Cytodiagnostics is a biotechnology company based in Burlington, ON, Canada, and Tulsa, OK, USA. Our focus is on providing and developing nanotechnology derived products and services for the International Life Science and Material Science markets and has been doing so for 15+ years.

In addition to our two locations, Cytodiagnostics also has an associate company, CytoGroup, which specializes in lateral flow and vertical flow assays, and ELISA. Both products and development services are available.

Our Life Science portfolio contains products and services specifically tailored for both in vitro and in vivo research and assay development and include product lines such as:
Noble Metal Nanoparticles, Fluorescent Nanoparticles, Magnetic Nanoparticles, Assays, Custom Products and Research.


Products

Services

Nanoparticle Products

Nanoparticles have many applications in biology and medicine due to their unique optical and physical properties. These versatile reagents can be used for biosensor development, as cellular probes, as drug delivery vehicles, or as optical contrast agents among others.

Cytodiagnostics spherical and non-spherical nanoparticle products are made with proprietary protocols resulting in particles with uniform shapes and narrow size distributions.

By precisely engineering the nanoparticle surface, we also offer secondary antibody conjugates and particles with functional groups such as NHS, biotin, carboxyl, amine and methyl allowing them to be directly used in many applications.

Browse our available nanoparticle product categories below.

Nanoparticle Purification and Sample Preparation

Magnetic Nanoparticles

Lateral Flow Assay Products

The lateral flow immunoassay test, also known as immunochromatography assay, or strip test is an extremely versatile and fast method for visual detection of antigen in a sample. Lateral flow immunoassays are essentially immunoassays adapted to operate along a single axis to suit the test strip format but can also be operated in a vertical flow format.

The probes used in lateral flow and other similar assays such as vertical flow are commonly based on gold nanoparticle antibody conjugates. Due to the optical properties of noble metal nanoparticles such as gold, detection with the naked eye can be achieved with excellent sensitivity. The assay can also be adapted to run both in non-competitive and competitive mode.

For more information on rapid flow tests please read our vertical flow immunoassay or lateral flow immunoassays technical note or browse our products below.

Browse Available Product Categories Below

Assay Kits

ELISA Kits & Reagents

Immunoblotting Products

Antibody Detection Dipsticks and ELISA Kits

Vertical Flow Products

Services

Cytodiagnostics offers flexible solutions for development and manufacturing of lateral flow assays based on our noble-metal nanoparticles (standard gold nanoparticles and our unique gold nanourchins).

By leveraging years of experience in nanoparticle manufacturing and assay development we can reduce the time from project inception to a finished product. Whether it is a smaller project or full-scale assay development project with the goal of launching a commercial product, Cytodiagnostics is here to help.

Common service and project types:

  • Antibody conjugation and screening
  • New assay development
  • Transfer and optimization of existing proof-of-concept assay into a flow based rapid test setting.
  • Deposition of your gold conjugate on conjugate pads

If you are interested in our custom assay development services please contact us and we would be delighted to discuss your project.

Our ELISA Research and Development team has over 20 years of experience with immunoassay development, sample handling, data analysis, and proteomics. We employ high-quality validation and testing procedures to ensure all custom assays we develop perform according to desired specifications.

Is a suitable or affordable assay not commercially available for your target of interest?

Read below for more information on Cytodiagnostics ELISA development services or contact us today to discuss your project.

Custom Immunoassay Development and Services We Offer

  • Development of a unique ELISA specific to your target, with defined project milestones and progress reports to suit your needs.
  • QC testing
  • Bulk manufacturing

Required materials and reagents to begin assay development:

  1. Analyte
  2. Antibodies
  3. Analyte negative samples
  4. Analyte positive samples

Typical ELISA Development Project Phases

Step 1: Antibody Pair Identification and Assay Titration

  1. Selection of compatible capture and detection antibody pairs.
  2. Determination of an optimal capture and detection antibody conjugate pair providing optimal signal-to-noise ratio: titration experiments.
  3. Development and optimization of ELISA plate coating procedures for optimal antigen capture: titration experiment.

Step 2: Assay Optimization

  1. Selection of proper sample diluent for signal generation and detection.
  2. Assessment and elimination of sample matrix effects, i.e., interference or non-specific binding.
  3. Construction of a standard curve that reflects the characteristics of the sample matrix: Parallelism.
  4. Development of an assay protocol that can detect the analyte of interest within the target sensitivity range.

