SBI is a small biotech company that’s delivered innovative products and services for molecular and cell biology research since January 2004, when they sold their first lentivectors and pPACK lentivirus packaging systems.

They focus on being the first company to bring the most recent methodologies to market—they were the first company to deliver exosome research reagents and CRISPR/Cas9 vectors—so researchers could quickly take advantage of the latest, most powerful approaches.

Dedicated to quality, many of their products and most of their service offerings are completed in their state-of-the-art facility in Palo Alto CA, enabling them to maintain control over manufacturing conditions and quality, ensure their service projects remain confidential and deliver frequent and timely service project status updates.

Their experienced staff includes a high proportion of PhD-level scientists who interact directly with customers to provide product and services support.


Product Categories

Supporting all aspects of your exosome research, SBI’s products enable quantitative, high-throughput, and high quality studies and deliver unparalleled capabilities

Get everything you need for studying and using exosomes, from isolation and characterization, to cargo profiling and engineering, and more. Whatever methods you choose—whether you use ExoQuick or density gradient ultracentrifugation, want qPCR-based exosomal miRNA profiling or RNA-Seq methods—we’ve got the high-quality exosome research products to support your science and advance your work.

Pairing flexibility and choice with experienced scientific support, our wide range of CRISPR/Cas9 products put the power of genome engineering into your hands

Ready to use CRISPR/Cas9? Then SBI’s ready for you. With a comprehensive selection of CRISPR/Cas9 products for genome engineering—including Cas9 and gRNA delivery systems in a flexible range of formats, homologous recombination donor vectors, and AAVS1 Safe Harbor targeting—we can bring your genome engineering projects to life. Even better, our experienced technical support team is available to answer your questions on vector design, experimental setup, and more—just use the Contact page to start the conversation.

Widely regarded in the industry for delivering high-quality, high titer lentivirus preparations, SBI’s Lentivirus Production Products power cutting edge research across a range of applications

Whatever type of cell you’re trying to transduce, from 293T cells to the more difficult types like T cells and B cells, quality matters. Which is why SBI offers optimized virus packaging systems and reagents that can help you reliably obtain high titer preparations and efficient transfection and transduction.

Offering integrating and non-integrating vectors in viral and non-viral systems, SBI offers a wealth of options for gene delivery and expression

With a wealth of options for gene delivery and expression, SBI has the gene expression vectors to support a wide variety of projects. We offer viral and non-viral systems that can integrate into the genome or remain episomal as well as inducible systems for highly controlled expression. Not quite sure what you need? Contact one of our technical support scientists—use the form on the Contact page to get the conversation started.

Delivering unparalleled insight into the cell, SBI’s wide range of Imaging & Reporter Vectors provide ready-built, ready-to-use constructs for today’s busy labs

Gain clearer insight into the inner workings of the cell with one or more of SBI’s many Imaging & Reporter Vectors. Visualize subcellular compartments and track physiological processes such as differentiation, signal transduction, apoptosis, and more with our robust, reliable constructs.

From profiling to overexpression for biomarker discovery, basic and translational research, and more, SBI’s got the long and short of non-coding RNA covered

No matter the application, whether you’re checking individual miRNA or lncRNA expression levels or profiling a range of constructs, knocking-down or overexpressing miRNAs, SBI’s got your miRNA research and lncRNA research tools covered. Select from a wide-range of pre-made expression or knockdown constructs, or use one of our expertly curated profiling arrays.

For comprehensive, end-to-end exosome services that include expert technical and data interpretation support from a highly experienced team, you can rely on SBI

CRISPR/Cas9 Services
SBI’s CRISPR/Cas9 Services can cover all or just part of your entire genome engineering workflow

  • CRISPR/Cas9 gRNA and HR Donor Cloning
  • CRISPR/Cas9 Cell Line Engineering

Minicircle Services
Get sustained transgene expression free from foreign DNA

RNA Services
SBI’s services team is ready to support your RNA studies

  • mRNA Synthesis Services
  • miRNA & lncRNA Profiling Services
  • Custom miRNA Precursor and anti-miRNA (miRZip) Services

Syn2Clone Custom Constructs
Affordable and easy custom plasmid construction

Exosome Services
Comprehensive, end-to-end exosome services

  • Exo-NGS
  • Exosome Proteomics Services
  • Exosome Lipidomics & Metabolomics Services
  • NTA-based Exosome Characterization
  • Exosome Isolation & Manufacturing
  • Exosome Engineering

Lentivirus Packaging
Get high-titer, transduction-ready lentivirus in as little as ten days

Stable Cell Lines
Get custom reporter, overexpression, or other type of cell line fast and hassle-free

  • Custom Overexpression Stable Cell Lines
  • Custom Luciferase-labeled Stable Cell Lines
  • Reporter Cell Lines

For comprehensive, end-to-end exosome services that include expert technical and data interpretation support from a highly experienced team, you can rely on SBI

When you choose SBI’s Exosome Services, you get more than high-quality exosome production or simple data delivery, you get in-depth scientific and technical support on everything from project planning to data interpretation from scientists with years of exosome experience. Whatever your exosome question, whatever your project—from exosome characterization to engineering—you can rely on the services team at SBI to not only deliver your data but to deepen your insights and advance your science.

If you don’t see the exosome service you need below, don’t hesitate to contact us with your project needs—our scientific team is happy to develop and deliver custom exosome projects for biomarker studies and more.

Exo-NGS

Overview

Getting better insights from exosomal RNA biomarkers is as easy as S-B-I

Because we know exosomes and we know how to deliver on end-to-end service

For many researchers interested sequencing exosomal RNA—whether it’s for biomarker discovery and profiling, or addressing other research questions—it may seem like there are too many hurdles to overcome to get to the data: How much sample is required? What’s the best way to isolate exosomes? Does this data look right?

 

Fortunately, SBI is here to cut through your uncertainties by providing a customized service with full levels of support. Whether you’re an exosome expert or brand new to the field, SBI’s Exo-NGS (Exosomal RNA-Seq) services can accelerate your biomarker discovery and profiling.

Because we’ve been working with exosomes for years, we can reliably and reproducibly isolate exosomes from almost any biofluid—from plasma and tissue culture media to CSF, synovial fluid, and even mouse bronchial alveolar fluid (a challenging sample where exosomes are concerned!).

And more importantly for exosome RNA-Seq, we routinely generate high-quality exosomal RNA libraries from as little 1 ng of total RNA, which is unparalleled given the challenges of obtaining high-quality RNA from exosomes. SBI can overcome these experimental hurdles to deliver quality and meaningful NGS data from your exosome samples.

Lastly, but not least, looking beyond the data, we can provide what your average NGS company cannot: the expertise of working with exosomes and exosomal biomarker samples. We’ve run thousands of samples and know what to expect from exosome RNA-Seq studies. We don’t just deliver raw data, we provide analyzed files and typically spend time with you reviewing your results and providing expert insights into your data, a value-add that is rare in this industry. Still not sure? Take a look at the data below, and then contact us and we’ll be happy to answer any questions before, during, or after your Exo-NGS service.

  • More usable reads for uncovering novel biomarkers
  • Any type of exosomal RNA
  • Low sample requirements (as little as 1ng of total RNA)
  • Any biofluid (we handle exosome isolation)
  • Unparalleled end-to-end scientific support
  • Have your own exosomes? We can handle them!

