Anti-CD8A (Cytotoxic-&Suppressor T-Cell Marker) Recombinant Mouse Monoclonal Antibody (Clone:rC8/468)
Catalogue Number: 12-1133-ABO
| Manufacturer: | Abeomics |
| Shelf Life: | 24 months |
| Type: | Recombinant Monoclonal |
| Alias: | CD8 antigen, alpha polypeptide (p32), T8/Leu-2 T-lymphocyte differentiation antigen, Ly3, LYT3, MAL, T-cell surface glycoprotein CD8 alpha chain |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 20 ug |
| Host name: | Mouse |
| Clone: | rC8/468 |
| Isotype: | IgG1, kappa |
| Immunogen: | Recombinant full-length human CD8 protein |
| Application: | FACS, IF, IHC |
Description
Description: CD8 is a cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and throµgh its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells. For mature T-cells, CD4 and CD8 are mutually exclusive, so anti-CD8, generally used in conjunction with anti-CD4.It is a useful marker for distinguishing helper/inducer T-lymphocytes, and most peripheral T-cell lymphomas are CD4+/CD8-. Anaplastic large cell lymphoma is usually CD4+ and CD8-, and in T-lymphoblastic lymphoma/leukemia, CD4 and CD8 are often co-expressed. CD8 is also found in littoral cell angioma of the spleen.
Additional Text
Gene ID
925
Gene Name
CD8A
Uniprot ID
P01732
Purification
Protein A/G Purified
Antibody Clonality
Monoclonal
Application Notes
Flow Cytometry (1-2µg/million cells); Immunofluorescence (1-2µg/ml); ,Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes)
Storage Note
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
12-1133 Datasheet
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