iFluor® 488 maleimide

Catalogue Number: 1062-AAT

Manufacturer:	AAT Bioquest
Shelf Life:	12 months
Type:	Fluorescent Reagents
Shipping Condition:	RT
Unit(s):	1 mg

Overview
Datasheets

Description

Description: Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor™ 488 dyes (Ex/Em=491 nm/520 nm) are far superior compared to conjugates of fluorescein derivatives such as FITC. iFluor™ 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor™ 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor™ 488 maleimide is reasonably stable and shows good reactivity and selectivity with thiol group. This iFluor™ 488 has spectral properties and reactivity similar to Alexa Fluor® 488 maleimide ( Alexa Fluor® is the trademark of Invitrogen).