 

How it works

Ordering Exo-NGS Services is easy

 

Step 1. Contact us for a quote.

Step 2. Send us your samples.

Step 3. Get your data files and a web-based presentation of the findings.

 

Supporting data

Good exosomal RNA-seq data starts with as little as 1 ng of total RNA

 

How do we get quality NGS data from such small amounts of RNA? It starts with the library prep. The standard methods for preparing RNA libraries often results in a large overlap between adaptor dimers and exosomal RNAs (Figure 1A). However, at SBI, we’ve modified the library prep to better separate the exosomal RNA from uninformative adaptor sequences (Figure 1B). The result is more usable sequencing reads from lower amounts of RNA as seen by the number (Figure 2) and percentage (Figure 3) of usable reads as well as the number (Figure 4) and percentages (Figure 5) of mapped reads. Fewer adaptors and more exosomal reads mean better insights from your data.

SBI’s exosomal RNA library preps provide better separation between exosomal RNA and the uninformative adaptor dimers

 

Figure 1. SBI’s exosomal RNA library preps provide better separation between exosomal RNA and the uninformative adaptor dimers.(A) Using standard library preparation methods, the adaptor dimer band overlaps with the exosomal RNA band, leading higher levels of contaminating adaptor sequence reads and fewer usable exosomal RNA reads. (B) SBI’s library preparation method leads to better separation between the adaptor sequences and the exosomal RNA, resulting in a higher percentage of usable exosomal RNA sequence reads.

 

 

SBI’s exosomal RNA library preps result in more total reads and more usable reads

 

Figure 2. SBI’s exosomal RNA library preps result in more total reads and more usable reads after quality assessment and control (QA/QC) filtering.

 

SBI’s exosomal RNA library preps result in a higher percentage of usable reads

 

Figure 3. SBI’s exosomal RNA library preps result in a higher percentage of usable reads.

 

SBI’s exosomal RNA library preps result in a higher number of mapped reads

 

Figure 4. SBI’s exosomal RNA library preps result in a higher number of mapped reads.

 

SBI’s exosomal RNA library preps result in a higher percentage of mapped reads

 

Figure 5. SBI’s exosomal RNA library preps result in a higher percentage of mapped reads.

Exosome Proteomics Services

Overview

Easily expand your biomarker studies to exosomal proteins

Because we know exosomes and we know how to deliver on end-to-end service

SBI’s Proteomics Services makes it easy to profile exosomal proteins, whether you’re hunting for biomarkers, seeking easy-to-access information on the physiology of the parent cell, or studying exosome biology. Our customized services provide comprehensive support that includes a pre-service consultation and everything from exosome isolation to data acquisition, analysis, and even interpretation. The result is high quality exosomal proteomics data regardless of whether you have a lot of experience working with exosomes or are new to the field.

 

Because we’ve been working with exosomes for years, we can reliably and reproducibly isolate exosomes from almost any biofluid—from plasma and tissue culture media to CSF, synovial fluid, and even mouse bronchial alveolar fluid (a challenging sample where exosomes are concerned!). And importantly for researchers working with limited sample volumes, our sample input requirements are very low (Table 1).

Table 1. Required sample volumes for SBI’s Proteomics Services

 

We can also work with already isolated exosomes, although we recommend using our proven sample preparation approaches which typically deliver cleaner peptide libraries with lower amounts of carryover protein, for more reliable exosome proteomics data.

Lastly, but not least, looking beyond the data, we can provide what your average proteomics service company cannot: the expertise of working with exosomes and exosomal biomarker samples. We’ve run thousands of samples and know what to expect from exosome proteomics studies. We don’t just deliver raw data, we provide analyzed files and typically spend time with you reviewing your results and providing expert insights into your data, a value-add that is rare in this industry. Still have questions? Ready to order? Contact us

Proteomics service highlights:

  • Proprietary sample cleanup procedure reduces non-specific proteins found in biofluids
  • Both EV total and surface protein profiling services available
  • Libraries prepared using standard trypsin cleavage for MASCOT compatibility
  • Data file compatibility with Scaffold viewer simplifies data mining and analysis
  • Fast turn-around times—approximately 4 weeks

Data deliverables:

 

How it works

Ordering Exosome Proteomics Services is easy

 

Step 1. Contact us for a quote.

Step 2. Send us your samples.

Step 3. Get your data files and a web-based presentation of the findings.

Exosome Lipidomics & Metabolomics Services

Overview

Take your biomarker studies to the next level with exosomal lipidomics/metabolomics

Because we know exosomes and we know how to deliver on end-to-end service

Whether you’re interested in circulating biomarker discovery, basic exosome research, or other exosome-related studies, SBI’s Exosome Lipidomics & Metabolomics Service helps you quickly and efficiently get the most information from your exosomes. Simply send us your sample or purified exosomes and we’ll send back a spreadsheet with putative identifications, mass/charge ratios, and differential analysis (if requested).

 

What can lipidomics of exosomes tell you?

Lipids are an important part of cellular physiology, and are increasingly being recognized for their importance in exosome biology as well. Exosomes were recently shown to have the highest lipid-to-protein ratio of all classes of extracellular vesicles1, with lipid content that both differs from the parent cell the vesicles are shed from2 and also changes as exosomes undergo a variety of physiological processes3. These unique lipid profiles can serve as novel circulating biomarkers, and recent evidence suggests that specific lipid species carried by exosomes can also modulate the function of recipient cells4.

With so much information revealed by lipid content, lipidomics studies of exosomes are a great way to identify lipid-based biomarkers and for understanding vesicle biogenesis and function5.

What can metabolomics of exosomes tell you?

In addition to lipids, exosomes carry a wide range of metabolites that can also be used for biomarker discovery. A recent study found distinctly different populations of metabolites in PANC1 cells treated with TGF-β, a known driver of cancer progression, versus a control group, suggesting that metabolite profiling can be used for differentiating cellular states6. Like exosomal lipids, exosomal metabolites can also take an active role in the target cell, and have been shown to reprogram metabolic machinery upon uptake by cancer cells, fueling growth7.

Lipidomics & Metabolomics service highlights:

  • Discover novel circulating biomarkers
  • Learn more about exosome biology
  • Send us your sample and receive data in 4–6 weeks

Data deliverables:

  • MS data will be delivered as Excel files with putative identification based on the m/z ratio of the analytes
  • Differential analysis of lipids and metabolites between treatment groups will be included in the Excel files

Exosomes affect metabolism in cancer cells

 

Figure 1. Exosomes affect metabolism in cancer cells. A recent study by Zhao, et al,7 demonstrated that exosomes from patient-derived cancer-associated fibroblasts (CAFs) can reprogram the cellular machinery in cancer cells. They used a metabolomics approach to demonstrate that exosomes shed from CAFs contain intact metabolites that fuel tumor growth. In addition, they showed that uptake of CAF exosomes inhibited mitochondrial oxidative phosphorylation, increasing glycolysis and glutamine-dependent reductive carboxylation.

