COMING SOON!
Bio-Helix has been focusing on providing the best bio-reagents for life science researchers all over the world since 2007. Working together with scientists in the lab around the world and continuing efforts of quality improvement, we begin to gain trusts and appraises from customers. The brand, spreading in more than forty countries, has become a trustworthy and welcome brand of reagents in laboratories of life science.
The Blu10 Plus Prestained Protein Ladder is a combination of 10 pre-stained proteins with molecular weights from 6.5 to 270 kDa. The 10 recombinant proteins are covalently coupled with blue chromophore, while 2 orange bands at 30 kDa and 270 kDa and a green band at 52 kDa serve as reference bands. The Blu10 Plus Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
Migration patterns of Blu10 Plus Prestained Protein Ladder in different electrophoresis conditions are listed below :
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.
Nowadays, more and more researchers are studying the high molecular weight proteins, meaning they may need high-resolution prestained protein standards, especially with high molecular weights. That’s why we’re pleased to launch the color-enhanced Blu10 Plus prestained protein standards.
As you may see the comparison data shown below, Blu10 Plus shows a better resolution than the Blu12 & Blu10 for the protein bands from 95 kDa to 270 kDa
Lane 1: PMU12-0500 UNveil Unstained Protein Ladder (Bio-Helix) / 5μl
Lane 2: PMB01-0500 Blu10 Plus Prestained Protein Ladder (Bio-Helix) / 5μl
Lane 3: Bio-Rad 161-0374 Precision Plus Protein™ Dual Color Standards / 5μl
Lane 4: Thermo Scientific 26616 PageRuler Prestained Protein Ladder / 5μl
Lane 5: PMB12-0500 Blu12 Prestained Protein Ladder (Bio-Helix) / 5μl
Lane 6: PMU12-0500 UNveil Unstained Protein Ladder (Bio-Helix) / 10μl
Lane 7: PMB01-0500 Blu10 Plus Prestained Protein Ladder (Bio-Helix) / 10μl
Lane 8: Bio-Rad 161-0374 Precision Plus Protein™ Dual Color Standards / 10μl
Lane 9: Thermo Scientific 26616 PageRuler Prestained Protein Ladder / 10μl
Lane 10: PMB12-0500 Blu12 Prestained Protein Ladder (Bio-Helix) / 10μl
Vertical Electrophoresis system
Power supplies
Vortex or equivalent
Microcentrifuge
► One-Vial-Fits-All: New broad-range protein ladder from 6.5kDa to 270kDa.
► Higher Added Value: Tri-colors and 10 bands with premium quality.
► Better performance for higher molecular weight proteins.
► Clear separation map from 6.5 kDa to 270 kDa in the Tris-Glycine system.
► Enhanced transfer, wash, and stripping durabilities.
Storage Buffer :
62.5 mM Tris-H3PO4 (pH 7.5 at 25 °C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol.
Storage:
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.
Lane 1: PMB11-0500 (Bio-Helix) 5ul
Lane 2: PMB13-0500 (Bio-Helix) 5ul
Lane 3: PMB12-0500 (Bio-Helix) 5ul
Lane 4: PMB10-0500 (Bio-Helix) 5ul
Lane 5: PMB01-0500 (Bio-Helix) 5ul
Lane 6: PMB11-0500 (Bio-Helix) 5ul
Lane 7: PMB01-0500 (Bio-Helix) 5ul
Lane 8: PMU12-0500 (Bio-Helix) 5ul
Lane 9: thermo Mark12 5ul
Lane 10: Bio-Rad 161-0317 5ul
*NuPAGE Bis-Tris 4-12% was used to run SDS-PAGE in MOPS and MES buffer. 5ul of protein ladder samples was loaded into each lane.
The IRIS11 Prestained Protein Ladder is a combination of 11 pre-stained proteins with molecular weights from 3 to 260 kDa. The 11 recombinant proteins are covalently coupled with blue chromophore, while 2 red bands at 70 kDa and 260 kDa, a green band at 15 kDa and a newly designed peacock green band at 60 kDa serve as reference bands. The IRIS11 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating the target protein size and validating the Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes.
Refer to the IRIS11 Prestained Protein Ladder patterns in various electrophoresis conditions:
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring.
Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment.
Storage Buffer :
0.2~0.4 mg/ml of each band of protein is stored in a mixture of solution that contains: 20 mM Trisphosphate at pH 7.5, 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol.
