Skyang bioscience offers a comprehensive array of next generation genomic modulation tools that are used to facilitate cutting-edge life science research and advance the development of next generation therapeutics.
The company leverages core products and services combined with expertise in advanced lentiviral design and packaging technologies to provide one-stop, complete workflow solutions for gene modulation projects.
SkyangBio offers the highest-quality CRISPR reagents with proven and trusted performance
SkyangBio’s collaboration with Cold Spring Harbor Laboratory created the most potent shRNAs available.
Off-the-shelf cDNA and ORF libraries. Purchase individual plasmids or arrayed libraries.
SkyangBio’s collaboration with Cold Spring Harbor Laboratory created the most potent shRNAs available.
Use our lentiviral cloning vectors to accomplish your specific applications.
Off-the-shelf cDNA and ORF libraries. Purchase individual plasmids or arrayed libraries.
SkyangBio’s collaboration with Cold Spring Harbor Laboratory created the most potent shRNAs available.
SkyangBio’s transEDIT family of CRISPR-Cas9 reagents provide researchers with the tools to make targeted edits to any gene. With our wide selection of lentiviral vectors, it is possible to perform gene editing experiments in nearly any cell type. Additionally, SkyangBio provides the reagents and custom solutions required for CRISPR activation (CRISPRa) and CRISPR interference (CRISPRi) experiments.
CRISPR-Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) is a revolutionary gene editing technology that allows for precise and targeted modifications to an organism’s DNA. The CRISPR-Cas9 system is used to create a double-stranded break (DSB) in the DNA at the target DNA site specified by the gRNA. The Cas9 enzyme cleaves both strands of the DNA at the target site, which triggers the cell’s natural DNA repair machinery.
SkyangBio provides easy access to this technology through a comprehensive offering of lentiviral vector backbones and expert cloning services. One of the critical components of the CRISPR gene editing process is gRNA design. SkyangBio utilizes the CRoatan algorithm developed at Cold Spring Harbor Laboratories which selects gRNAs for optimal specificity, efficiency and target DNA accessibility. Additionally, the breadth of our available vectors provides maximum flexibility to allow for single and dual guide configurations as well as all-in-one vectors comprised of both gRNA and Cas9 expression cassettes.
SkyangBio also specializes in CRISPR-Cas9 systems that can be used for gene activation CRISPRa (dCas9-VPR) and gene interference CRISPRi (dCAS-KRAB) that do not create DNA breaks, but rather use a catalytically inactive Cas9 to target trans-activators or repressors to specific regions of a gene’s promoter.
Single gRNA target gene sets consist of 3 unique gRNA constructs plus a negative control. Choose the ideal lentiviral vector for your gene knockout experiment. All gRNA constructs are 100% sequence-confirmed before shipping.
Constructs are delivered as glycerol stocks or ready-to-use viral particles.
Don’t see your question? Contact Us and we will be happy to help!
SkyangBio uses the CRoatan algorithm to design each gRNA for knockout. After synthesis and cloning, each construct is sequenced-verified and then shipped, or we start packaging viral particles. The gRNA constructs are not functionally validated. For information on the CRoatan algorithm, please refer to the publication in Molecular Cell located in the product information at the bottom of the page.
Our scientists will work with you to help pinpoint the problem, please contact technical@stratech.co.uk
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
You will receive four 2ml tubes. Each tube will contain 600ul of bacterial glycerol stock. Three tubes will be the gRNAs targeting your gene of interest and one tube will be the negative control.
You will receive 100ul of viral particles for each gRNA and the negative control. The 100ul will be split into 4 tubes of 25ul. So, in total, you will receive 16 tubes.
For vectors containing just the gRNA, you will receive 100ul of 1×10^8 TU/ml for each gRNA construct. For vectors containing the gRNA+Cas9, you will receive 100ul of 1×10^6 TU/ml.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio offers clones on Whatman blotting paper. Just contact us at info@stratech.co.uk and let us and we can provide details and a quote.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio archives all constructs we deliver to our clients Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
If you need the designs before placing your order, please contact us at info@stratech.co.uk and let us know what you need, and we can provide those to you.
We recommend researching your cell line to see which promoter may work best. We do have options for different promoters. Just contact us at info@stratech.co.uk to find out what other promoter options are available.
SkyangBio has some select retroviral and AAV vectors that we can use for gRNAs. We can also create the ideal vector for you through our custom vector service. Contact us with your requests at info@stratech.co.uk
Our lentiviral vectors are all 3rd generation lentiviral vectors and can be packaged with 2nd or 3rd generation packaging systems. SkyangBio uses a 2nd generation packaging system to prepare viral particles. We can use 3rd generation plasmids upon request.
SkyangBio uses a functional titer method for determining lentiviral titers. This is a more accurate calculation of active titer particles than physical titer methods.
SkyangBio uses HEK293T or HeLa cells for functional titer calculations. You will need to use this data to perform a relative titer in your cell line.
Dual gRNA target gene sets consist of 3 unique gRNA constructs plus a negative control. Each dual gRNA construct contains 2 different gRNAs designed to target the gene of interest. Choose the ideal lentiviral vector for your gene knockout experiment. All gRNA constructs are 100% sequence-confirmed before shipping.
Constructs are delivered as glycerol stocks or ready-to-use viral particles
Don’t see your question? Contact Us and we will be happy to help!
Please contact us at info@stratech.co.uk and let us know what you are looking for. It is possible we can find your gene using an alias gene symbol or different gene identifier.
Two gRNAs expressed from the same vector, targeting the same gene, has been shown to be more effective in a gene knock out. https://www.cell.com/molecular-cell/fulltext/S1097-2765(17)30464-1
Our scientists will work with you to help pinpoint the problem, please contact technical@stratech.co.uk
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.com and let us know what you need, and we will send you a quote.
You will receive four 2ml tubes. Each tube will contain 600ul bacterial glycerol stock. Three tubes will be the gRNAs targeting your GOI and one tube will be the negative control.
You will receive 100ul of viral particles for each gRNA and the negative control. The 100ul will be split into 4 tubes of 25ul. So, in total, you will receive 16 tubes.
For vectors containing just two gRNAs, you will receive 100ul of 1×10^7 TU/ml for each gRNA construct.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio offers clones on Whatman blotting paper. Just contact us at info@stratech.co.uk and let us and we can provide details and a quote.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio archives all constructs we deliver to our clients. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
We recommend researching your cell line to see which promoter may work best. We do have options for different promoters. Just contact us at info@stratech.co.uk to find out what those other promoter options are.
After placing your order, your designs will be available. If you need the designs before placing your order, please contact us at info@stratech.co.uk and we can provide those to you.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
SkyangBio uses a functional titer method for determining lentiviral titers. This is a more accurate calculation of active titer particles than physical titer methods.
Our lentiviral vectors are all 3rd generation lentiviral vectors and can be packaged with 2nd or 3rd generation packaging systems. SkyangBio uses a 2nd generation packaging system to prepare viral particles. We can use 3rd generation plasmids upon request.
SkyangBio uses HEK293T or HeLa cells for functional titer calculations. You will need to use this data to perform a relative titer in your cell line.
Single gRNA target gene sets consist of 3 unique gRNA constructs plus a negative control. Choose the ideal lentiviral vector for your gene activation experiment. All gRNA constructs are 100% sequence-confirmed before shipping.
Constructs are delivered as glycerol stocks or ready-to-use viral particles
Don’t see your question? Contact Us and we will be happy to help!
Please contact us at info@stratech.co.uk and let us know what you are looking for. It is possible we can find your gene using an alias gene symbol or different gene identifier.
SkyangBio uses the Broad portal to design each gRNA. After synthesis and cloning, each construct is sequenced-verified and then shipped, or we start packaging viral particles. The gRNA constructs are not functionally validated.
First, our scientists will work with you to help pinpoint the problem contact us technical@stratech.co.uk
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
You will receive four 2ml tubes. Each tube will contain 600ul of bacterial glycerol stock. Three tubes will be the gRNAs targeting your GOI and one tube will be the negative control.
For vectors containing just the gRNA, you will receive 100ul of 1×10^8 TU/ml for each gRNA construct. For vectors containing the gRNA+Cas9, you will receive 100ul of 1×10^6 TU/ml.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio offers clones on Whatman blotting paper. Just contact us at info@stratech.co.uk and let us and we can provide details and a quote.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio archives all constructs we deliver to our clients. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
If you need the designs before placing your order, please contact us at info@stratech.co.uk and we can provide those to you.