 

References

  1. Osteikoetxea X, et al. Improved characterization of EV preparations based on protein to lipid ratio and lipid properties. PLoS One. 2015;10(3):e0121184. PMCID: PMC4370721.
  2. Llorente A, et al. Molecular lipidomics of exosomes released by PC-3 prostate cancer cells. Biochim Biophys Acta. 2013;1831(7):1302-9. PMID: 24046871.
  3. Carayon K, et al. Proteolipidic composition of exosomes changes during reticulocyte maturation. J Biol Chem. 2011;286(39):34426-39. PMCID: PMC3190795.
  4. Subra C, et al. Exosomes account for vesicle-mediated transcellular transport of activatable phospholipases and prostaglandins. J Lipid Res. 2010;51(8):2105-20. PMCID: PMC2903822.
  5. Subra C, et al. Exosome lipidomics unravels lipid sorting at the level of multivesicular bodies. Biochimie. 2007;89(2):205-12. PMID: 17157973.
  6. Altadill T, et al. Enabling Metabolomics Based Biomarker Discovery Studies Using Molecular Phenotyping of Exosome-Like Vesicles. PLoS One. 2016 Mar 14;11(3):e0151339. PMCID: PMC4790956.
  7. Zhao H, et al. Tumor microenvironment derived exosomes pleiotropically modulate cancer cell metabolism. Elife. 2016 Feb 27;5. pii: e10250. PMCID: PMC4841778.

 

How it works

Ordering Exosome Proteomics Services is easy

Step 1. Contact us for a quote.

Step 2. Send us your samples.

Step 3. Get your data files in 4–6 weeks.

Our standard service offers UPLC-FTMS analysis of very polar metabolites in the aqueous phase, with lipids and other metabolites in the organic phase. Non-polar analysis also available upon request.

Note that all IDs are based only on retention time, accurate mass measurement, and preliminary MS/MS data, and cannot be guaranteed. Not all peaks will be identified, and we recommend that any peaks of interest—either due to the identity of the compound reported and/or change in intensity between samples—MUST be verified by additional targeted analysis at additional cost.

Input sample requirements

 

 

Supporting data

See SBI’s Lipidomics & Metabolomics Service in action

 

Pathways represented by metabolites that are enriched in exosomes from an AML patient

 

Figure 2. Sample data—pathways represented by metabolites that are enriched in exosomes from an AML patient.from an AML patient. Exosomes were harvested from 300 μL of serum from both a healthy human subject and a subject with Acute Myeloid Leukemia (AML). Discovery-based lipidomic/metabolomics profiling was performed on the serum vesicles from the two subjects, and differentially expressed metabolites found.

NTA-based Exosome Characterization

 

Overview

Get the confidence to move forward with SBI’s NTA Services

Because we know exosomes and we know how to deliver on end-to-end service

 

 

 

 

 

When you use SBI’s NTA-based Exosome Characterization Services, you can get a clearer picture of the quality of your exosomes in as little as two weeks and without needing to invest in your own NTA instrument. Send us your biofluid or prepared exosomes, choose conventional or fluorescent nanoparticle tracking analysis (NTA), and we’ll send back a report with the particle analysis data—the mean and mode diameter size, particle concentration, and video of the collected data, all gathered in triplicate.

 

Why choose SBI for NTA characterization of exosome preps?

Because we’ve been working with exosomes for years, and can reliably and reproducibly isolate exosomes from almost any biofluid—from plasma and tissue culture media to CSF, synovial fluid, and even mouse bronchial alveolar fluid (a challenging sample where exosomes are concerned!).

And because SBI is the only company that can provide fluorescent NTA using our proprietary ExoGlow™-NTA reagents (learn more about conventional and fluorescent NTA below, in the How It Works section).

Nanoparticle Tracking Analysis-based Exosome Characterization Services highlights:

  • Understand the quality of your exosome preps
  • Leverage SBI’s long-term experience working with exosomes
  • Send us your biofluid or exosome prep and receive data in about 2 weeks

 

How it works

Ordering NTA-based Exosome Characterization Services is easy

 

Step 1. Contact us for a quote.

Step 2. Send us your biofluid or exosome prep.

Step 3. Get your data files in approximately 2 weeks.

What’s NTA?

SBI uses NTA measurements that rely on light scattering to extract particle size and the number of particles in a sample (Figure 1A). Briefly, a laser is directed at the sample and the particles in the sample scatter the light, which is imaged by a camera attached to a microscope and operating at 30 frames per second. The NTA software collects data on multiple particles simultaneously, and calculates the hydrodynamic diameter of each particle using the Stokes-Einstein equation.

How conventional and fluorescent NTA work

 

Figure 1. The ExoGlow-NTA dye only binds to membranes of intact EVs. Unlike conventional NTA (A), which collects data on all particles in a solution based on light scattering, the fluorescence mode of the NTA instrument selectively detects only the fluorescently-labeled particles—in this case, the ExoGlow-NTA-labeled EVs (B)—and only the data from fluorescently-labeled particles is reported.

 

What is fluorescent NTA and how is it different from conventional NTA?

Making a great exosome research tool even better, SBI has developed ExoGlow-NTA, a proprietary dye for fluorescent NTA* that enables data collection for only the extracellular vesicles present in a heterogenous sample. The result is more accurate EV/exosomal NanoSight data that ignores protein aggregates, membrane fractions, and other background particles to provide EV-specific particle size and concentration.

The ExoGlow-NTA reagent works by binding specifically and efficiently to the membranes of intact EVs, including exosomes. During data collection for fluorescent NTA, only the light scattering from fluorescently-labeled particles is obtained, resulting in EV-specific signal that ignores membrane fragments, protein aggregates, and other background particles since they do not bind the ExoGlow-NTA dye (Figure 1B). Thus, with the ExoGlow-NTA Kit, the data delivered by the NTA analysis more accurately reports on the EVs in your sample, rather than all of the particles in your sample.

 

Supporting data

Get Better NTA data on EVs using ExoGlow-NTA

 

SBI’s ExoGlow-NTA Fluorescent Labeling Kit delivers highly accurate EV quantitation in a quick and easy workflow. The proprietary dye offers highly efficient labeling (Figure 2), very low background (Figure 3), and is compatible with a wide range of EV isolation techniques (Figure 4).

ExoGlow-NTA-labeled liposomes deliver consistent NanoSight NTA data whether in light scattering or fluorescent mode

 

Figure 2. ExoGlow-NTA-labeled liposomes deliver consistent NTA data whether in light scattering or fluorescent mode. The high concordance of NTA and fluorescent NTA data collected from the ExoGlow-NTA Kit internal standards (ExoGlow-NTA-labeled synthetic liposomes) demonstrates the labeling efficiency of the ExoGlow-NTA Dye and accuracy of the fluorescent NTA method for characterizing EVs.

 

ExoGlow-NTA exhibits undetectable background signal

 

Figure 3. ExoGlow-NTA exhibits undetectable background signal. Conventional NTA and Fluorescent NTA of ExoGlow-NTA dye in the absence of EVs shows bias-free undetectable autofluorescence, based on (A) particle counts and (B) imaging.

 

 

Figure 4. ExoGlow-NTA demonstrates that conventional NTA overestimates EV concentration in samples irrespective of EV isolation method. Representative data comparing conventional NTA and fluorescent NTA for EVs isolated using (A) ExoQuick (10 μg serum protein), (B) ultracentrifugation and wash (1 μg serum protein), or (C) column based (1 μg serum protein), shows how much of the conventional NTA signal is due to non-EV particles.

