The Blu10 Plus Prestained Protein Ladder is a combination of 10 pre-stained proteins with molecular weights from 6.5 to 270 kDa. The 10 recombinant proteins are covalently coupled with blue chromophore, while 2 orange bands at 30 kDa and 270 kDa and a green band at 52 kDa serve as reference bands. The Blu10 Plus Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

-Guide for Molecular Weight Estimation (kDa)

Migration patterns of Blu10 Plus Prestained Protein Ladder in different electrophoresis conditions are listed below :

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

Nowadays, more and more researchers are studying the high molecular weight proteins, meaning they may need high-resolution prestained protein standards, especially with high molecular weights. That’s why we’re pleased to launch the color-enhanced Blu10 Plus prestained protein standards.

As you may see the comparison data shown below, Blu10 Plus shows a better resolution than the Blu12 & Blu10 for the protein bands from 95 kDa to 270 kDa

Lane 1: PMU12-0500 UNveil Unstained Protein Ladder (Bio-Helix) / 5μl
Lane 2: PMB01-0500 Blu10 Plus Prestained Protein Ladder (Bio-Helix) / 5μl
Lane 3: Bio-Rad 161-0374 Precision Plus Protein™ Dual Color Standards / 5μl
Lane 4: Thermo Scientific 26616 PageRuler Prestained Protein Ladder / 5μl
Lane 5: PMB12-0500 Blu12 Prestained Protein Ladder (Bio-Helix) / 5μl
Lane 6: PMU12-0500 UNveil Unstained Protein Ladder (Bio-Helix) / 10μl
Lane 7: PMB01-0500 Blu10 Plus Prestained Protein Ladder (Bio-Helix) / 10μl
Lane 8: Bio-Rad 161-0374 Precision Plus Protein™ Dual Color Standards / 10μl
Lane 9: Thermo Scientific 26616 PageRuler Prestained Protein Ladder / 10μl
Lane 10: PMB12-0500 Blu12 Prestained Protein Ladder (Bio-Helix) / 10μl

Required materials but not provided:

Vertical Electrophoresis system
Power supplies
Vortex or equivalent
Microcentrifuge

► One-Vial-Fits-All: New broad-range protein ladder from 6.5kDa to 270kDa.
► Higher Added Value: Tri-colors and 10 bands with premium quality.
► Better performance for higher molecular weight proteins.
► Clear separation map from 6.5 kDa to 270 kDa in the Tris-Glycine system.
► Enhanced transfer, wash, and stripping durabilities.

Storage Buffer :
62.5 mM Tris-H3PO4 (pH 7.5 at 25 °C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol.

Storage:
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.

Lane 1: PMB11-0500 (Bio-Helix) 5ul
Lane 2: PMB13-0500 (Bio-Helix) 5ul
Lane 3: PMB12-0500 (Bio-Helix) 5ul
Lane 4: PMB10-0500 (Bio-Helix) 5ul
Lane 5: PMB01-0500 (Bio-Helix) 5ul
Lane 6: PMB11-0500 (Bio-Helix) 5ul
Lane 7: PMB01-0500 (Bio-Helix) 5ul
Lane 8: PMU12-0500 (Bio-Helix) 5ul
Lane 9: thermo Mark12 5ul
Lane 10: Bio-Rad 161-0317 5ul

*NuPAGE Bis-Tris 4-12% was used to run SDS-PAGE in MOPS and MES buffer. 5ul of protein ladder samples was loaded into each lane.

The IRIS11 Prestained Protein Ladder is a combination of 11 pre-stained proteins with molecular weights from 3 to 260 kDa. The 11 recombinant proteins are covalently coupled with blue chromophore, while 2 red bands at 70 kDa and 260 kDa, a green band at 15 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. The IRIS11 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.

Guide for Molecular Weight Estimation (kDa)

Refer to the IRIS11 Prestained Protein Ladder patterns in various electrophoresis conditions:

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.

Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.

Storage Buffer :

0.2~0.4 mg/ml of each band of protein is stored in a mixture of solution that contains: 20 mM Trisphosphate at pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.

Quality Control :
5μl of IRIS11 Prestained Protein Ladder (PMI11) resolves 11 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.

