor suppressors. Its importance in research has not dwindled, as Rb is central to cell cycle regulation and its downregulation and inactivation can lead to uncontrolled cell growth. We offer the recombinant engineered version of the anti-RBBP5 clone RAB-C379, cited for use in immunoprecipitation mass spectrometry and immunofluorescence assays.

SMARCA4: SMARCA4, also known as BRG1, is a tumor suppressor involved in chromatin remodeling. Mutations or loss of SMARCA4 function are observed in various cancers, notably small cell carcinoma of the ovary. Anti-SMARCA4 antibodies play a crucial role in studying this tumor suppressor, enabling specific detection and quantification, and providing insights into its contribution to cancer development. The anti-Brg1 clone 4E5 has published applications in immunoprecipitation, western blot, immunofluorescence, and immunohistochemistry. We recombinantly engineered anti-Brg1 [4E5] for more experimental options into various human, mouse, and rat IgG isotypes, including Fc Silent™ options on some formats for tailed effector function.

Catenin beta-1: Catenin beta-1 (encoded by CTNNB1), also known as β-catenin, is a key player in the Wnt signaling pathway and is integral to cell adhesion. Unique in the study of tumor suppressors, β-catenin functions both as a structural component in cell adhesion complexes and as a transcriptional co-activator in the nucleus. Dysregulation of β-catenin is often associated with various cancers, making anti-catenin beta-1 antibodies essential tools for specific detection and quantification. These antibodies contribute significantly to understanding the dual role of β-catenin and its involvement in cancer initiation and progression.

Western blot using anti-E Cadherin antibody 19A11 (Ab03204)

Why Go Recombinant?

Absolute Antibody’s recombinant engineered antibodies, available in diverse formats and species, are essential tools for studying tumor suppressors. They enable specific detection and quantification of protein expression, including isoform-specific analysis. These antibodies facilitate functional studies, allowing manipulation of tumor suppressor activity, and can be utilized in high-throughput screening for drug discovery. Their species compatibility supports research across various organisms, contributing to the understanding of tumor suppressor biology.

Additionally, the recombinant antibodies we offer are 100% biologically defined, opening further opportunities for our antibody engineers to customize these tools to your exact experimental needs. For more information on our custom services, we have resources available on formats we have made (including bi-, tri-, and multispecific formats), our recombinant production platform, and more. If your questions still aren’t answered, reach out! Our team is happy to help with any product questions or orders.

IF staining of HeLa cells using anti-beta catenin 15B8 (Ab01655-23.0)

More Research Products

As an Absolute Biotech brand, Absolute Antibody is proud to partner with our sister companies to provide an even more comprehensive menu of antibody-based research tools. Other reagents include:

• Human BRCA1 Antibody ELISA Kits from LSBio for the quantitative detection of Human BRCA1 in serum. Built for 96-well systems, this sandwich assay can detect BRCA1 down to 0.312 ng/ml.
• Monoclonal Mouse anti‑Human SMAD4 Antibody from LSBio. These antibodies have been validated for IHC applications in frozen and paraffin-embedded tissues.
• Goat Anti-VHL Antibody from Everest Biotech. These polyclonal antibodies detect von Hippel-Lindau (VHL) tumor suppressor protein concentrations with high affinity in peptide ELISA, western blot, and IHC applications.
• Polyclonal Rabbit anti‑Human APC Antibody from LSBio, reactive with human, mouse and rat. This polyclonal antibody has been validated for ELISA, IF, IHC, and western blot.
• Goat Anti-NUMB Antibody from Everest Biotech. This polyclonal antibody has been validated for peptide ELISA, IHC, IF, and flow cytometry.

Tumor Suppressor Antibodies

Human antibodies from patients are being sequenced at an increasing rate – see Quake et al. for an up-to-date review of high-throughput sequencing of antibodies. Amongst others, we are excited to offer you a range of human-derived antibodies specific to viral pathogens or self-antigen (many in collaboration with Humabs BioMed SA, Bellinzona, Switzerland). Our proprietary cloning platform allows us to produce high-quality recombinant versions of these antibodies – we think some will be valuable reagents for research and diagnostics. We offer the original human IgG version as well as reformatted mouse and rabbit versions to suit your experimental requirements- contact us for bulk custom orders.

Adjacent figure shows: Indirect immunofluorescence (IF) staining of live keratinocyte monolayers with pemphigus vulgaris (PV) auto-antibodies
(A) Cryosections of human skin stained with biotinylated DSG3-specific monoclonal antibodies (Ab00153, Ab00155, Ab00158 and Ab00156).
(B) Binding of a panel of pemphigus auto-antibodies to DSG3-coated ELISA plates in the presence or absence of EDTA. Antibodies described in (external links): Corti D et al. 2010 as well as Di Zenzo G et al. 2012.
(C) Staining of human epidermis with biotinylated PVB28 (Ab00158) in the presence (with) or absence (w/o) of Ca2+

Antibodies against proteins involved in co-stimulation and other aspects of immune cell regulation are of particular interest to therapeutics developers. Some have already entered the clinic, with more in the development pipeline. However, many aspects of immune cell signaling are still unknown, and researchers require ever more advanced tools to tap into this potential. Absolute Antibody has developed a collection of recombinant engineered antibodies against clinically relevant proteins, including mouse homologues of current therapeutic targets.