 

Exosome Isolation & Manufacturing

Overview

Accelerate discovery and development with SBI’s Exosome Isolation & Manufacturing Services

Because we know exosomes and we know how to deliver on end-to-end service

 

 

 

 

 

Need exosomes? SBI’s Exosome Isolation & Manufacturing Services can deliver. We’ve been working with exosomes for years, and can reliably and reproducibly isolate exosomes from almost any biofluid—from plasma and tissue culture media to CSF, synovial fluid, and even mouse bronchial alveolar fluid (a challenging sample where exosomes are concerned!). Choose your scale and choose your biofluid, and choose your isolation method. If you have any questions, our experienced services team can provide a customized scientific consultation.

 

How it works

Ordering Exosome Isolation & Manufacturing Services is easy

 

Step 1: Contact us for a quote.

Step 2: Send us your biofluid.

Step 3: Receive your isolated exosomes complete with NanoSight™ characterization

SBI’s Exosome Isolation & Manufacturing Services take advantage of our robust and reliable reagents for exosome isolation.

 

Supporting data

Visit any of our Exosome Isolation pages to see the isolation data, or contact our services team to learn more.

Exosome Engineering

Overview

Are you ready to put exosomes to work? Let SBI help!

Because we know exosomes and we know how to deliver on end-to-end service

 

 

 

 

 

Used by cells to transport specific cargoes of active biomolecules, exosomes can be a powerful tool for labeling cells, delivering therapeutics, studying biological processes, and other applications. But you first need to load your cargo—whether protein, miRNA, or small molecules—and/or encode cell type specificity. Which is where SBI’s Exosome Engineering Services can help. Provided by the experienced team that makes SBI’s Exosome Engineering products, our scientists are ready to produce custom-engineered exosomes for you and can provide as much support as you need, including scientific consultation about engineering methods and exosome design.

 

What kind of engineering can SBI do?

SBI can use one of more of our Exosome Engineering Products to create customized exosomes for you:

  • Label exosomes
    • General protein labeling
    • General RNA labeling
    • Specific protein or RNA labeling
  • Load cargo into exosomes
    • Proteins
    • Nucleic acids, including mRNA, miRNA, lncRNA, DNA
    • Small molecules
  • Tag exosomes for uptake by specific cell types

Don’t see what you need or not sure what would be best for your application? Contact the services team to set up an in-depth phone consultation.

Why choose SBI for engineering exosomes?

Because we’ve been working with exosomes for years. Not only can we reliably and reproducibly isolate exosomes from almost any biofluid, we’ve got the experience to understand the little experimental details, such as cargo loading efficiency, cell uptake efficiency, and more.

 

How it works

Ordering Exosome Engineering Services is easy

 

Step 1: Contact us for a quote.

Step 2:  Send us your biofluid (if applicable)

Step 3:  Receive your engineered exosomes per specifications in the quote

SBI’s Exosome Engineering Services take advantage of our robust and reliable reagents for exosome isolation

 

Supporting data

Visit any of our Exosome Isolation pages to see the isolation data, or contact our services team to learn more.

When you want a partner that will work hard to ensure successful genome engineering, whether it’s HR donor design or complete cell line engineering, choose SBI

From start to finish, SBI’s CRISPR/Cas9 Services can cover all or just part of your entire genome engineering workflow. Our experienced staff understands the intricacies of using Cas9 and know how to overcome many of the common pitfalls that can make genome engineering with CRISPR/Cas9 technology challenging. And because we understand that each project is different, we customize our offering to meet your specific project needs and provide experienced scientific support every step of the way. Choose SBI’s CRISPR/Cas9 Services for successful genome engineering.

If you don’t see the CRISPR/Cas9 service you need below, don’t hesitate to contact us with your project needs—our scientific team is happy to develop and deliver custom genome engineering projects.

CRISPR/Cas9 gRNA and HR Donor Cloning

Overview

Jumpstart your genome engineering projects with SBI’s gRNA and HR Donor Cloning Services

While CRISPR/Cas9 technology greatly simplifies genome engineering, successful execution can be surprisingly challenging. An important first step is the careful design and construction of guide RNAs (gRNAs) and homologous recombination (HR) donors. Save yourself time and trouble and have SBI handle this first step (or have SBI complete your entire genome engineering project for you). Our experienced staff understands the intricacies of using Cas9 and has successfully completed dozens of genome engineering projects.

 

Why choose SBI’s CRISPR/Cas9 genome engineering services?

  • Choose either gRNA and HR donor design and construction or our complete Cell Line Engineering Service
  • Leverage our well-designed CRISPR/Cas9 products which are used in a number of peer-reviewed papers
  • Accomplish more with turn-around times of as little as 1–2 weeks for gRNA cloning, 3–4 weeks for HR donor cloning, and 10–14 weeks for full cell line engineering projects
  • Rest easy with your project in the hands of our experienced team that’s successfully completed dozens of genome engineering projects
  • Enjoy consistent quality, confidentiality, and on-time delivery with all projects completed on-site in our Palo Alto, CA, facility

Not sure what you need or where to begin? Contact the services team to set up a complimentary phone consultation.

 

How It Works

Ordering CRISPR/Cas9 Services is easy

 

Step 1. Contact us for a quote.

Step 2. Send us any necessary materials such as plasmids (optional).

Step 3. Receive your constructs in as little as 1–2 weeks for gRNA plasmids, and 3–4 weeks for HR donor plasmids

For gRNAs, SBI can take care of designing and cloning a single gRNA or multiplex gRNAs against a target locus using any SBI SmartNuclease or SmartNickase vector (or customer provided gRNA cloning vector).

For HR donors, SBI can design and clone homology arms into any SBI HR donor plasmid for knock-out, knock-in, tagging, or single nucleotide modification genome engineering projects. When a custom HR donor plasmid is ordered together with custom gRNA design and cloning, the donor vector will not contain the complete gRNA sequences in the homology arms to ensure full compatibility with the gRNA to be used.

Note that SBI’s CRISPR/Cas9 Genome Engineering Services take advantage of our robust and reliable CRISPR/Cas9 products.

 

Supporting Data

Successful genome engineering with SBI

Successful genome engineering with SBI—effective, efficient knockout of miR-21 in HEK293 cells. gRNA, HR Donor design (with Puro and GFP selection markers), implementation, and analysis performed by SBI’s genome engineering services team. (A) Low relative levels of miR-21—as measured by qPCR in GFP-positive clones—demonstrate the effectiveness of the approach. (B) After excision with Cre recombinase, the inserted GFP and Puro markers are efficiently excised, leaving only a single LoxP site from the HR Donor. From Ho, TT, et al. Targeting noncoding RNAs with the CRISPR/Cas9 system in human cell lines. Nucleic Acids Res. 2015 Feb 18; 43(3):e17. PMCID: PMC4330338.

CRISPR/Cas9 Cell Line Engineering

OVERVIEW:

Go directly to engineered cell lines with SBI’s CRISPR/Cas9 Cell Line Engineering Services

 

 

 

 

 

While CRISPR/Cas9 technology greatly simplifies genome engineering, successful execution can be surprisingly challenging. Save yourself time and hassle and have SBI handle all aspects of your genome engineering workflow, from start to finish. Our experienced staff understands the intricacies of using Cas9 and know how to overcome many of the common pitfalls that can make genome engineering with CRISPR/Cas9 technology challenging. And, because each project is different, we can customize our offering to meet your specific project needs.

