Monoclonal Antibody to GAPDH (Clone: ABM22C5)

Catalogue Number: 10-10011-ABO

Manufacturer:Abeomics
Type:Monoclonal Primary Antibody - Unconjugated
Alias:GAPDH||GAPD||CDABP0047||OK/SW-cl.12
Shipping Condition:Blue Ice
Storage Condition:2-8°C
Unit(s): 100 ug, 25 ug
Host name: Mouse
Clone: ABM22C5
Isotype: IgG1, kappa
Immunogen: A partial length recombinant GAPDH protein (amino acids 120-320) was used as the immunogen for this antibody.
Application: WB, IHC

Description

Description: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) is an enzyme best known for its role in glycolysis. However, extra-glycolytic functions of GAPDH have been described, including regulation of protein expression via RNA binding. GAPDH binds to numerous AREs (adenine-uridine rich elements) from various mRNA 3'-untranslated regions in vitro and in vivo despite its lack of a canonical RNA binding motif. GAPDH specifically catalyzes the simultaneous phosphorylation and oxidation of glyceraldehyde 3-phosphate using NAD+ (Nicotinamide Adenine Dinucleotide) as a cofactor to produce glycerate 1,3-biphosphate and NADH. In addition to its role in energy production, GAPDH has been implicated in many cellular processes including DNA repair tRNA export, membrane fusion and transport, endocytosis and nuclear membrane assembly, and cell death.

Additional Text

Gene Name

GAPDH

Purification

Protein G purified

Uniprot ID

P04406

Gene ID

2597

Antibody Clonality

Monoclonal

Application Notes

Western blot analysis: 0.5-2 µg/ml, Immunohistochemical analysis: 5 µg/ml

Storage Note

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.