SignalChem - Stratech

SignalChem is a biotechnology company focused on the research, development and production of innovative cell signaling products to advance basic research and drug discovery efforts, with specific emphasis on the production of highly purified, biologically active, human recombinant proteins.

Cellular signalling or the mechanism by which a cell transmits its signals inwards, ultimately producing a specific biological response, is of fundamental clinical importance. It is well documented that aberrations in these discrete pathways have been linked to the development of a variety of human diseases including cancer, cardiovascular disease, metabolic disorders, neurodegenerative disease and inflammatory conditions amongst others.

With the success of signal transduction inhibitors in the clinic, there is an intensive effort to identify targets linked with the pathogenesis of a human disease and to develop selective molecules to perturb these targets in an effort to improve patient prognosis and minimise the non-specific systemic side-effects associated with generalised treatment modalities.

The founders and scientific members of SignalChem have the knowledge to understand the architecture and biology of these discrete signalling pathways and to leverage this expertise to bring value-added products to the market. SignalChem is emerging as a leader and a key contender in the life science recombinant protein market place.

SignalChem’s core product areas include active human protein kinases, active human protein phosphatases, unactive signalling proteins, assay kits and biologically relevant kinase and phosphatase substrates.

Custom Services

Discover how SignalChem’s Custom Services can make a difference in your research.

Our scientists understand how pivotal cell signaling proteins are for every research/drug discovery project to be successful, and we keep this principle in mind when collaborating with scientists wanting to procure fully functional, biologically active, custom made recombinant proteins serving their individual research needs.

Find out more below;

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COMPOUND PROFILING

SCREENING HIGHLIGHTS

SignalChem’s compound selectivity profiling service determines the respective inhibitory profile and the putative mechanism of action. All compounds are profiled against a panel of targets either using an individual dose, a single concentration or at multiple concentrations in order to allow in-depth IC50 determinations. In addition, the protein kinase assays can be performed under varying ATP concentrations to evaluate the competitive effects of ATP.

The compound selectivity profiling service offered by SignalChem is a very economical and convenient approach to the drug discovery continuum with a two-week turnaround of your specific profiling results. Compounds can be supplied by the client as DMSO stocks of known concentration, as solid material in vials.

Figure 1: Compound Selectivity Profiling Against 48 Targets available as part of SignalChem’s Compound Profiling Service.

Figure 2: The IC50 value of a compound was determined by plotting the log of the concentration of the compound vs. the % inhibition of the kinase.

Restrictions

JAK2*: Patent restrictions on this product exclude it as a profiling target for US customers.

Confidentiality

SignalChem maintains all information under the strictest confidentiality. All information and/or materials supplied by the customer will be used as directed by the client. Upon completion of the project, all materials will be either returned to the client or disposed of accordingly. SignalChem is willing to execute confidentiality agreements with its clients.

CUSTOM PROTEIN DEVELOPMENT

SignalChem scientists have gained extensive experience in manufacturing highly functional cellular signaling proteins including protein kinases, protein phosphatases and cellular protein substrates, among others.

Depending upon the client’s needs, SignalChem will work with you to custom develop the target to your desired specifications within a realistic and suitable timeframe. Our products have successfully been used in various applications including high-throughput screening, activity assays, binding assays, cell-based assays, protein-protein interaction studies, structural studies and more.

OUR CUSTOM PROTEIN DEVELOPMENT CAPABILITIES

Production of the full-length or truncated form of the desired targets
Production of highly purified proteins in desired final formulation
Production of the target as either untagged or tagged proteins
Production of the targets with high biological activity
Production of proteins from μg to mg scale

STEP-WISE STRATEGY FOR DEVELOPMENT OF CUSTOM PROTEINS FOR CLIENTS

Cloning of Target Gene

Based on the target gene, SignalChem scientists will design specific primers against the target of interest and amplify the gene product from cDNA libraries derived from various human cell lines.The cloned target gene is subsequently cloned into the appropriate expression vectors to maximize the expression levels of the desired target.

Expression of Desired Protein

Based upon the respective needs of the client, SignalChem scientists will recommend the most suitable expression system to use and as a part of the custom project. The choice of the cells and the expression systems is governed by the level of post-translational modification required and whether enzymatic activity is essential.

Purification & Additional Modification of Protein

The selected target is purified from the cellular lysate using established protocols to maintain the protein structure and activity. Additional steps will be taken based on the client’s requirements including phosphorylation/dephosphorylation, tag removal, concentration increase, buffer exchange, and more.

Characterization of Target Protein

In the final stages of development, the protein product will be evaluated in multiple tests to ensure the concentration, purity, and activity meet the specifications set by the client.

Confidentiality

CUSTOMIZED RAB PRODUCTION

Recombinant antibodies (rAbs) are highly specific biochemical probes, which are gaining popularity for use in life sciences research, diagnostics and clinical therapy. Unlike traditional monoclonal antibodies (mAbs), rAbs are generated in vitro using synthetic genes, and do not require the use or harm to animals during the production process. Due to continuous interest and high demand, more and more rAb sequences are becoming available to give researchers a growing library of affinity probe designs to choose from.

SignalChem is leveraging our years of expertise to produce high-purity recombinant proteins in various cell systems to provide custom recombinant antibody production services.

SIGNALCHEM’S CUSTOM rAb PRODUCTION SERVICE

SEQUENCE DESIGN

Confidentiality

Enzymes

KINASES

Protein Kinases are a family of pivotal enzymes with 518 members implicated in various cell signaling pathways, constituting over 2% of the human genome. Kinases exert their roles by phosphorylating their respective physiological substrates. They catalyze the transfer of γ-phosphate from ATP or GTP to the hydroxyl groups of serine, threonine and tyrosine residues on the substrate proteins or the glycosyl moieties in lipids. Deregulation of Kinase could lead to the changes in enzymatic activity and/or expression, subcellular localization, the stability of kinase, protein-protein interactions. Aberrant regulation of Kinase-mediated signaling pathways is responsible for all major human pathological conditions such as cancer, cardiovascular disease, neurodegenerative diseases and metabolic disorders.

SignalChem offers a broad range of recombinant protein and lipid Kinases of superior purity and activity to assist the global academic research and drug discovery efforts with the focus on Kinases. Its active Kinase product line is one of the most comprehensive in the world in terms of the coverage and has been utilized by many pharmaceutical companies in their drug discovery programs. An in-house product development team with over 100 years of combined experience in Kinase biochemistry is behind the production and quality control of all the Kinase-related products as well as assay development.

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Mutant Kinases

PHOSPHATASES

Phosphatases

Protein phosphorylation is a reversible post-translational modification carried out by two families of enzymes, Protein Kinases and Protein Phosphatases. The interplay between these two families of enzymes is responsible for modulating the phosphorylation state of their physiological substrates, and thereby their activities, subcellular localization and interactions with other proteins. Similar to Protein Kinases, Protein Phosphatases play pivotal roles in a myriad of biological processes including cell growth, proliferation, survival, and apoptosis. Protein Phosphatases are broadly categorized according to specific amino acids they dephosphorylate into two major families: protein serine/threonine phosphatases, and protein tyrosine phosphatases, which also include the dual-specificity phosphatases.

SignalChem, with comprehensive active Protein Kinase product line, has utilized our expertise in the recombinant protein production to manufacture biologically active phosphatases including PP1s, PP2s, PTPNs, and more. An in-house product development team with over 100 years of combined experience in protein biochemistry is behind the production and quality control of all the phosphatases as well as the method development for their activity assays. We aim to manufacture our phosphatases with superior quality and activity to facilitate and assist the global research and drug discovery efforts aiding human health.

Name	Catalog Number	Species	Tag	Expression System	Sequence	Genbank Number
CDC25A, Active	C04-20G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_001789
CDC25B, Active	C04-20BG	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_021873
CDC25C, Active	C04-20CG	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC019089
DUSP22 (MKPX), Active	D22-20G	Human	GST	E.coli	Full Length	NM_020185
PP1A, Active	P13-20G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_002708
PP1A, Active	P13-20GH	Human	GST	E.coli	Full length	NM_002708
PP1B, Active	P14-20G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_002709
PP2A alpha/PPP2R1A Complex, Active	P16-20BH	Human	HIS	Sf9 insect cells using baculovirus	Full Length	NM_002715
PP2Aα/PPP2R1A Complex, Active	P16-20BG	Human	GST	Sf9 insect cells using baculovirus	Full length	NM_002715
PP2Calpha, Active	P02-20G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_021003
PP2Calpha, Active	P02-20BG	Human	GST	E.coli	Full Length	NM_021003
PP2Cgamma, Active	P07-20G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_177983
PTEN, Active	P23-20G	Human	GST	E.coli	Full Length	NM_000314
PTEN, Active	T03-20H	Human	HIS	Sf9 insect cells using baculovirus	Full length	NM_000314
PTPN1 (PTP1B), Active	P28-20G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_002827
PTPN11 (SHP2), Active	P38-21G	Human	GST	E.coli	246-593	NM_002834
PTPN11 (SHP2), Active	P38-20G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_002834
PTPN11 (T253M Q257L), Active	P38-22G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_002834
PTPN12 (PTP-PEST), Active	P39-21G	Human	GST	E.coli	1-355	NM_002835
PTPN13 (FAP-1), Active	P40-21G	Human	GST	E.coli	2169-2485	NM_080683
PTPN2 (TC-PTP), Active	P29-20G	Human	GST	E.coli	Full Length	NM_080422
PTPN6 (SHP1), Active	P33-20G	Human	GST	E.coli	Full Length	NM_080548
PTPN6 (SHP1), Active	P33-20H	Human	HIS	E.coli	Full Length	NM_0080548
PTPN7 (LC-PTP), Active	P34-20G	Human	GST	E.coli	Full Length	NM_002832
PTPRA (LRP), Active	P48-21G	Human	GST	E.coli	174-802	NM_002836
PTPRB, Active	P49-21G	Human	GST	Sf9 insect cells using baculovirus	1552-end	NM_002837
PTPRC (CD45), Active	P50-21H	Human	HIS	Sf9 insect cells using baculovirus	604-end	NM_002838
PTPRC (CD45), Active	P50-21BG	Mouse	GST	E.coli	592-end	NM_001111316
PTPRE, Active	P52-21G	Human	GST	Sf9 insect cells using baculovirus	75-end	BC050062
PTPRF (LAR), Active	P53-21G	Human	GST	E.coli	1275-1897	NM_002840
PTPRJ (CD148), Active	P56-21G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_002843
PTPRS (PTP- sigma), Active	P64-21G	Human	GST	E.coli	883-1210	NM_130855
SHIP1, Active	P08-20G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_005541
SHIP2, Active	P09-20G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_001567
WIP1, Active	P04-20G	Human	GST	Sf9 insect cells using baculovirus	Full length	BC016480

EPIGENETIC ENZYMES

Epigenetic enzymes are crucial in maintaining key cellular functions by modifying chromatin structures and controlling gene transcription process. These reversible epigenetic DNA and histone modifications are controlled by important epigenetic enzymes such as methyltransferases and acetyltransferases. Dysregulation of these enzymes have been observed in myriad human diseases such as cancer. In recent years, epigenetic enzymes have emerged as some of the most promising classes of targets for drug discovery projects and development projects.

Scientists at SignalChem have developed a full range of epigenetic enzymes including acetyltransferases, histone deacetylases, methyltransferases and more. Our high-quality recombinant epigenetic enzymes and the assay methods are valuable tools for scientists to conduct research in epigenetics and for the pharmaceutical companies to develop novel therapeutics modulating these biological targets.

