Catalogue Number: 115-657-185-JIR
Manufacturer: | Jackson ImmunoResearch |
Shelf Life: | 12 months |
Preservative: | 0.05% Sodium azide |
Physical state: | Freeze-dried solid |
Type: | Polyclonal Secondary Antibody - Conjugated |
Alias: | IgG1 |
Shipping Condition: | RT |
Unit(s): | 0.5 mg |
Host name: | Goat |
Clone: | |
Isotype: | |
Immunogen: | IgG1, Fcγ fragment |
Application: | Lbl |
Description: Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa. Based on antigen-binding assay and/or ELISA, the antibody reacts with the Fc portion of mouse IgG1 but not with the Fab portion of mouse immunoglobulins. No antibody was detected against mouse IgM or against non-immunoglobulin serum proteins. The antibody may cross-react with other mouse IgG subclasses or with immunoglobulins from other species.
AffiniPure
Polyclonal
Goat Anti-Mouse IgG1, Fcγ fragment secondary antibody, conjugated to Alexa Fluor® 790. This is an affinity purified Fab Fragment polyclonal antibody.
1:100 - 1:800 for sequential labeling applications. To complex with primary antibody in solution, use 1:1 weight ratio of Fab:primary antibody (3:1 molar ratio). Vortex and incubate for 30 minutes at room temperature prior to use. Titrate complex to optimal dilution for assay. Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
Store freeze-dried solid at 2-8°C.|One year from date of rehydration.