Recombinant Mouse Monoclonal Antibody to Wilm's Tumor 1 (WT1) (Wilm's Tumor & Mesothelial Marker)(Clone : rWT1/857)
Catalogue Number: 12-1011-ABO
12-1011 Image
12-1011 Image
12-1011 Image
Figure 2: SDS-PAGE Analysis of Purified Wilm's Tumor Mouse Recombinant Monoclonal Antibody (rWT1/857).
12-1011 Image
Figure 1: Formalin-fixed, paraffin-embedded Human Kidney stained with Wilm's Tumor Mouse Recombinant Monoclonal Antibody (rWT1/857).
| Manufacturer: | Abeomics |
| Type: | Recombinant Monoclonal |
| Alias: | WT1, AWT1, FWT1, GUD, NPHS4, WAGR, Wilms tumor 1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 20 ug |
| Host name: | |
| Clone: | rWT1/857 |
| Isotype: | IgG1, kappa |
| Immunogen: | Recombinant full-length human WT1 protein |
| Application: | IHC |
Description
Description: Recognizes a 47-55kDa-tumor suppressor protein, identified as Wilm's Tumor (WT1) protein. The antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids, frequently found in subsets of sporadic Wilm s tumors. WT1, a sporadic and familial pediatric kidney tumor, is genetically heterogeneous. Wilm s tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The WT1 gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilm s tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.
Additional Text
Gene Name
WT1
Gene ID
7490
Uniprot ID
P19544
Purification
Purified
Antibody Clonality
Monoclonal
Application Notes
Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Storage Note
Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
12-1011 Datasheet
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