iFluor® 488 hydrazide
Catalogue Number: 1082-AAT
| Manufacturer: | AAT Bioquest |
| Shelf Life: | 12 months |
| Type: | Fluorescent Reagents |
| Shipping Condition: | RT |
| Unit(s): | 1 mg |
Description
Description: Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor™ 488 dyes (Ex/Em=491 nm/520 nm) are far superior compared to conjugates of fluorescein derivatives such as FITC. iFluor™ 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor™ 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor™ 488 hydrazide is reasonably stable and shows good reactivity and selectivity with carbonyl group (such as aldehyde). This iFluor™ 488 has spectral properties and reactivity similar to Alexa Fluor® 488 hydrazide ( Alexa Fluor® is the trademark of Invitrogen).
Additional Text
Storage Note
Freeze (< -15 °C); Minimize light exposure;|12 months
1082 Protocol
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