• Your favorite research tools, now updated through antibody engineering for improved performance in vivo
• Reduce immunogenicity by matching antibody species to your host organism
• Tailor effector function by choosing from our range of antibody isotypes and subtypes
• Explore new possibilities by combining two of these antibodies into a custom bispecific reagent

Contact us if you have any questions or would like a quote.

See also: Our Fc fusion proteins are matched to our collection of immunotherapy antibodies, with all products available in a range of species-specific and engineered Fc domains.

Engineered Mouse-Anti-Mouse Antibodies

Our research-grade biosimilars are research tools for the study and evaluation of biological processes without the need to source and purchase costly therapeutic-grade biologics. Free of excipients and/or additives found in the therapeutic formulations, our biosimilars require fewer extra controls in an experimental setting. In addition to the original human format, our biosimilars are available with mouse, rabbit, rhesus monkey and cynomolgus monkey constant domains.

Absolute Antibody research-grade biosimilars can be used as controls:

• To benchmark novel biotherapeutics (biologics) for pre-clinical lead identification.
• For the development of potency assays.

The growing range of research-grade biosimilars available from Absolute Antibody includes Trastuzumab, Muromonab, Campath-1H, Rituximab, Infliximab, Daclizumab, Basiliximab, Cetuximab, Satumomab, Acritumomab, Abaciximab, Eculizumab, Efalizumab, Clenoliximab, Ruplizumab, Matuzumab, Humicade, Volociximab and our most recent addition Oxelumab.

Can’t see what you were looking for? We can custom make any biosimilar of your choice – just send us the sequence. Contact us here.

Product Information

• All research-grade biosimilars are supplied in PBS buffer with preservative (0.02% Proclin 300)
• Endotoxin is < 1.0 EU/mg as determined by the LAL method
• Purity is > 96% as determined by SDS-PAGE
• Our research-grade biosimilars are NOT FOR THERAPEUTIC USE

For further information, to request any antibody in a different format, or for bulk orders please contact us and we’ll be happy to help you.

VivopureX™ antibodies are recombinant mouse antibodies ideal for in vivo research in mouse models. The collection consists of popular antibody clones, many originally obtained from rats or hamsters, which Absolute Antibody has engineered into mouse-anti-mouse recombinant versions to improve research results. These species-matched chimeric antibodies consist of a clone’s original antigen-binding variable domain with a mouse constant domain.

Our VivopureX™ recombinant mouse antibodies offer many advantages compared to the original antibody format, including:

• Reduced immunogenicity: no neutralizing antibodies are induced in the host organism, meaning the antibody works for longer and cohorts respond more consistently.
• Tailored effector function: antibody formats have been engineered to suit their most popular application. (Increased Fc receptor binding increases depleting potential, while reduced Fc receptor binding reduces side effects in blocking.)
• Increased potency: less antibody is required to achieve the same response in mouse models.
• Batch-to-batch reproducibility: recombinant production ensures the highest possible reproducibility, and guarantees high purity and low endotoxins (<0.5 EU/mg).

Mean tumor sizes in animals treated with PD-1 mouse IgG2a Fc Silent™ antibody based on clone RMP1-14 (Ab00813-2.3) are significantly smaller than in animals treated with the traditional rat IgG2a antibody (Ab00813-7.1)

Our data illustrate how VivopureX™ mouse antibodies provide increased long-term efficacy, potency and consistency compared to the original rat antibodies. In the figure to the left, our recombinant mouse PD-1 antibody based on the widely used clone RMP1-14 reduced tumor size in a mouse model more effectively than the original rat version.

Looking for another example? One of our customers used a species-matched mouse-anti-mouse antibody to deplete CD8+ T-cells in mice more completely and for longer than the original rat antibody.

The VivopureX™ antibody collection includes a selection of our most exciting mouse-anti-mouse antibodies, now available at competitively priced bulk prices. All are available in discounted bulk sizes ranging from 1 mg to 100 mg, as well as our standard 200 µg size. In addition, we offer our recombinant isotype controls at up to 50% discount if purchased together with a linked VivopureX™ antibody. Contact us to receive a quote.

We’ll continue to add new antibodies to the list below, so check back for updates! Contact us with questions, to receive a quote, or to ask if another antibody could be made available in a mouse-anti-mouse format in bulk for in vivo research.

