NZYtech’s Custom Gene Synthesis service provides in vitro chemical synthesis, cloning and 100% sequence verification of any desired DNA genetic sequence.

NZYtech’s Custom Gene Synthesis service provides in vitro chemical synthesis, cloning and 100% sequence verification of any desired DNA genetic sequence. NZYtech’s R&D department improved standard molecular biology processes and developed innovative bio-informatics tools for gene design and codon optimization. Our proprietary gene optimization algorithm – ATGenium – comprehensively optimizes critical factors in transcription, translation and co-translational protein folding to deliver highest levels of expression in any host system.
Synthetic genes can be cloned into our standard cloning vectors (pNZY30), into pHTP Escherichia coli expression vectors or into a selection of other bacterial, mammalian and viral expression vectors. Check the complete list of vectors below.

Features:

– Free gene design & codon optimization
– Flexible gene design process: addition of tags, restriction sites or other elements
– 100% sequence accuracy guaranteed
– Cloning vector (pNZY30) with Ampicillin or Kanamyciin resistance
– Possibility of cloning your synthetic gene directly into a selection of bacterial, mammalian and viral expression vectors, namely into pHTP E. coli expression vectors (for an additional price).
– Complete confidentiality guaranteed

Delivery Specifications:
– 40-4000 ng of purified plasmid
– Gene Synthesis Report

Codon optimization of your genes

Proteins play a variety of critical roles in many biological processes. They are valuable therapeutic compounds, research reagents and industrial catalysts. NZYtech’s proprietary codon optimization software – ATGenium – can alter both naturally occurring and recombinant gene sequences to achieve the highest possible levels of protein expression in any given expression system. This will save you time and money, while getting you quickly to meaningful results. ATGenium takes into consideration a variety of critical factors involved in different stages of protein expression, such as codon adaptability, mRNA structure, and various cis-elements in transcription and translation. Optimizing your gene sequence will have significant advantages in a variety of applications such as recombinant protein expression, DNA vaccine design and protein engineering.

Optimizing heterologous protein production in Escherichia coli with ATGenium:
Superior levels of recombinant proteins expressed in Escherichia coli strains can be achieved through codon optimization using the NZYtech’s ATGenium algorithm.

A. Levels of protein expression of three different eukaryotic proteins in a prokaryotic system (Escherichia coli). The genes encoding these proteins were optimized by three algorithms for codons optimization, by two different competitors (light blue) and using the ATGenium algorithm developed by NZYtech (dark blue).

B. High-throughput recombinant protein expression of CAZYmes (Carbohydrate Active eNZymes). Genes encoding these proteins were optimized using the ATGenium algorithm, cloned into pHTP1 expression vector and overexpressed incoli BL21 (DE3) strain. Recombinant proteins were purified through IMAC and separated by SDS-PAGE. High protein expression yields were obtained when genes were optimized using the ATGenium algorithm

NZYtech offers a fast and cost-effective Gene Fragments service providing double-stranded DNA sequences between 300-1800 bp in length. Gene Fragments constitute a simple option to PCR-based procedures and can be further used in every downstream cloning methods

Gene Fragments sequences can be optimized using NZYtech’s proprietary gene optimization algorithm – ATGenium – which comprehensively optimizes critical factors in transcription, translation and co-translational protein folding to deliver highest levels of expression in any host system.

Features:

– Free gene design & codon optimization
– Flexible gene design process: addition of tags, restriction sites or other elements
– Sequences length between 300 bp and 1800 bp
– Gene fragments size verification by electrophoresis
– Non-complex sequences only
– Considerable saving of time and money
– Complete confidentiality guaranteed

Delivery Specifications:

– 100-1000 ng dry dsDNA fragment
– ≤10 gene fragments delivered in tube; >10 genes delivered in plate
– Gene Fragments Report

Codon optimization of your genes

Proteins play a variety of critical roles in many biological processes. They are valuable therapeutic compounds, research reagents and industrial catalysts. NZYtech’s proprietary codon optimization software – ATGenium – can alter both naturally occurring and recombinant gene sequences to achieve the highest possible levels of protein expression in any given expression system. This will save you time and money, while getting you quickly to meaningful results. ATGenium takes into consideration a variety of critical factors involved in different stages of protein expression, such as codon adaptability, mRNA structure, and various cis-elements in transcription and translation. Optimizing your gene sequence will have significant advantages in a variety of applications such as recombinant protein expression, DNA vaccine design and protein engineering.