Other Modifying Enzymes

Name	Catalog Number	Species	Tag	Expression System	Sequence	Genbank Number
ASH2L Protein	A372-30BG	Human	GST	E.coli	Full Length	BC015936
BRD3 Protein	B17-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_007371
DNMT1 Protein	D351-31G	Mouse	GST	Sf9 insect cells using baculovirus	766-end	BC053047
DPY30 Protein	D369-30H	Human	HIS	E.coli	Full Length	NM_032574
EED Protein	E310-30H	Human	HIS	E.coli	Full Length	BC068995
EZH1 Protein	E395-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC015882
EZH2 (Y641C) Protein	E396-32BG	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC010858
EZH2 (Y641H) Protein	E396-32DG	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC010858
EZH2 (Y641N) Protein	E396-32EG	Human	GST	Sf9 insect cells using baculovirus	Full-Length	BC010858
EZH2 (Y641S) Protein	E396-32FG	Human	GST	Sf9 insect cells using baculovirus	Full-Length	BC010858
EZH2 Protein	E396-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC010858
HEMK1 Protein	H311-30G	Human	GST	Sf9 insect cells using baculovirus	Full-Length	NM_016173
KAT3A (CREBBP) Protein	K312-31G	Human	GST	Sf9 insect cells using baculovirus	518-1207	NM_004380
KAT3B (EP300) Protein	K312-31BG	Human	GST	Sf9 insect cells using baculovirus	532-1153; contains the catalytic domain	NM_001429
KAT4 Protein	K313-31G	Human	GST	Sf9 insect cells using baculovirus	1032-end	NM_138923
KAT8 (MYST1) Protein	K317-31G	Human	GST	Sf9 insect cells using baculovirus	2-467	BC037773
KAT9 (ELP3) Protein	K318-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_018091
LCMT2 Protein	L322-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_014793
MEP50 (WDR77) Protein	W329-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC001679
METTL1 Protein	M321-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_005371
METTL11A Protein	M331-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC001396
METTL14 Protein	M334-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_020961
METTL2A Protein	M322-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_181725
METTL3 Protein	M323-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_019852
MGMT Protein	M354-30H	Human	HIS	Sf9 insect cells using baculovirus	Full Length	BC000824
P300 Protein	P07-31G	Human	GST	Sf9 insect cells using baculovirus	1283-1673	NM_001429
PCAF Protein	P09-31G	Human	GST	E.coli	431-end	NM_003884
PCMT1 Protein	P314-30G	Human	GST	Sf9 insect cells using baculovirus	2-end	BC007501
PCNA Protein	P11-30H	Human	HIS	E.coli	Full Length	NM_002592
PRDM2 (KMT8) Protein	P333-31G	Human	GST	Sf9 insect cells using baculovirus	1-340	NM_012231
PRMT2 Protein	P365-30BG	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC000727
PRMT5 Protein	P365-30EG	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_006109
PRMT7 Protein	P365-30GG	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC000146
RBBP4 Protein	R314-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC075836
RBBP5 Protein	R315-30H	Human	HIS	E.coli	Full Length	NM_005057
SETD4 Protein	S353-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC002898
SETD8 Protein	S345-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC050346
SETDB1 (KMT1E) Protein	S346-31G	Human	GST	Sf9 insect cells using baculovirus	715-end	BC028671
SETDB2 Protein	S346-30BG	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC047434
SETMAR Protein	S347-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC011635
TRDMT1 (DNMT2) Protein	T352-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC047733
WDR5 Protein	W325-30H	Human	HIS	E.coli	Full Length	BC001635
WHSC1L1 (NSD3) Protein	N378-31G	Human	GST	Sf9 insect cells using baculovirus	1038-end	BC113469
WTAP Protein	W369-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_004906

UBIQUITIN ENZYMES

Ubiquitin Enzymes and Proteins

Protein modifications by ubiquitin (Ub) or ubiquitin-like proteins (UBLs) participate in many critical cellular processes such as cell-cycle regulation, DNA repair, oncogenesis, antiviral pathways and most notably, proteasomal degradation of target proteins. Ubiquitination and modification by UBLs share a similar catalytic cascade which requires the sequential action of three classes of enzymes: E1 activating enzymes, E2 conjugating enzymes, and E3 ligases. Research has linked dysregulation of the Ub/UBLs modification system to numerous diseases including cancer, immunological disorders, and neurodegeneration. Thus, the high substrate specificity provided by combinations of over 30 E2’s and over 600 E3’s makes these enzymes emerging drug targets.

In response to growing market demand, SignalChem has developed an extensive array of products encompassing ubiquitin proteins and active enzymes, Ub/UBL modifiers, and substrates in the ubiquitination, SUMOylation, ISGylation and NEDDylation processes. Using Promega’s AMP-Glo™ technology and an optimized assay protocol, we have identified and validated a variety of functional combinations of the enzyme components. With the established protocol, each enzyme in the catalytic cascade has been assessed for their activity towards the generation of free AMP. In addition, inhibition profiles of the ubiquitinating enzymes have been obtained using the assay system, further demonstrating their potential to be used in high-throughput screening to identify lead compounds for drug discovery and development programs.

Name	Catalog Number	Species	Tag	Expression System	Sequence	Genbank Number
SAE1/UBA2 Complex, Active	U208-380G	Human	His/GST	Sf9 insect cells using baculovirus	Full Length	NM_005500
UBA1 (UBE1), Active	U201-380G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_003334
UBA6, Active	U206-380G	Human	GST	Sf9 insect cells using baculovirus	Full Length	AL832458
UBA7(UBE1L), Active	U207-380G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_003335
UBA2 (SAE2) Protein	U202-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_005499
UBA3 (UBE1C) Protein	U203-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC022853
UBLE1A (SAE1) Protein	U208-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_005500

ARGININE DEIMINASES

Post-translational modification of cellular proteins plays a crucial role in cell survival, cell signaling and overall maintenance of cellular health. Citrullination is one of the many crucial protein modifications catalyzed by a family of enzymes called Protein Arginine Deiminases (PADs). PADs catalyze the hydrolysis of peptidyl-arginine to form peptidyl-citrulline on important cellular proteins such as histones, thereby regulating multiple cell signaling pathways, and biochemical processes.

The PAD family consists of five members of calcium-dependent isozymes PAD1, 2, 3, 4, and 6. Citrullination or hypercitrullination of proteins induces a structural change, modifying their biological function and their interaction mechanisms with other cellular proteins. Because of this property, numerous notable studies have established PADs as an important class of targets for drug discovery projects related to many human diseases such as cancer, autoimmune diseases (rheumatoid arthritis), and neurodegeneration.

SignalChem’s scientists have produced all five high-quality recombinant mammalian PADs and have developed sensitive and reliable PAD assay cocktails to facilitate your drug discovery and development research. These are effective in vitro tools to investigate protein citrullination in various human diseases.

Name	Catalog Number	Species	Tag	Expression System	Sequence	Genbank Number
PAD Cocktail, Active	P312-37C		GST	Sf9 insect cells using baculovirus	Full Length	NM_013358 (PAD1)
PAD1, Active	P312-310G	Human	GST	Sf9 insect cells using baculovirus	Full length	NM_013358
PAD2, Active	P312-310BG	Mouse	GST	Sf9 insect cells using baculovirus	Full length	BC049947
PAD2, Active	P312-318BG	Human	GST	Sf9 insect cells using baculovirus	Full length	NM_007365
PAD3, Active	P312-310CG	Human	GST	Sf9 insect cells using baculovirus	Full length	NM_016233
PAD4, Active	P312-310DG	Human	GST	Sf9 insect cells using baculovirus	Full length	BC025718
PAD6 Protein	P312-30FG	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_207421

GTPASES & ATPASES

ATPases and GTPases are two different families of hydrolase enzymes that catalyze the hydrolysis of the third phosphate of ATP and GTP into ADP and GDP respectively. While human ATPases mainly facilitate the transport of cellular metabolites by harnessing the energy released from the ATP hydrolysis, the GTPases conventionally act as “molecular switches” that are essential mediators of signal transduction in the cells. Dephosphorylation of ATP and GTP is an important chemical process in all forms of life, therefore ATPases and GTPases are spread ubiquitously across the plant and the animal kingdom. Mutations in members of these families of enzymes have been observed in many human diseases such as cancer and metabolic disorders. For example, the founding member of the small GTPase Superfamily, Ras, is mutated in about 15% of all human tumors.

SignalChem manufactures high-quality active GTPase and ATPase enzymes such as G protein α subunits, Ras, Rac, and Rho. Our recombinant ATPases/GTPases are perfect candidates to be included in enzyme activity assays and their inhibitor profiling studies. SignalChem’s active GTPases are tested exclusively using Promega’s GTPase-Glo™ Assay.

Name	Catalog Number	Species	Tag	Expression System	Sequence	Genbank Number
CDC42, Active	C108-310GH	Human	GST/HIS	E.coli	Full Length	NM_001791
GNAI1, Active	G121-310G	Human	GST	E.coli	2-end	NM_002069
GNAI2, Active	G122-310G	Human	GST	E.coli	2-end	NM_002070
GNAI3, Active	G123-310G	Human	GST	E.coli	2-end	NM_006496
GNAS, Active	G127-310G	Human	GST	E.coli	Full Length	NM_000516
HRAS1 (Mature form), Active	R105-310H	Human	HIS	E.coli	2-186	NM_005343
KRAS (Mature form), Active	R106-310H	Human	HIS	E.coli	2-186	NM_033360
NRAS (Mature form), Active	R107-310H	Human	HIS	E.coli	1-186	NM_002524
RAC1, Active	R103-310G	Human	GST	E.coli	Full Length	NM_006908
RAC2, Active	R104-310H	Human	HIS	E.coli	Full Length	NM_002872
RAC3, Active	R102-310H	Human	HIS	E.coli	Full Length	NM_005052
RhoA (Mature form), Active	R144-310H	Human	HIS	E.coli	1-190	NM_001664
RhoC (Mature form), Active	R144-310CH	Human	HIS	E.coli	1-190	NM_175744
RhoD (Mature form), Active	R144-310DH	Human	HIS	E.coli	1-207	BC001338
VCP, Active	V116-310G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_007126

PHOSPHODIESTERASES

Phosphodiesterases (PDEs) play a central role in the regulation of various signaling cascades by specifically hydrolyzing the cyclic nucleotides, cAMP, and cGMP into their nucleoside 5’ -– monophosphate form AMP and GMP respectively. Hydrolysis of these nucleotides results in the breakdown of these secondary messengers and the abrogation of downstream signaling. Given the importance of these enzymes in the regulation of cAMP/cGMP signaling, PDEs have been identified as an important class of potential drug targets in diseases such as cancer, neurodegeneration, coronary heart diseases, asthma and more.

Signalchem has successfully manufactured 19 of the 24 phosphodiesterases from the human genome as active enzymes. Our recombinant PDEs are compatible with their substrates cAMP and cGMP and are ready to be included in enzyme activity and inhibitor profiling assays. Our PDEs are tested exclusively using Promega’s PDE-Glo™ Assay technology.