Storage :
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.

Vertical Electrophoresis system
Power supplies
Vortex or equivalent
Microcentrifuge

COOMASSIE nano ╴The Next Generation Protein Staining Solution

COOMASSIE nano, enhanced by nano-technology, is a ready-to-use protein staining solution for SDS-PAGE gels. Its next generation formula offers a faster protein detection, higher sensitivity and there is no need for destaining. Also, the washing step can be omitted. In the absence of hazardous substances such as methanol and acetic acid, COOMASSIE nano is considered to be safe and environmentally friendly. COOMASSIE nano is also compatible with mass spectrophotometry.

Figure 1. COOMASSIEnano Protein Staining Solution practices the fast-signal representing at 5 min. Prestained protein ladder, unstain protein ladder, and BSA were prepared and applied in electrophoresis. After running SDS-PAGE (4-20% Novex Gel), please remove the gel from the cassette then proceed to submerge the gel in proper amount of COOMASSIEnano dye, enough to cover the gel. Lightly agitate the staining box for 5 minutes to over night at room temperature.

Figure 2. COOMASSIEnano Protein Staining Solution demonstrates the high sensitivity detection could be down to 10 ng (Lane H). Prestained protein ladder, unstained protein ladder, and serial diluted BSA were prepared and applied in electrophoresis. After running 10% homemade gel (0.75 mm thickness), please remove the gel from the cassette then proceed to submerge the gel in a proper amount of COOMASSIEnano Protein Staining Solution, enough to cover the gel. Lightly agitate the staining container at room temperature when staining for 30 hrs.

Figure 3. COOMASSIEnano Protein Staining Solution presents the equivalent or even superior performance on fast signals and clear background compared with other brands. Lightly agitate the staining box for 5 to 30 minutes at room temperature.

Ponceaus S Staining Solution is a ready-to-use membrane stain for evaluating the transfer efficiency of a western blot. This stain is recommended for rapid and reversible protein staining on nitrocellulose or PVDF membranes. Ponceau S staining is reversible and can be removed with a short incubation in 0.1% NaOH.

Figure 1 Ponceau S solution can be used to evaluate for total protein amount or transfer efficiency on nitrocellulose and PVDF membrane. The PVDF membrane stained Ponceau S for 5 mins and washed with ddH2O for 3 mins. It provides visible pink bands.

Lane 1, 13: Prestained Protein Ladder #PM019-0500

Lane 2-7: 2X Dilutions of Unstained Protein Ladder #PMU12-0500

Lane 8-12: 2000, 1000, 500, 100, 50 ng of BSA

Container: box for gel staining
Shaker: orbital or rocking shaker

1. After the SDS-PAGE Gel has been transferred to a nitrocellulose or PVDF membrane, place the transferred membrane in a staining container.
2. Submerge the membrane in a proper amount of Ponceau S Protein Staining Solution that is enough to cover the whole membrane. Lightly agitate the staining container for 5 to 15 minutes at room temperature. Protein bands will be stained and start becoming visible in 5 to 15 minutes.
3. Wash away the excessive stain on the membrane with DI water until the protein bands are clear.
4. For the further membrane blocking step, remove the Ponceau S Staining Solution from the membrane by placing the membrane in 20 ml 0.1% NaOH for 1 to 2 minutes then rinse in DI water for 1 to 2 minutes.

Important notes

The Ponceau S Protein Staining Solution is provided as a ready-to-use solution, there is no need to dilute before use.

The UltraScence Pico Plus Western Substrate, as a luminol-based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The low picogram or high femtogram detection of antigen is enabled by UltraScence Pico Plus Western Substrate’s excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.

Product Name: UltraScence Pico Plus Western Substrate

Product Item No.: CCH321-B100ML

Dimensions: 100ML

Substrate Type: HRP (Horseradish Peroxidase) Substrate

Contents/Storage: Store at 4℃ for 2 years

Patent No.: US10,711,185

– No optimization required.
Switching to the UltraScence Pico Plus Western Substrate from other brands, such as Thermo Scientific™ SuperSignal™ Pico Plus and Bio-Rad™ Clarity™, does not require optimization or protocol changes.