 

Why choose SBI’s CRISPR/Cas9 genome engineering services?

  • Choose either gRNA and HR donor design and construction or our complete Cell Line Engineering Service
  • Leverage our well-designed CRISPR/Cas9 products which are used in a number of peer-reviewed papers
  • Accomplish more with turn-around times of as little as 1–2 weeks for gRNA cloning, 3–4 weeks for HR donor cloning, and 10–14 weeks for full cell line engineering projects
  • Rest easy with your project in the hands of our experienced team that’s successfully completed dozens of genome engineering projects
  • Enjoy consistent quality, confidentiality, and on-time delivery with all projects completed on-site in our Palo Alto, CA, facility

Not sure what you need or where to begin? Contact the services team to set up a complimentary phone consultation.

 

How it works

Ordering CRISPR/Cas9 Services is easy

 

Step 1. Contact us for a quote.

Step 2. Send us any necessary materials such as plasmids or cell lines (optional).

Step 3. Receive your engineered cell line in as little as 10–14 weeks.

SBI will use custom Cas9/gRNA constructs and plasmid or oligonucleotide-based HR donor templates to engineer target cell lines for knock-out, knock-in, tagging, or single nucleotide modification applications. SBI can screen cells to identify a clonal line with the desired modification or deliver a mixed population of cells for further characterization.

Note that SBI’s CRISPR/Cas9 Genome Engineering Services take advantage of our robust and reliable CRISPR/Cas9 products.

 

Supporting data

Successful genome engineering with SBI

 

CRISPR/Successful genome engineering with SBI—effective, efficient knockout of miR-21 in HEK293 cells

Successful genome engineering with SBI—effective, efficient knockout of miR-21 in HEK293 cells. gRNA, HR Donor design (with Puro and GFP selection markers), implementation, and analysis performed by SBI’s genome engineering services team. (A) Low relative levels of miR-21—as measured by qPCR in GFP-positive clones—demonstrate the effectiveness of the approach. (B) After excision with Cre recombinase, the inserted GFP and Puro markers are efficiently excised, leaving only a single LoxP site from the HR Donor. From Ho, TT, et al. Targeting noncoding RNAs with the CRISPR/Cas9 system in human cell lines. Nucleic Acids Res. 2015 Feb 18; 43(3):e17. PMCID: PMC4330338.

Affordable and easy custom plasmid construction—choose any SBI vector, send us your sequence, and get your custom construct in as little as 2 weeks

Overview

A simpler, easier way to get the constructs you need

Plasmid Construction Service Vectors

 

 

 

 

 

With SBI’s highly affordable Syn2Clone service, you can order any of SBI’s well-validated and highly published vectors with your gene-of-interest already in it. For little more than the cost of the empty vector, our plasmid construction service will synthesize your gene and clone it directly into the SBI vector of your choice, satisfaction guaranteed. Just send us your sequence and vector selection, and we’ll take care of the rest. If you’re not happy with our plasmid construction service, contact us within thirty days of receiving your Syn2Clone construct and we’ll give you 50% product credit.

Get constructs the easy way with Syn2Clone— contact us with any questions.

  • Fast—custom gene synthesis and sub-cloning in as little as 2 weeks
  • Affordable—custom constructs using any of SBI’s well-validated vectors, such as pCDH lentivectors, AAV, piggyBac, Minicircle, PhiC31 integrase vectors, and more
  • Easy—just send us your sequence, we’ll take care of synthesis and cloning
  • Guaranteed—receive 50% product credit if you’re unhappy with your Syn2Clone construct for any reason (request must be received within 30 days of receiving the shipment)

Here are two examples of recently completed Syn2Clone projects

  • Design, synthesis, and cloning of a complex miR precursor in 2 weeks
  • Design, synthesis, and cloning of a large ORF + reporter fusion, marker, and selection cassette (~8kb single-piece insert) within 7–8 weeks

Choose from any of SBI’s gene delivery platforms

How it works

Ordering Syn2Clone Custom Constructs is easyStep 1: Contact us for a quote. Be sure to include information on your gene-of-interest

Step 2: Receive your custom construct in as little as two weeks

Get high-titer, transduction-ready lentivirus backed by expert technical support, flexible production scales, and different titer options

Overview

Get high-titer, transduction-ready virus

High Quality Lentivirus Packaging Service

 

 

 

 

 

When it comes to cell line engineering with lentivirus, getting high-quality, high-titer lentivirus preparations is often critical for success, especially with hard-to-transduce cell lines. And when it comes to getting high-quality, high-titer lentivirus, you can reliably turn to the Lentivirus Packaging team at SBI.

  • Transduction-ready virus
  • Highly experienced team delivers high-quality packaged virus
  • Consistent and reliable titers delivered at or above specified titer levels
  • Three different titer levels and flexible production scales
  • Trusted OEM lentivirus manufacturer for large life science reagent companies
  • Expert technical support, full confidentiality, and on-time delivery with all projects completed on-site in our Palo Alto, CA, facility

Why turn to SBI for lentivirus packaging?

The answer is our experienced service, high-quality packaged virus, and expert support. We’ve been in the lentiviral packaging business for over a decade—far longer than many companies on the market—and are the trusted OEM partner for lentiviral packaging for several large life science reagent companies. Because all aspects of this service are completed on-site in our Palo Alto, CA, facility, we can offer complete confidentiality, transparency on project status, and fully-informed, expert technical support.

How long does the service take? Is my project too big (or too small)?

Get ready-to-transduce, high-quality, high titer lentiviral preparations at the production scale your project needs—even at large scales of up to 10 mL. Use your own lentivector construct or take advantage of our Syn2Clone or Custom Construct services and we’ll handle vector construction as well. We even have an ultra-high titer offering for demanding applications such as in vivo and stem cell transductions.

Available titer options

If you have any questions about our Lentivirus Packaging Services or don’t see the service you’re looking for, contact us—our scientists are here to help.

 

Get a Quote

Ordering Lentivirus Packaging Services is easy

 

Step 1: Contact us for a quote.

Step 2:  Send us your lentivirus construct and information about the vector, or have us make the vector for you with our Syn2Clone Custom Constructs services.

Step 3: Receive transduction-ready lentivirus

 

Supporting data

Lentivirus produced by SBI exhibits broad tropism and robust in vivo transduction efficiency

 

SBI’s Lentivirus Packaging Service Exhibits Broad Tropism

 

Figure 1. SBI’s packaged lentivirus exhibits broad tropism.

 

Lentivirus Packaging Service Produced by SBI Delivers High Transduction Efficiency in vivo

 

Figure 2. Lentivirus produced by SBI delivers high transduction efficiency in vivoDAPI and GFP imaging of mouse liver six days after infection with either no virus (top) or miRZip-33 virus with a GFP marker (bottom). Lentivirus was introduced into mice via retroorbital injection at 2 x 109 IFU/mouse. Rayner, KJ, et al. miR-33 Contributes to the Regulation of Cholesterol Homeostasis. Science. 2010 June 18. 328(5985):1570. Supporting Online MaterialPMCID: PMC3114628.

