Monoclonal Antibody to GAPDH (Clone: ABM22C5)
Catalogue Number: 10-10011-ABO
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Figure-2 : Immunohistochemical analysis of GAPDH in human Liver tissue using GAPDH antibody (Clone: ABM22C5) at 3 µg/ml.
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Figure-3: Immunohistochemical analysis of GAPDH in human Small intestine tissue using GAPDH antibody (Clone: ABM22C5) at 10 µg/ml.
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Figure-4: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 4 µg/ml on (1) CHO-K1 and (2) CHO/PD1 transfected Lysates.
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Figure-5: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 4 µg/ml on Raw cell Lysate.
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Figure-6: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 1 µg/ml on (1) h Lung, (2) h Testis, (3) h Liver and (4) h Ovary lysates.
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Figure-7: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 1 µg/ml on (1) h Brain, (2) m Brain, (3) h Pancrease, (4) h Kidney and (5) m Skeletal Muscle lysates.
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Figure-8: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 1 µg/ml on (1) PC3, (2) A549, (3)THP1, (4) Ramos, (5) HCT-116, (6) Raji and (7) MOLT-4 lysates.
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Figure-9: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 1 µg/ml on (1) U937, (2) Saos2, (3) A431, (4) K562, (5) U87, (6) MCF-7, (7) Snu1 and (8) Jurkat lysates.
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Figure-1: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 0.5 µg/ml on (1) Panc-1, (2) EL-4, (3) Jurkat and (4) HepG2 lysates.
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | GAPDH||GAPD||CDABP0047||OK/SW-cl.12 |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ug, 25 ug |
| Host name: | Mouse |
| Clone: | ABM22C5 |
| Isotype: | IgG1, kappa |
| Immunogen: | A partial length recombinant GAPDH protein (amino acids 120-320) was used as the immunogen for this antibody. |
| Application: | WB, IHC |
Description
Description: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) is an enzyme best known for its role in glycolysis. However, extra-glycolytic functions of GAPDH have been described, including regulation of protein expression via RNA binding. GAPDH binds to numerous AREs (adenine-uridine rich elements) from various mRNA 3'-untranslated regions in vitro and in vivo despite its lack of a canonical RNA binding motif. GAPDH specifically catalyzes the simultaneous phosphorylation and oxidation of glyceraldehyde 3-phosphate using NAD+ (Nicotinamide Adenine Dinucleotide) as a cofactor to produce glycerate 1,3-biphosphate and NADH. In addition to its role in energy production, GAPDH has been implicated in many cellular processes including DNA repair tRNA export, membrane fusion and transport, endocytosis and nuclear membrane assembly, and cell death.
Additional Text
Gene Name
GAPDH
Purification
Protein G purified
Uniprot ID
P04406
Gene ID
2597
Antibody Clonality
Monoclonal
Application Notes
Western blot analysis: 0.5-2 µg/ml, Immunohistochemical analysis: 5 µg/ml
Storage Note
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
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