Anti-MyoD1 (Rhabdomyosarcoma Marker) Recombinant Mouse Monoclonal Antibody (Clone:rMYD712)
Catalogue Number: 12-1219-ABO
| Manufacturer: | Abeomics |
| Shelf Life: | 24 months |
| Type: | Recombinant Monoclonal |
| Alias: | bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM |
| Shipping Condition: | Blue Ice |
| Unit(s): | 100 ug, 20 ug |
| Host name: | Mouse |
| Clone: | rMYD712 |
| Isotype: | IgG1, kappa |
| Immunogen: | Recombinant full-length human MyoD1 protein |
| Application: | IHC |
Description
Description: Recognizes a phosphor-protein of 45kDa, identified as MyoD1. This MAb does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.
Additional Text
Gene ID
4654
Gene Name
MYOD1
Uniprot ID
P15172
Purification
Protein A/G Purified
Antibody Clonality
Monoclonal
Application Notes
Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Storage Note
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
12-1219 Datasheet
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