Anti-Filaggrin (Keratinocyte Differentiation Marker) Recombinant Mouse Monoclonal Antibody (Clone:rFLG/1561)

Catalogue Number: 12-1180-ABO

Manufacturer:Abeomics
Shelf Life:24 months
Type:Recombinant Monoclonal
Alias:ATOD2; Epidermal Filaggrin; Filaggrin; Filaggrin precursor; Fillagrin; FLG; Profilaggrin
Shipping Condition:Blue Ice
Unit(s): 100 ug, 20 ug
Host name: Mouse
Clone: rFLG/1561
Isotype: IgG1, kappa
Immunogen: Recombinant fragment of human Filaggrin protein (aa 198-288) (exact sequence is proprietary)
Application: IHC

Description

Description: Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, sµggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.

Additional Text

Gene Name

Flg

Purification

Protein A/G Purified

Gene ID

2312

Uniprot ID

P20930

Antibody Clonality

Monoclonal

Application Notes

Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)

Storage Note

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.