Recombinant Mouse Monoclonal Antibody to MUC1 / EMA / CD227 (Epithelial Marker)(Clone : rMUC1/960)
Catalogue Number: 12-1008-ABO
12-1008 Image
12-1008 Image
12-1008 Image
Figure 2: SDS-PAGE Analysis of Purified MUC1 Mouse Recombinant Monoclonal Antibody (rMUC1/960).
12-1008 Image
Figure 1: Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC1 Mouse Recombinant Monoclonal Antibody (rMUC1/960).
| Manufacturer: | Abeomics |
| Type: | Recombinant Monoclonal |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 20 ug |
| Host name: | |
| Clone: | rMUC1/960 |
| Isotype: | IgG2b, kappa |
| Immunogen: | Human milk-fat globule membranes (HMFGM) |
| Application: | FACS, IF, WB, IHC |
Description
Description: This MAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Neuraminidase treatment destroys the antigen. It is a very good tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion.
Additional Text
Gene Name
MUC1
Gene ID
4582
Uniprot ID
P15941
Specificity
Breast carcinoma-associated antigen DF3, CA15-3, Carcinoma-associated mucin Episialin, Epithelial Membrane Antigen, H23AG, KL-6, MAM6, MUC-1, MUC-1/SEC, MUC-1/X, MUC1-alpha, MUC1-beta, MUC1-CT, MUC1-NT, MUC1/ZD, Mucin 1 cell surface associated, Mucin-1 subunit beta, Peanut-reactive
Purification
Purified
Antibody Clonality
Monoclonal
Application Notes
Flow Cytometry (1-2µg/million cells); Immunofluorescence (1-2µg/ml); Western Blot (1-2µg/ml); ,Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Storage Note
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
12-1008 Datasheet
SUPPORT
outstanding technical support
PRODUCT
we offer a full product guarantee
DELIVERY
we offer free delivery to UK universities and non profit organisations