Name	Catalog Number	Species	Tag	Expression System	Sequence	Genbank Number
PDE10A, Active	P98-31G	Human	GST	Sf9 insect cells using baculovirus	71-end	NM_006661
PDE1A, Active	P89-30H	Human	HIS	Sf9 insect cells using baculovirus	Full Length	NM_005019
PDE1B, Active	P89-30BG	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_000924
PDE1C, Active	P89-30CG	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_005020
PDE2A, Active	P90-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_002599
PDE3A, Active	P91-31G	Human	GST	Sf9 insect cells using baculovirus	669-end	NM_000921
PDE3B, Active	P91-31BG	Human	GST	Sf9 insect cells using baculovirus	654-end	NM_000922
PDE4A, Active	P92-31G	Human	GST	Sf9 insect cells using baculovirus	332-end	NM_001111307
PDE4B, Active	P92-31BG	Human	GST	Sf9 insect cells using baculovirus	305-end	NM_002600
PDE4C, Active	P92-31CG	Human	GST	Sf9 insect cells using baculovirus	287-end	NM_000923
PDE4D, Active	P92-31DG	Human	GST	Sf9 insect cells using baculovirus	225-end	NM_006203
PDE5A, Active	P93-31G	Human	GST	Sf9 insect cells using baculovirus	537-end	NM_001083
PDE6A, Active	P94-31G	Human	GST	Sf9 insect cells using baculovirus	31-end	NM_000440
PDE6B, Active	P94-30BG	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_000283
PDE7A, Active	P95-31G	Human	GST	Sf9 insect cells using baculovirus	104-end	NM_002603
PDE7B, Active	P95-30BG	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_018945
PDE8A, Active	P96-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_002605
PDE8B, Active	P96-31BG	Human	GST	Sf9 insect cells using baculovirus	113-end	NM_003719
PDE9A, Active	P97-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_001001567

OTHER ENZYMES

High-quality recombinant proteins are crucial for life science research and drug discovery projects. SignalChem offers a broad range of recombinant proteins including Dihydropyrimidinase, Dioxygenases, lysyl oxidases, superoxide dismutases, and phospholipase C of superior purity. Our in-house team with over 100 years of combined experience in recombinant protein biochemistry stands behind the production and quality of one of the most comprehensive recombinant protein product line in the world.

Nucleases

Other Enzymes

Proteases

Name	Catalog Number	Species	Tag	Expression System	Sequence	Genbank Number
DPYSL2 Protein	D470-30BG	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC056408

Assay Kits

Protein Arginine Deiminases Activity Detection Kits

Detection Kits

Catalogue Number	Product Name
P312-863	PAD Detection Kit

Inhibitor Screening Kits

Catalogue Number	Product Name
P37C-863	PAD Cocktail Inhibitor Screening Kit
P318BG-863	PAD2 (human) Inhibitor Screening Kit
P310G-863	PAD1 Inhibitor Screening Kit
P310DG-863	PAD4 Inhibitor Screening Kit
P310CG-863	PAD3 Inhibitor Screening Kit
P310BG-863	PAD2 (mouse) Inhibitor Screening Kit

Antibodies

SignalChem understands how important it is for the researchers to source high-quality antibodies while working on research projects involving new proteins and other antigens of interest. SignalChem offers a comprehensive range of polyclonal and monoclonal antibodies including primary & secondary antibodies, paralog-specific antibodies, modification antibodies, phospho-specific antibodies, and tag antibodies to support life science research. We also work with scientists to develop custom recombinant antibodies in various cell systems, ready to be used in different immunoassays.

Tag Antibodies

Name	Catalog Number	Host	Cited Application	Immunogen
Anti-DYKDDDDK	F51-61M	Mouse, IgG2b, FG4R	WB, ELISA, IP, IS	DYKDDDDK peptide conjugated to KLH
Anti-DYKDDDDK	F51-63R	Rabbit	WB	DYKDDDDK peptide conjugated to KLH
Anti-GFP	G46-60M	Mouse, IgG1, GF28R	WB	GFP N-terminal peptide with KLH conjugate from Aequorea Victoria Jellyfish
Anti-GST	G52-61M	Mouse, IgG2a, G2R	WB	Purified GST
Anti-HA	H98-61M	Mouse, IgG3, C10	WB, ELISA, IP, IS
Anti-HA	H98-63R	Rabbit Polyclonal	WB, ELISA, IP, IS
Anti-His	H99-61M	Mouse, IgG2b, EH158	WB, ELISA, IP, IS	HHHHHH peptide conjugated to KLH
Anti-His	H99-63R	Rabbit	WB, ELISA, IP, IS
Anti-Myc	M86-61M	Mouse, IgG1, M19	WB, ELISA, IP, IS	EQKLISEED peptide conjugated to KLH
Anti-RFP	R46-61M	Mouse, IgG1, RF5R	WB	RFP from the Discosoma sea anemone N-terminal peptide-KLH conjugates
Anti-V5	V05-61M	Mouse, IgG1, V4RR	WB, ELISA, IP, IS	GKPIPNPLLGLDST peptide conjugated to KLH

Primary Antibodies

View All Primary Antibodies

Monoclonal Primary Antibody - Unconjugated

Polyclonal Primary Antibody

Name	Catalog Number	Host	Cited Application	Immunogen
Anti-4EBP1	E35-563R	Rabbit, IgG	WB, ELISA, ICC	4EBP1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human 4EBP1
Anti-ADD1	A38-63R	Rabbit	WB	Synthetic peptide corresponding to amino acids 724-728 of human ADD1 (P35611)
Anti-AFX	A42-63R	Rabbit	WB	Synthetic peptide corresponding to amino acids 195-199 of human AFX
Anti-AKT (pan)	A16-63R	Rabbit	WB	Synthetic peptide corresponding to amino acids 471-475 of human AKT
Anti-AKT (pan)	A16-63BR	Rabbit	WB	Synthetic peptide corresponding to amino acids 313-317 of human AKT1 (protein accession number P31749)
Anti-AKT1	A16-60M	Mouse, Monoclonal, 9A4	WB	Recombinant full length active AKT1 protein
Anti-AKT1	A16-63CR	Rabbit, IgG	WB, ELISA, IF, IHC	AKT1 antibody was raised against a 16 amino acid synthetic peptide from near the amino-terminus of human AKT1
Anti-AKT2	A17-61A	Rat, IgG2a	ELISA, IHC, WB	The antibody was produced against synthesized peptide corresponding to internal residues of the human AKT2 protein.
Anti-AKT2/3	A17-63BR	Rabbit	WB	Protein Code: P31751
Anti-AKT3	A18-61M	Mouse, IgG1	ELISA, IHC, WB	The antibody was produced against synthesized peptide corresponding to internal residues of the human AKT3 protein.
Anti-ALK2	A06-63R	Rabbit, IgG	WB, ELISA	ALK2 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of the human ALK2
Anti-ALK4	A07-63R	Rabbit, IgG	WB, ELISA	ALK4 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of the human ALK4
Anti-ALK7	A08-63R	Rabbit, IgG	WB, ELISA	ALK7 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of the human ALK7
Anti-alpha 1-Actin	A03-360M	Mouse, IgG1, C4	WB, IF, IHC	Chicken gizzard actin
Anti-AMPK alpha 1/2	P47-63BR	Rabbit	WB	Synthetic peptide corresponding to amino acids 170-174 of human AMPK
Anti-Amyloid beta	A06-563R	Rabbit, IgG	WB, ELISA, IF, IHC	Amyloid beta antibody was raised against a synthetic peptide corresponding to the amino terminus (aa 1-14) of human beta amyloid conjugated to KLH using maleimide.
Anti-AOS1	A80-63R	Rabbit	WB	Synthetic peptide of c-terminal amino acid sequence of human AOS1 (Q9UBE0)
Anti-APBA1	A86-363R	Rabbit, IgG	WB	Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH
Anti-APC1	A87-363R	Rabbit, IgG	WB, ELISA, IHC	APC1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human APC1
Anti-APC1 (Ser355)	A87-363BR	Rabbit, IgG	ELISA, IP, WB	The antibody was produced against synthesized peptide corresponding to amino acids 351-359 of Human APC1.

Secondary Antibodies

Name	Catalog Number	Host	Cited Application	Immunogen
Anti-GOAT, HRP Conjugated	G34-62DC	Chicken, IgG	Ideal working dilutions for each application should be empirically determined by the investigator.	The antibody was produced against goat IgG whole molecule
Anti-Mouse HRP	G32-62G	Goat Polyclonal	WB, ELISA	Affinity purified mouse immunoglobulins
Anti-MOUSE, AP Conjugated	M32-62ER	Rabbit, IgG	IHC, ELISA, WB (Ideal working dilutions for each application should be empirically determined by the investigator.)	The antibody was produced against mouse IgG whole molecule
Anti-MOUSE, Biotin Conjugated	M32-62BG	Goat, IgG	WB, ELISA, IHC (Ideal working dilutions for each application should be empirically determined by the investigator.)	The antibody was produced against mouse IgG whole molecule in goat.
Anti-MOUSE, Fluorescein Conjugated	M32-62CG	Goat, IgG	ELISA, IF Microscopy, Flow Cytometry (Ideal working dilutions for each application should be empirically determined by the investigator.)	The antibody was produced against mouse IgG whole molecule.
Anti-MOUSE, Rhodamine Conjugate	M32-62FG	Goat, IgG	ELISA, Flow Cytometry, IF Microscopy (Ideal working dilutions for each application should be empirically determined by the investigator.)	The antibody was produced against mouse IgG whole molecule.
Anti-Rabbit HRP	G33-62G	Goat Polyclonal	WB, ELISA	Affinity purified rabbit immunoglobulins
Anti-RABBIT, AP Conjugated	R33-62EG	Goat, IgG	ELISA, IHC, WB (Ideal working dilutions for each application should be empirically determined by the investigator.)	The antibody was produced against Rabbit IgG whole molecule in goat.
Anti-RABBIT, Biotin Conjugated	R33-62BG	Rabbit, IgG	ELISA, IHC, WB (Ideal working dilutions for each application should be empirically determined by the investigator.)	The antibody was produced against Rabbit IgG whole molecule in goats
Anti-RABBIT, Fluorescein Conjugated	R33-62CG	Goat, IgG	ELISA, Flow Cytometry, IF Microscopy (Ideal working dilutions for each application should be empirically determined by the investigator.)	The antibody was produced against Rabbit IgG whole molecule
Anti-RABBIT, Rhodamine Conjugated	R33-62FG	Goat, IgG	FLISA, FC, IF (Ideal working dilutions for each application should be empirically determined by the investigator.)	The antibody was produced against Rabbit IgG whole molecule.
Anti-RAT, HRP Conjugated	A37-62DG	Goat, IgG	ELISA, IHC, WB (Ideal working dilutions for each application should be empirically determined by the investigator.)	The antibody was produced against rat IgG whole molecule.
Anti-SHEEP, HRP Conjugated	S36-62DD	Donkey, IgG	ELISA, IHC. WB (Ideal working dilutions for each application should be empirically determined by the investigator.)	The antibody was produced against synthesized Sheep IgG whole molecule in donkey.