Optimizing heterologous protein production in Escherichia coli with ATGenium:
Superior levels of recombinant proteins expressed in Escherichia coli strains can be achieved through codon optimization using the NZYtech’s ATGenium algorithm.

A. Levels of protein expression of three different eukaryotic proteins in a prokaryotic system (Escherichia coli). The genes encoding these proteins were optimized by three algorithms for codons optimization, by two different competitors (light blue) and using the ATGenium algorithm developed by NZYtech (dark blue).

B. High-throughput recombinant protein expression of CAZYmes (Carbohydrate Active eNZymes). Genes encoding these proteins were optimized using the ATGenium algorithm, cloned into pHTP1 expression vector and overexpressed incoli BL21 (DE3) strain. Recombinant proteins were purified through IMAC and separated by SDS-PAGE. High protein expression yields were obtained when genes were optimized using the ATGenium algorithm

For orders above 48 synthetic genes, NZYtech’s R&D team developed a High-throughput (HTP) Gene Synthesis platform to efficiently generate hundreds to thousands of synthetic genes

NZYtech’s R&D team developed a High-throughput (HTP) Gene Synthesis platform to efficiently generate hundreds to thousands of synthetic genes. With that in mind, we have improved standard molecular biology processes and developed innovative bioinformatics tools for gene design and codon optimization. HTP Gene Synthesis will allow you to tackle difficult research projects such as those involving determining structure-function relationships in different proteins, creating novel antibody libraries, screening gene variant libraries or optimizing protein function and expression. Our codon optimization technology – ATGenium – will allow obtaining the highest possible levels of gene expression in different expressions hosts while retaining high levels of protein solubility.
Synthetic genes can be cloned into our standard cloning vectors (pNZY30), into NZYtech’s pHTP Escherichia coli expression vectors or into a selection of other bacterial, mammalian and viral expression vectors. Check the complete list of vectors below.

Features:

– Free gene design & codon optimization
– Flexible gene design process: addition of tags, restriction sites or other elements
– 100% sequence accuracy guaranteed
– Cloning vector (pNZY30) with Ampicillin or Kanamyicin resistance
– Possibility of cloning your synthetic gene directly into a selection of bacterial, mammalian and viral expression vectors, namely into NZYtech’s pHTP E. coli expression vectors (for an additional price)
– Complete confidentiality guaranteed

Delivery Specifications:

– 100-1000 ng dry dsDNA fragment
– ≤10 gene fragments delivered in tube; >10 genes delivered in plate
– Gene Fragments Report

Codon optimization of your genes

Proteins play a variety of critical roles in many biological processes. They are valuable therapeutic compounds, research reagents and industrial catalysts. NZYtech’s proprietary codon optimization software – ATGenium – can alter both naturally occurring and recombinant gene sequences to achieve the highest possible levels of protein expression in any given expression system. This will save you time and money, while getting you quickly to meaningful results. ATGenium takes into consideration a variety of critical factors involved in different stages of protein expression, such as codon adaptability, mRNA structure, and various cis-elements in transcription and translation. Optimizing your gene sequence will have significant advantages in a variety of applications such as recombinant protein expression, DNA vaccine design and protein engineering.

Optimizing heterologous protein production in Escherichia coli with ATGenium:
Superior levels of recombinant proteins expressed in Escherichia coli strains can be achieved through codon optimization using the NZYtech’s ATGenium algorithm.

A. Levels of protein expression of three different eukaryotic proteins in a prokaryotic system (Escherichia coli). The genes encoding these proteins were optimized by three algorithms for codons optimization, by two different competitors (light blue) and using the ATGenium algorithm developed by NZYtech (dark blue).

