Anti-Cytokeratin 8 (KRT8) Recombinant Mouse Monoclonal Antibody (Clone:rB22.1)
Catalogue Number: 12-1160-ABO
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Figure 1: Formalin-fixed, paraffin-embedded human Colon stained with Cytokeratin 8 Mouse Recombinant Monoclonal Antibody (rB22.1).
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Figure 2: SDS-PAGE Analysis of Purified Cytokeratin 8 Mouse Recombinant Monoclonal Antibody (rB22.1).
| Manufacturer: | Abeomics |
| Shelf Life: | 24 months |
| Type: | Recombinant Monoclonal |
| Alias: | CARD2; CK8; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8 |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 20 ug |
| Host name: | Mouse |
| Clone: | rB22.1 |
| Isotype: | IgG1, kappa |
| Immunogen: | Cytoskeletal preparation from HeLa cells |
| Application: | WB, IHC |
Description
Description: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). CK8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin 8 and 18. CK8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. Anti-CK8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against CK8, CK18 and CK19). Reportedly, anti-CK8 is useful for the differentiation of lobular ( ring-like, perinuclear ) from ductal ( peripheral-predominant ) carcinoma of the breast.
Additional Text
Gene Name
KRT8
Gene ID
3856
Uniprot ID
P05787
Purification
Protein A/G Purified
Antibody Clonality
Monoclonal
Application Notes
Western Blot (1-2µg/ml); Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Storage Note
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
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