Recombinant Mouse Monoclonal Antibody to Cytokeratin 7 (Glandular and Transitional Epithelial Marker)(Clone : rOV-TL12/30)

Catalogue Number: 12-1005-ABO

Manufacturer:Abeomics
Type:Recombinant Monoclonal
Alias:CK-7, K2C7, Keratin 55K Type II Cytoskeletal, Keratin Simple Epithelial Type 1 K7, Keratin Type II Cytoskeletal 7, Krt2-7, KRT7, Sarcolectin, SCL, Type II Mesothelial Keratin K7, Type-II Keratin Kb7
Shipping Condition:Blue Ice
Unit(s): 100 ug, 20 ug
Host name:
Clone: rOV-TL12/30
Isotype: IgG1, kappa
Immunogen: OTN 11, ovarian carcinoma cell line
Application: FACS, WB, IHC

Description

Description: It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).

Additional Text

Gene Name

KRT7

Gene ID

3855

Uniprot ID

P08729

Purification

Purified

Antibody Clonality

Monoclonal

Application Notes

Western Blot (1-2µg/ml); Flow Cytometry (1-2µg/million cells); Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)

Storage Note

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.