We recommend researching your cell line to see which promoter may work best. We do have options for different promoters. Just contact us at info@stratech.co.uk to find out what those other promoter options are.
SkyangBio has some select retroviral and AAV vectors that we can use for gRNAs. We can also create the ideal vector for you through our custom vector service. Contact us with your requests at info@stratech.co.uk.
SkyangBio uses a functional titer method for determining lentiviral titers. This is a more accurate calculation of active titer particles than physical titer methods.
Single gRNA target gene sets consist of 3 unique gRNA constructs plus a negative control. Choose the ideal lentiviral vector for your gene interference experiment. All gRNA constructs are 100% sequence-confirmed before shipping.
Constructs are delivered as glycerol stocks or ready-to-use viral particles
Don’t see your question? Contact Us and we will be happy to help!
Please contact us at info@stratech.co.uk and let us know what you are looking for. It is possible we can find your gene using an alias gene symbol or different gene identifier.
SkyangBio uses the Broad portal to design each gRNA. After synthesis and cloning, each construct is sequenced-verified and then shipped, or we start packaging viral particles. The gRNA constructs are not functionally validated.
First, our scientists will work with you to help pinpoint the problem contact us technical@stratech.co.uk
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
You will receive four 2ml tubes. Each tube will contain 600ul of bacterial glycerol stock. Three tubes will be the gRNAs targeting your GOI and one tube will be the negative control.
For vectors containing just the gRNA, you will receive 100ul of 1×10^8 TU/ml for each gRNA construct. For vectors containing the gRNA+Cas9, you will receive 100ul of 1×10^6 TU/ml.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio offers clones on Whatman blotting paper. Just contact us at info@stratech.co.uk and let us and we can provide details and a quote.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio archives all constructs we deliver to our clients. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
If you need the designs before placing your order, please contact us at info@stratech.co.uk and we can provide those to you.
We recommend researching your cell line to see which promoter may work best. We do have options for different promoters. Just contact us at info@stratech.co.uk to find out what those other promoter options are.
SkyangBio has some select retroviral and AAV vectors that we can use for gRNAs. We can also create the ideal vector for you through our custom vector service. Contact us with your requests at info@stratech.co.uk.
SkyangBio uses a functional titer method for determining lentiviral titers. This is a more accurate calculation of active titer particles than physical titer methods.
SkyangBio offers Cas9 and dCas9 in a variety of lentiviral vector configurations for your CRISPR, CRISPRa and CRISPRi research.
SkyangBio provides Streptococcus pyogenes (SpCas9) for CRISPR gene editing. Once expressed, the nuclease binds to gRNA to form a complex which recognizes a specific genomic target sequence and induces a double-stranded break.
dCas9-VPR fuses a tripartite complex (VPR) with nuclease-inactive Cas9 (dCas9) which is unable to perform DNA cleavage. The VPR domain consists of three transcriptional activators (Vp64, p65, and Rta) thus resulting in targeted gene activation rather than gene editing when directed (via gRNA) to a target site near a promoter region of a gene. The dCas9-VPR system offers a combination of robust and potent gene activation along with ease of use compared to other systems.
dCas9-KRAB fuses the transcriptional repression KRAB domain (Krüppel-associated box) to deactivated Cas9 (dCas9). When dCas9-KRAB is directed to a promoter region of a target gene via a gRNA molecule the KRAB domain recruits co-repressor proteins, which interfere with the transcriptional machinery and results in the strong repression of gene expression.
Constructs are delivered as glycerol stocks or ready-to-use viral particles.
| Format | Promoter | Selection Marker | Product Code |
| Glycerol Stock | EFS | Blasticidin | TECC1002 |
| EFS | Hygromycin | TECC1013 | |
| EFS | Puromycin | TECC1001 | |
| hCMV | Blasticidin | TECC1006 | |
| hCMV | Hygromycin | TECC1014 | |
| hCMV | Puromycin | TECC1005 | |
| Lentiviral Particles (100ul 10^7 TU/ml) | EFS | Blasticidin | TECCV1002 |
| EFS | Hygromycin | TECCV1013 | |
| EFS | Puromycin | TECCV1001 | |
| hCMV | Blasticidin | TECCV1006 | |
| hCMV | Hygromycin | TECCV1014 | |
| hCMV | Puromycin | TECCV1005 |
| Format | Promoter | Selection Marker | Product Code |
| Glycerol Stock | EFS | Blasticidin | TACC1002 |
| EFS | Hygromycin | TACC1009 | |
| EFS | Puromycin | TACC1001 | |
| hCMV | Blasticidin | TACC1006 | |
| hCMV | Hygromycin | Not Available | |
| hCMV | Puromycin | TACC1005 | |
| Lentiviral Particles (100ul 10^7 TU/ml) | EFS | Blasticidin | TACCV1002 |
| EFS | Hygromycin | TACCV1009 | |
| EFS | Puromycin | TACCV1001 | |
| hCMV | Blasticidin | TACCV1006 | |
| hCMV | Hygromycin | Not Available | |
| hCMV | Puromycin | TACCV1005 |
| Format | Promoter | Selection Marker | Product Code |
| Glycerol Stock | EFS | Blasticidin | TICC1002 |
| EFS | Hygromycin | TICC1009 | |
| EFS | Puromycin | TICC1001 | |
| hCMV | Blasticidin | TICC1006 | |
| hCMV | Hygromycin | Not Available | |
| hCMV | Puromycin | TICC1005 | |
| Lentiviral Particles (100ul 10^7 TU/ml) | EFS | Blasticidin | TICCV1002 |
| EFS | Hygromycin | TICCV1009 | |
| EFS | Puromycin | TICCV1001 | |
| hCMV | Blasticidin | TICCV1006 | |
| hCMV | Hygromycin | Not Available | |
| hCMV | Puromycin | TICCV1005 |
Don’t see your question? Contact Us and we will be happy to help!
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
You will receive 100ul of 1×10^7 TU/ml.
You will receive 100ul of viral particles. The 100ul will be split into 4 tubes of 25ul. So, in total, you will receive 4 tubes.
You will receive one 2ml tube. The tube will contain 600ul bacterial glycerol stock.
Yes, SkyangBio offers clones on Whatman blotting paper. Just contact us at info@stratech.co.uk and let us and we can provide details and a quote.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
We recommend researching your cell line to see which promoter may work best. We do have options for different promoters. Just contact us at info@stratech.co.uk to find out what those other promoter options are.
SkyangBio has some select retroviral and AAV that we can use. We can also create the ideal vector for you through our custom vector service. Contact us with your requests at info@stratech.co.uk
SkyangBio uses a functional titer method for determining lentiviral titers. This is a more accurate calculation of active titer particles than physical titer methods.
SkyangBio has popular subsets available ready to ship as glycerol stocks (plasmid DNA and ready-to-use lentiviral particles can also be delivered). Each gene is targeted by 3-4 gRNAs that have been designed using our Croatan algorithm. We also have the ability to create custom arrayed CRISPR subset libraries for any gene list. Learn more about Custom Arrayed Libraries.
Check back often for new additions to our ready to ship arrayed subsets.
| Subset | Species | # of Genes Targeted | # of 96 Well Plate |
| Epigenetic | Human | 406 | 17 |
| Kinase | Human | 906 | 36 |
| Membrane Protein | Human | 830 | 33 |
| Subset | Fomats |
| Epigenetic | Glycerol Stock |
| Plasmid DNA (10ug) | |
| Lentiviral Particles (300ul of 10^7 TU/ml) | |
| Kinase | Glycerol Stock |
| Plasmid DNA (10ug) | |
| Lentiviral Particles (300ul of 10^7 TU/ml) | |
| Membrane Protein | Glycerol Stock |
| Plasmid DNA (10ug) | |
| Lentiviral Particles (300ul of 10^7 TU/ml) |
Don’t see your question? Contact Us and we will be happy to help!
Please check with SkyangBio to see if any other focused subsets are available. You can also provide us with your list of genes to see what gRNAs we have prepared in our inventory: info@statech.co.uk
Yes. Please contact us at info@startech.co.uk for more details.
SkyangBio can provide these subsets in a different vector, but it would be considered a custom arrayed library, where we would clone all of the gRNAs into your vector of choice and would sequence confirm each of the new clones before delivering the new subset. Contact us at info@stratech.co.uk for more details.
SkyangBio’s dual gRNA CRISPR library was developed in collaboration with Cold Spring Harbor Laboratory. The library targets over 18,000 human genes and is the only dual gRNA arrayed library in the industry. The CRISPR library is conveniently delivered as glycerol stock clones in 96-well plate format.
