DHEA-S EIA Kit Research

Catalogue Number: 1-1252-SAL

Manufacturer:	Salimetrics LLC
Type:	Human ELISA Kit
Shipping Condition:	Blue Ice
Storage Condition:	4°C
Unit(s):	96 Tests
Range:	188.9 pg/mL- 15,300 pg/mL
Sensitivity:	43 pg/mL
Sample type:	Saliva
Sample size:	100 µL
Application:	ELISA

Overview
Datasheets

Description

Description: Salimetrics salivary assay kits are expertly designed, developed and validated to ensure accuracy in saliva and proven to deliver precision results for saliva biomarkers.The Salimetrics Salivary DHEA-S (DHEA-Sulfate) Enzyme Immunoassay Kit was specifically designed to standardize the detection of DHEA-S in saliva samples for research and biomedical laboratories. Using a small sample volume, this assay kit has an extended range which spans the expected DHEA-S levels found in human saliva. The average inter- and intra-assay precision coefficients of variation are low with no deleterious matrix effects often found in saliva which are characterized through dilution- and spike-recovery validation procedures. This DHEA-S assay kit has also been formatted to minimize cross reactivity for related steroids. DHEA-S is the most abundant steroid hormone in humans, with circulating concentrations approximately 250 and 500 times higher than those of its unsulfated analog, DHEA, in women and men, respectively. DHEA-S is not bound by sex hormone binding globulin (SHBG) in the blood stream and is readily available for conversion to other compounds. Unlike DHEA, DHEA-S does not normally exhibit any diurnal pattern of secretion and DHEA-S concentrations in saliva decrease markedly as flow rates increase. Levels of DHEA-S peak around the age of 20 to 30, and then decline to only 20-30% of peak levels by the age of 70 to 80. Critical illness and emotional or physical stress can also cause DHEA-S levels to decline. DHEA-S and DHEA are also synthesized directly by the central nervous system, where they appear to help protect nervous tissue against harmful agents. DHEA-S is not lipid soluble, and it cannot enter saliva by passive diffusion through cell membranes like most of the other steroid hormones. Instead, it enters saliva only by squeezing through the tight junctions between cells in the saliva glands, and it is too large to do this readily. It is therefore present in relatively small amounts. Binding proteins or enzymes in saliva that would affect the measurement of free DHEA-S appear largely to be absent.

1-1252 Protocol

Price

£314.00

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