Step 3: Assay Validation

  1. Determining sensitivity and precision of the assay.
  2. Determining performance of the assay in various sample matrices: Spike-in and recovery and Linearity of dilution.
  3. Optimizing assay components and incubation protocols to meet final detection requirements.

Step 4. Production of Finished Kits

  1. Quality control assessment of final components.
  2. Assembly, packaging, and delivery of ELISA kit.

If you are interested in learning more about the services we offer or want to discuss your custom ELISA development project please contact us.

Product Literature


Magnetic Nanoparticles

View All Products Superparamagnetic iron oxide (Fe3O4, magnetite) nanoparticles are one of the most widely studied nanomaterials today. They are Read More...

Immunoblotting Products

View All Products Gold Protein Detection Kit Catalog No.DescriptionSR-07-01Gold Protein Detection Kit for MembranesGO-MEM-200Gold Nanoparticle Conjugation Optimization Kit - Dot-Blot Read More...

Vertical Flow Products

View All Products Vertical Flow Products Cytodiagnostics rapid test development kits are ideal for customers who want to improve the Read More...

Antibody Detection Dipsticks and ELISA Kits

View All Products Antibody Detection, Characterization & Quantification When working with pure proteins solutions and more complex mixtures, it is Read More...

ELISAs

View All Products ELISA Kits & Reagents What is an ELISA (enzyme-linked immunosorbent assay)? An ELISA (enzyme-linked immunosorbent assay) is Read More...

Lateral Flow Assay Products

View All Products Lateral Flow Assay ProductsUniversal Lateral Flow Assay KitAntibody Detection Dipstick Assay KitsAntibody Detection, Characterization & QuantificationLateral Flow Read More...

Iron Oxide Nanoparticles

View All Products Superparamagnetic iron oxide (Fe3O4, magnetite) nanoparticles are one of the most widely studied nanomaterials today. They are Read More...

Quantum Dots

View All Products Cytodiagnostics Cadmium Selenide/Zinc Sulfide (CdSe/ZnS) core/shell quantum dots are functionalized with either a small alkyl ligand (organic-soluble) Read More...

Gold Nanoparticles

View All Products Spherical Gold NanoparticlesGold Nanoparticle Conjugates and Kits Gold nanoparticles have many applications in biology and medicine due Read More...

Silver Nanoparticles

View All Products Spherical Silver NanoparticlesSilver Nanoparticle Conjugates and Kits Cytodiagnostics spherical silver nanoparticles are available with core sizes of Read More...

Gold NanoUrchins

View All Products Gold NanoUrchinsGold NanoUrchin Conjugates and Kits Cytodiagnostics Gold NanoUrchins have unique optical properties compared to spherical gold Read More...

Gold Nanorods

View All Products In addition to our functionalized spherical gold nanoparticles Cytodiagnostics is proud to offer functionalized gold nanorods. These Read More...

Technical Notes

Contact UsView All Products Browse our Available Tech Notes Below Our technical literature is made available in an effort to Read More...

Frequently Asked Questions

Contact UsView All Products Below are answers to the most frequently asked questions about our products and services. If you Read More...

First for Antibodies

Stratech Scientific have been successful in a recent antibodies tender from the Southern Universities Purchasing Consortium. In the award criterion Read More…

Gold Nanoparticle Handling and Storage

Storage Store product away from direct sunlight at 4-25°C. Lower temperature prolongs the shelf life of the product. Do NOT Read More…

Silver Nanoparticle Handling and Storage

  Storage Store silver nanoparticles at 2-8°C and protected from light. Do NOT freeze. If frozen, the silver nanoparticles will Read More…

Conjugation of Proteins to Zepto Ultra Express Fluorescent Microspheres

Zepto™ Ultra Express Microspheres are metal-coated fluorescent polystyrene beads designed for fast surface functionalization with molecules containing primary amines, tiol Read More…

Covalent Conjugation of Proteins to Carboxylated Gold Nanoparticles

Background Gold nanoparticle conjugates have been widely used in biological research and biosensing applications. For example, gold nanoparticles can function Read More…

Conjugation of Proteins to Silver Nanoparticles

Background Silver nanoparticle conjugates are useful for a wide range of biological applications including bio-sensing and bio-imaging. Stable silver nanoparticle Read More…