Isoform Specific Antibodies

Name	Catalogue Number	Host	Cited Application	Immunogen
Anti-AKT3	A18-63R	Rabbit	WB	Protein Code: Q9Y243
Anti-AMPK alpha 1	P47-63R	Rabbit	WB	Synthetic peptide corresponding to amino acids 483-487 of human AMPKα
Anti-CAMK1	C07-60M	Mouse, IgG1, 5H8	WB	Human recombinant full length CAMK1 protein
Anti-CHK2	C48-60M	Mouse, IgG1, 7F9	WB	Human full length recombinant CHK1
Anti-ERK1	M29-60M	Mouse, IgG1, E32	WB, IP	Recombinant full length ERK1 protein
Anti-ERK1	M29-60BM	Mouse, IgG1, E19	WB, IP	Recombinant full length ERK1 protein
Anti-GSK3 alpha	G08-63R	Rabbit	WB	Protein Code P49840
Anti-GSK3 beta	G09-60M	Mouse, IgG1, 6C2	WB	Recombinant full length active GSK3β protein
Anti-HSP90 beta	H36-63R	Rabbit	WB	Synthetic peptide corresponding to amino acids 252-256 of human HSP90β
Anti-JAK2	J02-63R	Rabbit	WB	Synthetic peptide corresponding to amino acids 1005-1009 of human JAK2
Anti-JNK1	M33-63R	Rabbit	WB	Protein Code: P45983
Anti-JNK2	M34-60M	Mouse, IgG2, 2G12	WB, IP	Human recombinant full length JNK2 protein
Anti-MEK1	M02-60M	Mouse, IgG1, 7F1	WB, IP	Recombinant full length humanMEK1 protein
Anti-MEK2	M03-63R	Rabbit	WB	Synthetic peptide corresponding to amino acids 392-396 of human MEK2
Anti-p38 alpha	M39-63R	Rabbit	WB	C-terminal peptide of p38α conjugated to ovalbumin
Anti-p38 beta	M36-63R	Rabbit	WB	C-terminal peptide of p38β conjugated to ovalbumin
Anti-p38 gamma	M37-63R	Rabbit	WB	C-terminal peptide of p38γ conjugated to ovalbumin
Anti-PAK1	P02-63R	Rabbit	WB	Synthetic peptide corresponding to amino acids 210-214 of human PAK1
Anti-PKC delta	P64-63R	Rabbit	WB	Synthetic peptide corresponding to amino acids 643-647 of human PKCδ
Anti-PKC delta	P64-63CR	Rabbit	WB	Synthesized peptide derived from C-terminus of human PKCδ protein
Anti-PKC mu	P72-63R	Rabbit	WB	Synthetic peptide corresponding to amino acids 736-740 of human PKCμ
Anti-PKC theta	P74-63R	Rabbit	WB	Synthetic peptide corresponding to amino acids 674-678 of human PKCθ
Anti-SMAD2	S11-63R	Rabbit	WB	Synthetic peptide corresponding to amino acids 218-222 of human SMAD2
Anti-STAT1	S52-63R	Rabbit	WB	Synthetic peptide corresponding to amino acids 699-703 of human STAT1
Anti-STAT3	S54-63BR	Rabbit	WB, IP	Synthetic peptide corresponding to amino acids 725-729 of human STAT3
Anti-STAT3	S54-63R	Rabbit	WB	Protein Code: P40763
Anti-STAT4	S55-63R	Rabbit	WB	Synthetic peptide corresponding to amino acids 691-695 of human STAT4
Anti-STAT5	S56-63R	Rabbit	WB	Protein Code: P42229
Anti-STAT5	S56-63BR	Rabbit	WB	Synthetic peptide corresponding to amino acids 692-696 of human STAT5 (protein accession number P42229)

Modification Antibodies

Name	Catalogue Number	Host	Cited Application	Immunogen
Anti-5-Methylated Cytosine (5-methyl cytosine)	C92-67S	Sheep, IgG	WB, IP	5-Methylated Cytosine (5-methyl cytosine)
Anti-Acetylated Lysine	L95-66R	Rabbit	WB, ELISA, PI, IHC, IF	Acetylated lysine-KLH conjugates
Anti-Acetylated Lysine, Biotin Conjugated	L95-66BR	Rabbit	WB, ELISA, IF, IHC, IP	Acetylated lysine-KLH conjugates
Anti-Acetylated Lysine, FITC Conjugated	L95-66CR	Rabbit	For direct immunofluorescence assay	Acetylated lysine-KLH conjugates
Anti-Acetylated Lysine, HRP Conjugated	L95-66DR	Rabbit	WB, ELISA, IF, IHC, IP	Acetylated Lysine-KLH conjugates
Anti-Methylated Arginine (NG, NG-Di-methyl)	A96-67R	Rabbit	WB, ELISA, IF, IHC, IP	NG, NG-di-methylated arginine-KLH conjugates
Anti-Methylated Arginine (NG-Mono-methyl)	A95-67R	Rabbit	WB, ELISA, IF, IHC, IP	NG-mono-methylated arginine-KLH conjugates
Anti-Methylated Lysine (Tri- epsilon-N-methyl)	L95-67R	Rabbit	WB, ELISA, IF, IHC, IP	Methylated lysine-KLH conjugates.
Anti-Methylated Lysine (Tri- epsilon-N-methyl), Biotin Conjugated	L95-67BR	Rabbit	WB, ELISA, IF, IHC	Methylated lysine-KLH conjugates.
Anti-Methylated Lysine (Tri- epsilon-N-methyl), FITC Conjugated	L95-67CR	Rabbit	For direct immunofluorescence assay	Methylated lysine-KLH conjugates.
Anti-Methylated Lysine (Tri- epsilon-N-methyl), HRP Conjugated	L95-67DR	Rabbit	WB, ELISA, IF, IHC, IP	Methylated lysine-KLH conjugates.
Anti-Phosphoserine (Anti-pS)	S95-65R	Rabbit	WB, ELISA, IHC, IP	Phosphoserine peptide-KLH conjugates
Anti-Phosphoserine (Anti-pS), Biotin Conjugated	S95-65BR	Rabbit	WB, ELISA	Phosphoserine peptide-KLH conjugates
Anti-Phosphothreonine (Anti-pT)	T95-65R	Rabbit	WB, ELISA, IHC, IP	Phosphothreonine peptide-KLH conjugates
Anti-Phosphothreonine (Anti-pT), Biotin Conjugated	T95-65BR	Rabbit	WB, ELISA	Phosphothreonine peptide-KLH conjugates
Anti-Phosphotyrosine (Anti-pY)	T96-65R	Rabbit	WB, ELISA, IHC, IP	Phosphotyrosine peptide-KLH conjugates
Anti-Phosphotyrosine (Anti-pY), Biotin Conjugated	T96-65BR	Rabbit	WB, ELISA, IHC, IP	Phosphotyrosine peptide-KLH conjugates

Phospho Specific Antibodies

View all Phospho Specific Antibodies

Name	Catalogue Number	Host	Cited Application	Immunogen
Anti-5-Methylated Cytosine (5-methyl cytosine)	C92-67S	Sheep, IgG	WB, IP	5-Methylated Cytosine (5-methyl cytosine)
Anti-Acetylated Lysine	L95-66R	Rabbit	WB, ELISA, PI, IHC, IF	Acetylated lysine-KLH conjugates
Anti-Acetylated Lysine, Biotin Conjugated	L95-66BR	Rabbit	WB, ELISA, IF, IHC, IP	Acetylated lysine-KLH conjugates
Anti-Acetylated Lysine, FITC Conjugated	L95-66CR	Rabbit	For direct immunofluorescence assay	Acetylated lysine-KLH conjugates
Anti-Acetylated Lysine, HRP Conjugated	L95-66DR	Rabbit	WB, ELISA, IF, IHC, IP	Acetylated Lysine-KLH conjugates
Anti-Methylated Arginine (NG, NG-Di-methyl)	A96-67R	Rabbit	WB, ELISA, IF, IHC, IP	NG, NG-di-methylated arginine-KLH conjugates
Anti-Methylated Arginine (NG-Mono-methyl)	A95-67R	Rabbit	WB, ELISA, IF, IHC, IP	NG-mono-methylated arginine-KLH conjugates
Anti-Methylated Lysine (Tri- epsilon-N-methyl)	L95-67R	Rabbit	WB, ELISA, IF, IHC, IP	Methylated lysine-KLH conjugates.
Anti-Methylated Lysine (Tri- epsilon-N-methyl), Biotin Conjugated	L95-67BR	Rabbit	WB, ELISA, IF, IHC	Methylated lysine-KLH conjugates.
Anti-Methylated Lysine (Tri- epsilon-N-methyl), FITC Conjugated	L95-67CR	Rabbit	For direct immunofluorescence assay	Methylated lysine-KLH conjugates.
Anti-Methylated Lysine (Tri- epsilon-N-methyl), HRP Conjugated	L95-67DR	Rabbit	WB, ELISA, IF, IHC, IP	Methylated lysine-KLH conjugates.
Anti-Phosphoserine (Anti-pS)	S95-65R	Rabbit	WB, ELISA, IHC, IP	Phosphoserine peptide-KLH conjugates
Anti-Phosphoserine (Anti-pS), Biotin Conjugated	S95-65BR	Rabbit	WB, ELISA	Phosphoserine peptide-KLH conjugates
Anti-Phosphothreonine (Anti-pT)	T95-65R	Rabbit	WB, ELISA, IHC, IP	Phosphothreonine peptide-KLH conjugates
Anti-Phosphothreonine (Anti-pT), Biotin Conjugated	T95-65BR	Rabbit	WB, ELISA	Phosphothreonine peptide-KLH conjugates
Anti-Phosphotyrosine (Anti-pY)	T96-65R	Rabbit	WB, ELISA, IHC, IP	Phosphotyrosine peptide-KLH conjugates
Anti-Phosphotyrosine (Anti-pY), Biotin Conjugated	T96-65BR	Rabbit	WB, ELISA, IHC, IP	Phosphotyrosine peptide-KLH conjugates

Proteins

SIGNALING PROTEINS

SignalChem manufactures a broad range of recombinant cell signaling proteins paying thorough attention to quality to assist in global academic research and drug discovery efforts. Our comprehensive family offering of signaling protein products have been utilized by many pharmaceutical companies in their research and drug discovery programs. Explore Signalchem’s cell signaling protein product range to study molecular and cell signaling pathways.

Adaptor Proteins

Adaptor Proteins

Name	Catalog Number	Species	Tag	Expression System	Sequence	Genbank Number
FADD Protein	F02-30H	Human	HIS	E.coli	Full Length	NM_003824
GAB1 Protein	G03-30H	Human	HIS	Sf9 insect cells using baculovirus	Full Length	NM_207123
GRB2 Protein	G45-30G	Human	GST	E.coli	Full Length	NM_002086
GRB7 Protein	G45-30GG	Human	GST	E.coli	Full Length	NM_001030002
NCK Protein	N06-30H	Human	HIS	Sf9 insect cells using baculovirus	Full Length	NM_006153
TRAF1 Protein	T56-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_005658
TRAF2 Protein	T57-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_021138

Apoptosis Proteins

Name	Catalog Number	Species	Tag	Expression System	Sequence	Genbank Number
ATG13 Protein	A113-30G	Human	GST	E.coli	Full Length	NM_001142673
BAD Protein	B03-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_032989
BAG1 Protein	B05-31G	Human	GST	E.coli	72-end	NM_004323
BAX Protein	B14-30G	Human	GST	E.coli	Full Length	NM_004324
BCL2 Protein	B25-30G	Human	GST	E.coli	Full Length	NM_000633
BID Protein	B59-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC036364
XIAP Protein	X08-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_001167

