Anti-GFAP (Astrocyte & Neural Stem Cell Marker) Recombinant Mouse Monoclonal Antibody (Clone:rASTRO/789)

Catalogue Number: 12-1184-ABO

Manufacturer:Abeomics
Shelf Life:24 months
Type:Recombinant Monoclonal
Alias:Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein (GFAP)
Shipping Condition:Blue Ice
Unit(s): 100 ug, 20 ug
Host name: Mouse
Clone: rASTRO/789
Isotype: IgG1, kappa
Immunogen: Recombinant full-length human GFAP protein
Application: FACS, WB, IHC

Description

Description: This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Additional Text

Gene Name

GFAP

Gene ID

2670

Purification

Protein A/G Purified

Uniprot ID

P14136

Antibody Clonality

Monoclonal

Application Notes

Flow Cytometry (1-2µg/million cells);Western Blot (1-2µg/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)

Storage Note

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.