Don’t see your question? Contact Us and we will be happy to help!
Two gRNAs expressed from the same vector, targeting the same gene, has been shown to be more effective in a gene knock out. Please refer to the publication in Molecular Cell located in the product information at the bottom of the page.
SkyangBio used the Croatan algorithm to develop the WG library. Every single dual gRNA construct in the library has been sequenced-verified. Each gRNA construct is not functionally validated but is designed using the rules from gRNAs that were functionally validated.
SkyangBio used the Croatan algorithm to develop the WG library. Every single dual gRNA construct in the library has been sequenced-verified. Each gRNA construct is not functionally validated but is designed using the rules from gRNAs that were functionally validated.
You will receive 670 96-well plates containing ~300ul of bacterial glycerol stock in each well. You will also receive the datafile showing what is in each well.
Yes, SkyangBio can quote to provide an extra copy of each 96-well plate (glycerol stock). Please contact us at info@stratech.co.uk
Yes. SkyangBio can provide a quote for preparing 96-well plates of purified plasmid DNA. Please contact us at info@stratech.co.uk
Yes, just send a list of your genes to info@stratech.co.uk and we will provide you with a quote and additional details.
Yes, just request this information from info@stratech.co.uk and we can provide this to you.
CRISPR dual gRNA knockout human whole genome pooled library
Format
Lentiviral Particles (1ml 10^7)
Format
Plasmid DNA (200ug)
Don’t see your question? Contact Us and we will be happy to help!
Two gRNAs expressed from the same vector, targeting the same gene, has been shown to be more effective in a gene knock out. Please refer to the publication in Molecular Cell located in the product information at the bottom of the page.
Each dual gRNA clone has a unique 25bp barcode cloned into the vector that will be used for detection in NGS. Please contact us for more details at info@stratech.co.uk
You have a choice between plasmid DNA format or lentiviral particle format. Both formats contain a WG pool of the dual gRNA, targeting over 18,000 human genes. The dual gRNAs are QC’d to ensure all gRNAs are properly represented in the pool, so each gRNA has equal chance to interrogate the gene it targets.
The pooled library is usually kept in our inventory and can ship immediately. If this is not the case, please expect a 2–4-week turnaround time.
Yes, just send a list of your genes to info@stratech.co.uk and we will provide you with a quote and additional details.
Yes, just request this information from info@stratech.co.uk and we can provide this to you.
CRISPR single gRNA knockout human whole genome pooled library
Format
Lentiviral Particles (1ml 10^8)
Don’t see your question? Contact Us and we will be happy to help!
Please contact us at info@stratech.co.uk and let us know what you are looking for. It is possible we can find your gene using an alias gene symbol or different gene identifier.
Format
Lentiviral Particles (1ml 10^8)
Don’t see your question? Contact Us and we will be happy to help!
Please contact us at info@stratech.co.uk and let us know what you are looking for. It is possible we can find your gene using an alias gene symbol or different gene identifier.
To help offset the high expense of performing whole genome CRISPR screening experiments, SkyangBio offers our high-quality, whole-genome, amplified CRISPR gRNA oligo pool and cloning vector kit at budget-friendly pricing. Using the two components, pool assembly and viral packaging can then be performed in your lab where you have complete control of the quality benchmarks that are important for your research experiment. Simply choose the cloning vector that suits your needs. We deliver an amplified, whole genome gRNA oligo pool and cloning vector along with detailed protocols for cloning and lentiviral packaging. We make it simple to create your customized CRISPR gRNA knockout library.
| Promoter | Selection Marker | Fluorescent Reporter | Product Code |
| EFS | NONE | NONE | Not available |
| ZsGreen | CPK-H-WG-K-1004 | ||
| mCherry | CPK-H-WG-K-1023 | ||
| Blasticidin | NONE | CPK-H-WG-K-1001 | |
| ZsGreen | CPK-H-WG-K-1013 | ||
| mCherry | CPK-H-WG-K-1025 | ||
| Hygromycin | NONE | CPK-H-WG-K-1002 | |
| ZsGreen | CPK-H-WG-K-1014 | ||
| mCherry | CPK-H-WG-K-1026 | ||
| Puromycin | NONE | CPK-H-WG-K-1003 | |
| ZsGreen | CPK-H-WG-K-1015 | ||
| mCherry | CPK-H-WG-K-1027 | ||
| hCMV | NONE | NONE | Not available |
| ZsGreen | CPK-H-WG-K-1008 | ||
| mCherry | CPK-H-WG-K-1024 | ||
| Blasticidin | NONE | CPK-H-WG-K-1005 | |
| ZsGreen | CPK-H-WG-K-1016 | ||
| mCherry | CPK-H-WG-K-1028 | ||
| Hygromycin | NONE | CPK-H-WG-K-1006 | |
| ZsGreen | CPK-H-WG-K-1017 | ||
| mCherry | CPK-H-WG-K-1029 | ||
| Puromycin | NONE | CPK-H-WG-K-1007 | |
| ZsGreen | CPK-H-WG-K-1018 | ||
| mCherry | CPK-H-WG-K-1030 |
| Promoter | Marker/Reporter | Product Code |
| EFS | Blasticidin | CPK-H-WG-K-1101 |
| Hygromycin | CPK-H-WG-K-1102 | |
| Puromycin | CPK-H-WG-K-1103 | |
| ZsGreen | CPK-H-WG-K-1104 | |
| hCMV | Blasticidin | CPK-H-WG-K-1105 |
| Hygromycin | CPK-H-WG-K-1106 | |
| Puromycin | CPK-H-WG-K-1107 | |
| ZsGreen | CPK-H-WG-K-1108 |
To help offset the high expense of performing whole genome CRISPR screening experiments, SkyangBio offers our high-quality, whole-genome, amplified CRISPR gRNA oligo pool and cloning vector kit at budget-friendly pricing. Using the two components, pool assembly and viral packaging can then be performed in your lab where you have complete control of the quality benchmarks that are important for your research experiment. Simply choose the cloning vector that suits your needs. We deliver an amplified, whole genome gRNA oligo pool and cloning vector along with detailed protocols for cloning and lentiviral packaging. We make it simple to create your customized CRISPR gRNA knockout library.
| Promoter | Selection Marker | Fluorescent Reporter | Product Code |
| EFS | NONE | NONE | Not available |
| ZsGreen | CPK-M-WG-K-1004 | ||
| mCherry | CPK-M-WG-K-1023 | ||
| Blasticidin | NONE | CPK-M-WG-K-1001 | |
| ZsGreen | CPK-M-WG-K-1013 | ||
| mCherry | CPK-M-WG-K-1025 | ||
| Hygromycin | NONE | CPK-M-WG-K-1002 | |
| ZsGreen | CPK-M-WG-K-1014 | ||
| mCherry | CPK-M-WG-K-1026 | ||
| Puromycin | NONE | CPK-M-WG-K-1003 | |
| ZsGreen | CPK-M-WG-K-1015 | ||
| mCherry | CPK-M-WG-K-1027 | ||
| hCMV | NONE | NONE | Not available |
| ZsGreen | CPK-M-WG-K-1008 | ||
| mCherry | CPK-M-WG-K-1024 | ||
| Blasticidin | NONE | CPK-M-WG-K-1005 | |
| ZsGreen | CPK-M-WG-K-1016 | ||
| mCherry | CPK-M-WG-K-1025 | ||
| Hygromycin | NONE | CPK-H-WG-K-1006 | |
| ZsGreen | CPK-H-WG-K-1017 | ||
| mCherry | CPK-H-WG-K-1029 | ||
| Puromycin | NONE | CPK-M-WG-K-1006 | |
| ZsGreen | CPK-M-WG-K-1017 | ||
| mCherry | CPK-M-WG-K-1029 |
| Promoter | Marker/Reporter | Product Code |
| EFS | Blasticidin | CPK-M-WG-K-1101 |
| Hygromycin | CPK-M-WG-K-1102 | |
| Puromycin | CPK-M-WG-K-1103 | |
| ZsGreen | CPK-M-WG-K-1104 | |
| hCMV | Blasticidin | CPK-M-WG-K-1105 |
| Hygromycin | CPK-M-WG-K-1106 | |
| Puromycin | CPK-M-WG-K-1107 | |
| ZsGreen | CPK-M-WG-K-1108 |
With an extensive catalog of constitutive and inducible lentiviral vectors to choose from, SkyangBio offers complete flexibility when planning your shRNA gene knockdown experiment.
