Recombinant Mouse Monoclonal Antibody to Calponin-1 (Smooth Muscle Marker)(Clone : rCNN1/832)

Catalogue Number: 12-1001-ABO

Manufacturer:	Abeomics
Type:	Recombinant Monoclonal
Alias:	Calponin 1 basic smooth muscle, Calponin H1 smooth muscle, Calponin-1, CNN1, Cnn1, Sm Calp, SMCC
Shipping Condition:	Blue Ice
Unit(s):	100 ug, 20 ug
Host name:
Clone:	rCNN1/832
Isotype:	IgG1, kappa
Immunogen:	Recombinant full-length human CNN1 protein
Application:	FACS, WB, IHC

Overview
Datasheets

Description

Description: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

Additional Text

Gene Name

CNN1

Gene ID

1264

Uniprot ID

P51911

Purification

Purified

Antibody Clonality

Monoclonal

Application Notes

Western Blot (1-2µg/ml); Flow Cytometry (1-2µg/million cells); Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)

Storage Note

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.

12-1001 Datasheet

Price

£533.00

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