– High degree of sensitivity and enhanced chemiluminescence duration.
UltraScence Pico Plus Western Substrate enables an accurate low picogram or high femtogram detection of protein on the same immunoblot after a single exposure.

– Optimized for use with PVDF and nitrocellulose membranes.

– Compatible with Western Blotting Markers.

– Optimized for film and CCD-based imaging.

**US Patent No.: US10,711,185

1. 1. Keep the membrane moist in the wash buffer while preparing the substrate mixture. Make sure the membrane does not dry out in the next steps.2. The chemiluminescent substrate solution is sufficiently stirred to prepare the 0.1ml of solution / cm2 of the membrane.- For a small membrane (7 x 8.5 cm), 4 ml of solution is sufficient.
   – For a medium-sized membrane (8.5 x 13.5 cm), 10 ml of solution is sufficient.3. Place the membrane on a clean, flat surface or in a clean container.4. Remove the membrane from the chemiluminescent substrate solution and drain the excess solution.5. Place the membrane in a plastic sheet protector or plastic wrap to prevent the film from drying out.6. Imaging the membrane with a digital imager or by exposure to the X-ray film.

Figure 1. UltraScence Pico Plus Western Substrate enables an accurate low picogram to high femtogram detection of protein on the same immunoblot after a single exposure. Membranes were probed with GFP tag Rabbit PolyAb diluted at 1:10,000 of and then with Goat Anti-rabbit IgG/HRP secondary antibody (1:10,000) after serial dilution EGFP (Enhanced Green Fluorescent Protein) were prepared and applied in electrophoresis and protein transfer. Identical blots were incubated with the Western substrate. The blots were simultaneously exposed for 5 seconds, 10 seconds, 30 seconds, 60 seconds, and 180 seconds using ChemluxSPX-600 Series digital imaging system.

* SuperSignal West Pico PlUS and Clarity Western ECL Substrate is a registered trademark of Thermo Fisher Scientific and Bio rad. The trademark holder is not affiliated with Bio-HeliX Co., Ltd. and does not recognize this product.

Q: From Bio-Helix’s UltraScence ECL substrates portfolio, which level of ECL fits best my Western blot applications?
A: UltraScence ECL substrates series is compatible with the use from low picogram to low-femtogram level detections. Please kindly refer to the ECL selection guide of UltraScence Western substrate as the below table:

(a) CCH321-B100ML, UltraScence Pico Plus Western Substrate
(b) CCH345-B100ML, UltraScence Pico Ultra Western Substrate
(c) CCH365-B100ML, UltraScence Femto ECL Western Substrate

(d) CCH375-B100ML, UltraScence Femto Plus ECL Western Substrate

(e) CCH385-B100ML, UltraScence Atto Western Substrate

The UltraScence Pico Ultra Western Substrate, as a luminol-based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The low picogram to mid femtogram detection of antigen is enabled by UltraScence Pico Ultra Western Substrate’s excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.

Product Name: UltraScence Pico Ultra Western Substrate

Product Item No.: CCH345-B100ML

Dimensions: 100ML

Substrate Type: HRP (Horseradish Peroxidase) Substrate

Contents/Storage: Store at 4℃ for 2 years

**US Patent No.: US10,711,185

– No optimization required.
Switching to theUltraScence Pico Ultra Western Substrate from other brands, such as Thermo Scientific™ SuperSignal™ West Dura and Millipore™ Immobilon™ substrates, does not require optimization or protocol changes.

– High degree of sensitivity and enhanced chemiluminescence duration.
UltraScence Pico Ultra Western Substrate enables an accurate low picogram to mid femtogram detection of protein on the same immunoblot after a single exposure.

– Optimized for use with PVDF and nitrocellulose membranes.
– Compatible with Western Blotting Markers.
– Optimized for film- and CCD-based imaging.
– Patent pending product.

**US Patent No.: US10,711,185

1. Keep the membrane moist in the wash buffer while preparing the substrate mixture. Make sure the membrane does not dry out in the next steps.

2. The chemiluminescent substrate solution is sufficiently stirred to prepare the 0.1ml of solution / cm2 of the membrane.

