* SC = secretory component, ND = not done, HP6019-HP6030-HP6058 are exclusion antibodies because they bind to three of four human IgG subclasses.

** Most popular clones for their respective specificities.

For the Human Immunoglobulins

*The human IgG subclass enzyme immunoassays may be developed with a biotinylated poly-monoclonal anti-human IgG reagent, anti-kappa/lambda light chain reagent or a polyclonal mouse anti-human IgG reagent.

Capture antibodies are to be used at 10 micrograms per ml (1:200 dilution) in PBS-no protein for coating and detection antibodies are to be used at 1 microgram per ml (1:1000 dilution) in PBS-1% bovine serum albumin.

Horseradish Peroxidase Labeled Monoclonal Antibodies

Monoclonal antibodies specific for human IgG PAN (IgG1/2/3/4), IgA PAN (IgA1/2) and human IgM have been directly conjugated with horseradish peroxidase (HRP) using the Nakane/Kawaoi procedure. These antibodies are supplied at 1 mg/ml in 0.5 ml aliquots and should be diluted from 1:2000 to 1:8000 for use in immunoassays. These antibodies are being used in an internationally documented protocol for the measurement of human IgG, IgA and IgM antibodies specific for pneumococcal polysaccharides.

Selected References Related to or Using HRL Monoclonal Antibodies

1. Jefferis R., CB Reimer, F Skvaril et al. Evaluation of monoclonal antibodies having specificity for human IgG subclasses. Results of an IUIS/WHO collaborative study. Immunol Lett 10:223, 1985.
2. Reimer CB et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma 3:236, 1984.
3. Phillips DJ, TW Wells, CB Reimer. Estimation of association constants of monoclonal antibodies to human IgG epitopes using fluorescent sequential saturation assays. Immunol Lett 17:159, 1987.
4. Hamilton RG. Human IgG subclass measurements in the clinical laboratory. Clin Chem 33:1070-5, 1987.
5. Hamilton RG. Human IgG Subclasses. Monograph, Calbiochem Corporation, pp 1-76, 1987.
6. Hamilton RG, RW Wilson, T Spillman, M Roebber. Monoclonal antibody-based immunoenzymetric assays for quantification of human IgG and its four subclasses. J Immunoassay 9:275-96, 1988.
7. Hamilton RG, M Roebber, CB Reimer, SL Rodkey. Isoelectric focusing patterns of mouse monoclonal antibodies to the four human IgG subclasses. Electrophoresis 9:127-34, 1987.
8. Hamilton RG, M Roebber, CB Reimer, SL Rodkey. Quality control of murine monoclonal antibodies using isoelectric focusing immunoblot analysis. Hybridoma 6:204-217, 1987.
9. Reimer CB. Five hydridomas secreting monoclonal antibodies against human IgE. Monoclonal Antibody News 4:2, 1986.
10. Jones CC, RG Hamilton, RE Jordon. Subclass distribution of human IgG autoantibodies in pemphigus. J Clin Immunol 8:43-9, 1988.
11. Fasullo FJ Jr, HA Fritsche Jr, F Liu, RG Hamilton. IgG heavy chain subclass typing of myeloma paraprotein by isoelectric focusing (IEF) and immunoblot analysis. Clin Chem 35:364-8, 1989.
12. Hamilton RG. Inter-laboratory quality control program for IgG subclass protein immunoassays in North America. In. Protides of the Biological Fluids, Pergamon Press, 1989.
13. Reimer CB, DJ Phillips, CH Aloisio, CM Black, TW Wells. Specificity and association constants of 33 monoclonal antibodies to human IgA epitopes. Immunol Lett 21:209, 1989.
14. Hamilton RG. Engineered human antibodies as immunologic quality control reagents. Ann Biol Clin 48:473-7, 1990.
15. Hamilton RG. Production and epitope location of monoclonal antibodies to the human IgG subclasses. In: Molecular Aspects of Immunoglobulin Subclasses. ed. F Shakib, Pergamon Press, Oxford, pp 79-91 (Chapter 5), 1990.
16. Hamilton RG. Application of engineered chimeric antibodies to the calibration of human antibody standards. Ann Biol Clin. 49:242-248, 1991.
17. Herrman DJ, RG Hamilton, T Barington CE Frash, G Arakere, O Makela, LA Mitchell, J Nael, GT Rijkers, B Zegers, B Danve, JI Ward, CS Brown. Quantitation of human IgG subclass antibodies to Haemophilus influenzae Type b capsular polysaccharide: Results of an international collaborative study using enzyme immunoassay methodology. J Imunol Meth 148:101-14, 1992.
