Amplite® Red

Catalogue Number: 11011-AAT

Manufacturer:AAT Bioquest
Shelf Life:12 months
Type:Fluorophores
Shipping Condition:RT
Unit(s): 1000 Assays

Description

Description: Our Amplite™ Red is a sensitive fluorogenic peroxidase substrate that generates a highly red fluorescent product that has maximum absorption of 571 nm and maximum emission of 585 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of Amplite™ Red is minimal. Amplite™ Red is one of the most sensitive and stable fluorogenic probes for detecting HRP and H2O2. Amplite™ Red has been widely used to detect HRP in many immunoassays. On the other hand, Amplite™ Red can also be used to detect trace amount of H2O2. The Amplite™ Red-based H2O2 detection is at least one order of magnitude more sensitive than the commonly used scopoletin assay for H2O2. Because H2O2 is produced in many enzymatic redox reactions, Amplite™ Red can be used in coupled enzymatic reactions to detect the activity of many oxidases and/or related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol, L-glutamate, amino acids, etc.

Additional Text

Storage Note

Freeze (< -15 °C); Minimize light exposure; Desiccated;|12 months