RNA interference (RNAi) is the silencing or knockdown of a gene’s expression by specific inactivation of the corresponding mRNA using double-stranded RNA (dsRNA). SkyangBio provides a comprehensive array of tools that enable highly efficient gene knockdown at the transcript level. Our advanced lentiviral based shRNA knockdown vectors utilize a uniquely optimized miR30 scaffold (UltramiR) which maximizes shRNA processing and improves the stability and specificity of the expressed shRNAs. Additionally, our systems utilize RNA polymerase II promoters that promote high expression of long RNAs which allow for multi-cistronic expression cassettes that can include shRNAs and additional reporter genes. Our shRNA designs are created using the shERWOOD algorithm which was developed with collaborators from Cold Spring Harbor Laboratory and predict shRNA’s for maximum potency and can predict rare shRNA designs that are efficient at single copy representation in the genome.
The use of lentiviral vectors to deliver and express shRNAs offers a variety of advantages including stable integration, long-term/permanent expression of the shRNA, and highly efficient and uniform vector delivery (compared to plasmid transfection). We also offer Tet-Inducible shRNA systems to allow complete control over the expression profile and temporal kinetics of shRNA expression.
SkyangBio’s shRNA designs and vectors can also be used to target long non-coding RNAs (lncRNAs) that do not encode for a gene but are involved in key cellular process such as transcriptional regulation, post-transcriptional regulation (e.g. splicing and translation), epigenetic regulation, and nucleation of protein complexes.
shRNA target gene sets consist of 3 unique shRNA constructs plus a negative control. All constructs are 100% sequence-confirmed before shipping. SkyangBio offers an extensive catalog of shRNA lentiviral, inducible lentiviral, and retroviral vectors, allowing for complete flexibility when planning for your gene knockdown experiment.
Constructs are delivered as glycerol stocks or ready-to-use viral particles
pZIP
pLMN
pZIP-TRE3G
pLMP-d
Don’t see your question? Contact Us and we will be happy to help!
Please contact us at info@stratech.co.uk and let us know what you are looking for. It is possible we can find your gene using an alias gene symbol or different gene identifier.
SkyangBio uses the shERWOOD algorithm to design each shRNA. After synthesis and cloning, each construct is sequenced-verified and then shipped. The shRNA constructs are not functionally validated. For information on the shERWOOD algorithm, please refer to the publication in Molecular Cell located in the product information at the bottom of the page.
First, our scientists will work with you to help pinpoint the problem please email technical@stratech.co.uk
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
You will receive four 2ml tubes. Each tube will contain 600ul bacterial glycerol stock. Three tubes will be the shRNAs targeting your gene of interest and one tube will be the negative control.
You will receive 100ul of viral particles for each shRNA and the negative control. The 100ul will be split into 4 tubes of 25ul. So, in total, you will receive 16 tubes.
For constitutive vectors containing the shRNA, you will receive 100ul of 1×10^8 TU/ml for each shRNA construct. For inducible vectors containing the shRNA, you will receive 100ul of 1×10^7 TU/ml.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Although it is not possible to guarantee no “leakiness” of the Tet-On 3G system, it has been shown to have significantly lower basal expression (5- 20-fold) and higher sensitivity to doxycycline (Dox) induction compared to predecessor inducible systems. The extent of basal expression may have some dependence on cell type, particularly in non-mammalian cell lines.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio offers clones on Whatman blotting paper. Just contact us at info@stratech.co.uk and let us and we can provide details and a quote.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio archives all constructs we deliver to our clients. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
If you need the designs before placing your order, please contact us at info@stratech.co.uk and we can provide those to you.
We recommend researching your cell line to see which promoter may work best. We do have options for different promoters. Just contact us at info@stratech.co.uk to find out what those other promoter options are.
SkyangBio has some select AAV vectors that we can use for shRNAs. We can also create the ideal vector for you through our custom vector service. Contact us with your requests at info@stratech.co.uk
SkyangBio uses a functional titer method for determining lentiviral titers. This is a more accurate calculation than physical titer methods.
shRNA sets targeting lncRNA consist of 3 unique shRNA constructs plus a negative control. All constructs are 100% sequence-confirmed before shipping. SkyangBio offers an extensive catalog of shRNA lentiviral, inducible lentiviral, and retroviral vectors to choose from, allowing for complete flexibility when planning for your gene knockdown experiment.
Constructs are delivered as glycerol stocks or ready-to-use viral particles
pZIP
pLMN
pZIP-TRE3G
pLMP-d
Don’t see your question? Contact Us and we will be happy to help!
Please contact us at info@stratech.co.uk and let us know what you are looking for. It is possible we can find your gene using an alias gene symbol or different gene identifier.
SkyangBio uses the shERWOOD algorithm to design each shRNA. After synthesis and cloning, each construct is sequenced-verified and then shipped. The shRNA constructs are not functionally validated. For information on the shERWOOD algorithm, please refer to the publication in Molecular Cell located in the product information at the bottom of the page.
First, our scientists will work with you to help pinpoint the problem please email technical@stratech.co.uk
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
You will receive four 2ml tubes. Each tube will contain 600ul bacterial glycerol stock. Three tubes will be the shRNAs targeting your gene of interest and one tube will be the negative control.
You will receive 100ul of viral particles for each shRNA and the negative control. The 100ul will be split into 4 tubes of 25ul. So, in total, you will receive 16 tubes.
For constitutive vectors containing the shRNA, you will receive 100ul of 1×10^8 TU/ml for each shRNA construct. For inducible vectors containing the shRNA, you will receive 100ul of 1×10^7 TU/ml.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Although it is not possible to guarantee no “leakiness” of the Tet-On 3G system, it has been shown to have significantly lower basal expression (5- 20-fold) and higher sensitivity to doxycycline (Dox) induction compared to predecessor inducible systems. The extent of basal expression may have some dependence on cell type, particularly in non-mammalian cell lines.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio offers clones on Whatman blotting paper. Just contact us at info@stratech.co.uk and let us and we can provide details and a quote.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio archives all constructs we deliver to our clients. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will send you a quote.
If you need the designs before placing your order, please contact us at info@stratech.co.uk and we can provide those to you.
We recommend researching your cell line to see which promoter may work best. We do have options for different promoters. Just contact us at info@stratech.co.uk to find out what those other promoter options are.
SkyangBio has some select AAV vectors that we can use for shRNAs. We can also create the ideal vector for you through our custom vector service. Contact us with your requests at info@stratech.co.uk
SkyangBio uses a functional titer method for determining lentiviral titers. This is a more accurate calculation than physical titer methods.
SkyangBio’s shERWOOD-UltramiR™ shRNA libraries are next-generation, vector-based RNAi triggers designed using the proprietary shERWOOD algorithm that was developed and validated in Dr. Gregory Hannon’s laboratory at Cold Spring Harbor Laboratory (Knott et al 2014). The library uses an alternate microRNA scaffold called “UltramiR“. The UltramiR scaffold has been optimized for increased shRNA processing and potency based on the key determinants for primary microRNA processing (Auyeung et al 2013).
The shERWOOD algorithm is based on the functional testing of over 250,000 shRNA sequences using a high-throughput sensor assay and uses key sequence characteristics for predicting shRNA potency to select the rare shRNA designs that are potent at single copy representation in the genome.
Don’t see your question? Contact Us and we will be happy to help!
Yes, SkyangBio can quote to provide an extra copy of each 96-well plate (glycerol stock).
Yes. SkyangBio can provide a quote for preparing 96-well plates of purified plasmid DNA. Please contact us at info@stratech.co.uk
You will receive 1,089 96-well plates containing ~300ul of bacterial glycerol stock in each well. You will also receive the datafile showing shRNA construct is in each well.
Yes, send a list of your genes to info@stratech.co.uk and we will provide you with a quote and additional details.
Under general terms, clones from SkyangBio’s whole genome arrayed libraries can only be shared with lab members. If you would like to discuss alternative terms that allows further access to the library clones, contact us at info@stratech.co.uk
Yes, just request this information from info@stratech.co.uk and we can provide this to you.
SkyangBio’s shERWOOD-UltramiR™ shRNA libraries are next-generation, vector-based RNAi triggers designed using the proprietary shERWOOD algorithm that was developed and validated in Dr. Gregory Hannon’s laboratory at Cold Spring Harbor Laboratory (Knott et al 2014). The library uses an alternate microRNA scaffold called “UltramiR“. The UltramiR scaffold has been optimized for increased shRNA processing and potency based on the key determinants for primary microRNA processing (Auyeung et al 2013).