Cell Cycle Proteins

Cell Cycle Proteins

Name	Catalog Number	Species	Tag	Expression System	Sequence	Genbank Number
CDC7 Protein	C26-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_003503
CDK2 Protein	C29-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_001798
CDK5P25 Protein	C34-31G	Human	GST	Sf9 insect cells using baculovirus	98-end	NM_003885
CDK5P35 Protein	C34-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_003885
CDKN3 Protein	C01-34G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_005192
Cyclin E2 Protein	C82-30BG	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_057749
Cyclin T2 Protein	C86-30BG	Human	GST	Sf9 insect cells using baculovirus	Full-Length	NM_001241
Cyclin Y Protein	C87-30G	Human	GST	Sf9 insect cells using baculovirus	2-end	NM_145012
CyclinA2 Protein	C78-30BG	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_001237
CyclinC Protein	C80-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_005190
CyclinD1 Protein	C81-30G	Human	GST	E.coli	Full Length	NM_053056
CyclinD1 Protein	C81-30BG	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_053056
CyclinD3 Protein	C81-30B3G	Human	GST	Sf9 insect cells using baculovirus	Full length	NM_001760
CyclinE1 Protein	C82-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_001238
CyclinH Protein	C83-30H	Human	HIS	Sf9 insect cells using baculovirus	Full Length	NM_001239
CyclinK Protein	C84-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC015935
CyclinO Protein	C85-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_021147
MDM2 (1-118) Protein	M45-31BH	Human	HIS	E.coli	1-118	NM_002392
MDM2 Protein	M45-31G	Human	GST	E.coli	202-end	NM_002392
MDM4 (1-137) Protein	M47-31BH	Human	HIS	E.coli	1-137	NM_002393
p15INK4B Protein	C31-30G	Human	GST	E.coli	Full Length	NM_004936
p16INK4A Protein	C30-30G	Human	GST	E.coli	Full Length	NM_000077
p18INK4C Protein	C32-30G	Human	GST	E.coli	Full Length	NM_001262
p19INK4D Protein	C33-30G	Human	GST	E.coli	Full Length	NM_001800
p21CIP1 Protein	C27-30G	Human	GST	E.coli	Full Length	NM_000389
p27KIP1 Protein	C28-30G	Human	GST	E.coli	Full Length	NM_004064
p33ING1 Protein	I32-30H	Human	HIS	Sf9 insect cells using baculovirus	Full Length	NM_198219
p33ING2 Protein	I32-30BG	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_001564
p53 Protein	P05-30G	Human	GST	E.coli	Full Length	NM_000546
p53 Protein	P05-30BG	Human	GST	Hi5 insect cells using baculovirus	Full Length	NM_000546
p63 Protein	P06-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_001114980
p73 alpha Protein	P08-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_005427
p73 beta Protein	P08-30BG	Human	GST	Sf9 insect cells using baculovirus	Full Length	O15350-2
p73 gamma Protein	P08-30CG	Human	GST	Sf9 insect cells using baculovirus	Full Length	O15350-3

Cell Stress & Chaperone Proteins

Cell Stress & Chaperone Proteins

Name	Catalog Number	Species	Tag	Expression System	Sequence	Genbank Number
14-3-3 alpha/beta Protein	Y71-30G	Human	GST	E.coli	Full Length	NM_003404
14-3-3 alpha/beta Protein	Y71-30N	Human	TAG-Free	E.coli	Full Length	NM_003404
14-3-3 alpha/beta Protein	Y71-30H	Human	HIS	Sf9 insect cells using baculovirus	Full Length	NM_003404
14-3-3 alpha/beta Protein	Y71-30BH	Mouse	HIS	Sf9 insect cells using baculovirus	Full Length	NM_018753
14-3-3 epsilon Protein	Y75-30G	Human	GST	E.coli	Full Length	NM_006761
14-3-3 epsilon Protein	Y75-30N	Human	TAG-Free	E.coli	Full Length	NM_006761
14-3-3 epsilon Protein	Y75-30H	Human	HIS	Sf9 insect cells using baculovirus	YWHAE, MDS, MDCR, KCIP-1, 14-3-3E, FLJ45465	NM_006761
14-3-3 epsilon Protein	Y75-30BH	Mouse	HIS	Sf9 insect cells using baculovirus		NM_009536
14-3-3 sigma Protein	Y86-30G	Human	GST	E.coli	Full Length	NM_006142
14-3-3 sigma Protein	Y86-30N	Human	TAG-Free	E.coli	Full Length	NM_006142
14-3-3 sigma Protein	Y86-30H	Human	HIS	Sf9 insect cells using baculovirus	Full Length	NM_006142
14-3-3 theta Protein	Y84-30G	Human	GST	E.coli	Full Length	NM_006826
14-3-3 theta Protein	Y84-30N	Human	TAG-Free	E.coli	Full Length	NM_006826
14-3-3 theta Protein	Y84-30H	Human	HIS	Sf9 insect cells using baculovirus	Full Length	NM_006826
14-3-3 theta Protein	Y84-30BH	Mouse	HIS	Sf9 insect cells using baculovirus	Full Length	NM_011739
14-3-3 zeta Protein	Y92-30G	Human	GST	E.coli	Full Length	NM_003406
14-3-3 zeta Protein	Y92-30N	Human	TAG-Free	E.coli	Full Length	NM_003406
14-3-3 zeta Protein	Y92-30H	Human	HIS	Sf9 insect cells using baculovirus	Full Length	NM_003406
14-3-3 zeta Protein	Y92-30BH	Mouse	HIS	Sf9 insect cells using baculovirus	Full Length	NM_011740
CRYAA Protein	C256-30H	Human	HIS	E.coli	Full Length	NM_000394
GST Protein	G52-30U	Artificial	TAG-Free	E.coli	1-226	U13850
HO1 Protein	H20-30G	Human	GST	E.coli	Full Length	NM_002133
HO2 Protein	H21-30G	Human	GST	E.coli	Full Length	NM_002134
HSP10 Protein	H29-54G	Human	GST	E.coli	Full Length	NM_002157
HSP27 Protein	H30-54G	Human	GST	E.coli	Full Length	NM_001540
HSP40 Protein	H32-54G	Human	GST	E.coli	Full Length	NM_006145
HSP60 Protein	H33-54G	Human	GST	E.coli	Full Length	NM_002156
HSP70 Protein	H34-54G	Human	GST	E.coli	Full Length	NM_005345
HSP70 Protein	H34-54H	Human	HIS	Sf9 insect cells using baculovirus	Full Length	NM_005345
HSP90 alpha Protein	H36-50H	Human	HIS	Sf9 insect cells using baculovirus	Full Length	NM_005348
HSP90 beta Protein	H36-54H	Human	HIS	Sf9 insect cells using baculovirus	Full Length	NM_ 007355
Mortalin Protein	M54-31G	Human	GST	Sf9 insect cells using baculovirus	47-end	NM_004134
PARK7 (DJ-1) Protein	P219-31H	Human	HIS	E.coli	19-end	NM_007262

Color Proteins

Name	Catalog Number	Species	Tag	Expression System	Sequence	Genbank Number
BFP Protein	B620-30H		HIS	E.coli	228-end	DQ399412
eRFP Protein	R620-30BH		HIS	E.coli	Full Length	AY130757
Red Protein	R620-30CH		HIS	E.coli	Full Length	EU498726
RFP Protein	R620-30H		HIS	E.coli	Full Length	AAF03369
Y1BFP Protein	B620-30BH		HIS	E.coli	Full Length	BAG71497

Microtubule & Actin Associated Proteins

Name	Catalog Number	Species	Tag	Expression System	Sequence	Genbank Number
Alpha-synuclein Protein	S22-30H	Human	HIS	E.coli	Full Length	NM_000345
Beta-synuclein Protein	S22-30BH	Human		E.coli	Full Length	NM_003085
Cofilin 1 Protein	C37-54H	Human	HIS	E.coli	Full Length	NM_005507
Gamma-synuclein Protein	S22-30CH	Human	HIS	E.coli	Full Length	NM_003087
INCENP Protein	I30-31H	Human	HIS	Sf9 insect cells using baculovirus	783-end	NM_001040694
Stathmin Protein	S71-30H	Human	HIS	E.coli	Full Length	BC014353
TPM3 Protein	T36-30H	Human	HIS	E.coli	Full Length	NM_152263
TPX2 Protein	T40-30H	Human	HIS	Sf9 insect cells using baculovirus	Full Length	NM_012112
Troponin1 Protein	T68-30H	Human	HIS	E.coli	Full Length	NM_003281
Tubulin Beta (TUBB1) Protein	T92-50G	Human	GST	E.coli	Full Length	NM_178014
Vimentin Protein	V49-30H	Human	HIS	E.coli	Full Length	NM_003380

Regulatory Proteins

View all

Transcription Proteins

Name	Catalog Number	Species	Tag	Expression System	Sequence	Genbank Number
ATF1 Protein	A09-54G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_005171
c-JUN Protein	J05-55G	Human	GST	E.coli	Jan-79	NM_002228
Catenin alpha Protein	C05-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_001903
Catenin beta Protein	C06-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_001904
CBP Protein	C07-31G	Human	GST	E.coli	1319-1710	NM_004380
CREB1 Protein	C49-55G	Rat	GST	E.coli	1-280	NM_031017
ELK1 Protein	E64-30G	Human	GST	E.coli	Full Length	NM_005229
FOS Protein	F66-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_005252
FOXL2 Protein	F71-30BG	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_023067
HIF1B Protein	H07-30BG	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_001668
HSF1 Protein	H25-30G	Human	GST	E.coli	Full Length	NM_005526
IkBA Protein	I20-30G	Human	GST	E.coli	Full Length	NM_020529
IkBA Protein	I20-31G	Human	GST	E.coli	1-175	NM_020529
IkBB Protein	I21-30G	Human	GST	E.coli	Full Length	NM_002503
NFATC1 Protein	N12-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_172390
NFKB2 Protein	N13-31G	Human	GST	Sf9 insect cells using baculovirus	1-454	NM_002502
Rel B Protein	R30-30H	Human	HIS	Sf9 insect cells using baculovirus	Full Length	NM_006509
SMAD1 Protein	S10-30G	Human	GST	E.coli	Full Length	NM_005900
SMAD2 (del SXS) Protein	S11-31G	Human	GST	E.coli	C-terminal SXS deletion	NM_001003652
SMAD2 Protein	S11-30G	Human	GST	E.coli	Full Length	NM_001003652
SMAD3 (del SXS) Protein	S12-31G	Human	GST	E.coli	C-terminal SXS deletion	NM_005902
SMAD3 Protein	S12-30G	Human	GST	E.coli	Full Length	NM_005902
SMAD4 Protein	S13-30G	Human	GST	E.coli	Full Length	NM_005359
SMAD5 Protein	S14-30G	Human	GST	E.coli	Full Length	NM_005903
SMAD9 Protein	S17-30G	Human	GST	E.coli	Full Length	NM_001127217
STAT1 alpha Protein	S52-50G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_139266
STAT1 beta Protein	S52-54G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_139266
STAT2 Protein	S53-54G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_005419
STAT3 Protein	S54-54G	Human	GST	Sf9 insect cells using baculovirus	Full Length	NM_139276
STAT3 Protein	S54-54BH	Human	HIS	Sf9 insect cells using baculovirus	Full Length	NM_139276
STAT4 Protein	S55-54H	Human	HIS	Sf9 insect cells using baculovirus	Full Length	NM_003151
STAT5 Protein	S56-54H	Human	HIS	Sf9 insect cells using baculovirus	Full Length	NM_003152
STAT5B Protein	S56-54BG	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC065227
STAT5B Protein	S56-54BH	Human	HIS	Sf9 insect cells using baculovirus	Full Length	BC065227
STAT6 Protein	S57-30G	Human	GST	Sf9 insect cells using baculovirus	Full Length	BC075852
TDP43 Protein	T74-30G	Human	GST	E.coli	Full Length	NM_007375

GROWTH FACTORS

SignalChem offers a wide range of recombinant cytokines, chemokines and growth factors manufactured under strict quality controls. With high purity and endotoxin levels of ≤ 0.1 ng/ug (IEU/ug), our ligands are perfect candidates to be included in your research and drug discovery projects.

