iFluor® 350 succinimidyl ester
Catalogue Number: 1020-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Fluorescent Reagents |
| Shipping Condition: | RT |
| Unit(s): | 1 mg |
Description
Description: AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 532 dyes have fluorescence excitation and emission maxima of ~537 nm and ~560 nm respectively. These spectral characteristics make them an excellent upgrade to Alexa Fluor® 532 labeling dye (Alexa Fluor® is the trademark of Invitrogen). iFluor® 532 is perfectly excited at 532 nm with its maximum excitation at 537 nm, while Alexa Fluor® 532 has its maximum excitation at 524 nm (~8 nm off the desired 532 nm laser line). iFluor® 532 has a fluorescence quantum yield higher than Alexa Fluor® 532 by ~40% under the same conditions. In addition, iFluor® 532 is much more stable at a high pH range (e.g., 8-10, commonly used in DNA sequencing) than Alexa Fluor® 532. iFluor® 532 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
1020 Protocol
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