Get custom reporter, overexpression, or other type of cell line fast and hassle-free with integrated or episomal transgene delivery, viral or non-viral vectors

Whatever your custom cell line needs—whether it’s for reporter cells that express GFP, or luciferase for high-throughput assays, overexpression cells to study your protein-of-interest, or customized cells for any research application—SBI’s Stable Cell Line Services are ready to deliver. We have access to a range of technologies and an expert team experienced at working with a variety of cell lines, including adherent and suspension cells. And as with every SBI service, you get our quality customer service experience with high success rates, frequent communication, and fast, reliable timelines.

If you don’t see the cell line service you need below, don’t hesitate to contact us with your project needs—our scientific team is available to deliver customized cell lines for any application.

Custom Overexpression Stable Cell Lines

Overview

Leverage SBI’s viral and non-viral vectors in robust overexpression cell lines

 

 

 

 

 

Whether you want constitutive or inducible overexpression of a protein or regulatory RNA, SBI’s services team can create the construct and the cell line. We have access to a range of technologies—including stably integrating viral and non-viral vectors—and an expert team experienced at working with a variety of cell lines, including adherent and suspension cells. The result is hundreds of successfully constructed stable cell lines for highly satisfied customers.

 

Why choose SBI’s Stable Cell Line Services?

  • Save time by having SBI build your custom cell lines
  • Leverage our highly-regarded gene expression and delivery technologies
  • Accomplish more with our fast turnaround times and end-to-end services
  • Turn to the company that other companies turn to—we are a trusted stable cell line manufacturer for many large pharma/biotech companies
  • Enjoy consistent quality, confidentiality, and on-time delivery with all projects completed on-site in our Palo Alto, CA, facility

Available gene expression and delivery technologies

Viral Systems

  • Lentivectors—high titer and reliable
    • Random integration
    • Limited insert size
    • High copy number
  • Lentivirus Packaging—reliable gene delivery
    • High and ultra-high titers
    • Efficient transduction
    • Optimized reagents

Non-viral Systems

  • PhiC31 Integrase System—one-step single-copy integration
    • Site-specific integration
    • Unlimited insert size
    • Single copy number
  • PinPoint Targeted Integration System—excellent for creating isogenic cell lines
    • Site-specific integration
    • Unlimited insert size
    • Single copy number

Not sure what you need or where to begin? Contact the services team to set up a complimentary phone consultation.

 

How It Works

Ordering Stable Cell Line Services is easy

 

Step 1. Contact us for a quote.

Step 2. Send us any necessary materials such as parent cell lines (optional).

Step 3. Receive your stable cell line (turn-around time depends on the complexity of your project).

SBI’s Stable Cell Line Services are a great way to leverage our highly-regarded lentivector, PhiC31 Integrase, and PinPoint Targeted Integration technologies.

 

Supporting Data

Successful generation of stable cell lines at SBI

 

Example of data generated using cell lines constructed by SBI

 

 

 

 

 

 

 

 

 

Example of data generated using cell lines constructed by SBI. (Top row) Stable cell line expressing two genes-of-interest with GFP, RFP, hygromycin, and puromycin selection markers. H2286 adherent cells were grown under puromycin and hygromycin selection. (Bottom row) Stable cell line expressing two separate genes-of-interest with GFP, RFP, hygromycin, and puromycin selection markers. H929 suspension cells were grown under puromycin and hygromycin selection.

Custom Luciferase-labeled Stable Cell Lines

Overview

Get greater insights from in vivo studies with luciferase-labeled cell lines

 

 

 

 

 

When you need custom luciferase-labeled cell lines, SBI’s Custom Luciferase-labeled Stable Cell Line Services can save you the time and hassle of making the cell lines yourself. We have access to a range of technologies and an expert team experienced at working with a variety of cell lines, including adherent and suspension cells. The result is hundreds of successfully constructed stable cell lines and highly satisfied customers.

 

Why choose SBI’s Stable Cell Line Services?

  • Save time by having SBI build your custom cell lines
  • Leverage our highly-regarded gene expression and delivery technologies
  • Accomplish more with our fast turnaround times and end-to-end services
  • Turn to the company that other companies turn to—we are a trusted stable cell line manufacturer for many large pharma/biotech companies
  • Enjoy consistent quality, confidentiality, and on-time delivery with all projects completed on-site in our Palo Alto, CA, facility

Available gene expression and delivery technologies

Viral Systems

  • Lentivectors—high titer and reliable
    • Random integration
    • Limited insert size
    • High copy number
  • Lentivirus Packaging—reliable gene delivery
    • High and ultra-high titers
    • Efficient transduction
    • Optimized reagents

Non-viral Systems

  • PhiC31 Integrase System—one-step single-copy integration
    • Site-specific integration
    • Unlimited insert size
    • Single copy number
  • PinPoint Targeted Integration System—excellent for creating isogenic cell lines
    • Site-specific integration
    • Unlimited insert size
    • Single copy number

Not sure what you need or where to begin? Contact us.

 

How It Works

Ordering Stable Cell Line Services is easy

 

Step 1. Contact the services team for a quote by emailing services@systembio.com.

Step 2. Send us any necessary materials such as parent cell lines (optional).

Step 3. Receive your stable cell line (turn-around time depends on the complexity of your project).

SBI’s Stable Cell Line Services are a great way to leverage our highly-regarded lentivector, PhiC31 Integrase, and PinPoint Targeted Integration technologies.

 

Supporting Data

Successful generation of dual luciferase and GFP-labeled NF-κB cell line

"Example

Example of data generated using a dual GFP and luciferase-labeled cell line constructed by SBI Monitor and quantitatively measure NF-κB transactivation in live cells

 

Figure 1. Example of data generated using a dual GFP and luciferase-labeled cell line constructed by SBI—monitor and quantitatively measure NFκB transactivation in live cells. Monitor NF-κB activation in real time with GFP using SBI’s NF-κB/293/GFP-Luc Transcriptional Reporter Cell Line (Cat.# TR860A-1).

 

Example of data generated using a dual GFP and luciferase-labeled cell line constructed by SBI Monitor and quantitatively measure NF-κB transactivation in live cells

Figure 2. Example of data generated using a dual GFP and luciferase-labeled cell line constructed by SBI—monitor and quantitatively measure NFκB transactivation in live cells. Quantitatively measure NF-κB activation in real time with luciferase using SBI’s NF-κB/293/GFP-Luc Transcriptional Reporter Cell Line (Cat.# TR860A-1).

Reporter Cell Lines

Overview

Get greater insights from in vivo studies with luciferase-labeled cell lines

 

 

 

 

 

While SBI already offers a number of ready-to-go reporter cell lines, everyone’s studies are different. Which is why SBI also offers Custom Stable Reporter Cell Line Services that can generate promoter- and/or enhancer-reporter fusions. Save time and effort while leveraging our comprehensive range of technologies and our expert team which has years of experience working with a variety of cell lines, including adherent and suspension cells.

 

Why choose SBI’s Stable Cell Line Services?

