Anti-Microtubule Associated Protein 2 (MAP2) Antibody
Catalogue Number: 1100-MAP2-PPS
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Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-nestin antibody (cat. 1435-NES, red, 1:500) and anti-MAP2 antibody (cat. 1100-MAP2, green, 1:500). The blue is Hoechst staining for nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.
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Immunofluorescence of cortical neuron-glial cell culture from E20 rat labeled with Anti- MAP2(cat. 1100-MAP2, red, 1:10,000), and Anti-Tau (green). The blue is DAPI staining of nuclear DNA. The anti-MAP2 antibody stains dendrites and perikarya of neurons, while the anti-TAU antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.
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(A-B). Primary hippocampal neurons were stained with MAP2 (cat. 1100-MAP2, 1:1000) as a negative control under basal conditions (CTRL) and with picrotoxin treatment (PTX). Image from publication CC-BY-4.0. PMID: 37654026
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Day 14 (D14) RGC-iNs stained with a DAPI, TAU, and MAP2 (cat. 1100-MAP2, 1:1000). Image from publication CC-BY-4.0. PMID: 37773257
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Immunolabeling of mouse hippocampal cultures labeled with anti-MAP2 (cat. 1100-MAP2, 1:1000, red) and anti-synapsin (cat. 1925-SYNP , 1:1000, green).
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Costaining of the primary brain cells isolated from 18-day embryonic rats (E18). Neurons stained with α-MAP2 (green). Copurified astrocytes stained with α-GFAP (red). Image from publication CC-BY-4.0. PMID: 35652638
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Immunolabeling of mouse hippocampal cultures labeled with Anti-GFAP (cat. 620-GFAP, 1:1000, green) and anti-MAP2 (1100-MAP2, 1:1000, red).
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Immunolabeling of mouse cortical cultures labeled with Anti-GFAP (cat. 620-GFAP, 1:1000, green) and anti-MAP2 (1100-MAP2, 1:1000, red).
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Representative images of anti-MAP2 (ca. 1100-MAP2) immunolabeled rat neurons at 5 DIV of T30 treatment. Image from publication CC-BY-4.0. PMID: 37454238
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Immunofluorescence of a section of rat brain stem showing colabeled with Anti-MAP2 (cat. 1100-MAP2, red, 1:5000) and Anti-FOX3( cat. 583-FOX3 , green, 1:1000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.
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Immunostaining of rat hippocampus showing specific immunolabeling of MAP2 (cat. 1100-MAP2, red, 1:1000), Tau-1 (green), and nuclear DNA labeled with DAPI (blue). Photo courtesy of the Miriam Zamorano, University of Texas Health Science Center at Houston.
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Immunofluorescence of rat cerebellum showing strong synaptic staining of VSNL1 (cat #: 2145-VSNL1, green) in the molecular layer (ML) and MAP2 (cat # 1100-MAP2, red, 1:2500). The blue stain is nuclear DNA.
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Western blot of rat cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.
1100-MAP2 Image
Immunolabeling of mouse hippocampal cultures labeled with anti-MAP2 (cat. 1100-MAP2, 1:1000, red) and anti-synapsin (cat. 1925-SYNP , 1:1000, green).
1100-MAP2 Image
Immunofluorescence of cortical neuron-glial cell culture from E20 rat labeled with Anti- MAP2(cat. 1100-MAP2, red, 1:10,000), and Anti-Tau (green). The blue is DAPI staining of nuclear DNA. The anti-MAP2 antibody stains dendrites and perikarya of neurons, while the anti-TAU antibody labels neuronal perikarya, dendrites and also axonal process. As a result perikarya and dendrites appears orange-yellow, since they contain both MAP2 and tau, while axons are green.
1100-MAP2 Image
Western blot of rat cortical lysate showing specific immunolabeling of the ~280 kDa MAP2 protein.
1100-MAP2 Image
Immunofluorescence of a section of rat brain stem showing colabeled with Anti-MAP2 (cat. 1100-MAP2, red, 1:5000) and Anti-FOX3( cat. 583-FOX3 , green, 1:1000). The Anti-FOX3 specifically labels the nuclei and the proximal cytoplasm of neuronal cells while the Anti-MAP2 labels dendrites and overlaps with FOX3 labeling the perikarya of neurons. The blue is DAPI staining of nuclear DNA.
| Manufacturer: | Phosphosolutions |
| Preservative: | 10 mM Sodium azide |
| Physical state: | Liquid |
| Type: | Polyclonal Primary Antibody - Unconjugated |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ul, 25 ul |
| Host name: | Chicken |
| Clone: | |
| Isotype: | IgY |
| Immunogen: | Recombinant bovine MAP2 protein expressed in and purified from E. Coli. |
| Application: | ICC, WB, IHC |
Additional Text
Gene Name
MAP2
Gene ID
4133
Uniprot ID
P11137
Molecular Weight
280
Antibody Clonality
Polyclonal
Short Description
Our Anti-Microtubule Associated Protein 2 (MAP2) primary antibody from PhosphoSolutions is chicken polyclonal. It detects bovine, human, mouse, and rat Microtubule Associated Protein 2 (MAP2) and is total IgY fraction. It is great for use in WB, IHC, ICC.
Specificity
DKFZp686I2148 antibody, MAP 2 antibody, MAP dendrite specific antibody, MAP-2 antibody, MAP2 antibody, MAP2A antibody, MAP2B antibody, MAP2C antibody, Microtubule associated protein 2 antibody, Microtubule-associated protein 2 antibody, MTAP2_HUMAN antibody
Storage Note
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
1100-MAP2 Datasheet
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DELIVERY
we offer free delivery to UK universities and non profit organisations