Polyclonal Antibody to ICAD
Catalogue Number: 11-7002-ABO
| Manufacturer: | Abeomics |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Alias: | DFFA||DFF1||DFF45||H13 |
| Shipping Condition: | Blue Ice |
| Storage Condition: | 2-8°C |
| Unit(s): | 100 ug, 25 ug |
| Host name: | Rabbit |
| Clone: | |
| Isotype: | IgG |
| Immunogen: | A partial length recombinant ICAD protein (amino acids 111-331) was used as the immunogen for this antibody. |
| Application: | WB |
Description
Description: ICAD/DFF45 is an inhibitor of the caspase-activated DNase (CAD, DFF40). DNA fragmentation factor (DFF) consists of two subunits, a 40 kDa caspase-activated nuclease (DFF40) and a 45 kDa inhibitor (DFF45). In proliferating cells CAD or DFF40 forms a complex with ICAD or DFF45. When cells undergo apoptosis, caspase 3 cleaves ICAD at two sites to dissociate the CAD:ICAD complex, allowing CAD to cleave chromosomal DNA. Thus DEF induces the fragmentation of DNA which is a hallmark of apoptosis. ICAD is expressed in brain, blood, skin, spleen, adipose tissue. It has 2 isoforms produced by alternative splicing.
Additional Text
Gene ID
1676
Gene Name
DFFA
Uniprot ID
O00273
Purification
Protein A purified
Antibody Clonality
Polyclonal
Application Notes
Western blot analysis: 4-6 µg/ml
Storage Note
Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
11-7002 Datasheet
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