Monoclonal Antibody to Ebola GP II (Clone: ABM4C78)
Catalogue Number: 10-10021-ABO
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | GP |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ug, 25 ug |
| Host name: | Mouse |
| Clone: | ABM4C78 |
| Isotype: | IgG2a, lambda |
| Immunogen: | A partial Length recombinant GP II protein (N terminus 155aa) of Sudan Ebola virus was used as an immunogen for this antibody. |
| Application: | WB |
Description
Description: The Sudan ebola virus (SUDV) glycoprotein (GP) is an envelope glycoprotein that is present on the virion surface and is involved in receptor binding and mediating viral entry. It is composed of a trimer of heterodimers (GP1/GP2), where GP1 and GP2 remain covalently linked by a disulfide bond9, and the resulting GP1-GP2 pair trimerizes to form a ~450 kDa envelope spike on the viral surface. GP is synthesized as a single polypeptide of 676 amino acids in length that is post-translationally cleaved by furin to yield two subunits, GP1 and GP2. The GP2 subunit mediates virus-cell membrane fusion and viral entry. Structural analysis of the core domain of Ebola virus GP2 indicates that the fusion-active conformation of GP2 is similar to that of the other class I membrane fusion proteins, suggesting a common membrane fusion mechanism. In the endosome, GP2 releases from GP1 and undergoes irreversible conformational changes that drive fusion with host endosomal membranes. GP2 contains an N-terminal peptide, a hairpin-forming fusion loop, and two heptad repeats connected by a functionally important linker. The first heptad repeat of GP2 is wound around the base of GP1 in a metastable, prefusion-specific conformation.
Additional Text
Gene Name
GP
Purification
Protein G purified
Gene ID
3160774
Uniprot ID
Q7T9D9
Antibody Clonality
Monoclonal
Application Notes
Western blot analysis: 0.5-1 µg/ml
Storage Note
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
10-10021 Datasheet
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