Monoclonal Antibody to Dnmt1 (Clone: ABM13B2)

Catalogue Number: 10-7002-ABO

Manufacturer:Abeomics
Type:Monoclonal Primary Antibody - Unconjugated
Alias:DNMT1||AIM||CXXC9||DNMT
Shipping Condition:Blue Ice
Storage Condition:2-8°C
Unit(s): 100 ug, 25 ug
Host name: Mouse
Clone: ABM13B2
Isotype: IgG1, kappa
Immunogen: A partial length recombinant DNMT1 protein (amino acids 128-429) was used as the immunogen for this antibody.
Application: FACS, WB, IHC

Description

Description: Dnmt1(DNA (cytosine-5-)-methyltransferase 1 ) is one of the most abundant DNA methyltransferase in mammalian tissues, where it associates with the replication machinery and restores symmetrical methylation at hemimethylated CpG sites generated by the semi-conservative DNA replication process. Dnmt1 comprises a regulatory N-terminal region and a C-terminal catalytic domain connected by a linker of seven glycine-lysine repeats. The N-terminal part contains a PCNA binding domain (PBD), a heterochromatin targeting sequence (TS), a CXXC-type zinc finger domain and two Bromo-Adjacent Homology domains (BAH1 and BAH2). The C-terminal domains of mammalian Dnmts contain all ten catalytic motifs identified in bacterial DNA (cytosine-5) methyltransferases. Dnmt1 maintains methylation patterns with high fidelity and is essential for embryonic development and genome integrity. The molecules interacting with Dnmt1, including RNA polymerase II, some RNA-binding proteins, and some specific Dnmt1-inhibitory RNA molecules are involved in chromatin organization, DNA repair, cell cycle regulation, and apoptosis.

Additional Text

Gene Name

DNMT1

Purification

Protein G purified

Uniprot ID

P26358

Gene ID

1786

Antibody Clonality

Monoclonal

Application Notes

Western blot analysis: 2-4 µg/ml, Immunohistochemical analysis: 5 µg/ml, FACS analysis: 0.5 µg/10^6 cells

Storage Note

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.