The shERWOOD algorithm is based on the functional testing of over 250,000 shRNA sequences using a high-throughput sensor assay and uses key sequence characteristics for predicting shRNA potency to select the rare shRNA designs that are potent at single copy representation in the genome.
Don’t see your question? Contact Us and we will be happy to help!
Yes, SkyangBio can quote to provide an extra copy of each 96-well plate (glycerol stock).
Yes. SkyangBio can provide a quote for preparing 96-well plates of purified plasmid DNA. Please contact us at info@stratech.co.uk
You will receive 1,089 96-well plates containing ~300ul of bacterial glycerol stock in each well. You will also receive the datafile showing shRNA construct is in each well.
Yes, send a list of your genes to info@stratech.co.uk and we will provide you with a quote and additional details.
Under general terms, clones from SkyangBio’s whole genome arrayed libraries can only be shared with lab members. If you would like to discuss alternative terms that allows further access to the library clones, contact us at info@stratech.co.uk
Yes, just request this information from info@stratech.co.uk and we can provide this to you.
Thousands of off-the-shelf cDNA and ORF clones and yeast deletion strains.
SkyangBio provides several valuable genomic resources to the scientific community. One of these resources is the Mammalian Gene Collection (MGC). The NIH chose SkyangBio as the official archive site for the collection, and as such, we offer these high-quality, full-length cDNA clones at a budget friendly price and with a rapid turnaround time. Use our gene search tool to search through the thousands of human and mouse cDNA clones to find your gene of interest. You will find a few other species represented in the collection as well. We also offer ORF clones in Gateway™ Entry Vectors and Lentiviral Vectors. Search the links below to explore other resources provided by SkyangBio. Are you interested in having SkyangBio make your clones available to the scientific community? Contact us at info@stratech.co.uk
The Mammalian Gene Collection (MGC) was created though an NIH initiative involving several labs to create the most comprehensive cDNA and ORF libraries available to the scientific community. All cDNA and ORF information can be researched through NCBI Genbank, and individual clones can be conveniently ordered through SkyangBio’s gene search tool.
Constructs are delivered as glycerol stocks
The MGC premeir cDNA and ORF collections are composed of various cloning vectors.
Don’t see your question? Contact Us and we will be happy to help!
Please contact us at info@stratech.co.uk and let us know what you are looking for. It is possible we can find your gene using an alias gene symbol or different gene identifier. If we don’t have the gene, we are happy to prepare a quote for a custom gene synthesis.
The MGC premier cDNA and ORF clones were created by an NIH initiative that involved a consortium of laboratories. All of the cDNA and ORF clones in the library were sequenced by the consortium to confirm that each clone is full-length. The data for all of the MGC cDNA and ORF clones can be found in GenBank. The SkyangBio gene search results conveniently link each clone to GenBank for your review.
SkyangBio is a distributor (and the official archive site) for the MGC libraries. When you purchase a clone from one of these libraries, we will prepare a fresh glycerol stock clone and will ship it overnight to your lab. Good laboratory practice is to sequence-confirm the clone before starting your experiment. SkyangBio offers the option to perform this for you. We will end-sequence the cDNA or ORF clone and will BLAST that sequence against the sequence for that clone in GenBank. The sequence-confirmed clone will be shipped to you along with the BLAST report showing the clone has been sequence-confirmed. Note that we do not sequence the entire insert. We sequence enough of the insert to show that you have the correct clone.
cDNA clones will contain the untranslated regions (UTRs), where the ORF (Open Reading Frame) will have the UTRs removed.
The ORF clones were made by a consortium of laboratories. Some labs chose to include a stop codon in the ORF. Other labs chose to remove the stop codon in the ORF to make it easier to add a tag or linker if needed.
You will receive a 2ml tube that contains 600ul bacterial glycerol stock.
SkyangBio uses different cap colors to indicate the bacterial antibiotic selection. Red caps need to be grown with ampicillin (or carbenicillin). Green caps need to be grown with kanamycin. Black caps need to be grown with chloramphenicol.
Yes, SkyangBio can cherry pick your genes of interest into 96-well plates. Contact us at info@stratech.co.uk, let us know what you need and we will prepare a quote for you.
Yes, SkyangBio offers bulk discounts on cDNA and ORF clones. Just contact us at info@stratech.co.uk and let us know what you need, and we will prepare a quote for you.
Yes, SkyangBio can provide you with a copy of the entire arrayed libraries. Just contact us at info@stratech.co.uk and let us know what you need, and we will prepare a quote for you.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at info@stratech.co.uk and let us know what you need, and we will prepare a quote for you.
Yes, SkyangBio offers clones on Whatman blotting paper. Just contact us at info@stratech.co.uk and let us and we can provide details and a quote.
Please contact us info@stratech.co.uk for more information.
Not all of the cDNA and ORF clones are in an expression vector. Some of the cDNA clones are in the pCMV-SPORT6 vector which is a mammalian expression vector. Some of the ORF clones are in the pLX304 vector which is a lentiviral expression vector.
MGC premier cDNA and ORF arrayed libraries are genome-scale collections developed through academic and commercial collaborations using the most rigorous sequencing process, ensuring the highest quality and confidence.
The goal of the Mammalian Gene Collection (MGC), a trans-NIH initiative, was to provide researchers with unrestricted access to sequence-validated, full-length, protein-coding (FL-CDS) cDNA clones. SkyangBio offers the complete MGC collection – separated into human and mouse arrayed libraries. Sub-libraries containing only pCMV-SPORT6 expression-ready clones are also conveniently available.
The MGC premier human ORFeome Collaboration Library currently represents 9,804 genes with 16,581 clones. The majority of the ORFeome collaboration targets were generated by the Dana Farber Cancer Institute-Center for Cancer Systems Biology (DFCI-CCSB). These full-length, annotated and sequence-verified ORFs originate from the existing collection of MGC premier full-length human cDNA clones and have been transferred into Gateway Entry vectors as a ready-to-use resource for recombinant protein expression. For additional convenience and versatility, the ORF clones are available in two formats, with and without stop codons. The ORF clones without stop codons facilitate the synthesis of either C- or N- terminal fusion proteins and clones with stop codons enable the synthesis of native proteins in addition to N-terminal fusion proteins.
The MGC premier ORFeome Collaboration Collection is also available for the mouse and represents 1,434 genes with 1,956 clones.
The MGC premier human ORFeome version 8.1 (hORFeome v8.1) is the newest version of the human ORFeome developed by the Center for Cancer Systems Biology (CCSB). This collection is derived from the Mammalian Gene Collection (MGC) and contains over 11,000 clonally-derived ORFs that represent 11,000 human genes. Each ORF clone in the collection has been verified by next generation sequencing and is provided in a Gateway adapted entry vector for fast and convenient transfer to any compatible expression vector.
This human Lentiviral ORF Expression Library was developed through the collaborative efforts of Dana-Farber Cancer Institute, The Broad Institute and the Center for Cancer Systems Biology (CCSB). The collection, which includes over 13,000 ORFs that represent approximately 11,000 genes, provides the most fully sequenced and annotated version of the human ORFeome available. For added convenience the lentiviral ORF expression vector was created to enable expression of a protein of interest with a V5 fusion tag for western blot detection, purification, co-immunoprecipitation, protein localization and FACS analysis.
Don’t see your question? Contact Us and we will be happy to help!
Please contact us at info@stratech.co.uk and let us know what you are looking for. It is possible we can find your gene using an alias gene symbol or different gene identifier. If we don’t have the gene, we are happy to prepare a quote for a custom gene synthesis.
Yes. SkyangBio can provide a quote for preparing 96-well plates of purified plasmid DNA.
Yes, SkyangBio can quote to provide an extra copy of each 96-well plate (glycerol stock).
Yes, just send a list of your genes to info@stratech.co.uk and we will provide you with a quote and additional details.
Yes, the data file for each library is available for download, please contact us at info@stratech.co.uk and we will provide you the data files
Order any yeast deletion mutant strain from the S. cerevisiae genome-wide yeast deletion collection. The collection includes over 20,000 deletion strains corresponding to 5,916 genes (including 1,159 essential genes). Each strain has a complete start-codon to stop-codon deletion of an open reading frame (ORF) that is flanked by two molecular barcodes.
Don’t see your question? Contact Us and we will be happy to help!