Engineered recombinant antibodies for a variety of research areas and applications.

Anti-immunoglobulin antibodies are important reagents used in the detection of antibodies. This may be in the form of a secondary antibody used to detect a primary antibody in methods such as western blot, immunohistochemistry, and immunofluorescence staining for microscopy or flow cytometry. Moreover, anti-immunoglobulin antibodies are important components of diagnostic tests. For example, they may be used in the characterization of immune responses or as control capture lines on lateral flow immunoassays.

Recombinant antibody technology allows you to obtain highly reliable anti-immunoglobulin antibodies tailored to your specific assay. Recombinant production provides the highest possible batch-to-batch reproducibility, while antibody engineering can be used to increase antibody compatibility with other assay components and extend antibody utility beyond the original format. Moreover, all our antibodies are manufactured in a completely animal- and animal-component free production platform.

We are pleased to announce our panel of recombinant anti-human immunoglobulin antibodies. The panel includes antibodies for human IgG, IgM, IgE and IgD, as well as anti-human kappa and lambda light chain antibodies for detection of all human antibodies. Available formats include human, mouse, rabbit and goat backbones, as well as antibody fragments, with other formats available upon request.

The panel is already proving popular among researchers fighting the current coronavirus pandemic, in light of the need for improved serological testing. These antibodies can be used to test for anti-SARS-CoV-2 antibodies in COVID-19 diagnostic tests

View our collection below, and contact us with any questions, to obtain an order quote, or to request pricing for bulk amounts.

Non-human-primate (NHP) models are important in preclinical research and are used as proxies for human immune responses. To help researchers investigate these, we offer a panel of recombinant anti-NHP immunoglobulin antibodies. This panel includes antibodies against key antibody isotypes and subtypes in widely used NHP models, such as rhesus macaque, cynomolgus monkey and baboon.

Anti-immunoglobulin antibodies are important reagents used in the detection of antibodies. Our NHP immunoglobulin antibodies can be used as secondary antibodies to detect primary antibodies in methods such as western blot, immunohistochemistry, and immunofluorescence staining for microscopy or flow cytometry. In addition, anti-immunoglobulin antibodies are important components of assays evaluating immune responses during cancer and autoimmunity drug development, infectious disease studies, and vaccine trials. Most recently, anti-immunoglobulin antibodies are finding extensive application in the study of COVID-19, as well as development of vaccines against SARS-CoV-2.

Recombinant antibody technology allows you to obtain highly reliable anti-immunoglobulin antibodies tailored to your specific assay. Recombinant production provides the highest possible batch-to-batch reproducibility, while antibody engineering can be used to increase antibody compatibility with other assay components and extend antibody utility beyond the original format. Moreover, all our antibodies are manufactured in a completely animal- and animal-component free production platform.

Our recombinant NHP immunoglobulin antibodies are available in formats such as human, mouse, and rabbit backbones, as well as antibody fragments. Other formats are available upon request and further targets will be added regularly – if there is anything specific you’re looking for and can’t find yet, please contact us.

View our NHP immunoglobulin antibodies below. If you are looking for secondary antibodies to human antibodies see here; for any other species, check out our page here. Finally, if you need primary antibodies with a non-human primate backbone, get in touch. Our entire recombinant antibody catalog is available in rhesus macaque and cynomolgus monkey IgG1 and IgG4 isotypes. If there is a particular antibody you cannot find in these formats, please contact us.

The majority of secondary antibodies against antibodies from common research models such as mouse or rat are polyclonal antibodies made in goats or rabbits. These not only suffer from reproducibility issues, but also come with a considerable impact on animal welfare. At Absolute Antibody, all our antibodies, including our secondary antibodies, are manufactured in a recombinant, completely animal- and animal-component free production platform. We are able to offer recombinant anti-immunoglobulin antibodies against mouse or rat antibodies, as well as antibodies that bind rabbit, goat, dog, and elasmobranch (e.g. sharks, rays, skates) antibodies.

Anti-immunoglobulin antibodies are used as secondary antibodies to detect primary antibodies in methods such as western blot, immunohistochemistry, and immunofluorescence staining for microscopy or flow cytometry. Recombinant production provides highly reliable anti-immunoglobulin antibodies with the highest possible batch-to-batch reproducibility. In addition, recombinant antibody technology enables antibody engineering to open up new experimental possibilities.

We also offer secondary antibodies against human or other primate antibodies. If you are looking for another species or target, please contact us.

IgE is an often overlooked antibody isotype, but it plays a critical role in allergic reactions and certain autoimmune diseases. To advance related research and diagnostics, Absolute Antibody has used recombinant antibody technology to develop highly reproducible IgE proteins in a wide range of species. These Fc-only proteins can be used for a variety of applications:

• Assay standards: create reproducible control sera by spiking the IgE into normal sera
• Immunogens: generate antibodies against the immunoglobulin Fc domain
• Engineered into antigen-specific antibodies: make an IgE antibody for your allergy or autoimmunity research

New Species Available

We now offer IgE Fc domain proteins in a variety of species. We started with the common models, human and mouse, and have recently expanded our catalog to include animals such as dog, cat and horse. The new species are ideal for veterinary research and diagnostic assay design.