18. Lichtenstein LM, A Kagey-Sobotka, JM White, RG Hamilton. Anti-human IgG causes basophil histamine release by acting on IgG-IgE complexes bound to IgE receptors. J Immunol 148:3929-36, 1992.
19. Wilson ME, RG Hamilton. IgG subclass response of localized juvenile periodontitis subjects to Actinobacillus actinomycetemcomitans Y4 lipopolysaccharide. Infection Immunity 60:1806-12, 1992.
20. Hamilton RG, SM Morrison. Epitope mapping of human IgG specific murine monoclonal antibodies with domain-switched, deleted and point-mutated chimeric antibodies. J Immunol Meth 158:107-22, 1993.
21. Batard T, B Basuyaux, P Lambin, C Bremard-Oury, RG Hamilton, B David, G Peltre. Isotypic analysis of grass pollen-specific immunoglobulins in human plasma. I. Specialization of certain classes and subclasses in the immune response. Intern Arch Allergy Immunol 10:68-73, 1993.
22. Kuppers RC, IM Outschoorn, RG Hamilton, CL Burek, NR Rose. Quantitative measurement of human thyroglobulin-specific antibodies by use of a sensitive enzyme-linked immunoassay. Clin Immunol Immunopath 67:1-10, 1993.
23. Peebles S, M Liu, LM Lichtenstein, RG Hamilton. IgA, IgG and IgM quantification in bronchoalveolar lavage fluids from allergic rhinitics, allergic asthmatics and normal subjects by monoclonal antibody-based immunoenzymetric assays. J. Immunol Meth. 179:77-86, 1995.
24. Wilson ME, RG Hamilton. IgG2 antibodies promote neutrophil killing of Actinobacillus actinomycetemcomitans. Infection Immunity 63:1070-1075, 1995.
25. Kleine-Tebbe J, RG Hamilton, M. Roebber, LM Lichtenstein, SM MacDonald. Purification of immunoglobulin E (IgE) antibodies from sera of high IgE titers. J. Immunol Meth. 179:153-164, 1995.
26. Wilson ME, RG Hamilton. IgG subclass response on juvenile periodontitis subjects to principal outer membrane proteins of Actinobacillus actinomycetemcomitans. Infection Immunity 63:1062-1069, 1995.
27. Madore DV, P Anderson, BD Baxter, GM Carlone, KM Edwards, RG Hamilton. Inter-laboratory study evaluating quantitation of antibodies to Haemophilus influenzae type b polysaccharide by ELISA. Clinical and Diagnostic Laboratory Immunology 3:84-88, 1996.
28. Mestecky J. RG. Hamilton, CGM Magnusson, R Jefferis, JP Vaerman, M Goodall, GG de Lange, I Moro, P Aucouturier, J Radl, C Cambiaso, C Silvain, JL Preud’homme, K Kusama, GM Carlone, J Biewenga, K Kobayashi, CB Reimer. Evaluation of monoclonal antibodies with specificity for human IgA, IgA subclasses and allotypes and secretory component. Results of an IUIS/WHO collaborative study. J. Immunol. Meth. 193(2):103-148, 1996.
29. Hamilton RG. N.F. Adkinson Jr. Immunological tests for the diagnosis and management of human allergic disease: Total and allergen-specific IgE and allergen specific IgG. In: Manual of Clinical Laboratory Immunology. ed. HR Rose, EC de Macario, JL Fahey, H Friedman, GM Penn. American Society for Microbiology, Washington D.C., Chapter 102, 1997.
30. Peebles RS, MC Liu, NF Adkinson Jr., LM Lichtenstein, RG Hamilton. Ragweed specific antibodies in bronchoalveolar lavage fluids and serum before and after segmental lung challenge: IgE and IgA associated with eosinophil degranulation. J Allergy Clin Immunol 101:265-73, 1998.
31. King CJ, W Borkowsky, N Mahidhara, ED Shapiro, RM Rutstein, NQ Tan, JJ Farley, WM Dankner, S Nachman, E Simoes, PM Flynn, J Clemens, A Kimura, RG Hamilton. Group specific antibody levels surrounding invasive pneumococcal illness in children infected with human immunodeficiency virus. J. Infect. Dis. 181-1817-21, 2000.
32. Peebles RS, RG Hamilton, LM Lichtenstein, M Schlosberg, MC Liu, D Proud, A Togias. Antigen specific IgE and IgA antibodies in bronchoalveolar lavage fluid are associated with stronger antigen-induced late phase reactions. Clin. Exp. Allergy 31:239-48,2001.