You will receive a 2ml tube that contains 600ul of glycerol stock for your yeast deletion strain.
SkyangBio grows yeast deletion strains in YPD media with G418 at 30C for 24-48 hours. After incubation, we add 20% glycerol, shake, and freeze at -80C.
The parental strain used was S288C (Haploid-Mating type alpha). The genotype was MATα SUC2 gal2 mal2 mel flo1 flo8-1 hap1 ho bio1 bio6.
Strain identity can be confirmed by PCR using primers specific to the gene and the selection marker. Knockout alleles require primers specific to the knockout cassette for PCR amplification while wild type alleles are PCR amplified by gene specific primers. Haploid and homozygous deletion strains are positive for knock out allele PCR products and negative for wild type allele PCR products. Heterozygous strains should be positive for all bands. Primer sequences and expected PCR amplicon sizes are available on the clone details page for each strain. More information is available in the Yeast Deletion Product Guide.
Each gene was knocked out using homologous recombination and replaced with a Kanamycin cassette and uptags/downtags/barcode so the strains can be selected on G418 and identified in high throughput screening.
Try these resources: 1.) Shoemaker, DD. et al. (1996). Quantitative phenotypic analysis of yeast deletion mutants using a highly parallel molecular bar-coding strategy. Nat Genet. 1996 Dec;14(4):450-6., 2.) https://www.nature.com/articles/ng1296-450
No. Skyang does not make custom yeast deletion strains. We only distribute the strains listed on the website.
In 1996 S. cerevisiae became the first eukaryotic genome to be fully-sequenced, enabling the creation of the first molecular-barcoded, genome-wide yeast deletion collection (1, 2). The collection includes over 20,000 deletion strains corresponding to 5,916 genes (including 1,159 essential genes).
Each strain has a complete start-codon to stop-codon deletion of an open reading frame (ORF) that is flanked by two molecular barcodes. This collection has been used successfully in a number of screens and has been cited over 780 times (3).
Pooled format is available for simultaneous analysis of large numbers of deletion strains through selective growth conditions. Identification and quantitation of the deletions surviving selection can be determined by hybridizing the deletions to a microarray containing all the barcode oligos. The barcode oligos are unique to each deletion, allowing rapid, high-throughput identification of the deletions. Each Yeast Deletion Pool contains all the deletions within the strain, equally represented within the pool. Yeast Deletion Pools are supplied in ten tubes, each containing 500 µl of the pooled deletions.
The Yeast Deletion Collections were created by systematically targeting every open reading frame (ORF) and creating a start- to stop-codon deletion. The cassette used to delete the ORF consists of a selectable marker (kanamycin-resistance KanMX4) flanked by two barcode sequences. These are referred to as the UPTAG and DOWNTAG and are unique to each gene. The 5′ and 3′ sequences of the cassette are homologous to the targeted gene to allow recombination. Following recombination, the selection marker replaces the ORF and the barcodes are integrated into the genome.
The UPTAG and DOWNTAG barcodes (Figure 1) allow high-throughput parallel screening. Starting with a population where each yeast deletion strain is represented equally, selective pressure can be applied. Strains resistant to the selection become more highly represented than sensitive strains during growth of the culture. The changes in population correlate with changes in the barcode representation and can be analyzed by microarray or next generation sequencing.
The selective pressure is determined by the type of screen. One of the initial validations of the yeast knockout collection highlights its utility and flexibility by performing a number of screens using selection for growth in high salinity, low salinity, high pH, changes in carbon source and peroxide stress1. There have been a number of screens since including, but not limited to, examining exposure to ionizing radiation, DNA damaging reagents and defects in cell division2.
Schematic of homologous recombination between the barcoded deletion cassette and the yeast chromosomal DNA. (Graphics adapted from publication: The uses of genome-wide yeast mutant collections: Bart Scherens and Andre Goffeau, Genome Biology 2004, 5:229.)
Figure 1: Schematic of barcode screening analysis showing
differential selection of yeast deletion strains and
readout on microarray. (Graphics adapted from publication:
The uses of genome-wide yeast mutant collections:
Bart Scherens and Andre Goffeau, Genome Biology 2004, 5:229.)
| Pooled Libary | Product Code |
| Yeast Detection Collection Essential Genes | TKY3504P |
| Yeast Detection Collection Homozygous Diploid | TKY3500P |
| Yeast Detection Collection Heterozygous Diploid | TKY3501P |
| Yeast Deletion Collection Haploid Mat-a | TKY3502P |
| Yeast Delection Collectin Haploid Mat-alpha | TKY3503P |
| Pooled Libary | Product Code |
| Yeast Detection Collection Essential Genes | TKY3504 |
| Yeast Detection Collection Homozygous Diploid | TKY3500 |
| Yeast Detection Collection Heterozygous Diploid | TKY3501 |
| Yeast Deletion Collection Haploid Mat-a | TKY3502 |
| Yeast Delection Collectin Haploid Mat-alpha | TKY3503 |
Don’t see your question? Contact Us and we will be happy to help!
For arrays, you will receive 96-well plates containing around 200ul of culture in YPD+G418+glycerol. Each plate is clearly labeled to match the datafile.
SkyangBio grows yeast deletion strains in YPD media with G418 at 30C for 24-48 hours. After incubation, we add 20% glycerol, shake, and freeze at -80C.
SkyangBio follows a common pooling process where we stamp all 96-well plates containing the deletion strains onto YPD agar+G418. We allow all deletion strains to grow to a uniform colony size and then we add YPD media to each agar plate and gently scrape the colonies into one flask. The pool of deletion strains is then mixed well and dispensed into individual vials. This is a standard protocol that has been used by the yeast community over the past several years. No further QC is applied to the pools.
SkyangBio works with academic labs across the globe to provide genomic tools that help researchers succeed with their research interests. Those tools can then be offered to the scientific community for continued discovery. Here we offer inducible lentiviral ORF clones prepared for the Kentsis lab at Memorial Sloan Kettering Cancer Center. These inducible ORFs are stem cell regulator candidates in acute myeloid leukemia.
| Format | Human Gene | Product Code |
| Glycerol Stock | BTG2 | TLO-AK-G-1001 |
| EGR1 | TLO-AK-G-1002 | |
| ERG | TLO-AK-G-1003 | |
| ETS1 | TLO-AK-G-1004 | |
| FLI1 | TLO-AK-G-1005 | |
| FOSB | TLO-AK-G-1006 | |
| FOSL2 | TLO-AK-G-1007 | |
| GATA2 | TLO-AK-G-1008 | |
| JUNB | TLO-AK-G-1009 | |
| JUND | TLO-AK-G-1010 | |
| KLF2 | TLO-AK-G-1011 | |
| KLF4 | TLO-AK-G-1012 | |
| KLF6 | TLO-AK-G-1013 | |
| LYL1 | TLO-AK-G-1014 | |
| MEF2C | TLO-AK-G-1015 | |
| MYB | TLO-AK-G-1016 | |
| RUNX1 | TLO-AK-G-1017 | |
| ZFP36L1 | TLO-AK-G-1018 | |
| Lentiviral Particles (100ul of>5×10^6 TU/ml) | BTG2 | TLO-AK-V-1001 |
| EGR1 | TLO-AK-V-1002 | |
| ERG | TLO-AK-V-1003 | |
| ETS1 | TLO-AK-V-1004 | |
| FLI1 | TLO-AK-V-1005 | |
| FOSB | TLO-AK-V-1006 | |
| FOSL2 | TLO-AK-V-1007 | |
| GATA2 | TLO-AK-V-1008 | |
| JUNB | TLO-AK-V-1009 | |
| JUND | TLO-AK-V-1010 | |
| KLF2 | TLO-AK-V-1011 | |
| KLF4 | TLO-AK-V-1012 | |
| KLF6 | TLO-AK-V-1013 | |
| LYL1 | TLO-AK-V-1014 | |
| MEF2C | TLO-AK-V-1015 | |
| MYB | TLO-AK-V-1016 | |
| RUNX1 | TLO-AK-V-1017 | |
| ZFP36L1 | TLO-AK-V-1018 |
| Format | Product Code |
| Lentiviral particles (100ul of >5×10^6 TU/ml) | TLO-AK-V-Pool |
Negative control vectors are a required component of your experimental design to help understand the potential non-specific effects associated with lentiviral transduction in gene expression experiments.
SkyangBio’s empty vector negative controls will include all the elements that are contained in the active LentiORF vectors with the exception that the MCS (multiple cloning site) is intact with no additional DNA inserted. Empty vector negative controls undergo the same rigorous QC used for all LentiORF vectors so that you can be confident in the high-quality reagents you are using in your gene expression experiments.
