Monoclonal Antibody to SARS-CoV-2 nucleocapsid (Clone: ABM4H11.1C12)
Catalogue Number: 10-10035-ABO
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Figure-1: The Sandwich ELISA was carried out by coating the 96 well plate with 200 ng/well of ABM4H11.1C12 (Cat#10-10035) monoclonal antibody. The nucleocapsid protein (21-1003) was serially diluted from 10 ng to 0.0004ng in triplicates across rows. The 11-2004 polyclonal antibody (200 ng/well ) was used as detection antibody. Goat Anti-rabbit HRP was used as secondary antibody.
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Figure-2: Anti-nucleocapsid monoclonal Antibodies (4H11.1C12) were screened against different proteins to evaluate the specificity of the antibodies.
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Figure-4: An indirect ELISA is carried out by coating nucleocapsid protein in serial dilution from 20 ng to 0.312 ng and using 100 ng of purified monoclonal antibodies 4H11.1C12. Peroxidase conjugated Goat-Anti mouse antibody was used at 1:5000 dilution.
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Figure-5: Western Blot analysis of SARS-CoV-2 Nucleocapsid Antibody: Anti- SARS-CoV-2 Nucleocapsid Antibody (Clone: ABM4H11.1C12) was used at 4 µg/ml on (1) SARS-CoV-2 virus infected Vero Cell lysates and (2) Mock infected lysates.
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Figure-3: Western Blot analysis: The purified antibodies 4H11.1C12 was tested on Nucleocapsid Recombinant protein at different concentrations, 1 (lane 1), 0.5 (lane 2), and 0.1µg/ml (lane 3), (4) RBD protein, (5) unrelated protein 1, (6) unrelated protein 2, (7) unrelated protein 3, to detect the specific binding. 25 ng of proteins was loaded per lane.
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 25 ug |
| Host name: | Mouse |
| Clone: | ABM4H11.1C12 |
| Isotype: | IgG2b, kappa |
| Immunogen: | Full length recombinant SARS-CoV-2 nucleocapsid Protein was used as the immunogen for this antibody. |
| Application: | ELISA, WB |
Description
Description: The structural nucleocapsid (N) protein of nCoV/SARS-CoV-2/COVID-19 is a predicted 46 kDa phosphoprotein having 419 amino acid residues. A short Serine rich stretch and a recognized nuclear localization signal are the unique features of the nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19, which seems to have a little homology with the proteins of other members of this large corona virus family. These features also suggest the involvement of nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 in different stages of viral lifecycle. The protein has multifaceted activities including packing of the nCoV/SARS-CoV-2/COVID-19 viral genome into a helical ribonucleocapsid (RNP) and playing an important role in viral self-assembly causing nCoV/SARS-CoV-2/COVID-19. The nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 also forms dimer in the cell by self-association with the help of interactive C terminal domain.
Additional Text
Gene Name
N
Purification
Protein G purified
Antibody Clonality
Monoclonal
Application Notes
Recommended dilutions: WB: 0.1-1 µg/ml, ELISA: 1 µg/ml. However, this need to be optimized based on the research applications.
Gene ID
43740575
Uniprot ID
P0DTC9
Storage Note
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
10-10035 Datasheet
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