Quality Control :
5μl of IRIS11 Prestained Protein Ladder (PMI11) resolves 11 bands in 4-20% SDS-PAGE (Tris-glycine buffer) and after Western blotting to PVDF membrane.
Storage :
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
Stable for up to 24 months at -20°C.
Vertical Electrophoresis system
Power supplies
Vortex or equivalent
Microcentrifuge
COOMASSIE nano, enhanced by nano-technology, is a ready-to-use protein staining solution for SDS-PAGE gels. Its next generation formula offers a faster protein detection, higher sensitivity and there is no need for destaining. Also, the washing step can be omitted. In the absence of hazardous substances such as methanol and acetic acid, COOMASSIE nano is considered to be safe and environmentally friendly. COOMASSIE nano is also compatible with mass spectrophotometry.
Figure 1. COOMASSIEnano Protein Staining Solution practices the fast-signal representing at 5 min. Prestained protein ladder, unstain protein ladder, and BSA were prepared and applied in electrophoresis. After running SDS-PAGE (4-20% Novex Gel), please remove the gel from the cassette then proceed to submerge the gel in proper amount of COOMASSIEnano dye, enough to cover the gel. Lightly agitate the staining box for 5 minutes to over night at room temperature.
Figure 2. COOMASSIEnano Protein Staining Solution demonstrates the high sensitivity detection could be down to 10 ng (Lane H). Prestained protein ladder, unstained protein ladder, and serial diluted BSA were prepared and applied in electrophoresis. After running 10% homemade gel (0.75 mm thickness), please remove the gel from the cassette then proceed to submerge the gel in a proper amount of COOMASSIEnano Protein Staining Solution, enough to cover the gel. Lightly agitate the staining container at room temperature when staining for 30 hrs.
Figure 3. COOMASSIEnano Protein Staining Solution presents the equivalent or even superior performance on fast signals and clear background compared with other brands. Lightly agitate the staining box for 5 to 30 minutes at room temperature.
Ponceaus S Staining Solution is a ready-to-use membrane stain for evaluating the transfer efficiency of a western blot. This stain is recommended for rapid and reversible protein staining on nitrocellulose or PVDF membranes. Ponceau S staining is reversible and can be removed with a short incubation in 0.1% NaOH.
Figure 1 Ponceau S solution can be used to evaluate for total protein amount or transfer efficiency on nitrocellulose and PVDF membrane. The PVDF membrane stained Ponceau S for 5 mins and washed with ddH2O for 3 mins. It provides visible pink bands.
Lane 1, 13: Prestained Protein Ladder #PM019-0500
Lane 2-7: 2X Dilutions of Unstained Protein Ladder #PMU12-0500
Lane 8-12: 2000, 1000, 500, 100, 50 ng of BSA
Container: box for gel staining
Shaker: orbital or rocking shaker
The Ponceau S Protein Staining Solution is provided as a ready-to-use solution, there is no need to dilute before use.
The UltraScence Pico Plus Western Substrate, as a luminol-based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The low picogram or high femtogram detection of antigen is enabled by UltraScence Pico Plus Western Substrate’s excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.
Product Name: UltraScence Pico Plus Western Substrate
Product Item No.: CCH321-B100ML
Dimensions: 100ML
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Contents/Storage: Store at 4℃ for 2 years
Patent No.: US10,711,185
– No optimization required.
Switching to the UltraScence Pico Plus Western Substrate from other brands, such as Thermo Scientific™ SuperSignal™ Pico Plus and Bio-Rad™ Clarity™, does not require optimization or protocol changes.
– High degree of sensitivity and enhanced chemiluminescence duration.
UltraScence Pico Plus Western Substrate enables an accurate low picogram or high femtogram detection of protein on the same immunoblot after a single exposure.
– Optimized for use with PVDF and nitrocellulose membranes.
– Compatible with Western Blotting Markers.
– Optimized for film and CCD-based imaging.
**US Patent No.: US10,711,185
Figure 1. UltraScence Pico Plus Western Substrate enables an accurate low picogram to high femtogram detection of protein on the same immunoblot after a single exposure. Membranes were probed with GFP tag Rabbit PolyAb diluted at 1:10,000 of and then with Goat Anti-rabbit IgG/HRP secondary antibody (1:10,000) after serial dilution EGFP (Enhanced Green Fluorescent Protein) were prepared and applied in electrophoresis and protein transfer. Identical blots were incubated with the Western substrate. The blots were simultaneously exposed for 5 seconds, 10 seconds, 30 seconds, 60 seconds, and 180 seconds using ChemluxSPX-600 Series digital imaging system.
