Monoclonal Antibody to MBD1 (Clone: ABM15H2)
Catalogue Number: 10-7007-ABO
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Fig-5: Intracellular staining of PMA treated Jurkat cells using 0.5 µg/10^6 Cells. Green represents isotope control, Red represents 10-7007 (ABM15H2) antibody. Goat anti-mouse PE was used as secondary antibody.
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Fig-1: Western blot analysis of MBD1. Anti-MBD1 antibody (Clone: ABM15H2) was used at 2 µg/ml on HepG2, MCF7 and A431 lysates.
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Fig-2 : Immunohistochemical analysis of MBD1 in Cystadenocarcinoma of ovary using MBD1 antibody (Clone: ABM15H2) at 5 µg/ml.
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Fig-3 : Immunohistochemical analysis of MBD1 in human Kidney tissue using MBD1 antibody (Clone: ABM15H2) at 5 µg/ml.
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Fig-4 : Immunohistochemical analysis of MBD1 in Squamous cell carcinoma of Lungs using MBD1 antibody (Clone: ABM15H2) at 5 µg/ml.
| Manufacturer: | Abeomics |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | MBD1||CXXC3||PCM1 |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ug, 25 ug |
| Host name: | Mouse |
| Clone: | ABM15H2 |
| Isotype: | IgG1, kappa |
| Immunogen: | A partial length recombinant MBD1 protein (amino acids 291-586) was used as the immunogen for this antibody. |
| Application: | FACS, WB, IHC |
Description
Description: MBD1 (Methyl-CpG-Binding Domain Protein 1) protein is a primary candidate for the readout of DNA methylation as it recruits chromatin remodelers, histone deacetylases and methylases to methylated DNA associated with gene repression. This protein, a member of a transcriptional repressor family MBD, is predominantly expressed in neurons. MBD protein binding requires both functional MBD domains and methyl-CpGs; however, some MBD proteins also bind unmethylated DNA and active regulatory regions via alternative regulatory domains or interaction with the NuRD/Mi-2 (Nucleosome Remodeling Deacetylase) complex members. The CXXC3 domain of MBD1 makes it a unique member of the MBD family due to its affinity to unmethylated DNA. MBD1 acts as an epigenetic regulator via different mechanisms, such as the formation of the MCAF1/MBD1/SETDB1 complex or the MBD1-HDAC3 complex. It also plays an important role in disease progression, contributes to the drug resistance of PC cells; however, the mechanism underlying the drug resistance endowed by MBD1 remains unknown.
Additional Text
Gene Name
MBD1
Gene ID
4152
Purification
Protein G purified
Uniprot ID
Q9UIS9
Antibody Clonality
Monoclonal
Application Notes
Western blot analysis: 2-4 µg/ml, Immunohistochemical analysis: 5 µg/ml, Facs analysis:.0.5-1 µg/10^6 Cells
Storage Note
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
10-7007 Datasheet
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