Recombinant Mouse Monoclonal Antibody to Histone H1 (Nuclear Marker)(Clone : r1415-1)
Catalogue Number: 12-1004-ABO
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12-1004 Image
12-1004 Image
12-1004 Image
Figure 1: Formalin-fixed, paraffin-embedded human Tonsil stained with Histone H1 Mouse Recombinant Monoclonal Antibody (r1415-1).
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Figure 3: SDS-PAGE Analysis of Purified Histone H1 Mouse Recombinant Monoclonal Antibody (r1415-1).
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Figure 2: Formalin-fixed, paraffin-embeddedRatPancreas stained with Histone H1 Mouse Recombinant Monoclonal Antibody (r1415-1).
| Manufacturer: | Abeomics |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 20 ug |
| Host name: | |
| Clone: | r1415-1 |
| Isotype: | IgG2a, kappa |
| Immunogen: | Nuclei of human leukemia biopsy cells |
| Application: | FACS, IF, WB, IHC |
Description
Description: Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
Additional Text
Uniprot ID
P07305
Gene ID
3005
Specificity
H1(0), H1.1, H1.2, H1.3, H1.4, H1.5, H10, H1A, H1F0, H1F1, H1F2, H1F3, H1F4, H1F5, H1FNT, H1FOO, H1FT, H1FV, H1FX, H1t, H1T2, H1X, HANP1, His1, HisC, HIST1, HIST1H1A, HIST1H1B, HIST1H1C, HIST1H1D, HIST1H1E, HIST1H1T, Oocyte-specific histone H1, Testicular H1 histone
Purification
Purified
Antibody Clonality
Monoclonal
Gene Name
H1-0
Application Notes
Western Blot (1-2µg/ml); Flow Cytometry (1-2µg/million cells); Immunofluorescence (1-2µg/ml); Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Storage Note
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
12-1004 Datasheet
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