Monoclonal Antibody to MBD2/MBD3 (Clone: ABM14A8)

Catalogue Number: 10-7006-ABO

Manufacturer:Abeomics
Type:Monoclonal Primary Antibody - Unconjugated
Alias:MBD3
Shipping Condition:Blue Ice
Storage Condition:2-8°C
Unit(s): 100 ug, 25 ug
Host name: Mouse
Clone: ABM14A8
Isotype: IgG1, kappa
Immunogen: A partial length recombinant MBD3 protein (amino acids 1-290) was used as the immunogen for this antibody.
Application: FACS, WB, IHC

Description

Description: MBD2 is a subunit of the NuRD complex that is postulated to mediate gene repression via recruitment of the complex to methylated DNA. This protein binds around 1 kb downstream of the transcription start site of a subset of 400 CpG island promoters that are characterized by the presence of active histone marks, RNA polymerase II (Pol2) and low to medium gene expression levels and H3K36me3 deposition. Methylated MBD2 is involved in silencing methylated tumor suppressor genes as well as activation of pro-metastatic genes. MBD2 also plays a key role in regulating expression of a range of genes that are associated with optimal DC (Dendritic cells) activation and function.

Additional Text

Gene ID

53615

Gene Name

MBD3

Purification

Protein G purified

Uniprot ID

O95983

Antibody Clonality

Monoclonal

Application Notes

Western blot analysis: 2-4 µg/ml, Immunohistochemical analysis: 5 µg/ml, FACS analysis: 0.5 µg/10^6 cells

Storage Note

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.