Recombinant Mouse Monoclonal Antibody to CD20 / MS4A1 (B-Cell Marker)(Clone : rIGEL/773)

Catalogue Number: 12-1012-ABO

Manufacturer:Abeomics
Type:Recombinant Monoclonal
Shipping Condition:Blue Ice
Unit(s): 100 ug, 20 ug
Host name:
Clone: rIGEL/773
Isotype: IgG1, kappa
Immunogen: Recombinant full-length human MS4A1 protein
Application: FACS, IF, IHC

Description

Description: Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.

Additional Text

Gene ID

931

Gene Name

MS4A1

Uniprot ID

P11836

Specificity

APY, ATOPY, B-lymphocyte antigen CD20, B-lymphocyte cell-surface antigen B1, Bp35, Fc epsilon receptor I beta chain, Fc Fragment of IgE high affinity I receptor for beta polypeptide, FCER1B, High affinity immunoglobulin epsilon receptor subunit beta, IgE Fc receptor subunit beta, IGEL, IG

Purification

Purified

Antibody Clonality

Monoclonal

Application Notes

Immunofluorescence (1-2µg/ml); Flow Cytometry (1-2µg/million cells);Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)

Storage Note

Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.