Anti-Catenin, beta (CTNNB1) Recombinant Mouse Monoclonal Antibody (Clone:rCTNNB1/2173)
Catalogue Number: 12-1092-ABO
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12-1092 Image
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Figure 1: Formalin-fixed, paraffin-embedded human Breast Cancer stained with Beta-Catenin Mouse Recombinant Monoclonal Antibody (rCTNNB1/2173).
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Figure 2: SDS-PAGE Analysis of Purified Beta-Catenin Mouse Recombinant Monoclonal Ab (rCTNNB1/2173).
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| Manufacturer: | Abeomics |
| Shelf Life: | 24 months |
| Type: | Recombinant Monoclonal |
| Alias: | Cadherin associated protein, beta 1 88kDa, Catenin beta-1, CATNB, CHBCAT, CTNNB1 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 20 ug |
| Host name: | Mouse |
| Clone: | rCTNNB1/2173 |
| Isotype: | IgG1, kappa |
| Immunogen: | Recombinant full-length human CCNB1 protein |
| Application: | IF, WB, IHC |
Description
Description: Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throµghout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.
Additional Text
Gene Name
CTNNB1
Gene ID
1499
Purification
Protein A/G Purified
Uniprot ID
P35222
Antibody Clonality
Monoclonal
Application Notes
Immunofluorescence (1-2µg/ml); Western Blot (1-2µg/ml); ,Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Storage Note
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
12-1092 Datasheet
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