– For a small membrane (7 x 8.5 cm), 4 ml of solution is sufficient.
– For a medium-sized membrane (8.5 x 13.5 cm), 10 ml of solution is sufficient.

3. Place the membrane on a clean, flat surface or in a clean container.

4. Remove the membrane from the chemiluminescent substrate solution and drain the excess solution.

5. Place the membrane in a plastic sheet protector or plastic wrap to prevent the film from drying out.

6. Imaging the membrane with a digital imager or by exposure to the X-ray film.

Figure 1. UltraScence Pico Ultra Western Substrate enables an accurate low picogram to mid femtogram detection of protein on the same immunoblot after a single exposure. Membranes were probed with GFP tag Rabbit PolyAb diluted at 1:10,000 of and then with Goat Anti-rabbit IgG/HRP secondary antibody (1:10,000) after serial dilution EGFP (Enhanced Green Fluorescent Protein) were prepared and applied in electrophoresis and protein transfer. Identical blots were incubated with the Western substrate. The blots were simultaneously exposed for 5 seconds, 10 seconds, 30 seconds, 60 seconds, and 180 seconds using ChemluxSPX-600 Series digital imaging system.

*Immobilon™ Western Chemiluminescent HRP Substrate is a registered trademark of Millipore, Amersham ECL™ Prime Western Blotting Detection Reagent is a registered trademark of Cytiva, and SuperSignal West Dura is a registered trademark of Thermo Fisher Scientific. The trademark holder is not affiliated with Bio-HeliX Co., Ltd. and does not endorse these products.

Q: From Bio-Helix’s UltraScence ECL substrates portfolio, which level of ECL fits best my Western blot applications?
A: UltraScence ECL substrates series is compatible with the use from low picogram to low-femtogram level detections. Please kindly refer to the ECL selection guide of UltraScence Western substrate as the below table:

(a) CCH321-B100ML, UltraScence Pico Plus Western Substrate
(b) CCH345-B100ML, UltraScence Pico Ultra Western Substrate
(c) CCH365-B100ML, UltraScence Femto ECL Western Substrate

(d) CCH375-B100ML, UltraScence Femto Plus ECL Western Substrate

(e) CCH385-B100ML, UltraScence Atto Western Substrate

Product Name: UltraScence Femto Plus Western Substrate

Product Item No.: CCH375-B100ML

Size: 100ml

Substrate Type: HRP (Horseradish Peroxidase) Substrate

Contents/Storage: Store at 4℃ for 2 years

US Patent No.: US10,711,185

– No optimization required.
Switching to the UltraScence Femto Plus Western Substrate from other brands, such as Thermo Scientific™ SuperSignal™ West Femto, does not require optimization or protocol changes.

– High degree of sensitivity and enhanced chemiluminescence duration.
UltraScence Femto Plus Western Substrate enables an accurate mid femtogram to low femtogram detection of protein on the same immunoblot after a single exposure.

– Optimized for use with PVDF and nitrocellulose membranes.

– Compatible with Western Blotting Markers.

– Optimized for film and CCD-based imaging.

Membranes were probed with GFP tag Rabbit PolyAb diluted at 1:10,000 of and then with Goat Anti-rabbit IgG/HRP secondary antibody (1:10,000) after serial dilution EGFP (Enhanced Green Fluorescent Protein) were prepared and applied in electrophoresis and protein transfer. Identical blots were incubated with 400 μL of UltraScence Femto Plus Western substrate (CCH375). The blots were simultaneously exposed for 5seconds, 10 seconds, 30 seconds, 60 seconds and 180 seconds and using Chemlux SPX-600 Series digital imaging system.

The membrane images show that UltraScence Femto Plus Western Substrate provide sensitivity the same as Thermo ScientificTM SuperSignalTM West Femto. They can both clearly detect 5 ng EGFP after exposure for 180 sec. Membranes were probed with GFP tag Rabbit PolyAb diluted (1:10,000) and then with Goat Anti-rabbit IgG/HRP secondary antibody (1:10,000) after serial dilution EGFP (Enhanced Green Fluorescent Protein) were prepared and applied in electrophoresis and protein transfer. Identical blots were incubated with 400 μL of UltraScence Femto Plus Western substrate (CCH375-B100ML). The blots were simultaneously exposed for 5, 10, 30, 60 and 180 seconds and using Chemlux SPX-600 Series digital imaging system.