Constructs are delivered as glycerol stocks or ready-to-use lentiviral particles.
| Format | Selection Marker | Flurescent Reporter | Product Code |
| Glycerol Stock | Blasticidin | eGFP | |
| Blasticidin | mCherry | TLO2043.C | |
| Hygromycin | eGFP | TLO2003.C | |
| Hygromycin | mCherry | TLO2044.C | |
| Neomycin | eGFP | Not Available | |
| Neomycin | mCherry | TLO2045.C | |
| Puromycin | eGFP | TLO2005.C | |
| Puromycin | mCherry | TLO2037.C | |
| Lentiviral Particles (100ul of>5×10^6 TU/ml) | Blasticidin | eGFP | TLOV2002.C |
| Blasticidin | mCherry | ||
| Hygromycin | eGFP | TLOV2003.C | |
| Hygromycin | mCherry | ||
| Neomycin | eGFP | Not Available | |
| Neomycin | mCherry | ||
| Puromycin | eGFP | ||
| Puromycin | mCherry | TLOV2037.C |
| Format | Marker/Reporter | Product Code |
| Glycerol Stock | Blasticidin | |
| Hygromycin | TLO1002.C | |
| Neomycin | TLO1017.C | |
| Puromycin | TLO1004.C | |
| eGFP | TLO2001.C | |
| mCherry | TLO2046.C | |
| Lentiviral Particles (100ul of>5×10^6 TU/ml) | Blasticidin | TLOV1001.C |
| Hygromycin | TLOV1002.C | |
| Neomycin | ||
| Puromycin | TLOV1004.C | |
| eGFP | TLOV2001.C | |
| mCherry | TLOV2046.C |
| Format | Selection Marker | Flurescent Reporter | Product Code |
| Glycerol Stock | Blasticidin | eGFP | TLO2012.C |
| Blasticidin | mCherry | ||
| Hygromycin | eGFP | TLO2013.C | |
| Hygromycin | mCherry | TLO2041.C | |
| Neomycin | eGFP | Not Available | |
| Neomycin | mCherry | TLO2042.C | |
| Puromycin | eGFP | TLO2015.C | |
| Puromycin | mCherry | TLO2039.C | |
| Lentiviral Particles (100ul of>5×10^6 TU/ml) | Blasticidin | eGFP | TLOV2012.C |
| Blasticidin | mCherry | TLOV2040.C | |
| Hygromycin | eGFP | TLOV2013.C | |
| Hygromycin | mCherry | TLOV2041.C | |
| Neomycin | eGFP | Not Available | |
| Neomycin | mCherry | TLOV2042.C | |
| Puromycin | eGFP | TLOV2015.C | |
| Puromycin | mCherry | TLOV2039.C |
| Format | Marker/Reporter | Product Code |
| Glycerol Stock | Blasticidin | TLO1005.C |
| Hygromycin | TLO1006.C | |
| Neomycin | TLO1018.C | |
| Puromycin | TLO1008.C | |
| eGFP | TLO2011.C | |
| mCherry | TLO2047.C | |
| Lentiviral Particles (100ul of>5×10^6 TU/ml) | Blasticidin | TLOV1005.C |
| Hygromycin | TLOV1006.C | |
| Neomycin | TLOV1018.C | |
| Puromycin | TLOV1008.C | |
| eGFP | TLOV2011.C | |
| mCherry | TLOV2047.C |
Don’t see your question? Contact Us and we will be happy to help!
You will receive one 2ml tube. The tube will contain 600ul of bacterial glycerol stock.
You will receive 100ul of viral particles for the negative control. The 100ul will be split into 4 tubes of 25ul.
For constitutive vectors, you will receive 100ul of 1×10^8 TU/ml. For inducible vectors, you will receive 1×10^7 TU/ml.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at sales@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio is very flexible in what we can offer. Just contact us at sales@stratech.co.uk and let us know what you need, and we will send you a quote.
Yes, SkyangBio offers clones on Whatman blotting paper. Just contact us at sales@stratech.co.uk and let us know what you need, and we can provide details and a quote.
SkyangBio offers a large selection of lentiviral cloning vectors for CRISPR, shRNA and Gene Expression applications. A menu of promoters, selectable markers and fluorescent reporters allows you to customize your vector for your specific application.
All SkyangBio lentiviral vectors are 3rd generation transfer plasmids and have been optimized for efficient 3rd generation lentiviral packaging. The use of a 3rd generation packaging system significantly reduces the likelihood of generating replication-competent lentivirus and thus results in improved safety over earlier packaging systems. This is accomplished by introducing several key changes:
Note that a 3rd generation transfer plasmid can be used with a 2nd generation packaging system, but a 2nd generation transfer plasmid cannot be used with a 3rd generation packaging system.
Cloning vectors are typically shipped the day of order.
Biosafety should always be considered, and your biosafety office can provide more information on your institution’s best practices with regard to lentiviral research.
NOTE: Our cloning vectors are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our cloning vectors may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
Our CRISPR-Cas9 cloning vectors are ideally suited for cloning gRNAs by following the simple cloning steps outlined in the cloning guidelines (see below). A menu of promoters, selectable markers and fluorescent reporters allows you to customize your vector for your specific application.
Advantages:
All SkyangBio lentiviral vectors are 3rd generation transfer plasmids and have been optimized for efficient 3rd generation lentiviral packaging. The use of a 3rd generation packaging system significantly reduces the likelihood of generating replication-competent lentivirus and thus results in improved safety over earlier packaging systems. This is accomplished by introducing several key changes:
Dependence of Tat transactivation is eliminated using a chimeric 5′ LTR fused to a heterologous promoter on the transfer plasmid.
4 plasmids are required for generating virus because the packaging system is split into two plasmids to separate Rev from Gag and Pol
All expression vectors have been made self-inactivating (SIN) via a deletion in 3′ LTR (ΔU3). This deletion is transferred into the 5’LTR after one round of reverse transcription and abolishes transcription of the full-length virus after it has incorporated into a host cell.
Constructs are delivered as glycerol stocks.
| Promoter | Selection Marker | Fluorescent Reporter | Product Code |
| EFS | NONE | NONE | Not available |
| ZsGreen | TCV1004 | ||
| mCherry | TCV1023 | ||
| Blasticidin | NONE | TCV1001 | |
| ZsGreen | TCV1013 | ||
| mCherry | TCV1025 | ||
| Hygromycin | NONE | TCV1002 | |
| ZsGreen | TCV1014 (V429) | ||
| mCherry | TCV1026 (V428) | ||
| Puromycin | NONE | TCV1003 | |
| ZsGreen | TCV1015 | ||
| mCherry | TCV1027 | ||
| hCMV | NONE | NONE | Not available |
| ZsGreen | TCV1008 | ||
| mCherry | TCV1024 (V412) | ||
| Blasticidin | NONE | TCV1005 (V074) | |
| ZsGreen | TCV1016 (V431) | ||
| mCherry | TCV1028 (V433) | ||
| Hygromycin | NONE | TCV1006 (V393) | |
| ZsGreen | TCV1017 (V435) | ||
| mCherry | TCV1029 (V434) | ||
| Puromycin | NONE | TCV1007 (V075) | |
| ZsGreen | TCV1018 (V430) | ||
| mCherry | TCV1030 (V432) | ||
| SFFV | NONE | NONE | Not available |
| ZsGreen | TCV1012 | ||
| mCherry | TCV1025 | ||
| Blasticidin | NONE | TCV1009 | |
| ZsGreen | TCV1019 | ||
| mCherry | TCV1032 | ||
| Hygromycin | NONE | TCV1010 | |
| ZsGreen | TCV1020 | ||
| mCherry | TCV1033 | ||
| Puromycin | NONE | TCV1011 | |
| ZsGreen | TCV1021 | ||
| mCherry | TCV1034 |
| Promoter | Selection Marker | Fluorescent Reporter | Product Code |
| EFS | None | Ametrine | TCV1022 |
Don’t see your question? Contact Us and we will be happy to help!
You will receive a 2ml tube containing 600ul of glycerol stock of the cloning vector. Follow the product guidelines to ensure you store and handle your cloning vector properly.
Please refer to the cloning guidelines document on our website. If you still have questions, please contact us at info@stratech.co.uk.
Yes. SkyangBio has a vector construction service where we can modify any of our vector backbones to contain the elements needed for your research. We also have many other types of vectors that we can use for a backbone (AAV, mammalian expression, retroviral).
