Anti-Beta-2 Microglobulin (Renal Failure &Tumor Marker) Recombinant Mouse Monoclonal Antibody (Clone:rB2M/961)

Catalogue Number: 12-1083-ABO

Manufacturer:Abeomics
Shelf Life:24 months
Type:Recombinant Monoclonal
Alias:B2M; Beta 2 microglobin; Beta 2 microglobulin; Beta chain of MHC class I molecules; Beta-2-microglobulin form pI 5.3; Hdcma22p
Shipping Condition:Blue Ice
Unit(s): 100 ug, 20 ug
Host name: Mouse
Clone: rB2M/961
Isotype: IgG2b, kappa
Immunogen: Recombinant Full length human B2M protein
Application: FACS, IF, WB, IHC

Description

Description: Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alphaheavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alphaheavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alphaheavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.

Additional Text

Gene Name

B2M

Purification

Protein A/G Purified

Gene ID

567

Uniprot ID

P61769

Antibody Clonality

Monoclonal

Application Notes

Flow Cytometry (1-2µg/million cells); Immunofluorescence (1-2µg/ml); Western Blot (1-2µg/ml); Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)

Storage Note

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.