* SuperSignal West Femto is a registered trademark of Thermo Fisher Scientific. The trademark holder is not affiliated with Bio-HeliX Co., Ltd. and does not recognize this product.

Q: From Bio-Helix’s UltraScence ECL substrates portfolio, which level of ECL fits best my Western blot applications?
A: UltraScence ECL substrates series is compatible with the use from low picogram to low-femtogram level detections. Please kindly refer to the ECL selection guide of UltraScence Western substrate as the below table:

(a) CCH321-B100ML, UltraScence Pico Plus Western Substrate
(b) CCH345-B100ML, UltraScence Pico Ultra Western Substrate
(c) CCH365-B100ML, UltraScence Femto ECL Western Substrate

(d) CCH375-B100ML, UltraScence Femto Plus ECL Western Substrate

(e) CCH385-B100ML, UltraScence Atto ECL Western Substrat

A novel way to perform Western Blot

Mix OneStep Blocker, 1°Ab and 2° Ab with membrane together, that’s all.

OneStep Blocker is a blocking solution for Western blot analysis.

This OneStep Blocker buffer not only provides blocking, primary and secondary antibody hybridization in one step but also enhances the signal developed with HRP (horseradish peroxidase) or AP (alkaline phosphatase) substrates. It, therefore, serves as both blocker and enhancer in Western analysis. With the 3-in-1 step procedure, OneStep Blocker is a time and labor economic solution for the time consuming and laborious Western procedure.

SAVE & REUSE YOUR ANTIBODIES

One-Step Blocker can also be generally applied as a TWO-STEP BLOCKER.

Features of OneStep Blocker – Western blocking solution and a signal enhancer (Protein free)

• 3 steps in one: Block the membrane and dilute 1o Ab & 2o Ab in one step.
• Enhance antibody signal: It shows a two- to five-fold increase in signal intensity for most protein targets, enabling much less protein to be detected with the same substrate and method.
• Enhance time-saving: It saves at least 2 hours in the antibody detection process during the Western Blot, with only one hour needed.
• Universal antibody diluent: Ready-to-use dilution buffer for most of 1o Ab & 2o Ab.
• No blocking step needed: Just immerse the membrane in the OneStep Blocker solution with your antibodies. That’s all.
• Effective with any ECL: After the antibody detection process, the signal can be developed with both HRP (horseradish peroxidase) and AP (alkaline phosphatase) substrates.
• Less hands-on steps: No 3 wash steps are required, meaning no need to transfer the membrane in&out of the container.
• Compatible with PVDF & NC membrane: Regardless of the pore size, the OneStep Blocker minimizes the background from non-specific protein binding by antibodies.
• Improve protein detection: Improve the binding process of target proteins, so that specific antibodies can bind more effectively.
• Protein free: Reduces overall background and minimizes non-specific signals often seen with ECL detection.

** Required materials but not provided

＃Primary antibody.
＃Secondary antibody conjugated with HRP.
＃Wash buffer:

PBST (phosphate buffered saline with Tween-20) or TBST (Tris buffered saline with Tween-20) buffer.
ECL (Enhanced chemiluminescence) or colorimetric reagents.

＃Shaker: orbital or rocking shaker.

1. After Western blot transferring, immerse the PVDF or NC membrane in PBST buffer for 5 minutes.

2. Dilute the primary antibody and secondary antibody with proper amounts of OneStep Blocker.

• 2.1 For example, when the dilution factor for both primary and secondary antibodies is 1: 10,000, add 2μl of the primary antibody to  10ml of  the OneStep Blocker (1st tube), followed by adding 2μl of the secondary antibody to another 10ml of the OneStep Blocker (2nd tube).
• 2.2 Thoroughly mix the antibody-OneStep Blocker solution inside each tube by inverting it back and forth.
• 2.3 Pour the primary antibody-OneStep Blocker solution into the prepared container first, followed by the addition of the secondary antibody-OneStep Blocker solution into the same container.