Yes, a 3rd generation transfer plasmid can be used with a 2nd generation packaging system. However, a 2nd generation transfer plasmid cannot be used with a 3rd generation packaging system. Biosafety should always be considered, and your biosafety office can provide more information on your institution’s best practices with regard to lentiviral research.
Yes, your clones may be shared with academic colleagues. However, empty cloning vectors may not be transferred to any third party.
Yes, your clones may be shared with academic colleagues through Addgene after receiving written consent from SkyangBio. Please ensure SkyangBio is listed as the provider of the cloning vector on the Addgene purchasing page. Contact info@stratech.co.uk for written consent or further information.
SkyangBio cloning vectors may not be used or modified for creating commercial products or to offer commercial services without written consent from SkyangBio. Please contact us for further information at info@stratech.co.uk.
Our shRNA cloning vectors are ideally suited for cloning shRNAs by following the simple cloning steps outlined in the cloning guidelines (see below).
All SkyangBio lentiviral vectors are 3rd generation transfer plasmids and have been optimized for efficient 3rd generation lentiviral packaging. The use of a 3rd generation packaging system significantly reduces the likelihood of generating replication-competent lentivirus and thus results in improved safety over earlier packaging systems. This is accomplished by introducing several key changes:
Constructs are delivered as glycerol stocks.
NOTE: Our cloning vectors are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our cloning vectors may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
| Reporter | Marker | Product Code |
| None | Blasticidin | TCV2101 (V208) |
| Hygromycin | TCV2102 | |
| Puromycin | TCV2103 (V207) | |
| ZsGreen | Blasticidin | TCV2104 (V152) |
| Hygromycin | TCV2105 (V451) | |
| Puromycin | TCV2106 (V132) | |
| mCherry | Blasticidin | TCV2108 (V418) |
| Hygromycin | TCV2109 (V450) | |
| Puromycin | TCV2107 (V420) |
| Reporter | Marker | Product Code |
| None | Blasticidin | TCV2201 (V210) |
| Hygromycin | TCV2202 | |
| Puromycin | TCV2203 (V168) | |
| ZsGreen | Blasticidin | TCV2204 (V137) |
| Hygromycin | TCV2205 (V136) | |
| Puromycin | TCV2206 (V028) | |
| mCherry | Blasticidin | TCV2208 (V415) |
| Hygromycin | TCV2209 (V452) | |
| Puromycin | TCV2207 (V407) |
| Reporter | Marker | Product Code |
| None | Blasticidin | TCV2301 (V212) |
| Hygromycin | TCV2302 (V347) | |
| Puromycin | TCV2303 (V213) | |
| ZsGreen | Blasticidin | TCV2304 (V241) |
| Hygromycin | TCV2305 (V454) | |
| Puromycin | TCV2306 (V133) | |
| mCherry | Blasticidin | TCV2308 (V417) |
| Hygromycin | TCV2309 (V453) | |
| Puromycin | TCV2307 (V403) |
| Reporter | Marker | Product Code |
| None | Blasticidin | TCV2401 (V215) |
| Hygromycin | TCV2402 | |
| Puromycin | TCV2403 (V216) | |
| ZsGreen | Blasticidin | TCV2404 (V218) |
| Hygromycin | TCV2405 (V127) | |
| Puromycin | TCV2406 (V021) | |
| mCherry | Blasticidin | TCV2408 (V416) |
| Hygromycin | TCV2409 (V455) | |
| Puromycin | TCV2407 (V419) |
| Vector | Product Code |
| pLMN-ZsGreen-Neomycin | TCV2501 (V015) |
| pLMPd-Ametrine | TCV2502 (V020) |
Don’t see your question? Contact Us and we will be happy to help!
You will receive a 2ml tube containing 600ul of glycerol stock of the cloning vector. Follow the product guidelines to ensure you store and handle your cloning vector properly.
Please refer to the cloning guidelines document on our website. If you still have questions, please contact us at info@stratech.co.uk.
Yes. SkyangBio has a vector construction service where we can modify any of our vector backbones to contain the elements needed for your research. We also have many other types of vectors that we can use for a backbone (AAV, mammalian expression, retroviral).
Yes, a 3rd generation transfer plasmid can be used with a 2nd generation packaging system. However, a 2nd generation transfer plasmid cannot be used with a 3rd generation packaging system. Biosafety should always be considered, and your biosafety office can provide more information on your institution’s best practices with regard to lentiviral research.
Yes, your clones may be shared with academic colleagues. However, empty cloning vectors may not be transferred to any third party.
Yes, your clones may be shared with academic colleagues through Addgene after receiving written consent from SkyangBio. Please ensure SkyangBio is listed as the provider of the cloning vector on the Addgene purchasing page. Contact info@stratech.co.uk for written consent or further information.
SkyangBio cloning vectors may not be used or modified for creating commercial products or to offer commercial services without written consent from SkyangBio. Please contact us for further information at info@stratech.co.uk.
Our overexpression cloning vectors are ideally suited for cloning your gene of interest by following the simple cloning steps outlined in the cloning guidelines (see below).
All SkyangBio lentiviral vectors are 3rd generation transfer plasmids and have been optimized for efficient 3rd generation lentiviral packaging. The use of a 3rd generation packaging system significantly reduces the likelihood of generating replication-competent lentivirus and thus results in improved safety over earlier packaging systems. This is accomplished by introducing several key changes:
Constructs are delivered as glycerol stocks.
NOTE: Our cloning vectors are to be used for Research Use Only. They may not be used for any other purpose, including, but not limited to, use in humans, therapeutic or diagnostic use, or commercial use of any kind. Our cloning vectors may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products or to provide a service to third parties without our prior written approval.
| Marker/Reporter | Product Code |
| Blasticidin | TLO1001.CV |
| Hygromycin | TLO1002.CV |
| Neomycin | TLO1017.CV |
| Puromycin | TLO1004.CV |
| eGFP | TLO2001.CV |
| mCherry | TLO2046.CV |
| Selection Marker | Fluorescent Marker | Product Code |
| Blasticidin | eGFP | TLO2002.CV |
| mCherry | TLO2043.CV | |
| Puomycin | eGFP | TLO2005.CV |
| mCherry | TLO2037.CV | |
| Hygromycin | eGFP | TLO2003.CV |
| mCherry | TLO2044.CV | |
| Neomycin | eGFP | Not Available |
| mCherry | TLO2045.CV |
| Marker/Reporter | Product Code |
| Blasticidin | TLO1005.CV |
| Hygromycin | TLO1006.CV |
| Neomycin | TLO1018.CV |
| Puromycin | TLO1008.CV |
| eGFP | TLO2011.CV |
| mCherry | TLO2047.CV |
| Selection Marker | Fluorescent Marker | Product Code |
| Blasticidin | eGFP | TLO2012.CV |
| mCherry | TLO2040.CV | |
| Puomycin | eGFP | TLO2015.CV |
| mCherry | TLO2039.CV | |
| Hygromycin | eGFP | TLO2013.CV |
| mCherry | TLO2041.CV | |
| Neomycin | eGFP | Not Available |
| mCherry | TLO2042.CV |
Don’t see your question? Contact Us and we will be happy to help!
You will receive a 2ml tube containing 600ul of glycerol stock of the cloning vector. Follow the product guidelines to ensure you store and handle your cloning vector properly.
Please refer to the cloning guidelines document on our website. If you still have questions, please contact us at info@stratech.co.uk.
Yes. SkyangBio has a vector construction service where we can modify any of our vector backbones to contain the elements needed for your research. We also have many other types of vectors that we can use for a backbone (AAV, mammalian expression, retroviral).
Yes, a 3rd generation transfer plasmid can be used with a 2nd generation packaging system. However, a 2nd generation transfer plasmid cannot be used with a 3rd generation packaging system. Biosafety should always be considered, and your biosafety office can provide more information on your institution’s best practices with regard to lentiviral research.
Yes, your clones may be shared with academic colleagues. However, empty cloning vectors may not be transferred to any third party.
Yes, your clones may be shared with academic colleagues through Addgene after receiving written consent from SkyangBio . Please ensure SkyangBio is listed as the provider of the cloning vector on the Addgene purchasing page. Contact info@stratech.co.uk for written consent or further information.
SkyangBio cloning vectors may not be used or modified for creating commercial products or to offer commercial services without written consent from SkyangBio. Please contact us for further information at info@stratech.co.uk.
