Mouse anti double-stranded RNA (K2)
Catalogue Number: 10030010-NMB
| Manufacturer: | Nordic MuBio |
| Type: | Monoclonal Primary Antibody - Unconjugated |
| Alias: | Mouse anti dsRNA |
| Shipping Condition: | RT |
| Unit(s): | 10 ml |
| Host name: | Mouse |
| Clone: | K2 |
| Isotype: | IgM, kappa |
| Immunogen: | |
| Application: | ELISA, IF, sELISA |
Description
Description: Over the past decade our double-stranded RNA (dsRNA)antibodies have been used extensively to detect and characterise plant and animal viruses with dsRNA genomes or intermediates. In addition, the anti-dsRNA antibodies can be used as a diagnostic tool to detect pathogens, including detection in paraffin-embedded fixed tissue samples (Richardson et al. 2010). K2 anti-dsRNA monoclonal antibody has an IgM isotype. K2 has primarily been used in ELISA and sandwich ELISA. Generally, K2 can be used in applications where an anti-dsRNA antibody with an isotype other than IgG (IgG2a) is required.
Additional Text
Antibody Clonality
Monoclonal
Application Notes
MAb K2 is primarily used for a sandwich ELISA to detect and quantitate (after calibration) dsRNA (see Schoenborn et al.). For this application it should be diluted with PBS. It may also be advantageous to use K2 for immunofluorescence studies. The optimum working dilution of the antibody for any specific application should be established by titration. Not for use for clinical purposes. For in vitro use only.
Storage Note
After delivery antibodies should be aliquoted and stored at -20 ° or -70 °C. After adding 10 mM sodium azide undiluted antibody can also be stored at +4 °C for a short period of time. For long term storage the mAb should be kept frozen. Repeated freezing/thawing cycles should be avoided.
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