Polyclonal Antibody to GAPDH

Catalogue Number: 11-13024-ABO

Manufacturer:Abeomics
Type:Polyclonal Primary Antibody - Unconjugated
Alias:GAPDH, GAPD, CDABP0047, OK/SW-cl.12
Shipping Condition:Blue Ice
Storage Condition:2-8°C
Unit(s): 100 ug, 25 ug
Host name: Rabbit
Clone:
Isotype: IgG
Immunogen: A partial length recombinant GAPDH protein was used as the immunogen for this antibody.
Application: WB

Description

Description: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) is an enzyme best known for its role in glycolysis. However, extra-glycolytic functions of GAPDH have been described, including regulation of protein expression via RNA binding. GAPDH binds to numerous AREs (adenine-uridine rich elements) from various mRNA 3'-untranslated regions in vitro and in vivo despite its lack of a canonical RNA binding motif. GAPDH specifically catalyzes the simultaneous phosphorylation and oxidation of glyceraldehyde 3-phosphate using NAD+ (Nicotinamide Adenine Dinucleotide) as a cofactor to produce glycerate 1,3-biphosphate and NADH. In addition to its role in energy production, GAPDH has been implicated in many cellular processes including DNA repair tRNA export, membrane fusion and transport, endocytosis and nuclear membrane assembly, and cell death.

Additional Text

Gene Name

GAPDH

Uniprot ID

P04406

Gene ID

2597

Purification

Protein A purified

Antibody Clonality

Polyclonal

Application Notes

Western blot analysis: 1-2 µg/ml

Storage Note

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.