* SuperSignal West Pico PlUS and Clarity Western ECL Substrate is a registered trademark of Thermo Fisher Scientific and Bio rad. The trademark holder is not affiliated with Bio-HeliX Co., Ltd. and does not recognize this product.
Q: From Bio-Helix’s UltraScence ECL substrates portfolio, which level of ECL fits best my Western blot applications?
A: UltraScence ECL substrates series is compatible with the use from low picogram to low-femtogram level detections. Please kindly refer to the ECL selection guide of UltraScence Western substrate as the below table:
(a) CCH321-B100ML, UltraScence Pico Plus Western Substrate
(b) CCH345-B100ML, UltraScence Pico Ultra Western Substrate
(c) CCH365-B100ML, UltraScence Femto ECL Western Substrate
(d) CCH375-B100ML, UltraScence Femto Plus ECL Western Substrate
(e) CCH385-B100ML, UltraScence Atto Western Substrate
The UltraScence Femto Plus Western Substrate, as a luminol-based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The UltraScence Femto Plus Western Substrate is designed for the detection of the target proteins in amounts that are too small to be seen with typical ECL substrates. The low femtogram detection (mid-atto) of antigen is enabled by UltraScence Femto Plus Western Substrate’s excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.
Product Name: UltraScence Femto Plus Western Substrate
Product Item No.: CCH375-B100ML
Size: 100ml
Substrate Type: HRP (Horseradish Peroxidase) Substrate
Contents/Storage: Store at 4℃ for 2 years
US Patent No.: US10,711,185
– No optimization required.
Switching to the UltraScence Femto Plus Western Substrate from other brands, such as Thermo Scientific™ SuperSignal™ West Femto, does not require optimization or protocol changes.
– High degree of sensitivity and enhanced chemiluminescence duration.
UltraScence Femto Plus Western Substrate enables an accurate mid femtogram to low femtogram detection of protein on the same immunoblot after a single exposure.
– Optimized for use with PVDF and nitrocellulose membranes.
– Compatible with Western Blotting Markers.
– Optimized for film and CCD-based imaging.
Membranes were probed with GFP tag Rabbit PolyAb diluted at 1:10,000 of and then with Goat Anti-rabbit IgG/HRP secondary antibody (1:10,000) after serial dilution EGFP (Enhanced Green Fluorescent Protein) were prepared and applied in electrophoresis and protein transfer. Identical blots were incubated with 400 μL of UltraScence Femto Plus Western substrate (CCH375). The blots were simultaneously exposed for 5seconds, 10 seconds, 30 seconds, 60 seconds and 180 seconds and using Chemlux SPX-600 Series digital imaging system.
The membrane images show that UltraScence Femto Plus Western Substrate provide sensitivity the same as Thermo ScientificTM SuperSignalTM West Femto. They can both clearly detect 5 ng EGFP after exposure for 180 sec. Membranes were probed with GFP tag Rabbit PolyAb diluted (1:10,000) and then with Goat Anti-rabbit IgG/HRP secondary antibody (1:10,000) after serial dilution EGFP (Enhanced Green Fluorescent Protein) were prepared and applied in electrophoresis and protein transfer. Identical blots were incubated with 400 μL of UltraScence Femto Plus Western substrate (CCH375-B100ML). The blots were simultaneously exposed for 5, 10, 30, 60 and 180 seconds and using Chemlux SPX-600 Series digital imaging system.
* SuperSignal West Femto is a registered trademark of Thermo Fisher Scientific. The trademark holder is not affiliated with Bio-HeliX Co., Ltd. and does not recognize this product.
Q: From Bio-Helix’s UltraScence ECL substrates portfolio, which level of ECL fits best my Western blot applications?
A: UltraScence ECL substrates series is compatible with the use from low picogram to low-femtogram level detections. Please kindly refer to the ECL selection guide of UltraScence Western substrate as the below table:
(a) CCH321-B100ML, UltraScence Pico Plus Western Substrate
(b) CCH345-B100ML, UltraScence Pico Ultra Western Substrate
(c) CCH365-B100ML, UltraScence Femto ECL Western Substrate
(d) CCH375-B100ML, UltraScence Femto Plus ECL Western Substrate
(e) CCH385-B100ML, UltraScence Atto ECL Western Substrat
