Polyclonal Antibody to HMGB1

Catalogue Number: 11-3045-ABO

Manufacturer:Abeomics
Type:Polyclonal Primary Antibody - Unconjugated
Alias:HMGB1||HMG1
Shipping Condition:Blue Ice
Storage Condition:2-8°C
Unit(s): 100 ug, 25 ug
Host name: Rabbit
Clone:
Isotype: IgG
Immunogen: A partial length recombinant HMGB1 protein (amino acids 1-210) was used as the immunogen for this antibody.
Application: WB

Description

Description: HMGB1 (High-mobility group protein B1) is a 25 kDa non-histone chromatin protein, secreted by immune cells through leaderless secretory pathway. HMGB1 supports transcription and also interacts with nucleosomes to loosen packed DNA and remodel the chromatin. The presence of HMGB1 in the nucleus depends on posttranslational modifications. When the protein is not acetylated, it stays in the nucleus, but hyperacetylation on lysine residues causes it to translocate into the cytosol. HMGB1 has the ability to bend DNA in order to facilitate binding of other proteins to the DNA. HMGB1 has one important role in V(D)J recombination where act as a cofactor of the RAG complex. HMGB1 stimulates a plethora of cellular responses like inflammation, attracting inflammatory cells, recruiting stem cells and promoting their proliferation. HMGB1 is highly expressed in human kidney, tonsils, adipose tissue, adrenal cortex and amniotic fluid.

Additional Text

Gene ID

3146

Gene Name

HMGB1

Uniprot ID

P09429

Purification

Protein A purified

Antibody Clonality

Polyclonal

Application Notes

Western blot analysis: 1-2 µg/ml

Storage Note

Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.