Anti-Glycophorin A / CD235a (Erythrocyte Marker) Recombinant Mouse Monoclonal Antibody (Clone:rGYPA/280)

Catalogue Number: 12-1186-ABO

Manufacturer:Abeomics
Shelf Life:24 months
Type:Recombinant Monoclonal
Alias:Blood group--MN locus; GPA; GPErik; GpMiIII; GPSAT; GYPA; MN sialoglycoprotein; MNS; PAS2; Sialoglycoprotein alpha
Shipping Condition:Blue Ice
Unit(s): 100 ug, 20 ug
Host name: Mouse
Clone: rGYPA/280
Isotype: IgG1, kappa
Immunogen: Recombinant full-length human glycophorin A protein
Application: WB, IHC

Description

Description: Recognizes a sialoglycoprotein of 39kDa, identified as glycophorin A (GPA). It is present on red blood cells (RBC) and erythroid precursor cells. It has been shown that glycophorin acts as the receptor for Sandei virus and parvovirus.Glycophorins A (GPA) and B (GPB), which are single, trans-membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

Additional Text

Purification

Protein A/G Purified

Uniprot ID

P02724

Antibody Clonality

Monoclonal

Gene ID

2993; 2994

Application Notes

Western Blot (1-2µg/ml); Immunohistochemistry (Formalin-fixed) (0.25-0.5µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)

Gene Name

GYPA, GYPB

Storage Note

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.