Coronavirus (COVID-19) Nucleocapsid Antibody
Catalogue Number: 11-2003-ABO
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11-2003 Image
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Figure-1: Wells of a 96-microtiter plate were coated with 4ug/ml of SARS-Cov-2/nCov/COVID-19 Nucleocapsid recombinant protein (21-1003). The binding was detected by addition of different dilution of 11-2003 polyclonal antibody. The reactivity was detected by a HRP-conjugated goat-anti-rabbit polyclonal antibody.
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Figure-2: Western Blot analysis of SARS-CoV-2 Nucleocapsid Protein: Anti- SARS-CoV-2 Nucleocapsid Protein (11-2003) was used at 2 µg/ml on (1) SARS-CoV-2 virus infected Vero Cell lysate and (2) Mock infected lysate.
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Figure-3: Western Blot analysis of Nucleocapsid antibody: Anti- Nucleocapsid antibody (SARS-CoV-2) was used at 4 µg/ml on recombinant Nucleocapsid Protein (21-1003).
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 25 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | A partial length recombinant coronavirus Nucleocapsid protein (amino acids 250-410) was used as the immunogen for this antibody. |
| Application: | ELISA, WB |
Description
Description: The structural nucleocapsid (N) protein of nCoV/SARS-CoV-2/COVID-19 is a predicted 46 kDa phosphoprotein having 419 amino acid residues. A short Serine rich stretch and a recognized nuclear localization signal are the unique features of the nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19, which seems to have a little homology with the proteins of other members of this large corona virus family. These features also suggest the involvement of nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 in different stages of viral lifecycle. The protein has multifaceted activities including packing of the nCoV/SARS-CoV-2/COVID-19 viral genome into a helical ribonucleocapsid (RNP) and playing an important role in viral self-assembly causing nCoV/SARS-CoV-2/COVID-19. The nucleocapsid protein of nCoV/SARS-CoV-2/COVID-19 also forms dimer in the cell by self-association with the help of interactive C terminal domain.
Additional Text
Purification
Protein A purified
Antibody Clonality
Polyclonal
Application Notes
Recommended dilutions: WB: 0.5-1 µg/ml. However, this need to be optimized based on the research applications.
Storage Note
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
11-2003 Datasheet
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