Anti-CD43 (T-Cell Marker) Recombinant Mouse Monoclonal Antibody (Clone:rSPN/1094)

Catalogue Number: 12-1114-ABO

Manufacturer:Abeomics
Shelf Life:24 months
Type:Recombinant Monoclonal
Alias:Galactoglycoprotein, GALGP, GPL115, Leukocyte sialoglycoprotein, Leukosialin, LSN, Sialophorin, SPN
Shipping Condition:Blue Ice
Unit(s): 100 ug, 20 ug
Host name: Mouse
Clone: rSPN/1094
Isotype: IgG1, kappa
Immunogen: Human full-length recombinant CD43 protein
Application: FACS, IF, WB, IHC

Description

Description: It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43. 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.

Additional Text

Gene Name

SPN

Purification

Protein A/G Purified

Gene ID

6693

Uniprot ID

P16150

Antibody Clonality

Monoclonal

Application Notes

Western Blot (1-2µg/ml); Flow Cytometry (1-2µg/million cells); Immunofluorescence (1-2µg/ml); Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)

Storage Note

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.