Recombinant Mouse Monoclonal Antibody to Ep-CAM / CD326 (Epithelial Marker)(Clone : rMOC-31)

Catalogue Number: 12-1006-ABO

Manufacturer:Abeomics
Type:Recombinant Monoclonal
Shipping Condition:Blue Ice
Unit(s): 100 ug, 20 ug
Host name:
Clone: rMOC-31
Isotype: IgG1, kappa
Immunogen: Neuraminidase treated GLS-1 human small cell lung carcinoma cells
Application: FACS, IF, WB, IHC

Description

Description: Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.

Additional Text

Gene ID

4072

Gene Name

EPCAM

Uniprot ID

P16422

Specificity

Adenocarcinoma-associated Antigen, Cell Surface Glycoprotein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial Glycoprotein 314, ESA, KSA, TACD1, TROP1, Tumor-associated Calcium Signal Transducer 1 (TACSTD1), ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, F

Purification

Purified

Antibody Clonality

Monoclonal

Application Notes

Flow Cytometry (1-2µg/million cells); Immunofluorescence (1-2µg/ml); Western Blot (1-2µg/ml); ,Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)

Storage Note

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.