Anti-MyoD1 (Rhabdomyosarcoma Marker) Recombinant Mouse Monoclonal Antibody (Clone:rMYD712)

Catalogue Number: 12-1219-ABO

Manufacturer:Abeomics
Shelf Life:24 months
Type:Recombinant Monoclonal
Alias:bHLHc1, Class C basic helix-loop-helix protein 1, Myoblast determination protein 1, Myogenic differentiation 1, Myogenic factor 3 (Myf-3), Myogenin D1, PUM
Shipping Condition:Blue Ice
Unit(s): 100 ug, 20 ug
Host name: Mouse
Clone: rMYD712
Isotype: IgG1, kappa
Immunogen: Recombinant full-length human MyoD1 protein
Application: IHC

Description

Description: Recognizes a phosphor-protein of 45kDa, identified as MyoD1. This MAb does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.

Additional Text

Gene ID

4654

Gene Name

MYOD1

Uniprot ID

P15172

Purification

Protein A/G Purified

Antibody Clonality

Monoclonal

Application Notes

Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)

Storage Note

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.