Catalogue Number: 1149-AAT
Manufacturer: | AAT Bioquest |
Shelf Life: | 12 months |
Type: | Fluorescent Reagents |
Shipping Condition: | RT |
Unit(s): | 1 mg |
Description: AAT Bioquest's mFluor™ dyes are developed for multicolor flow cytometry-focused applications. These dyes have large Stokes Shifts, and can be well excited by the laser lines of flow cytometers (e.g., 405 nm, 488 nm and 633 nm). mFluor™ Violet 500 dyes have fluorescence excitation and emission maxima of ~405 nm and ~500 nm respectively. These spectral characteristics make them an excellent replacement for Pacific Green™ (ThermoFisher) and BD Horizon™ V500 labeling dyes. mFluor™ Violet 500 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups. mFluor™ Violet 500 SE provides a convenient tool to label monoclonal, polyclonal antibodies or other proteins (>10 kDa) for flow cytometric applications with the violet laser excitation. Under the same conditions, mFluor™ Violet 500 dye conjugates are significantly brighter than the corresponding bioconjugates of Pacific Green™ (ThermoFisher) and BD Horizon™ V500 with much stronger absorption, making the mFluor™ Violet 500 conjugates much more sensitive.
Freeze (< -15 °C); Minimize light exposure;|12 months