Growth Factors

View all

Chemokines

Name	Catalog Number	Species	Tag	Expression System	Sequence	Genbank Number
IP-10	I838-40N	Human	HIS	E.coli	22-98	NP_001556.2
RANTES (CCL5)	R801-40N	Human		E.coli		AAH08600
SDF-1	S821-40N			E.coli	MKPVSLSYRC PCRFFESHVA RANVKHLKIL NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALN

Cytokines

View all Cytokines

Name	Catalog Number	Species	Tag	Expression System	Sequence	Genbank Number
IFN-gamma	I837-40N	Human		E.coli		NP_000610.2
TNF-alpha	T860-40N	Human		E.coli		P01375
TNF-beta	T861-40BG	Mouse	GST	Sf9 insect cells using baculovirus	34-end	P09225
TNFB	T861-40N	Human		E.coli	35-205	AAH34729.1
TRAIL	T880-40N	Human		E.coli	114-281	NP_003801.1
VEGF-C	V812-40N	Human		E.coli		NP_005420.1

TAU PROTEINS

View All

Tau proteins constitute a family of very important neuronal proteins that bind to the neuronal microtubules. As one of the first discovered and characterized microtubule-associated proteins, the role of Tau proteins has ever since been associated with an array of neuro-biochemical processes including formation and maintenance of neuronal transport system. Researchers have been constantly monitoring the role of myriad Tau proteins in neuronal pathways and their mutagenesis in neurodegenerative disorders such as Alzheimer’s disease.

Abnormal phosphorylation of Tau can lead to deposits of Tau filaments leading to severe pathogenesis in multiple neurodegenerative disorders such as Alzheimer’s disease. Over the years there has been an exponential boost in Tau research after multiple neurodegenerative diseases displayed tangled forms of Tau proteins in the brain. These observations have established Tau proteins as key potential therapeutic targets for neurodegenerative diseases and motivated scientists globally to invest their drug discovery efforts in understanding the Tau proteins and in developing novel therapeutics to modulate their functions.

Signalchem manufactures one of the most comprehensive selections of recombinant Tau proteins in the world. These include various protein isoforms, phospho-Taus, and mutant variants. Our selection of a wide range of Tau proteins demonstrates SignalChem’s drive to provide quality reagents to facilitate research in neurobiology and these proteins have so far been utilized in a variety of fields including neurodegeneration, oncology, epigenetics, cell stress, and more.

Name	Catalog Number	Species	Tag	Expression System	Sequence	Genbank Number
Tau Protein Marker	T08-07N
Tau-316 Protein	T02-54N	Human	TAG-Free	E.coli	Full Length	P10636-3
Tau-352 Protein	T03-54N	Human	TAG-Free	E.coli	Full Length	P10636-2
Tau-381 Protein	T04-54N	Human	TAG-Free	E.coli	Full Length	P10636-4
Tau-383 (N352H) Protein	T05-56N	Human	TAG-Free	E.coli	Full Length (N352H)	P10636-6
Tau-383 Protein	T05-54N	Human	TAG-Free	E.coli	Full Length	P10636-6
Tau-410 Protein	T06-54N	Human	TAG-Free	E.coli	Full Length	P10636-5
Tau-412 Protein	T07-54N	Human	TAG-Free	E.coli	Full Length	P10636-7
Tau-412, GSK3beta-phosphorylated	T07-50CN	Human		E.coli	Full length	P10636-7
Tau-441 (1-391) Protein	T08-55CN	Human	TAG-Free	E.coli	1-391	P10636-8
Tau-441 (1-421) Protein	T08-55BN	Human	TAG-Free	E.coli	1-421	P10636-8
Tau-441 (127-421) Protein	T08-55KN	Human		E.coli	127-421	P10636-8
Tau-441 (151-391) Protein	T08-55FN	Human	TAG-Free	E.coli	151-391	P10636-8
Tau-441 (151-421) Protein	T08-55EN	Human	TAG-Free	E.coli	151-421	P10636-8
Tau-441 (151-441) Protein	T08-55DN	Human	TAG-Free	E.coli	151-441	P10636-8
Tau-441 (216-391) Protein	T08-55LN	Human	TAG-Free	E.coli	216-391	P10636-8
Tau-441 (231-391) Protein	T08-55IN	Human	TAG-Free	E.coli	231-391	P10636-8
Tau-441 (231-421) Protein	T08-55HN	Human	TAG-Free	E.coli	231-421	P10636-8
Tau-441 (231-441) Protein	T08-55GN	Human	TAG-Free	E.coli	231-441	P10636-8
Tau-441 (244-372) Protein	T08-55N	Human	TAG-Free	E.coli	244-372	P10636-8

OTHER PROTEINS

Other Proteins

Other Recombinant Protein

Protein G Products

Peptides

COMPOUNDS

View All Enzyme Inhibitors

Compounds such as enzyme activity modulators (activators and inhibitors) are important tools to study an enzyme’s role in key cell signaling pathways and enzymatic reactions. SignalChem offers one of the most comprehensive ranges of biologically active recombinant enzymes as well as a broad range of high- quality/low-cost enzyme activators and inhibitors of different classes of enzymes including protein kinases, phosphatases, and epigenetic enzymes. These compounds would enable scientists to study cell signaling pathways and facilitate enzyme research and drug discovery projects.

Kinase Inhibitors

View All Kinase Inhibitors

Name	Catalog Number	CAS Registration Number	Molecular Formula	Purity	Chemical Name
6BIO	G08-900	667463-62-9	C16H10BrN3O2	98% (TLC: 5% CH3OH3 in CH2Cl2, Rf=0.43); NMR (Conforms)	(2’Z,3’E)-6-Bromoindirubin -3′-oxime
7-CL-O-Nec-1	R07-901B	852391-15-2	C13H12ClN3O	>98% (TLC); NMR (Conforms)	Nec-1s; 5-((7-Chloro-1H-indol -3-yl)methyl)-3-methylimidazolidine-2,4-dione
A83-01	A09-900	909910-43-6	C25H19N5S	>98% (TLC); NMR (Conforms)	3-(6-Methyl-2-pyridinyl)-N-phenyl -4-(4-quinolinyl)-1H-pyrazole-1-carbothioamide
AAL-993	F11-900C	269390-77-4	C20H16F3N3O	>98% (TLC); NMR (Conforms)	2-((4-Pyridyl)methyl)amino-N-(3 (trifluoromethyl)phenyl)benzamide
AG-1296	P12-900	146535-11-7	C16H14N2O2	>98% (TLC); NMR (Conforms)	6,7-Dimethoxy-3-phenyl quinoxaline
AG-1478	E10-900B	175178-82-2	C16H14ClN3O2	>98% (TLC); NMR (Conforms)	1. N-(3-Chlorophenyl)-6,7- dimethoxy-4-quinazolinanine
AG-1517	K11-900B	153436-54-5	C16H14BrN3O2 ∙ HCl	>98% (TLC); NMR (Conforms)	1. 4-(3-Bromoanilino)-6,7- dimethoxyquinazoline hydrochloride
AG-490	J02-901	133550-30-8	C17H14N2O3	>98% (TLC); NMR (Conforms)	(E)-2-Cyano-3-(3,4-dihydrophenyl) -N-(phenylmethyl)-2-propenamide
AG-879	N28-909B	148741-30-4	C18H24N2OS	>99% (TLC); NMR (Conforms)	alpha-Cyano-(3,5-di-t-butyl-4-hydroxy) thiocinnamide
Alsterpaullone	G09-901C	237430-03-4	C16H11N3O3	>96% (TLC); NMR (Conforms)	9-Nitro-7,12-dihydroindolo-[3,2-d] [1] benzazepin-6(5)-one
Amlexanox	T02-901	68302-57-8	C16H14N2O4	>98% (TLC); NMR (Conforms)	2-Amino-7-(1-methylethyl)-5-oxo-5H- [1]benzopyrano[2,3-b]pyridine-3-carboxylic acid
Apigenin	K10-900C	520-36-5	C15H10O5	>98% (TLC); NMR (Conforms)	4’,5,7-Trihydroxyflavone
AR-A014418	G09-901	487021-52-3	C12H12N4O4S	>98% (TLC); NMR (Conforms)	N-[(4-Methoxyphenyl) methyl]-N’-(5-nitro-2-thiazolyl) urea
AS-041164	P29-901	6318-41-8	C11H7NO4S	>98% (TLC); NMR (Conforms)	1. 5-Benzo[1,3]dioxol-5- ylmethylene-thiazolidine-2,4-dione
BAY 11-7082	C51-900	19542-67-7	C10H9NO2S	>98% (TLC); NMR (Conforms)	(E)-3-[(4-methylphenylsulfonyl] -2-propenenitrile
BML-259	C33-901	267654-00-2	C14H16N2OS	>98% (TLC: 5% CH3OH/CH2Cl2; Rf=0.46); NMR (Conforms)	N-(5-Isopropylthiazol-2-yl) phenylacetamide
BML-277	C48-901	516480-79-8	C20H14ClN3O2	≥98% (TLC; 1:1 ethylacetate: hexane; Rf=0.17); NMR (Conforms)	1. 2-(4-(4-chlorophenoxy)phenyl)- 1H-benzimidazole-5-carboxamide
BRD-7389	R15-901	376382-11-5	C24H18N2O2	>98% (TLC); NMR (Conforms)	1-[(2-Phenylethyl)amino]-3H-naphtho [1,2,3-de]quinoline-2,7-dione
CHIR-99021	G09-901B	252917-06-9	C22H18Cl2N8	>98% (TLC); NMR (Conforms)	6-[[2-[[4-(2,4-Dichlorophenyl)-5- (5-methyl-1H-imidazol-2-yl)-2-pyrimidinyl] amino] ethyl] amino]-3-pyridinecarbonitrile
CID-2011756	P72-901	638156-11-3	C22H21ClN2O3	≥98% (TLC); NMR (Conforms)	5-(3-Chlorophenyl)-N-[4-(4-morpholinylmethyl) phenyl]-2-furancarboxamide

Phosphatase Inhibitors

View All Phosphatase Inhibitors

Name	Catalog Number	CAS Registration Number	Molecular Formula	Purity	Chemical Name
Cantharidin	P16-902C	56-25-7	C10H12O4	>98% (HPLC)	3α,7α-Dimethylhexahydro-4,7- epoxyisobenzofuran-1,3-dione
NSC-663284	C04-902	383907-43-5	C15H16ClN3O3	>98%	6-Chloro-7-[[2-(4-morpholinyl) ethyl]amino]-5,8-quinolinedione
Okadaic acid, free acid	P16-902	78111-17-8	C44H68O13	>98% (TLC)	None