  • Save time by having SBI build your custom cell lines
  • Leverage our highly-regarded gene expression and delivery technologies
  • Accomplish more with our fast turnaround times and end-to-end services
  • Turn to the company that other companies turn to—we are a trusted stable cell line manufacturer for many large pharma/biotech companies
  • Enjoy consistent quality, confidentiality, and on-time delivery with all projects completed on-site in our Palo Alto, CA, facility

Available gene expression and delivery technologies

Viral Systems

  • Lentivectors—high titer and reliable
    • Random integration
    • Limited insert size
    • High copy number
  • Lentivirus Packaging—reliable gene delivery
    • High and ultra-high titers
    • Efficient transduction
    • Optimized reagents

Non-viral Systems

  • PhiC31 Integrase System—one-step single-copy integration
    • Site-specific integration
    • Unlimited insert size
    • Single copy number
  • PinPoint Targeted Integration System—excellent for creating isogenic cell lines
    • Site-specific integration
    • Unlimited insert size
    • Single copy number

Not sure what you need or where to begin? Contact the services team to set up a complimentary phone consultation.

 

How It Works

Ordering Stable Cell Line Services is easy

 

Step 1. Contact us for a quote.

Step 2. Send us any necessary materials such as parent cell lines (optional).

Step 3. Receive your stable cell line (turn-around time depends on the complexity of your project).

SBI’s Stable Cell Line Services are a great way to leverage our highly-regarded lentivector, PhiC31 Integrase, and PinPoint Targeted Integration technologies.

 

Supporting Data

Successful generation of dual luciferase and GFP-labeled NF-κB cell line

 

SBI Website Content/04_Services Pages/Z_Services Image Files/GFP_induce.jpg, Example of data generated using a dual GFP and luciferase-labeled cell line constructed by SBI Monitor and quantitatively measure NF-κB transactivation in live cells]

"Example

 

Example of data generated using a dual GFP and luciferase-labeled cell line constructed by SBI Monitor and quantitatively measure NF-κB transactivation in live cells” />

 

Figure 1. Example of data generated using a dual GFP and luciferase-labeled cell line constructed by SBI—monitor and quantitatively measure NFκB transactivation in live cells. Monitor NF-κB activation in real time with GFP using SBI’s NF-κB/293/GFP-Luc™ Transcriptional Reporter Cell Line (Cat.# TR860A-1).

 

Example of data generated using a dual GFP and luciferase-labeled cell line constructed by SBI Monitor and quantitatively measure NF-κB transactivation in live cells

 

Figure 2. Example of data generated using a dual GFP and luciferase-labeled cell line constructed by SBI—monitor and quantitatively measure NFκB transactivation in live cells. Quantitatively measure NF-κB activation in real time with luciferase using SBI’s NF-κB/293/GFP-Luc Transcriptional Reporter Cell Line (Cat.# TR860A-1).

Let SBI’s Custom Minicircle Services team handle minicircle cloning and production for easy access to the technology—get sustained transgene expression free from foreign DNA

Overview

All the benefits of minicircle technology without the hassles

Custom Minicircle Services Available to you

 

 

 

 

 

When you want sustained transgene expression without the risks of introducing exogenous DNA—such as for developing animal models or pioneering new gene therapy approaches—minicircle technology is a great option. But many labs don’t have either the time to spend constructing and producing minicircles or the in-house expertise. Which is why SBI offers Custom Minicircle Services.

Delivered by our experienced scientific team, our Minicircle Services are highly flexible and can include a complete end-to-end experience—we clone your cDNA, miRNA, or shRNA into one of our highly-regarded minicircle parental vectors and produce large quantities of minicircles—or only parts of the cloning and production process. We can even customize the parental vector for you.

Whether you need 250 µg or 10 mg of minicircles, we can deliver, with a typical end-to-end project taking as little as four weeks.

  • Access Minicircle Technology for research use with no licensing restrictions when ordering ready-to-use minicircle DNA through our production service
  • Leverage the minicircle platform for durable gene expression in your most demanding applications
  • Rest easy with your project in the hands of a team that routinely produces minicircles for large pharma partners

And, as with all of SBI’s Custom Services, we offer a high-quality, full service experience:

  • Dedicated scientific project managers ready to answer any of your questions, whether they’re on the science or the timelines
  • Full transparency and frequent communications about your project
  • Complete confidentiality, consistent quality, and reliable timelines that are made possible by having all aspects of your project conducted on-site in our Palo Alto, California facility

If you have any questions about our Custom Minicircle Services or would like a quote, contact us—our scientists are here to help.

How it works

Ordering Minicircle Services is easy

 

Step 1. Contact us for a quote.

Step 2. Send us your producer plasmid, or have us make the vector for you.

Step 3. Receive ready-to-transfect minicircle plasmids in as little as four weeks.

What are minicircles?

Produced as small circular DNA fragments after excision from a parental plasmid, the non-viral, episomal minicircle expression cassette is free of any bacterial plasmid DNA sequences that can lead to silencing, and comes with a variety of promoter and reporter combinations. Their small size facilitates more efficient transfection than what’s possible with standard-sized plasmids, and, while minicircles do not replicate with the host cell, expression lasts for 14 days or longer in dividing cells, and can continue for months in non-dividing cells.

Custom Minicircle Services Method

 

Supporting data

Achieve sustained expression after transfection in vitro and in vivo

 

Transfection of 1 µg of Minicircle Possible with our Custom Minicircle Services

 

Figure 1. Easy, sustained transfection in most cell types (left) and transgene expression in animal models for weeks (right). Transfection of 1 µg of minicircle DNA (pMC.CMV-MCS-EF1-GFP-SV40PolyA, Cat.# MN511A-1) into HEK293 cells delivers over one week of robust gene expression.  Hydrodynamic tail vein injection of minicircle DNA (CMV-GFP-Luc) into mice shows excellent expression after several weeks. Minicircle-delivered transgenes retain robust expression that can last for weeks compared to transgenes that are delivered using plasmid DNA where expression is rapidly lost. In this study, 40 µg of minicircle DNA was introduced into mice via hydrodynamic tail vein injection.

SBI’s services team is ready to support your RNA studies with custom RNA synthesis, qPCR-based profiling, and custom miRNA overexpression and knockdown constructs

Whether you want to get fast protein expression by transfecting or injecting mRNA into cells or are studying regulatory RNAs—miRNAs, lncRNAs, etc—through profiling, overexpression, or knockdowns, the SBI team is ready to support your studies with our custom RNA synthesis services. Backed by our high-quality RNA products, including the most comprehensive miRNA libraries for qPCR and extensive, pre-built miRNA precursor and miRNA knockdown constructs, our team delivers an excellent customer service experience. We deliver high success rates, frequent customer communication, and fast, reliable timelines. Plus the confidentiality that comes with having all aspects of the project completed on-site in our Palo Alto, CA facility.

