Polyclonal Antibody to GAPDH
Catalogue Number: 11-13024-ABO
11-13024 Image
11-13024 Image
11-13024 Image
11-13024 Image
11-13024 Image
11-13024 Image
Figure-1: Western blot analysis of GAPDH. Anti- GAPDH antibody (11-13024) was used at 1 µg/ml on (1) h Brain, (2) h Heart, (3) h Small Intestine, (4) h Kidney, (5) h Liver, (6) h Lung, (7) h Skeletal Muscle, (8) h Stomach, (9) h Spleen, (10) h Ovary, (11) h Testis lysates.
11-13024 Image
Figure-2: Western blot analysis of GAPDH. Anti- GAPDH antibody (11-13024) was used at 1 µg/ml on (1) r Brain, (2) r Heart, (3) r Kidney, (4) r Liver, (5) r Lung, (6) r Skeletal Muscle, (7) r Stomach, (8) r Spleen, (9) r Ovary, (10) r Testis lysates.
11-13024 Image
Figure-3: Western blot analysis of GAPDH. Anti- GAPDH antibody (11-13024) was used at 1 µg/ml on (1) m Kidney, (2) m Liver, (3) m Lung, (4) m Skeletal Muscle and (5) m Testis lysates.
11-13024 Image
Figure-4: Western blot analysis of GAPDH. Anti- GAPDH antibody (11-13024) was used at 1 µg/ml on (1) 3T3, (2) Hela, (3) Jurkat, (4) PC3, (5) HCT-116, (6) Snu1 and (7) PANC-1 lysates.
11-13024 Image
Figure-5: Western blot analysis of GAPDH. Anti- GAPDH antibody (11-13024) was used at 1 µg/ml on (1) K562, (2) A549, (3) MCF-7, (4) 293, (5) A431 and (6) HepG2 lysates.
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | GAPDH, GAPD, CDABP0047, OK/SW-cl.12 |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ug, 25 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | A partial length recombinant GAPDH protein was used as the immunogen for this antibody. |
| Application: | WB |
Description
Description: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) is an enzyme best known for its role in glycolysis. However, extra-glycolytic functions of GAPDH have been described, including regulation of protein expression via RNA binding. GAPDH binds to numerous AREs (adenine-uridine rich elements) from various mRNA 3'-untranslated regions in vitro and in vivo despite its lack of a canonical RNA binding motif. GAPDH specifically catalyzes the simultaneous phosphorylation and oxidation of glyceraldehyde 3-phosphate using NAD+ (Nicotinamide Adenine Dinucleotide) as a cofactor to produce glycerate 1,3-biphosphate and NADH. In addition to its role in energy production, GAPDH has been implicated in many cellular processes including DNA repair tRNA export, membrane fusion and transport, endocytosis and nuclear membrane assembly, and cell death.
Additional Text
Gene Name
GAPDH
Uniprot ID
P04406
Gene ID
2597
Purification
Protein A purified
Antibody Clonality
Polyclonal
Application Notes
Western blot analysis: 1-2 µg/ml
Storage Note
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
11-13024 Datasheet
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