3. Incubate the membrane immediately in the antibody-OneStep Blocker solution at room temperature for 1 – 2 hours with gentle agitation. Please note that after mixing  the primary and secondary antibodies, the membrane needs to be immediately immersed in the mixture within 10 minutes for obtaining the optimal performance.

4. Wash the membrane with PBST/TBST three times with shaking.

5. Drain excessive wash buffer and perform image development methods with ECL or colorimetric system immediately.

Can Primary Antibody & Secondary Antibody be hybridized in 1 step with great efficiency?

1. Please note that after mixing the primary and secondary antibodies, the membrane needs to be immediately immersed in the mixture within 10 minutes for obtaining the optimal performance.

2. The dilution for the secondary antibody should be at least 1:10,000 or more. A higher level of background noise will be observed as a result with a high concentration of secondary antibody.

3. Do not incubate membrane in OneStep Blocker for over 4 hours to avoid high background. Overnightincubation is especially not recommended.

4. The primary/secondary antibodies mixed in OneStep Blocker solution may be reused within 3 days. Enhancing effect may trail off along with the increasing storage time or repetitiveness. Keep the mixed solution refrigerated. For critical experiment or strong signal, fresh preparation of antibody-OneStep Blocker solution is required.

5. When the antibody concentration is too high or if the prolonged incubation takes place, it will cause high background. When excessive background occurs, please try the followings:
(a) Reduce/optimize primary and/or secondary antibody concentrations.
(b) Use dot-blot test to optimize antibody concentrations.
(c) Reduce/optimize incubation time.

Description

Nitrocellulose/Filter Paper Sandwiches, 0.2 μm, as high-quality membranes ideal for blotting proteins and nucleic acids, are ideal for the transfer of low molecular weight proteins (less than 20 kDa) and nucleic acids (less than 300 bp), exhibiting high sensitivity and low background for immunoblotting.

Specifications

Dimensions/Size: 7.3 cm x 8.3 cm

Material: Nitrocellulose membrane

Wettability: Hydrophilic

Thickness: 110-120 μm

Pore Size: 0.2 μm

BSA Protein Binding Capacity: ~200 μg/cm²

Description

Nitrocellulose/Filter Paper Sandwiches, 0.2 μm, as high-quality membranes ideal for blotting proteins and nucleic acids, are ideal for the transfer of low molecular weight proteins (less than 20 kDa) and nucleic acids (less than 300 bp), exhibiting high sensitivity and low background for immunoblotting.

Specifications

Dimensions/Size: 7.3 cm x 8.3 cm

Material: Nitrocellulose membrane

Wettability: Hydrophilic

Thickness: 110-120 μm

Pore Size: 0.2 μm

BSA Protein Binding Capacity: ~200 μg/cm²

Description

For analyzing the small amounts of proteins (down to 10 pmoles), peptides or amino acids, polyvinylidene difluoride (PVDF) membranes are the most ideal item for tracing down these transferred small molecular weight materials after electroblotting. The UltraScence PVDF/Filter Paper Sandwiches, 0.2 μm, have excellent binding properties for western blotting, dot-blot assays, and other protein or nucleic acid methods such as protein sequencing

Specifications

Dimensions/Size: 7.3 cm x 8.3 cm

Material: PVDF membrane

Wettability: Hydrophobic

Thickness: 140~150μm

Pore Size: 0.2 μm

BSA Protein Binding Capacity: ~300 μg/cm²

Description

For analyzing the small amounts of proteins (down to 10 pmoles), peptides or amino acids, polyvinylidene difluoride (PVDF) membranes are the most ideal item for tracing down these transferred small molecular weight materials after electroblotting. The UltraScence PVDF/Filter Paper Sandwiches, 0.2 μm, have excellent binding properties for western blotting, dot-blot assays, and other protein or nucleic acid methods such as protein sequencing

Specifications

Dimensions/Size: 8.5 cm x 14 cm

Material: PVDF membrane

Wettability: Hydrophobic

Thickness: 140~150 μm

Pore Size: 0.2 μm

BSA Protein Binding Capacity: ~300 μg/cm²