Epigenetic Enzyme Inhibitors

Name	Catalog Number	CAS Registration Number	Molecular Formula	Purity	Chemical Name
2’-Deoxy-5-fluorocytidine	D353-904D	10356-76-0	C9H12FN3O4	>98%	5-Fluoro-2’-deoxycytidine; FCDR; FdCyd; NSC-48006
5-Azacytidine	D353-904	320-67-2	C8H12N4O5	>98% (TLC)	4-Amino-1-(beta-D-ribofuranosyl)- 1,3,5-triazin-2(1H)-one, Ladakamycin
AK-7	S36-904	420831-40-9	C19H21BrN2O3S	98% (TLC)	N-(3-Bromophenyl)-3-[(hexahydro-1H -azepin-1-yl)sulfonyl]benzamide
APICIDIN	H83-904B	183506-66-3	C34H49N5O6	≥98%	Cyclo[(2S)-2-Amino-8-oxodecanoyl-1- methoxy-L-tryptophyl-L-isoleucyl-(2R)-2-piperidinecarbonyl]
BIX-01294 3HCl	S344-903	935693-62-2	C28H38N6O2.3HCl	>98%	2-(Hexahydro-4-methyl-1H-1,4-diazepin-1-yl) -6,7-dimethoxy-N-[1-(phenylmethyl)-4-piperidinyl] -4-quinazolinamine trihydrochloride
BML-210	H83-904C	537034-17-6	C20H25N3O2	98%	N1-(2-aminophenyl)-N8-phenyloctanediamide
C-646	K311-903	328968-36-1	C24H19N3O6	>98%	4-[4-[[5-(4,5-Dimethyl-2-nitrophenyl)-2-furanyl] methylene]-4,5-dihydro-3-methyl-5-oxo-1H-pyrazol-1-yl]benzoic acid
Chaetocin	K421-904C	28097-03-2	C30H28N6O6S4	>99%	Lys methyltransferase inhibitor
CPTH2	K311-903B	357649-93-5	C14H14ClN3S	>98% (TLC)	Cyclopentylidene-[4-(4’-chlorophenyl)thiazol-2-yl]hydrazone
CUDC-101	H83-904	1012054-59-9	C24H26N4O4	>99% (HPLC)	7-((4-((3-ethynylphenyl)amino)-7-methoxyquinazolin -6-yl)oxy)-N-hydroxyheptanamide
Daminozide	K421-904B	1596-84-5	C6H12N2O3	98%	1-(2,2-Dimethylhydrazide)butanedioic acid
Decitabine	D353-904B	2353-33-5	C8H12N4O4	>98%	5-Aza-2’-deoxycytidine; NSC-127716
DMOG	J48-904	89464-63-1	C6H9NO5	>98%	N-(2-Methoxy-2-oxoacetyl)glycine methyl ester; Dimethyloxalylglycine
EX-527	S35-904F	49843-98-3	C13H13ClN2O	98% (TLC)	6-Chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamide (racemic)
Garcinol	K311-903C	78824-30-3	C38H50O6	>97% (TLC)	Camboginol, 3-(3,4-dihydroxybenzoyl)-4-hydroxy-8,8-dimethyl- 1,7-bis(3-methyl-2-buten-1-yl)-5-[(2S)-5-methyl-2- (1-methylethenyl)-4-hexen-1-yl]- bicyclo[3.3.1]non-3-ene-2,9-dione
GSK-J1 (Free acid)	J48-904B	1373422-53-7	C22H23N5O2	99%	N-[2-(2-Pyridinyl)-6-(1,2,4,5-tetrahydro-3H-3- benzazepin-3-yl)-4-pyrimidinyl]-β-alanine
GSK-J4 (ethylester)	J48-904C	1373423-53-0	C24H27N5O2	>99%	N-[2-(2-Pyridinyl)-6-(1,2,4,5-tetrahydro-3H-3- benzazepin-3-yl)-4-pyrimidinyl]-β-alanine ethyl ester
HPA (HEXYL-4-PENTYNOIC ACID)	H83-904D	96017-59-3	C11H18O2	>97% (TLC)	2-(2-propynyl)octanoic acid (racemic); HPA
IOX1 (8H5QCA)	J48-904D	5852-78-8	C10H7NO3	>98% (HPLC)	5-Carboxy-8-hydroxyquinoline, 8-Hydroxy-5-quinolinecarboxylic acid
ML-324	J48-904E	1222800-79-4	C21H23N3O2	>98% (TLC)	N-(3-(dimethylamino)propyl)-4-(8-hydroxyquinolin-6-yl)benzamide

Epigenetic Enzyme Activators

Name	Catalog Number	CAS Registration Number	Molecular Formula	Purity	Chemical Name
BML-278	S35-904C	N/F	C24H25NO4	>98% (TLC)	N-Benzyl-3,5-dicarbethoxy-4-phenyl-1,4-dihydropyridine
CTPB	P07-903	586976-24-1	C31H43ClF3NO2	98%	N-(4-chloro-3-trifluoromethyl-phenyl) -2-ethoxy-6-pentadecyl-benzamide
Resveratrol	S35-904	501-36-0	C14H12O3	>98%	3,4’,5-Trihydroxy-trans-stilbene; 5-[(1E)-2- (4-Hydroxyphenyl)ethenyl]-1,3,benzenediol
SirtAct	S35-904E	839699-72-8	C20H25N5O2	98% (TLC)	2-Amino-N-cyclopentyl-1-(3-methoxypropyl) -1H-pyrrolo[2,3-b]quinoxaline-3-carboxamide; CAY10591

Kinase Activators

Name	Catalog Number	CAS Registration Number	Molecular Formula	Purity	Chemical Name
8-pCPT-2-O-Me-cAMP-AM	R46-905	1152197-23-3	C20H21ClN5O8PS	>97% (HPLC)	8-(4-Chlorophenylthio)-2′-O-methyladenosine-3’, 5′-cyclic monophosphate acetoxymethyl ester
AICAR	P47-900B	2627-69-2	C9H14N4O5	>99% (TLC); NMR (Conforms)	1. 5-Aminoimidazole-4-carboxamide 1-B-D-ribofuranoside 2. Acadesine
MHY-1485	F17-901B	326914-06-1	C17H21N7O4	>98% (TLC); NMR (conforms)	4,6-Di-4-morpholinyl-N-(4-nitrophenyl)-1,3,5-triazin-2-amine
SC-79	A16-901	305834-79-1	C17H17ClN2O5	98% (TLC); NMR (Conforms)	2-Amino-6-chloro-α-cyano-3-(ethoxycarbonyl) -4H-1-benzopyran-4-acetic acid, ethyl ester

Reagents

Enzyme Substrates

Other Biochemicals

ASSAY BUFFER AND CO-FACTORS

View All Assay Buffer and Co-factors

View All Assay Buffer

Bioreagents are crucial components in regular laboratory applications like enzyme assays. Without high-quality reagents, it is impossible to generate desired assay results, hampering important disease research and drug discovery projects. Our scientific team has over 100 years of combined research and industrial experience in the recombinant proteins and bioreagent production.

SignalChem’s expertise allows us to understand the importance of the quality of assay reagents and substrates in research and our goal is to provide highest-quality bioreagents to the global scientific community. We offer high quality and cost-effective bioreagents such as assay reagents, peptide substrates, oligo substrates, lipid substrates, and more.

Name	Catalog Number	Application
Acetyltransferase Assay Buffer	A01-09	Acetyltransferase Assay
Acetyltransferase Dilution Buffer	A21-09	Acetyltransferase Assay
AMP Solution	A46-09	Kinase Assay
ATP Stock Solution	A50-09	Kinase Assay
Ca2+/Calmodulin Solution II, 10x	C02-39B	Kinase Assay
CaCl2 Solution	C02-09	Enzyme Assay
cAMP Solution	A47-09	Enzyme Assay
cGMP Solution	G47-09	Enzyme Assay
DTT Solution	D86-09B
DTT Solution	D86-09
DUB Reaction Buffer (4X)	D01-09
Fructose Kinase Assay Buffer I	F01-09	Enzyme Assay
Fructose Kinase Dilution Buffer I	F21-09	Enzyme Assay
GTP Solution	G50-09	Enzyme Assay
GTP Solution II	G50-09B	Enzyme Assay
Kinase Assay Buffer I	K01-09	Kinase Assay
Kinase Assay Buffer II	K02-09	Kinase Assay
Kinase Assay Buffer III	K03-09
Kinase Dilution Buffer I	K21-09	Kinase Assay
Kinase Dilution Buffer II	K22-09	Kinase Assay

ASSAY SUBSTRATES

Bioreagents are crucial components in regular laboratory applications like enzyme assays. It is essential to use high-quality bioreagents to develop reliable assay methods and to generate reproducible and good-quality assay results. Our scientific team has over 100 years of combined research and industrial experience in the production of recombinant proteins and bioreagents. We leverage our expertise and experience to provide the global scientific community with the high quality and cost-effective bioreagents such as assay reagents, peptide substrates, oligo substrates, lipid substrates, siRNA reagents and more.

Assay Substrates

Lipid Substrates

Name	Catalog Number
DLG (Dilauroyl-sn-glycerol)	D430-59
PI (Phosphatidylinositol)	P420-59
PI(3)P	P421-59
PI(3)P:PS	P426-59
PI(4)P	P422-59
PI(4)P:PS	P427-59
PI(4,5)P2	P424-59
PI(4,5)P2:PS	P429-59
PI(5)P	P423-59
PI(5)P:PS	P428-59
PI:PS	P425-59B

Oligo Substrates

Name	Catalog Number	Application
DNMT Substrate-1	D53-58	DNA methyltransferase assay (50pmole per reaction).
METTL3/METTL14 Substrate	M323-58	A methyltransferase METTL3/METTL14 complex assay.
Poly (1:1 dI, dC) Acid	P62-58	DNA methyltransferase assay (0.25ug per reaction).

Peptide Substrates

Substrate Peptides

Name	Catalog Number	Application
Abltide	A02-58	Kinase Assay
Acetylated Histone H3 Peptide I	H12-358	Acetylation/Methylation Assays
Acetylated Histone H4 Peptide I	H13-358	Acetylation/Methylation Assays
AKT (PKB) Substrate	A05-58	Kinase Assay
AKT (SGK) Substrate	A08-58	Kinase Assay
AMARA Peptide	A11-58	Kinase Assay
Amyloid beta (1-11) Peptide	A06-558B
Amyloid beta (1-15) Peptide	A06-558I
Amyloid beta (11-20) Peptide	A06-558K
Amyloid beta (11-40) Peptide	A06-558G
Amyloid beta (11-40), pyrE	A06-558H
Amyloid beta (11-42) Peptide	A06-558W
Amyloid beta (11-42), pyrE	A06-558X
Amyloid beta (12-28) Peptide	A06-558L
Amyloid beta (17-28) Peptide	A06-558M
Amyloid beta (25-35), C-terminal-amide	A06-558O
Amyloid beta (25-35), C-terminal-OH	A06-558N
Amyloid beta (25-35), N-Alpha-Biot-LC Labeled	A06-558Q
Amyloid beta (25-35), Scrambled	A06-558P
Amyloid beta (29-40) Peptide	A06-558R

Protein Substrates

Name	Catalog Number	Application
ARPP19 Protein	A76-54G	Western Blot
Casein Protein	C03-54N	Kinase Assay, Western Blot
Casein, Dephosphorylated	C03-54BN	Kinase Assay, Western Blot
DUB Substrate I	U06-57
DUB Substrate II	S293-57
DUB Substrate III	N263-57
Histone H1 Protein	H10-54N	Kinase Assay, Western Blot
Histone H3 Protein	H12-54N	Kinase Assay, Western Blot
LC20 Protein	M89-54G	Kinase Assay, Western Blot
MBP Protein	M42-51N	Kinase Assay, Western Blot
Moesin Protein	M52-55G	Kinase Assay, Western Blot
PDHA1 Protein	P57-55H	Kinase Assay, Western Blot
RanGAP1 Protein	R298-31H	Western Blot

