Monoclonal Antibody to SARS-CoV-2 Nucleocapsid (Clone: ABM1F11.1E1)
Catalogue Number: 10-10032-ABO
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10-10032 Image
10-10032 Image
10-10032 Image
Figure-1: An indirect ELISA is carried out by coating nucleocapsid protein in serial dilution from 20 ng to 0.312 ng and using 100 ng of purified monoclonal antibodies 1F11.1E1. Peroxidase conjugated Goat-Anti mouse antibody was used at 1:5000 dilution.
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Figure-3: Western Blot analysis of SARS-CoV-2 Nucleocapsid Antibody: Anti- SARS-CoV-2 Nucleocapsid Antibody (Clone: ABM1F11.1E1) was used at 2 µg/ml on (1) SARS-CoV-2 virus infected Vero Cell lysate and (2) Mock infected lysate.
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Figure-2: Western Blot analysis: The purified antibodies 1F11.1E1 was tested on Nucleocapsid Recombinant protein at different concentrations, 0.1 (lane 1), 0.5 (lane 2), and 1.0µg/ml (lane 3), (4) RBD protein, (5)unrelated protein 1, (6) unrelated protein 2, (7) unrelated protein 3, to detect the specific binding. 25 ng of proteins was loaded per lane.
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 25 ug |
| Host name: | Mouse |
| Clone: | ABM1F11.1E1 |
| Isotype: | IgG1, kappa |
| Immunogen: | Full length recombinant SARS-CoV-2 nucleocapsid Protein was used as the immunogen for this antibody. |
| Application: | ELISA, WB |
Description
Description: The structural nucleocapsid (N) protein of nCoV/SARS-CoV-2/COVID-19 is a predicted 46 kDa phosphoprotein having 419 amino acid residues. A short Serine rich stretch and a recognized nuclear localization signal are the unique features of the nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19, which seems to have a little homology with the proteins of other members of this large corona virus family. These features also suggest the involvement of nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 in different stages of viral lifecycle. The protein has multifaceted activities including packing of the nCoV/SARS-CoV-2/COVID-19 viral genome into a helical ribonucleocapsid (RNP) and playing an important role in viral self-assembly causing nCoV/SARS-CoV-2/COVID-19. The nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 also forms dimer in the cell by self-association with the help of interactive C terminal domain.
Additional Text
Gene Name
N
Purification
Protein G purified
Antibody Clonality
Monoclonal
Application Notes
Recommended dilutions: WB: 0.1-1 µg/ml, ELISA: 1 µg/ml. However, this need to be optimized based on the research applications.
Gene ID
43740575
Uniprot ID
P0DTC9
Storage Note
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
10-10032 Datasheet
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