Peroxidase-conjugated AffiniPure™ Goat Anti-Mouse IgG, Fcγ Subclass 2b Specific (min X Hu,Bov,Rb Sr Prot)

Catalogue Number: 115-035-207-JIR

Manufacturer:	Jackson ImmunoResearch
Shelf Life:	12 months
Preservative:	No preservative
Physical state:	Freeze-dried solid
Type:	Polyclonal Secondary Antibody - Conjugated
Shipping Condition:	RT
Unit(s):	0.5 ml
Host name:	Goat
Clone:
Isotype:
Immunogen:	IgG, Fcγ subclass 2b
Application:	ELISA, ICC, WB, IHC

Overview
Datasheets

Description

Description: Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on anitgen-binding assay and/or ELISA, the antibody reacts with the Fc portion of mouse IgG2b but not with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. The antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with human, bovine and rabbit serum proteins, but it may cross-react with immunoglobulins from other species.

Additional Text

Purification

AffiniPure

Antibody Clonality

Polyclonal

Short Description

Goat Anti-Mouse IgG, Fcγ subclass 2b secondary antibody, conjugated to Horseradish Peroxidase. This is an affinity purified whole IgG polyclonal antibody and has been tested and/or adsorbed to ensure minimal cross reactivity with Human, Bovine and Rabbit serum proteins.

Application Notes

1:500 - 1:5,000 for immunohisto/cytochemistry 1:5,000 - 1:100,000 for ELISA and Western blotting with chromogenic substrates 1:10,000 - 1:200,000 for Western blotting with ECL substrates Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.