Products

Everest Biotech’s goat polyclonal antibodies are released either as Everest Elite Grade or Aspiring Grade products, depending on their performance in testing and the state of knowledge about the target protein. Everest Biotech offers high quality alternatives to the Santa Cruz polyclonal product range. Everest Biotech’s commitment to quality and continuous improvement has been recognised with ISO 9001:2015 accreditation (Certificate number CA14829, Quality policy ).

Elite Grade Antibodies

• Peptide ELISA data showing they recognise the immunizing peptide.
• Western blot data showing correct size band on physiologically relevant tissue or cell line.
• IHC/IF data and specific publications where available.
• Also available in bulk for assay developers.

 Aspiring Grade Antibodies

• Brought quickly to the market for targets that have few or no commercial antibodies available and for which there is little or no expression data in the literature.
• Supported by peptide ELISA data.
• Low background but wrong size band or no signal in the range of common cell lines and tissues available for testing.
• Sold at a significantly reduced price but we are not certain that they will recognize the native protein in any application.

Immunising Peptides

• Peptides used in production of antibodies available for blocking experiments.
• Sold as 100µg of Lyophilised peptide.

Secondary antibodies

First identified in Humans in the 1960’s, coronaviruses belong to the family Coronaviridae; which is one of three RNA virus families within the order Nidovirales. The family is comprised of two subfamilies, Coronavirinae and Torovirinae; with members of Coronavirinae being divided into the four genera of alpha, beta, gamma and delta. Both coronaviruses and toroviruses are positive-stranded, enveloped RNA viruses, and were previously associated with mild respiratory symptoms. However, since 2002 the severe acute respiratory syndrome coronavirus (SARS-CoV) and the Middle East respiratory syndrome coronavirus (MERS-CoV) have both been shown to cause severe respiratory diseases. The most recent coronavirus strain identified SARS-coronavirus 2 (SARS-CoV-2), has resulted in the current global pandemic coronavirus disease 2019 (COVID-19).
A beta coronavirus, SARS-CoV-2 shares approximately 80% of its RNA sequence with SARS-CoV. It has four major structural proteins: the nucleocapsid protein (N), membrane protein (M), spike protein (S) and envelope protein (E). The spike protein (S) consists of two subunits, S1 and S2. The S1 subunit contains a receptor binding domain, which attaches to host cellular membrane receptors, such as Angiotensin converting enzyme 2 (ACE2). The S2 subunit allows for membrane fusion. In order to enter the host cell, the S protein requires priming by host proteases, such as TMPRSS2. The spike glycoproteins also give the virus its distinctive crown-like appearance and name. The nucleocapsid (N) protein is expressed mainly in the cytoplasm, and it’s binding to the CoV genome, allows it to be packaged into the ribonucleoprotein complex (capsid). The membrane (M) protein defines the shape of the viral envelope. Finally, the envelope (E) protein is integrated into the virion envelope.

Everest Biotech has been manufacturing anti-peptide and antigen affinity purified goat polyclonal antibodies since 2000

Do you wish immunofluorescence of suspension cells was as easy as with adherent cells? Now it is! Shi-fix™ (manufactured by Shikhar Biotech) cover-slips allow suspension cells to be layered on them or directly grown as a monolayer. Easy, hassle-free protocol and no spin columns needed for fixing suspension cells to slides. Just add your cells to the coverslips, wait for 30 mins, wash unbound cells with PBS and proceed to immunostaining (or continue the culture to obtain desired cell densities).

These cover-slips can also be used for staining suspension cells for microscopy, or for fixing suspension cells for confocal microscopy. What’s more, you can also stimulate the cells while they are attached on the cover-slip, should you need to do so. You can work on non-adherent cells as if they were adherent!

Are you planning to develop an assay and require >1mg antibody?  Everest Biotech have stock of over 5000 affinity purified antibodies available in bulk:

HIGH SPECIFICITY AND AFFINITY

All antibodies have been antigen affinity purified thus selecting for high affnity.

GOAT RAISED ANTIBODIES

Large batches to evade batch-to-batch variations.

DEFINED EPITOPE

Generated  and affinity purified using 10-15aa peptides, sequence always on the datasheet.

TEST SAMPLES AVAILABLE

From reserved batches.

CHOICE OF FORMATS

Including BSA free, Azide free and conjugations.

IMMUNIZING PEPTIDES

Available on request.