B. High-throughput recombinant protein expression of CAZYmes (Carbohydrate Active eNZymes). Genes encoding these proteins were optimized using the ATGenium algorithm, cloned into pHTP1 expression vector and overexpressed incoli BL21 (DE3) strain. Recombinant proteins were purified through IMAC and separated by SDS-PAGE. High protein expression yields were obtained when genes were optimized using the ATGenium algorithm

For orders above 48 synthetic genes, NZYtech’s R&D team developed a High-throughput (HTP) Gene Synthesis platform to efficiently generate hundreds to thousands of synthetic genes

NZYtech’s R&D team developed a High-throughput (HTP) Gene Fragments platform to efficiently generate hundreds to thousands of synthetic genes. With that in mind, we have improved standard molecular biology processes and developed innovative bioinformatics tools for gene design and codon optimization. HTP Gene Fragments will allow you to tackle difficult research projects such as those involving determining structure-function relationships in different proteins, creating novel antibody libraries, screening gene variant libraries or optimizing protein function and expression. Our codon optimization technology – ATGenium – will allow obtaining the highest possible levels of gene expression in different expressions hosts while retaining high levels of protein solubility.

Features:

– Free gene design & codon optimization
– Flexible gene design process: addition of tags, restriction sites or other elements
– Sequences length between 300 bp and 1800 bp
– Gene fragments size verification by electrophoresis
– Non-complex sequences only
– Considerable saving of time and money
– Complete confidentiality guaranteed

Delivery Specifications:

– 100-1000 ng dry dsDNA fragment (in plate)
– Gene Fragments Report

Codon optimization of your genes

Proteins play a variety of critical roles in many biological processes. They are valuable therapeutic compounds, research reagents and industrial catalysts. NZYtech’s proprietary codon optimization software – ATGenium – can alter both naturally occurring and recombinant gene sequences to achieve the highest possible levels of protein expression in any given expression system. This will save you time and money, while getting you quickly to meaningful results. ATGenium takes into consideration a variety of critical factors involved in different stages of protein expression, such as codon adaptability, mRNA structure, and various cis-elements in transcription and translation. Optimizing your gene sequence will have significant advantages in a variety of applications such as recombinant protein expression, DNA vaccine design and protein engineering.

Optimizing heterologous protein production in Escherichia coli with ATGenium:
Superior levels of recombinant proteins expressed in Escherichia coli strains can be achieved through codon optimization using the NZYtech’s ATGenium algorithm.

A. Levels of protein expression of three different eukaryotic proteins in a prokaryotic system (Escherichia coli). The genes encoding these proteins were optimized by three algorithms for codons optimization, by two different competitors (light blue) and using the ATGenium algorithm developed by NZYtech (dark blue).

B. High-throughput recombinant protein expression of CAZYmes (Carbohydrate Active eNZymes). Genes encoding these proteins were optimized using the ATGenium algorithm, cloned into pHTP1 expression vector and overexpressed incoli BL21 (DE3) strain. Recombinant proteins were purified through IMAC and separated by SDS-PAGE. High protein expression yields were obtained when genes were optimized using the ATGenium algorithm

NZYtech offers a site-directed mutagenesis service which includes the introduction of single or multiple mutations – substitutions, insertions or deletions – into existing plasmid DNA sequences.

NZYtech offers a site-directed mutagenesis service which includes the introduction of single or multiple mutations – substitutions, insertions or deletions – into existing plasmid DNA sequences. The strategy usually involves an oligonucleotide-directed mutagenic approach. All generated mutant clones are screened by Sanger sequencing to ensure that the desired modifications have been made.

Features:

– Substitution, deletion or insertion within a known plasmid DNA sequence
– Use of high-fidelity enzymes to minimize PCR errors
– 100% accuracy of the mutated region
– Short turnaround time
– Complete confidentiality guaranteed

Delivery Specifications:

– 40-4000 μg purified mutant plasmid
– Site-directed mutagenesis report containing QC certificate

NZYtech peptides may be provided crude, desalted, 75%, 85%, 95% or 98% pure. NZYtech peptides are competitively priced. Customers retain all rights to the sequence data and related intellectual property.

NZYtech peptides may be provided crude, desalted, >75%, >85%, 95% or >98% pure. NZYtech peptides are competitively priced, starting at 3.82€/amino acid. Customers retain all rights to the sequence data and related intellectual property.

Features:

– Several purity levels available: crude, desalted, >75%, >85%, 95% or >98%
– Several modifications available: phosphorylation, methylation, acetylation, amidation, fluorescence/dye labeling

Delivery Specifications:

– Lyophilized peptide of the required sequence, purity, and quantity
– QC certificate