If you don’t see the RNA service you need below, don’t hesitate to contact us with your project needs—our scientific team is available to deliver custom RNA services for many applications.

mRNA Synthesis Services

When you want in vitro-transcribed mRNAs for immediate protein expression or other applications, turn to SBI’s mRNA Synthesis Services

Overview

Efficiently generate mRNAs

 

 

 

 

 

When you want instant expression after transfection into cells, deliver your gene-of-interest as an mRNA made with SBI’s mRNAExpress mRNA Synthesis Kit. Designed to generate in vitro transcripts for transfection of mammalian cells, micro-injection into oocytes, in vitro translation, and other related applications, this high-yield kit can produce 20—40 µg of high-quality mRNAs in one standard reaction, and comes with a number of performance-enhancing features:

  • Leverages the robust T7 RNA Polymerase
  • Incorporates an anti-reverse cap analog (ARCA) into the transcript to increase translation efficiency
  • Reduces host cell immune response and enhances stability by incorporating modified nucleotides (5-Methylcytidine-5′-Triphosphate and Pseudouridine-5′-Triphosphate) and a poly-A tail
  • Degrades the DNA template after RNA synthesis with the included DNase
  • Removes the 5′ triphosphates at the end of the RNA with the included phosphatase to further reduce innate immune responses in mammalian cells
  • Employs a robust clean-up spin column system that delivers high yields of mRNAs that are ready for most downstream applications

The Kit includes the following reagents that are critical in vitro transcription:

  • pMRNAXP mRNAExpress Vector so you can clone and then transcribe your cDNA-of-interest
  • Sequencing primers to confirm the identity of the cDNA insert cloned into the pMRNAXP Vector
  • Tail PCR primer mix to add a poly-A tail to the DNA template
  • In vitro transcription reagents—T7 RNA polymerase, in vitro transcription buffer, NTP/Cap mix, RNase-free DNase, phosphatase buffer and RNase-free phosphatase
  • Clean-up spin column system, including binding, wash, and elution buffers, collection tubes, and collection tubes with filter cartridges

How it works

The mRNAExpress mRNA Synthesis Kit workflow

 

 The mRNAExpress mRNA Synthesis Kit has a straightforward workflow:

  1. Clone the cDNA of your gene-of-interest in the pMRNAXP mRNAExpress Vector (included in the Kit, but also available separately, # MR000PA-1)
  2. Tail-PCR amplify the in vitro transcription template from the pMRNAXP Vector with your insert cloned in. The tail PCR reaction adds a poly-A tail to the DNA template. Purify the template using a spin column.
  3. Perform the in vitro transcription reaction (uses the ARCA and modified nucleotides)
  4. Remove the template DNA and the terminal 5′ triphosphates with the included DNase and phosphatase.
  5. Prepare the mRNA transcripts for transfection with the included clean-up spin column system.
  6. Measure the mRNA concentration and you are ready for transfection.

 

Supporting data

Get instant expression when you deliver mRNAs made with the mRNAExpress Vector mRNA Synthesis Kit

 

Strong GFP and RFP expression after transfection with mRNAs made with the mRNAExpress Vector mRNA Synthesis Kit

Figure 1. Strong GFP and RFP expression after transfection with mRNAs made with the mRNAExpress Vector mRNA Synthesis Kit. GFP and RFP mRNAs generated using the mRNAExpress mRNA Synthesis Kit were transfected into human foreskin fibroblasts (HFFs) using 1 µg of each mRNA per 24-well plate of cells.

miRNA & lncRNA Profiling Services

Sensitive and highly quantitative qPCR-based miRNA or lncRNA profiling from human, mouse, rat, or other model system samples with as little as 10 pg of RNA

Overview

Confident quantitation of miRNAs and lncRNAs

 

 

 

 

 

Leveraging our sensitive and highly quantitative RNA products, SBI’s miRNA and lncRNA Profiling Services deliver comprehensive, end-to-end profiling services that can be customized to meet your project’s needs. Have us use one of our pre-made qPCR Arrays, such as our genome-wide miRNome Profilers, or choose your own miRs for a custom array. Because we can take advantage of the sensitivity of our QuantiMir technology, we can profile as little as 10 pg of miRNA or lncRNA. And the service even includes delivery of fully analyzed data and scientific consultation with our experienced staff. Backed by our high-quality RNA products, we deliver high success rates, frequent customer communication, and fast, reliable timelines.

 

Why choose SBI’s RNA Services?

  • Stay focused on your science by having SBI handle qPCR profiling of your miRNAs and lncRNAs
  • Effortlessly leverage our sensitive QuantiMir technology and robust qPCR Arrays
  • Accomplish more with turn-around times of approximately 2 weeks and comprehensive end-to-end services
  • Enjoy consistent quality, confidentiality, and on-time delivery with all projects completed on-site in our Palo Alto, CA, facility

Not sure what you need or where to begin? Contact the services team to set up a complimentary phone consultation.

Available Pre-Made Arrays (click on the catalog number to get details on the array)

CATALOG # PRODUCT SPECIES
miRNA Arrays
RA610A-1 Cancer MicroRNA “OncoMir” qPCR Array Human
RA620A-1 Stem Cell MicroRNA Profiling Panel Human
RA660A-1 hsa-miRNome MicroRNA Profiling Kit Human
RA670A-1 mmu-miRNome MicroRNA Profiling Kit Mouse
RA680B-1 rno-miRNome MicroRNA Profiling Kit Rat
Exosomal miRNA Arrays
RA810A-1 Human SeraMir Exosome RNA 384 microRNA qPCR Profiler Assay Set Human
RA811A-1 Mouse SeraMir Exosome RNA 384 microRNA qPCR Profiler Assay Set Mouse
RA812A-1 Rat SeraMir Exosome RNA 384 microRNA qPCR Profiler Assay Set Rat
lncRNA Arrays
RA900A-1 LncRNA Profiler qPCR Array Kit Human
RA930A-1 Mouse LncRNA Profiler qPCR Array Kit Mouse
RA950A-1 Regulatory RNA qPCR Profiler Human

 

How It Works

Ordering RNA Services is easy

 

Step 1: Contact us for a quote.

Step 2: Ship your total RNA samples frozen in 10 µl water on dry ice (Trizol-extracted RNA works great). As little at 500ng will be enough.

Step 3: SBI will generate miRNA-derived or lncRNA-derived cDNA suitable for qPCR and profile the RNAs using the qPCR array of your choice.

Step 4: All raw Ct data will be emailed directly to you.

Step 5: SBI scientists will perform statistical cross-comparison expression analysis and email this to you.

Step 6: SBI will contact you to review your data via a conference call.

Custom miRNA Precursor and anti-miRNA (miRZip) Services

Get exactly the tools you need to study miRNA overexpression or knockdown with our custom miRNA precursor and anti-miRNA (miRZip) construction services

Overview

Get exactly the tools you need to study miRNA functions

 

 

 

 

 

While SBI already offers a comprehensive library of miRNA precursors for overexpressing miRNAs and miRZip anti-miRNA constructs for knocking down miRNA levels, you might not find the right construct for your project. Which is why SBI offers custom services to create exactly the miRNA precursor or anti-miRNA you need, made by the same scientists that make our pre-built constructs. Your custom construct can be prepared as high-quality plasmid DNA as packaged, ready-for-transduction lentivirus. Backed by our high-quality RNA products and extensive lentivirus and miRNA expertise, we deliver high success rates, frequent customer communication, and fast, reliable timelines.

 

Why choose SBI’s RNA Services?

  • Stay focused on your science by having SBI handle construction of your miRNA precursors or anti-miRNA miRZip constructs
  • Effortlessly leverage our extensive experience with both lentivirus and miRNA technologies
  • Accomplish more with fast turn-around times and comprehensive end-to-end services
  • Enjoy consistent quality, confidentiality, and on-time delivery with all projects completed on-site in our Palo Alto, CA, facility

Not sure what you need or where to begin? Contact the services team to set up a complimentary phone consultation.

 

How it Works

Ordering RNA Services is easy

 

  • Contact us for a quote.
  • Send us any necessary materials such as miRNA sequence
  • Receive your constructs as plasmids or already packaged into ready-to-transduce lentivirus.

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