Monoclonal Antibody to MBD1 (Clone: ABM15H2)

Catalogue Number: 10-7007-ABO

Manufacturer:Abeomics
Type:Monoclonal Primary Antibody - Unconjugated
Alias:MBD1||CXXC3||PCM1
Shipping Condition:Blue Ice
Storage Condition:2-8°C
Unit(s): 100 ug, 25 ug
Host name: Mouse
Clone: ABM15H2
Isotype: IgG1, kappa
Immunogen: A partial length recombinant MBD1 protein (amino acids 291-586) was used as the immunogen for this antibody.
Application: FACS, WB, IHC

Description

Description: MBD1 (Methyl-CpG-Binding Domain Protein 1) protein is a primary candidate for the readout of DNA methylation as it recruits chromatin remodelers, histone deacetylases and methylases to methylated DNA associated with gene repression. This protein, a member of a transcriptional repressor family MBD, is predominantly expressed in neurons. MBD protein binding requires both functional MBD domains and methyl-CpGs; however, some MBD proteins also bind unmethylated DNA and active regulatory regions via alternative regulatory domains or interaction with the NuRD/Mi-2 (Nucleosome Remodeling Deacetylase) complex members. The CXXC3 domain of MBD1 makes it a unique member of the MBD family due to its affinity to unmethylated DNA. MBD1 acts as an epigenetic regulator via different mechanisms, such as the formation of the MCAF1/MBD1/SETDB1 complex or the MBD1-HDAC3 complex. It also plays an important role in disease progression, contributes to the drug resistance of PC cells; however, the mechanism underlying the drug resistance endowed by MBD1 remains unknown.

Additional Text

Gene Name

MBD1

Gene ID

4152

Purification

Protein G purified

Uniprot ID

Q9UIS9

Antibody Clonality

Monoclonal

Application Notes

Western blot analysis: 2-4 µg/ml, Immunohistochemical analysis: 5 µg/ml, Facs analysis:.0.5-1 µg/10^6 Cells

Storage Note

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.