Alexa Fluor® 680-conjugated AffiniPure™ Goat Anti-Mouse IgG, Light Chain* Specific (min X Bov,Gt,Hrs,Hu,Rb,Rat,Shp Ig)

Catalogue Number: 115-625-174-JIR

Manufacturer:	Jackson ImmunoResearch
Shelf Life:	12 months
Preservative:	0.05% Sodium azide
Physical state:	Freeze-dried solid
Type:	Polyclonal Secondary Antibody - Conjugated
Shipping Condition:	RT
Unit(s):	0.3 mg
Host name:	Goat
Clone:
Isotype:
Immunogen:	IgG, light chain

Overview
Datasheets

Description

Description: Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Based on antigen-binding assay, Western blotting, and/or ELISA, the antibody reacts with the light chains on mouse IgG and with those common to other mouse immunoglobulins. Reaction is primarily with kappa light chains. The antibody does not react with the heavy chain of mouse IgG. The antibody has been tested by ELISA to ensure minimal cross-reaction with bovine, goat, horse, human, rabbit, rat and sheep immunoglobulins, but it may cross-react with immunoglobulins from other species.

Additional Text

Purification

AffiniPure

Antibody Clonality

Polyclonal

Short Description

Goat Anti-Mouse IgG, light chain secondary antibody, conjugated to Alexa Fluor® 680. This is an affinity purified whole IgG polyclonal antibody and has been tested and/or adsorbed to ensure minimal cross reactivity with Bovine, Goat, Horse, Human, Rabbit, Rat and Sheep serum proteins.

Application Notes

Western Blot:- 1:50,000-200,000 Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.