The IgE Fc domain proteins react with species-specific anti-IgE antibodies, with no cross-reactivity observed (see ELISA data below).

Currently available IgE Fc domain proteins are listed below. We also offer immunoglobin Fc domains in other subtypes, including IgG1, IgG2A, IgA and IgM; you can view our full list of Ig Fc domains here. For more information or to place an order, please contact us.

Interested in using these Fc proteins to create antigen-specific antibodies? Choose a target from our extensive catalog, or provide your own antibody, and we’ll do the rest. Check out our selection of anti-allergen antibodies, and contact us to discuss your project!

Fc fusion proteins (also known as Fc chimeric fusion proteins, Fc-Igs, Ig-based chimeric fusion proteins and Fc-tag proteins) are composed of the Fc domain of IgG genetically linked to a peptide or protein of interest. Fc fusion proteins have become valuable reagents for in vivo and in vitro research.

Examples of Fc-fused binding partners include, but are not limited to, single peptides, ligands activated upon cell-surface receptor binding, signaling molecules, extracellular domains of receptors activated upon dimerization and bait proteins used to identify binding partners in protein microarrays.

One of the most valuable characteristics conferred by the Fc domain in vivo is the dramatic prolonging of the plasma half-life (t_1/2) of the protein of interest, which for biotherapeutic drugs can result in improved therapeutic efficacy, an attribute that has made Fc fusion proteins attractive biotherapeutic agents.

n vitro applications of Fc fusion proteins include, among others, immunohistochemistry (IHC), flow cytometry (FC), protein binding assays and use as microarray baits. In these applications, the Fc domain behaves as a supporting module to which proteins can be attached while retaining their native biological activity. The Fc domain can also improve the in vivo and in vitro solubility and stability of some binding partners.

Absolute Antibody offer a diverse array of recombinant Fc fusion proteins with a range of species-specific and genetically engineered Fc domains such as Fc Silent™.

For further information or for bulk and custom orders, do not hesitate to contact us.

Figure 1. A schematic representation of the structure of four immunoglobulin (Ig) classes or isotypes and their Fc domains. The Ig Fc domain does not bind antigen- see Antibody Isotypes and Subtypes and Antibody Effector Functions for a detailed account on structure and function of antibodies.

We offer a range of isotype- and species-specific Ig Fc domains – please see the table below.

Recombinant Ig Fc domains are recommended as:

• Assay standards or controls (see IgG Fc Fusion Proteins)
• Immunogens to generate antibodies against the immunoglobulin Fc domains and other Ig Fc domain applications

For further information or to request a species or isotype not yet shown, please contact us.

Absolute Antibody’s Multi-Tag control protein (Pr00106) and Multi-Tag control protein 2 (Pr00565) are genetically engineered synthetic fusion proteins composed of commonly used epitope tags fused together using flexible linkers (Figure 1 and 2). These Multi-Tag synthetic fusion proteins can be used as a positive control in western/dot blots and ELISAs.

You can also check out our epitope tags page for our full range of recombinant and chimeric anti-epitope tag monoclonal antibodies. For further information on our Multi-Tag control proteins or any of our products, contact us and we will be happy to help.

Figure 1: A schematic representation of the Absolute Antibody’s Multi-Tag control protein (Pr00106), composed of 17 commonly used epitope tags shown in sequence from N- to C-terminal.

Figure 2: A schematic representation of the Absolute Antibody’s Multi-Tag control protein 2 (Pr00565), composed of 12 commonly used epitope tags shown in sequence from N- to C-terminal.

Table 1: List of tags found in Pr00106 and Pr00565.

Free antibody light chains are important diagnostic markers for a range of disorders including amyloidosis, various myelomas and lymphomas. Absolute levels as well as imbalances in the ratio of free kappa and lambda chains are used as diagnostic and prognostic markers.

The recombinant expression platform used by Absolute Antibody allows the direct expression of free antibody light chains, overcoming the common problems faced in purifying these proteins. Our serum-free culturing system and affinity purification result in ultra-pure product that may be used as calibrators and positive controls for diagnostic assays.

We offer both lambda and kappa light chains.

For larger quantities or more information, please contact us and we will be happy to help you.

Coomassie-stained SDS-PAGE gel of human recombinant free lambda (left) and kappa (right) light chains. Starting from the tissue culture supernatant, a single purification step was perfomed to enrich for recombinant free light chains, both as monomer and dimer (at around 28 and 50 kDa, respectively).