Carbohydrate Substrate

Name	Catalog Number	Application
D-Fructose-6-phosphate	F520-59	PFKFB Assay
D-Glucose-6-phosphate	F521-59	PFKFB Assay

siRNAs

siRNA Reagents

siRNA Sets

Name	Catalog Number	Formulation	GenBank ID	Specificity
14-3-3 alpha/beta siRNA Set I	Y71-911	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	NM_003404	14-3-3 alpha/beta siRNAs are designed to specifically knock-down human 14-3-3 alpha/beta expression.
14-3-3 epsilon siRNA Set I	Y75-911	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	NM_006761	14-3-3 epsilon siRNAs are designed to specifically knock-down human 14-3-3 epsilon expression.
14-3-3 sigma siRNA Set I	Y86-911	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	NM_006142	14-3-3 sigma siRNAs are designed to specifically knock-down human 14-3-3 sigma expression.
14-3-3 theta siRNA Set I	Y84-911	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	NM_006826	14-3-3 theta siRNAs are designed to specifically knock-down human 14-3-3 theta expression.
14-3-3 zeta siRNA Set I	Y92-911	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	NM_003406	14-3-3 zeta siRNAs are designed to specifically knock-down human 14-3-3 zeta expression.
4EBP1 siRNA Set I	E35-911	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	NM_004095	4EBP1 siRNAs are designed to specifically knock-down human 4EBP1 expression.
AATK siRNA Set I	A02-911	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	NM_007377	AATK siRNAs are designed to specifically knock-down human AATK expression.
ABL1 siRNA Set I	A03-911	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	NM_009594	ABL1 siRNAs are designed to specifically knock-down human ABL1 expression.
ABL2 siRNA Set I	A04-911	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	NM_005158	ABL2 siRNAs are designed to specifically knock-down human ABL2 expression.
ACK siRNA Set I	A05-911	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	NM_005781	ACK siRNAs are designed to specifically knock-down human ACK expression.
ACVR2A siRNA Set I	A35-911	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	NM_001616	ACVR2A siRNAs are designed to specifically knock-down human ACVR2A expression.
ACVR2B siRNA Set I	A36-911	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	NM_001106	ACVR2B siRNAs are designed to specifically knock-down human ACVR2B expression.
ADD1 siRNA Set I	A38-911	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	NM_001119	ADD1 siRNAs are designed to specifically knock-down human ADD1 expression.
AFX siRNA Set I	A42-911	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	NM_001170931	AFX siRNAs are designed to specifically knock-down human AFX expression.
AKT1/PKB alpha siRNA Set I	A16-911	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	NM_005163	AKT1/PKB alpha siRNAs are designed to specifically knock-down human AKT1/PKB alpha expression.
AKT2/PKB beta siRNA Set I	A17-911	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	NM_001626	AKT2/PKB beta siRNAs are designed to specifically knock-down human AKT2/PKB beta expression.
AKT3 siRNA Set I	A18-911	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	NM_005465	AKT3 siRNAs are designed to specifically knock-down human AKT3 expression.
ALK1 siRNA Set I	A09-911	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	NM_000020	ALK1 siRNAs are designed to specifically knock-down human ALK1 expression.
ALK2 siRNA Set I	A06-911	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	NM_001105	ALK2 siRNAs are designed to specifically knock-down human ALK2 expression.
ALK3 (BMPR1A) siRNA Set I	B04-911	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	NM_004329	ALK3 (BMPR1A) siRNAs are designed to specifically knock-down human ALK3 (BMPR1A) expression.

siRNA Controls

Name	Catalog Number	Formulation	Purity	Sequence	Specificity
beta-Actin Positive Control siRNA	A03-911D	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	Single PAGE band	5’-UGA AGA UCA AGA UCA UUG CdTdT-3’ and 5’-GCA AUG AUC UUG AUC UUC AdTdT-3’	beta-Actin Positive Control siRNA is designed to specifically knock-down human beta-Actin expression.
Cy3 Labeled Negative Control siRNA	S02-910N	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	Cy3 Negative Control siRNA duplex resolves as a single band via PAGE	5’-UUC UCC GAA CGU GUC ACG UTT-Cy3-3’ and 5’- ACG UGA CAC GUU CGG AGA ATT-3’
FAM Labeled Negative Control siRNA	S03-910N	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	≥ 90% by HPLC	5’-UUC UCC GAA CGU GUC ACG UTT-FAM-3’ and 5’- ACG UGA CAC GUU CGG AGA ATT-3’
GAPDH Positive Control siRNA	G13-911B	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	Single PAGE band	5’-GUG GAG AUU GUU GCC AUC AdTdT-3’ and 5’- UGA UGG CAA CAA UCU CCA CdTdT-3’	GAPDH Positive Control siRNA is designed to specifically knock-down human GAPDH expression.
Unlabeled Negative Control siRNA	S01-910N	The siRNAs are supplied as a lyophilized powder and shipped at room temperature.	≥ 90% by HPLC	5’-UUC UCC GAA CGU GUC ACG UTT-3’ and 5’-ACG UGA CAC GUU CGG AGA ATT-3’

IVD Products

ALLERGENS

Allergens are antigens (mostly proteins) produced by food, animals, insects, and vegetation, which if inhaled or eaten can trigger a strong immune response by the immune system causing allergic reactions. These proteins are usually non-toxic, but have a tendency to unintentionally trigger a severe immune response in certain individuals causing allergic disorders; which can damage tissues and vital organs. In technical terms, allergens are antigens, which upon identification by the immune system cause immunoglobulin E mediated type-1 hypersensitivity or allergic disorders in atopic individuals. SignalChem has developed an extensive portfolio of bioallergens from various animal, plant, mold, and food sources following strict quality systems and manufacturing protocols intended for in vitro diagnosis and research studies. We aim to provide high-quality recombinant allergens to the global scientific community to aid the development of kits/tests for better diagnosis of IgE mediated allergies and related therapeutic interventions. Our allergens are excellent candidates for use in in-vitro studies of antigen presentation, T cell functions & research, IgE response, innate immune response, and can expedite the development of improved antibody detection kits, histamine assays, and allergy response related biological assays.

Name	Catalog Number	Species	Expression System	Sequence	Genbank Number
Amb a 1	AA01-A921H	Ambrosia artemisiifolia	Plant	Full length	M80558.1
Art v 1	AV01-A921H	Artemisia vulgaris	Plant	Full length	AF493943.1
Art v 3	AV03-A921H	Artemisia vulgaris	Plant	Full length	EU564845.1
Art v 3.02	AV02-A921H	Artemisia vulgaris	E.coli	Full length	EU564845
Asp f 3	AF03-A931H	Aspergillus fumigatus	E.coli	Full length	O43099
Asp f 6	AF06-A931H	Aspergillus fumigatus	E.coli	Full length	Q92450
Asp f 9	AF09-A931H	Aspergillus fumigatus	E.coli	Full length	Q8J0P4 (O42800)
Can f 1	CF01-A951H	Canis familiaris	E.coli	Full length	AF027177
Can f 2	CF02-A951H	Canis familiaris	E.coli	Full length	AF027178.1
Can f 3	CF03-A951H	Canis familiaris	E.coli	Full length	NM_001003026.1
Der f 1	DF01-A941H	Dermatophagoides farinae	E.coli	Full length	Q3HWZ4
Der f 2	DF02-A941H	Dermatophagoides farina	E.coli	Full length	Q00855
Der p 2	DP02-A941H	Dermatophagoides pteronyssinus	E.coli	Full length	P49278
Der p 21	DP21-A941H	Dermatophagoides pteronyssinus	E.coli	Full length	DQ354124.1
Der p 21/23	DP22-A941H	Dermatophagoides pteronyssinus	E.coli	Full length	Q2L7C5
Der p 7	DP07-A941H	Dermatophagoides pteronyssinus	E.coli	Full length	P49273
Fel d 1	FD01-A951H	Felis catus	E.coli	Full length	NM_001048153
Fel d 4	FD04-A951H	Felis catus	E.coli	Full length	NM_001009233
Man i 3	MN03-A921H	Mangifera indica	Plant	Full length	DQ270547.1

ISOTHERMAL AMPLIFICATION-RELATED PROTEINS

Isothermal amplification of nucleic acids is a biochemical reaction that rapidly and efficiently accumulates nucleic acid sequences at a constant temperature. Isothermal amplification techniques have been developed as alternatives to polymerase chain reaction (PCR), which requires sophisticated thermocycler equipment. Because of their simplicity, these techniques are particularly useful in diagnostic applications and have been used for biosensing targets such as DNA, RNA, cells, proteins, small molecules, and ions. Isothermal amplification reactions usually require the concerted actions of multiple protein components and nucleic acid sequence, such as DNA binding proteins, recombinase and polymerase.

Name	Catalog Number	Species	Tag	Expression System	Sequence	Genbank Number
Bsu DNA Polymerase Large Fragment	BD01-E311U	Bacillus subtilis	Tag-free	E.coli		AF008220
Sau DNA Polymerase Large Fragment	SD01-E311U	Staphylococcus aureus	Tag-free	E.coli		CP076105
T4 gp32 Protein	T4G32-C501H	Escherichia virus T4	HIS	E.coli	Full length	J02513
T4 UvsX DNA Recombinase	T4SX-E311U	Escherichia virus T4	Tag-free	E.coli	Full length	P04529
T4 UvsY Protein	T4SY-C501H	Escherichia virus T4	HIS	E.coli	Full length	X04856

CRISPR ASSOCIATED PROTEINS

Clustered regularly interspaced short palindromic repeats (CRISPRs) and their CRISPR-associated (Cas) proteins constitute the CRISPR-Cas immune system, which provides adaptive immunity against invasive elements such as viruses and plasmids in bacteria and archaea. Cas proteins are endonucleases that use a single guide RNA (sgRNA) to form complementary base pairs with target gene and then cleave the target at specific sites. Due to the programmability, high editing efficiency and ease of use , the CRISPR-Cas systems have emerged as a powerful tool for gene manipulation, with the type II CRISPR-Cas9 system from Streptococcus pyogenes already being widely adopted for various genome-targeting purposes. A distinct group of Cas proteins, including Cas12 and Cas13 among others, possess “collateral cleavage” activity that have been exploited and demonstrated in various diagnostic technologies.

Name	Catalog Number	Species	Tag	Expression System	Sequence	Genbank Number
AapCas12b, Active	C12B1-E311U	Alicyclobacillus acidiphilus		E.coli		WP_067623834
AsCas12a, Active	CS12A-E311H	Acidaminococcus	HIS	E.coli		WP_021736722
Cas12 (LbCpf1), Active	C12CR-E241G	Lachnospiraceae	GST	E.coli	1-1227	ATB19154
Cas12 (LbCpf1), Active	C12CR-E241H	Lachnospiraceae	HIS	E.coli	1-1227	ATB19154
Cas13a (LwC2c2), Active	CS13A-E321H	Leptotrichia wadei	HIS	E.coli	1-1152	WP_021746774
LbuCas13a, Active	CS13A-E322U	Leptotrichia buccalis		E.coli		WP_015770004
TtCsm6, Active	CS06-E311H	Thermus thermophilius	HIS	E.coli		WP_011229148
PsmCas13b	CS13B-E312H	Prevotella sp	HIS	E.coli		WP_028912271
SpCas9, Active	CS09-E311H	Streptococcus pyogenes	HIS	E.coli	Full length	AAK33936

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