Anti-CD43 (T-Cell Marker) Recombinant Mouse Monoclonal Antibody (Clone:rSPN/839)
Catalogue Number: 12-1113-ABO
| Manufacturer: | Abeomics |
| Shelf Life: | 24 months |
| Type: | Recombinant Monoclonal |
| Alias: | Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 20 ug |
| Host name: | Mouse |
| Clone: | rSPN/839 |
| Isotype: | IgG1, kappa |
| Immunogen: | Human full-length recombinant CD43 protein |
| Application: | FACS, IF, WB, IHC |
Description
Description: It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 [Workshop IV]. Epitope of MAb Bra7G is clearly different from that of MAb rSPN/839, called "b" as opposed to "a" for rSPN/839. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Additional Text
Gene Name
SPN
Purification
Protein A/G Purified
Gene ID
6693
Uniprot ID
P16150
Antibody Clonality
Monoclonal
Application Notes
Flow Cytometry (1-2µg/million cells);,Western Blot (1-2µg/ml);,Immunofluorescence (1-2µg/ml);,Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Storage Note
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
12-1113 Datasheet
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